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绗竴閮ㄥ垎锛氭皑鍩洪吀鑸囪泲鐧借唱(zh矛)
涓€銆佹皑鍩洪吀
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涓�銆佽泲鐧借唱(zh矛)鐨勫叡鍍圭祼(ji茅)妲�(g貌u)
1銆佽偨閸�銆佽偨閺�銆佽偨骞抽潰鐨勬蹇�锛岃胺鑳辩敇鑲�銆佽儼宄剁礌鐨勭祼(ji茅)妲�(g貌u)鐗归粸
2銆佽泲鐧借唱(zh矛)鍥涘€嬬祼(ji茅)妲�(g貌u)灞ゆ鐨勬蹇�锛屽強缍寔鍚勭礆绲�(ji茅)妲�(g貌u)鐨勫寲瀛�(xu茅)閸�
3銆佹皑鍩洪吀搴忓垪娓畾锛屽吀鍨嬭泲鐧介叾鐨勪綔鐢ㄤ綅榛�
鍥�銆佽泲鐧借唱(zh矛)鐨勪笁缍祼(ji茅)妲�(g貌u)
1銆佸洓绋熀鏈簩绱氱祼(ji茅)妲�(g貌u)椤炲瀷鐨勬蹇靛強绲�(ji茅)妲�(g貌u)鐗归粸
2銆佺祼(ji茅)妲�(g貌u)鍩�銆佸姛鑳藉煙銆佷簽鍩虹殑鍩烘湰姒傚康
3銆佽泲鐧借唱(zh矛)鐨勮畩鎬ц垏寰�(f霉)鎬с€佸垎瀛愪即渚�
浜�銆佽泲鐧借唱(zh矛)鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳�
1銆佽倢绱呰泲鐧藉拰琛€绱呰泲鐧界殑姘у悎鏇茬窔
2銆佸垾妲�(g貌u)鏁堟噳(y墨ng)锛孊ohr 鏁堟噳(y墨ng)锛孊PG 灏嶆哀鍚堟洸绶氬奖闊�
3銆丠bS 鐨勫垎瀛愭(j墨)鍒�
4銆両gG 鐨勫熀鏈祼(ji茅)妲�(g貌u)
5銆佸鍏嬮殕鎶楅珨銆佸柈鍏嬮殕鎶楅珨銆丒LISA銆乄estern blotting銆佸厤鐤Κ鍜屽堡鏋�
6銆佽倢鐞冭泲鐧借垏鑲屽嫊铔嬬櫧鐨勬(g貌u)璞″強婊戝嫊绲叉ā鍨�
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涓夈€侀叾淇冨弽鎳�(y墨ng)鍕曞姏瀛�(xu茅)
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2銆佹韩搴�銆乸H 鍊煎皪閰朵績鍙嶆噳(y墨ng)閫熷害鐨勫奖闊�銆�
3銆佹姂鍒跺姂灏嶉叾淇冨弽鎳�(y墨ng)閫熷害鐨勫奖闊�銆傛姂鍒朵綔鐢ㄩ鍨�锛屽彲閫嗘姂鍒朵綔鐢ㄧ殑鍕�
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鍥�銆侀叾鐨勪綔鐢ㄦ(j墨)鍒�
1銆佽獦灏�(d菐o)濂戝悎瀛�(xu茅)瑾�銆佸偓鍖栦笁鑱�(li谩n)楂�
2銆佸奖闊块叾鍌寲鏁堢巼鐨勬湁闂�(gu膩n)鍥犵礌
3銆侀叾娲绘€х殑瑾�(di脿o)鎺ф(j墨)鍒讹紙鍒ユ(g貌u)瑾�(di脿o)鎺�锛岄叾鍘熸縺娲�锛屽叡鍍硅(di脿o)鎺�锛岄叾鍚噺鐨勮(di脿o)
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4銆佸悓鍔熼叾
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1銆佹牳閰哥殑鍖栧(xu茅)绲勬垚
2銆丏NA 鐨勯洐铻烘棆绲�(ji茅)妲�(g貌u)妯″瀷
3銆佸吀鍨� RNA 鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳�
4銆丏NA 鐨勮畩鎬�锛岃畩鎬у悗鐨勬€ц唱(zh矛)鏀硅畩
5銆丏NA 鐨勫京(f霉)鎬�銆佸垎瀛愰洔浜�锛孲outhern blot 鍜� Northern blot
6銆侀噸瑕佹縺绱犵殑鍖栧(xu茅)鏈唱(zh矛)鍙婂姛鑳�
7銆佹縺绱犵殑浣滅敤姗�(j墨)鍒�锛岀浜屼俊浣垮(xu茅)瑾�
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1銆佺硸閰佃В
1.1銆佺啛瑷橀叺瑙ｉ€斿緫涓殑鍚勬閰朵績鍙嶆噳(y墨ng)
1.2銆佸繁绯栨縺閰讹紙钁¤悇绯栨縺閰讹級銆�6-纾烽吀鏋滅硸婵€閰�-1銆佷笝閰吀婵€閰剁殑瑾�(di脿o)绡€(ji茅)
1.3銆侀叺瑙ｉ亷绋嬭兘閲忚▓绠�
1.4銆丆 鐨勫幓鍚�
2銆佹妾吀寰挵(hu谩n)
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閰剁殑浣滅敤鐗归粸
2.2銆佹妾吀寰挵(hu谩n)鐨勮(di脿o)鎺�锛岃崏閰颁箼閰哥殑鍥炶鍙嶆噳(y墨ng)
2.3銆佷唬璎濈墿閫�(j矛n)鍑烘妾吀寰挵(hu谩n)璺緫
2.4銆佽兘閲忚▓绠�
2.5銆丆 鐨勫幓鍚�
3銆佹哀鍖栫７閰稿寲
3.1銆佸熀鏈蹇碉細闆诲瓙閺堛€丵 寰挵(hu谩n)銆佽В鍋惰伅(li谩n)鍔�銆佸簳鐗╂按骞崇７閰稿寲
3.2銆佺窔绮掗珨闆诲瓙鍌抽仦閺堬紙闆诲瓙鍦ㄥ京(f霉)鍚堥珨鍜岄仦闆诲瓙楂斾箣闁撶殑鍌抽仦娆″簭锛�
3.3銆丄TP 鐨勫悎鎴愭(j墨)鍒�
3.4銆丯ADH 鐨勭┛姊(j墨)鍒�
4銆佹垔绯栫７閰搁€斿緫姘у寲闅庢鐨勯噸瑕佷唬璎濅腑闁撶墿鍙婄敓鐞嗘剰缇�
5銆佺硸鐣扮敓浣滅敤鍩烘湰姒傚康鍙婅垏绯栭叺瑙ｉ亷绋嬬殑鐣板悓
6銆佺硸鍘熺殑鍒嗚В鑸囧悎鎴愪唬璎濋亷绋嬬殑鍙嶆噳(y墨ng)鍙婅兘閲忚▓绠�
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1銆� 鑴傝偑閰哥殑娲诲寲銆佽綁(zhu菐n)閬�銆佄� 姘у寲
2銆� 鑴傝偑閰搁檷瑙ｇ殑鑳介噺瑷堢畻
3銆侀叜楂旂殑姒傚康銆佺敓鎴愬強鍒╃敤
4銆佽剛鑲吀鐨勫悎鎴�
5銆佽剛鑲吀鍒嗚В浠ｈ瑵鑸囧悎鎴愪唬璎濈殑瑾�(di脿o)鎺�
绗叚閮ㄥ垎锛氭煋鑹查珨鑸� DNA
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2銆� DNA 鐨勭祼(ji茅)妲�(g貌u)
3銆� DNA 鐨勫京(f霉)鍒�
4銆� 鍘熸牳鐢熺墿鍜岀湡鏍哥敓鐗� DNA 寰�(f霉)鍒剁壒榛�
5銆� DNA 鐨勪慨寰�(f霉)
6銆� DNA 鐨勮綁(zhu菐n)搴�
绗竷閮ㄥ垎锛氱敓鐗╀俊鎭殑鍌抽仦 RNA 鐨勮綁(zhu菐n)閷�
1銆� 鍟熷嫊瀛愯垏杞�(zhu菐n)閷勮捣濮�
2銆� 鍘熸牳鐢熺墿鑸囩湡鏍哥敓鐗� mRNA 鐨勭壒寰佹瘮杓�
3銆� 绲傛鑸囩祩姝㈠瓙
4銆� 绗簲绡€(ji茅)鍏�(n猫i)鍚瓙鐨勫壀鎺ャ€佺法杓強鍖栧(xu茅)淇＞
5銆� mRNA 鍙婇伜鍌冲瘑纰�---涓夎伅(li谩n)瀛�
6銆� tRNA
7銆� 鏍哥硸楂�
8銆� 铔嬬櫧璩�(zh矛)鐨勫悎鎴�
9銆� 铔嬬櫧璩�(zh矛)鐨勯亱杞�(zhu菐n)
绗叓閮ㄥ垎锛氬垎瀛愮敓鐗╁(xu茅)鐮旂┒鏂规硶
1銆� 閲嶇祫 DNA 鎶€琛�(sh霉)鍥為¨
2銆� DNA 鍩烘湰鎿嶄綔鎶€琛�(sh霉)
3銆� RNA 鍩烘湰鎿嶄綔鎶€琛�(sh霉)
4銆� 鍩哄洜鍏嬮殕鎶€琛�(sh霉)
5銆� 铔嬬櫧璩�(zh矛)绲勫(xu茅)鑸囪泲鐧借唱(zh矛)绲勫(xu茅)鎶€琛�(sh霉)
6銆� 鍩哄洜琛ㄩ仈(d谩)鐮旂┒鎶€琛�(sh霉)
7銆� 鍩哄洜鏁查櫎鎶€琛�(sh霉)
8銆� 铔嬬櫧璩�(zh矛)鑸� RNA 鐩镐簰浣滅敤鎶€琛�(sh霉)
9銆� 鍩哄洜琛ㄩ仈(d谩)璀滃垎鏋�
10銆侀叺姣嶅姛鑳戒簰瑁滈憭瀹�
11銆佸熀鍥犺〃閬�(d谩)鐮旂┒鎶€琛�(sh霉)
绗節閮ㄥ垎锛氬熀鍥犵殑琛ㄩ仈(d谩)鑸囪(di脿o)鎺�
1銆� 鍘熸牳鍩哄洜瑾�(di脿o)鎺х附璜�
2銆� 涔崇硸鎿嶇副瀛�
3銆� 鑹叉皑閰告搷绺卞瓙
4銆� 杞�(zhu菐n)閷勫悗瑾�(di脿o)鎺�
5銆� 鐪熸牳绱�(x矛)鑳炵殑鍩哄洜绲�(ji茅)妲�(g貌u)
6銆� 闋嗗紡浣滅敤鍏冧欢鑸囧熀鍥犺(di脿o)鎺�
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