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鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�(gu贸)400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ(xu茅)鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗�锛岄儴鍒嗗(xu茅)鏍℃洿鏂拌嚦2012骞达紝2013骞�锛涘潎鎻愪緵鏀惰不(f猫i)涓嬭級銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛�(di菐n)鎿娾€�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛�(di菐n)鎿娾€�鑰冨崥瑭﹀嵎搴�(k霉)鈥� 涓嬭級
2018 骞村寳浜鎶€澶у(xu茅)鎷涙敹纰╁＋鐮旂┒鐢熷叆瀛�(xu茅)鑰冪爺澶х侗
628-鐢熺墿鍖栧(xu茅)
涓€銆佽€冭│鎬ц唱(zh矛)
鐢熺墿宸ョ▼纰╁＋鐮旂┒鐢熺敓鐗╁寲瀛�(xu茅)鍏ュ(xu茅)鑰冭│鏄偤鎴戞牎鐢熺墿宸ョ▼瀛�(xu茅)绉戞嫑鏀剁ⅸ澹爺
绌剁敓鑰岄€�(j矛n)琛岀殑姘村钩鑰冭│銆傞€氶亷(gu貌)瑭查杸(m茅n)瑾茬▼鐨勮€冭│浠ョ湡瀵�(sh铆)鍙嶆槧鑰冪敓灏�(du矛)鐢熺墿鍖栧(xu茅)鍩烘湰姒�
蹇靛拰鍩烘湰鐞嗚珫鐨勬帉鎻＄▼搴︿互鍙婄稖鍚堥亱(y霉n)鐢ㄦ墍瀛�(xu茅)鐨勭煡璀�(sh铆)鍒嗘瀽鐩搁棞(gu膩n)鍟�(w猫n)椤屽拰瑙ｆ焙鍟�(w猫n)椤岀殑
鑳藉姏鑸囨按骞�锛屽彲浠ヤ綔鐐烘垜鏍￠伕鎾ョⅸ澹爺绌剁敓鐨勯噸瑕佷緷鎿�(j霉)銆�
浜屻€佽€冭│瑕佹眰
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鏋愬晱(w猫n)椤�銆佽В姹哄晱(w猫n)椤岀殑鑳藉姏銆�
涓�銆佽€冭│褰㈠紡鑸囪│鍗风祼(ji茅)妲�(g貌u)
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锛堜簩锛夎€冭│鏅�(sh铆)闁擄細180 鍒嗛悩
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鍥涖€佽€冭│鍏�(n猫i)瀹�
鑰冭│鍏�(n猫i)瀹瑰皣娑夊強鐢熺墿鍖栧(xu茅)鐨勫涓嬪収(n猫i)瀹癸細锛�1锛夌敓鐗╁垎瀛愮殑绲�(ji茅)妲�(g貌u)銆佺祫鎴愩€佹€ц唱(zh矛)
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鑳介噺杞�(zhu菐n)鍖栥€佸埄鐢ㄥ拰瑾�(di脿o)绡€(ji茅)锛涳紙4锛夌敓鐗╁ぇ鍒嗗瓙鐨勫垎瑙ｈ垏鍚堟垚浠ｈ瑵锛涳紙5锛夌敓鐗╀俊鎭垎
瀛愮殑寰�(f霉)鍒�銆佽綁(zhu菐n)閷�銆佽〃閬�(d谩)鍜岃(di脿o)绡€(ji茅)绛夊熀鏈悊璜�銆傚苟鑰冩煡瀛�(xu茅)鐢熼亱(y霉n)鐢ㄤ笂杩扮煡璀�(sh铆)鐨勭稖鍚堝拰鍒�
鏋愯兘鍔涖€傚悇閮ㄥ垎鐨勫熀鏈収(n猫i)瀹瑰涓嬶細
鐢熺墿鍒嗗瓙灏�(d菐o)璜�
鐢熺墿绲�(ji茅)妲�(g貌u)涓殑闈炲叡鍍�(ji脿)浣滅敤鍔�
绗竴閮ㄥ垎锛氭皑鍩洪吀鑸囪泲鐧借唱(zh矛)
涓€銆佹皑鍩洪吀
1銆� 姘ㄥ熀閰哥殑绲�(ji茅)妲�(g貌u)鐗归粸(di菐n)鑸囧垎椤�銆佸厜璀滅壒鎬�
2銆� 姘ㄥ熀閰哥殑绛夐浕榛�(di菐n)
浜�銆佽泲鐧借唱(zh矛)鐨勫垎闆㈢磾鍖�
SDS-PAGE銆佷簩缍浕娉�銆佸嚌鑶犻亷(gu貌)婵惧堡鏋�銆佺～閰搁姩娌夋穩娉曘€侀€忔瀽銆侀洟瀛愪氦鎻�
灞ゆ瀽銆佽Κ鍜屽堡鏋愮瓑鐨勫熀鏈師鐞嗚垏鎳�(y墨ng)鐢�
涓�銆佽泲鐧借唱(zh矛)鐨勫叡鍍�(ji脿)绲�(ji茅)妲�(g貌u)
1銆佽偨閸�銆佽偨閺�銆佽偨骞抽潰鐨勬蹇�锛岃胺鑳辩敇鑲�銆佽儼宄剁礌鐨勭祼(ji茅)妲�(g貌u)鐗归粸(di菐n)
2銆佽泲鐧借唱(zh矛)鍥涘€�(g猫)绲�(ji茅)妲�(g貌u)灞ゆ鐨勬蹇�锛屽強缍寔鍚勭礆(j铆)绲�(ji茅)妲�(g貌u)鐨勫寲瀛�(xu茅)閸�
3銆佹皑鍩洪吀搴忓垪娓�(c猫)瀹�锛屽吀鍨嬭泲鐧介叾鐨勪綔鐢ㄤ綅榛�(di菐n)
鍥涖€佽泲鐧借唱(zh矛)鐨勪笁缍祼(ji茅)妲�(g貌u)
1銆佸洓绋熀鏈簩绱�(j铆)绲�(ji茅)妲�(g貌u)椤炲瀷鐨勬蹇靛強绲�(ji茅)妲�(g貌u)鐗归粸(di菐n)
2銆佺祼(ji茅)妲�(g貌u)鍩熴€佸姛鑳藉煙銆佷簽鍩虹殑鍩烘湰姒傚康
3銆佽泲鐧借唱(zh矛)鐨勮畩鎬ц垏寰�(f霉)鎬�銆佸垎瀛愪即渚�
浜�銆佽泲鐧借唱(zh矛)鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳�
1銆佽倢绱呰泲鐧藉拰琛€绱呰泲鐧界殑姘у悎鏇茬窔
2銆佸垾妲�(g貌u)鏁堟噳(y墨ng)锛孊ohr 鏁堟噳(y墨ng)锛孊PG 灏�(du矛)姘у悎鏇茬窔褰遍熆
3銆丠bS 鐨勫垎瀛愭(j墨)鍒�
4銆両gG 鐨勫熀鏈祼(ji茅)妲�(g貌u)
5銆佸鍏嬮殕鎶楅珨銆佸柈鍏嬮殕鎶楅珨銆丒LISA銆乄estern blotting銆佸厤鐤Κ鍜屽堡鏋�
6銆佽倢鐞冭泲鐧借垏鑲屽嫊(d貌ng)铔嬬櫧鐨勬(g貌u)璞″強婊戝嫊(d貌ng)绲叉ā鍨�
绗簩閮ㄥ垎锛氶叾
涓€銆佸熀鏈蹇碉細杓旈叾銆佽紨鍔╁洜瀛�銆佹瘮娲诲害銆佹牳閰�銆侀叾鐨勬椿鎬т腑蹇�銆侀叾鍘�銆�
鍚屽姛閰剁瓑銆�
浜屻€侀叾鐨勬椿鍔涙脯(c猫)瀹�
涓�銆侀叾淇冨弽鎳�(y墨ng)鍕�(d貌ng)鍔涘(xu茅)
1銆佸簳鐗╁皪(du矛)閰朵績鍙嶆噳(y墨ng)閫熷害鐨勫奖闊匡細绫冲紡鏂圭▼锛孠m銆乂max 鐨勬剰缇�銆�
2銆佹韩搴�銆乸H 鍊煎皪(du矛)閰朵績鍙嶆噳(y墨ng)閫熷害鐨勫奖闊�銆�
3銆佹姂鍒跺姂灏�(du矛)閰朵績鍙嶆噳(y墨ng)閫熷害鐨勫奖闊�銆傛姂鍒朵綔鐢ㄩ鍨�锛屽彲閫嗘姂鍒朵綔鐢ㄧ殑鍕�(d貌ng)
鍔涘(xu茅)鏇茬窔锛屻€�
鍥�銆侀叾鐨勪綔鐢ㄦ(j墨)鍒�
1銆佽獦灏�(d菐o)濂戝悎瀛�(xu茅)瑾�(shu艒)銆佸偓鍖栦笁鑱�(li谩n)楂�
2銆佸奖闊块叾鍌寲鏁堢巼鐨勬湁闂�(gu膩n)鍥犵礌
3銆侀叾娲绘€х殑瑾�(di脿o)鎺ф(j墨)鍒讹紙鍒ユ(g貌u)瑾�(di脿o)鎺�锛岄叾鍘熸縺娲伙紝鍏卞児(ji脿)瑾�(di脿o)鎺�锛岄叾鍚噺鐨勮(di脿o)
绡€(ji茅)锛�
4銆佸悓鍔熼叾
绗笁閮ㄥ垎锛氭牳閰稿強婵€绱�
1銆佹牳閰哥殑鍖栧(xu茅)绲勬垚
2銆丏NA 鐨勯洐铻烘棆绲�(ji茅)妲�(g貌u)妯″瀷
3銆佸吀鍨� RNA 鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳�
4銆丏NA 鐨勮畩鎬�锛岃畩鎬у悗鐨勬€ц唱(zh矛)鏀硅畩
5銆丏NA 鐨勫京(f霉)鎬�銆佸垎瀛愰洔浜�锛孲outhern blot 鍜� Northern blot
6銆侀噸瑕佹縺绱犵殑鍖栧(xu茅)鏈唱(zh矛)鍙婂姛鑳�
7銆佹縺绱犵殑浣滅敤姗�(j墨)鍒�锛岀浜屼俊浣垮(xu茅)瑾�(shu艒)
绗洓閮ㄥ垎锛氱硸浠ｈ瑵
1銆佺硸閰佃В
1.1銆佺啛瑷橀叺瑙ｉ€斿緫涓殑鍚勬閰朵績鍙嶆噳(y墨ng)
1.2銆佸繁绯栨縺閰讹紙钁¤悇绯栨縺閰讹級銆�6-纾烽吀鏋滅硸婵€閰�-1銆佷笝閰吀婵€閰剁殑瑾�(di脿o)绡€(ji茅)
1.3銆侀叺瑙ｉ亷(gu貌)绋嬭兘閲忚▓(j矛)绠�
1.4銆丆 鐨勫幓鍚�
2銆佹妾吀寰挵(hu谩n)
2.1銆佺啛瑷樻簴(zh菙n)鍌欓殠娈靛拰妾告閰稿惊鐠�(hu谩n)闅庢涓殑鍚勬閰朵績鍙嶆噳(y墨ng)锛屼互鍙婂悇姝ュ弽鎳�(y墨ng)
閰剁殑浣滅敤鐗归粸(di菐n)
2.2銆佹妾吀寰挵(hu谩n)鐨勮(di脿o)鎺э紝鑽夐叞涔欓吀鐨勫洖瑁�(b菙)鍙嶆噳(y墨ng)
2.3銆佷唬璎濈墿閫�(j矛n)鍑烘妾吀寰挵(hu谩n)璺緫
2.4銆佽兘閲忚▓(j矛)绠�
2.5銆丆 鐨勫幓鍚�
3銆佹哀鍖栫７閰稿寲
3.1銆佸熀鏈蹇碉細闆诲瓙閺堛€丵 寰挵(hu谩n)銆佽В鍋惰伅(li谩n)鍔戙€佸簳鐗╂按骞崇７閰稿寲
3.2銆佺窔绮掗珨闆诲瓙鍌抽仦閺堬紙闆诲瓙鍦ㄥ京(f霉)鍚堥珨鍜岄仦闆诲瓙楂斾箣闁撶殑鍌抽仦娆″簭锛�
3.3銆丄TP 鐨勫悎鎴愭(j墨)鍒�
3.4銆丯ADH 鐨勭┛姊(j墨)鍒�
4銆佹垔绯栫７閰搁€斿緫姘у寲闅庢鐨勯噸瑕佷唬璎濅腑闁撶墿鍙婄敓鐞嗘剰缇�
5銆佺硸鐣扮敓浣滅敤鍩烘湰姒傚康鍙婅垏绯栭叺瑙ｉ亷(gu貌)绋嬬殑鐣板悓
6銆佺硸鍘熺殑鍒嗚В鑸囧悎鎴愪唬璎濋亷(gu貌)绋嬬殑鍙嶆噳(y墨ng)鍙婅兘閲忚▓(j矛)绠�
绗簲閮ㄥ垎锛氳剛椤炰唬璎�
1銆� 鑴傝偑閰哥殑娲诲寲銆佽綁(zhu菐n)閬�(y霉n)銆佄� 姘у寲
2銆� 鑴傝偑閰搁檷瑙ｇ殑鑳介噺瑷�(j矛)绠�
3銆侀叜楂旂殑姒傚康銆佺敓鎴愬強鍒╃敤
4銆佽剛鑲吀鐨勫悎鎴�
5銆佽剛鑲吀鍒嗚В浠ｈ瑵鑸囧悎鎴愪唬璎濈殑瑾�(di脿o)鎺�
绗叚閮ㄥ垎锛氭煋鑹查珨鑸� DNA
1銆� 鏌撹壊楂�
2銆� DNA 鐨勭祼(ji茅)妲�(g貌u)
3銆� DNA 鐨勫京(f霉)鍒�
4銆� 鍘熸牳鐢熺墿鍜岀湡鏍哥敓鐗� DNA 寰�(f霉)鍒剁壒榛�(di菐n)
5銆� DNA 鐨勪慨寰�(f霉)
6銆� DNA 鐨勮綁(zhu菐n)搴�
绗竷閮ㄥ垎锛氱敓鐗╀俊鎭殑鍌抽仦 RNA 鐨勮綁(zhu菐n)閷�
1銆� 鍟熷嫊(d貌ng)瀛愯垏杞�(zhu菐n)閷勮捣濮�
2銆� 鍘熸牳鐢熺墿鑸囩湡鏍哥敓鐗� mRNA 鐨勭壒寰佹瘮杓�
3銆� 绲傛鑸囩祩姝㈠瓙
4銆� 绗簲绡€(ji茅)鍏�(n猫i)鍚瓙鐨勫壀鎺�銆佺法杓強鍖栧(xu茅)淇＞
5銆� mRNA 鍙婇伜鍌冲瘑纰�---涓夎伅(li谩n)瀛�
6銆� tRNA
7銆� 鏍哥硸楂�
8銆� 铔嬬櫧璩�(zh矛)鐨勫悎鎴�
9銆� 铔嬬櫧璩�(zh矛)鐨勯亱(y霉n)杞�(zhu菐n)
绗叓閮ㄥ垎锛氬垎瀛愮敓鐗╁(xu茅)鐮旂┒鏂规硶
1銆� 閲嶇祫 DNA 鎶€琛�(sh霉)鍥為¨
2銆� DNA 鍩烘湰鎿嶄綔鎶€琛�(sh霉)
3銆� RNA 鍩烘湰鎿嶄綔鎶€琛�(sh霉)
4銆� 鍩哄洜鍏嬮殕鎶€琛�(sh霉)
5銆� 铔嬬櫧璩�(zh矛)绲勫(xu茅)鑸囪泲鐧借唱(zh矛)绲勫(xu茅)鎶€琛�(sh霉)
6銆� 鍩哄洜琛ㄩ仈(d谩)鐮旂┒鎶€琛�(sh霉)
7銆� 鍩哄洜鏁查櫎鎶€琛�(sh霉)
8銆� 铔嬬櫧璩�(zh矛)鑸� RNA 鐩镐簰浣滅敤鎶€琛�(sh霉)
9銆� 鍩哄洜琛ㄩ仈(d谩)璀滃垎鏋�
10銆侀叺姣嶅姛鑳戒簰瑁�(b菙)閼掑畾
11銆佸熀鍥犺〃閬�(d谩)鐮旂┒鎶€琛�(sh霉)
绗節閮ㄥ垎锛氬熀鍥犵殑琛ㄩ仈(d谩)鑸囪(di脿o)鎺�
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