閯窞澶у(xu茅)纰╁＋鐮旂┒鐢熷叆瀛�(xu茅)鑰冭│澶х侗

鍩虹(ch菙)閱�(y墨)瀛�(xu茅)缍滃悎鑰冭│澶х侗
1銆� 鑰冭│鑼冨湇锛�
鐢熺悊瀛�(xu茅)銆佺敓鐗╁寲瀛�(xu茅)銆佸厤鐤(xu茅)銆佺梾鐞嗗(xu茅)鍥涢杸瀛�(xu茅)绉戠殑鍩虹(ch菙)鐭ヨ瓨(sh铆)鍜屽熀鏈悊璜�銆�
2銆佽€冭│鐩(bi膩o)鍙婅姹傦細
瑕佹眰鑰冪敓鎺屾彙鐢熺悊瀛�(xu茅)銆佺敓鐗╁寲瀛�(xu茅)銆佸厤鐤(xu茅)銆佺梾鐞嗗(xu茅)鐨勫熀绀�(ch菙)鐞嗚珫锛岃兘澶�
閬�(y霉n)鐢ㄩ€欎簺鐞嗚珫鍒嗘瀽鍟忛銆佽В姹哄晱椤�锛屽叿鍌欐敾璁€纰╁＋瀛�(xu茅)浣嶇爺绌剁敓鐨勫熀鏈礌璩�(zh矛)锛�
閬�(d谩)鍒扮爺绌剁敓鍏ュ(xu茅)姘村钩銆�
3銆佽│椤屽垎鍊硷細300 鍒�
4銆佺瓟椤屾柟寮忓強鏅�(sh铆)闁擄細
闁夊嵎銆佺瓎瑭�銆�180 鍒嗛悩
5銆佸悇绉戞瘮渚嬶細
鐢熺悊瀛�(xu茅)
25%
鐢熺墿鍖栧(xu茅) 25%
鍏嶇柅瀛�(xu茅)
鐥呯悊瀛�(xu茅)
25%
25%
6銆侀鍨嬶細
鍚嶈瑙ｉ噵
24 椤�锛�5 鍒�/椤岋紝鍏� 120 鍒�
60 椤�锛�2 鍒�/椤�锛屽叡 120 鍒�
4 椤�锛�15 鍒�/椤岋紝鍏� 60 鍒�
A 鍨嬮伕鎿囬
鍟忕瓟椤�
锛堥鍨嬭鏄庯細A 鍨嬮鏄寚鍦ㄦ瘡灏忛绲﹀嚭鐨� A銆丅銆丆銆丏 鍥涘€�(g猫)閬搁爡(xi脿ng)涓�锛岃珛(q菒ng)閬稿嚭
涓€闋�(xi脿ng)鏈€绗﹀悎瑭﹂瑕佹眰鐨勩€傦級
7銆佽€冩煡鑼冨湇锛�
鐢熺悊瀛�(xu茅)
绶掕珫:
1.楂旀恫銆佺窗(x矛)鑳炲収(n猫i)娑插拰绱�(x矛)鑳炲娑茬殑鍩烘湰姒傚康
2.姗�(j墨)楂旂殑鍏�(n猫i)鐠�(hu谩n)澧冨拰绌�(w臎n)鎱�(t脿i)
3.鐢熺悊鍔熻兘鐨勮(di脿o)绡€(ji茅)鏂瑰紡锛氱缍�(j墨ng)瑾�(di脿o)绡€(ji茅)銆侀珨娑茶(di脿o)绡€(ji茅)鍜岃嚜韬(di脿o)绡€(ji茅)
绱�(x矛)鑳炵殑鍩烘湰鍔熻兘
1.绱�(x矛)鑳炵殑鐗╄唱(zh矛)杞�(zhu菐n)閬�(y霉n)鍔熻兘锛�?ji菐n)娓劅U(ku貌)鏁�銆佺稉(j墨ng)杓夐珨鍜岀稉(j墨ng)閫氶亾鐨勬槗鍖栨摯(ku貌)鏁�銆佷富鍕�(d貌ng)杞�(zhu菐n)閬�(y霉n)銆佸嚭鑳炲拰鍏ヨ優
2.绱�(x矛)鑳炵殑鑸堝ギ鎬ц垏鐢熺墿闆荤従(xi脿n)璞★細绁炵稉(j墨ng)鍜岄楠艰倢绱�(x矛)鑳炵殑闈滄伅闆讳綅鍜屽嫊(d貌ng)浣滈浕浣嶅強鍏剁啊(ji菐n)瑕佺殑鐢�(ch菐n)鐢�
姗�(j墨)鍒�銆佸埡婵€鍜岄柧鍒烘縺銆佸彲鑸堝ギ绱�(x矛)鑳�(鎴栫祫绻�)銆侀浕绶婂嫉闆讳綅鍜屽眬閮ㄩ浕浣嶃€佸嫊(d貌ng)浣滈浕浣�(鎴栬垐濂�)

 
 
鐨勫紩璧峰拰瀹冨湪鍚屼竴绱�(x矛)鑳炰笂鐨勫偝灏�(d菐o)銆佺缍�(j墨ng)-楠ㄩ鑲屾帴闋檿鐨勮垐濂偝閬�銆侀楠艰倢鐨勬敹绺(j墨)鍒躲€佽垐
濂�-鏀剁府鍋惰伅(li谩n)鍜屽奖闊挎敹绺晥鑳界殑鍥犵礌
琛€娑�
1.琛€娑茬殑绲勬垚鍜岀悊鍖栫壒鎬э細琛€娑茬殑绲勬垚鍜岃閲忓強鍏舵(j墨)鑳�銆�
2.琛€绱�(x矛)鑳炲強鍔熻兘锛氱磪绱�(x矛)鑳炵殑褰㈡厠(t脿i)鑸囩敓鐞嗗姛鑳�銆佺磪绱�(x矛)鑳炵殑鎳告诞绌�(w臎n)瀹氭€с€佹徊閫忚剢鎬�銆佺磪绱�(x矛)鑳炵殑
閬�(y霉n)杓稿姛鑳界瓑锛�
3. 娣嬪反绱�(x矛)鑳炵殑鍔熻兘鍜岀壒榛�(di菐n)锛涜灏忔澘鐨勭壒鎬у強鍔熻兘
4. 鐢熺悊鎬ф琛€閬庣▼
5. 琛€娑茬殑鍑濆浐鍜岀簴缍泲鐧芥憾瑙ｏ細澶栨簮鎬у嚌琛€鍜屽収(n猫i)婧愭€у嚌琛€鐨勪富瑕佹椹熷拰涓昏閫斿緫锛岃娑插嚌
鍥虹殑鍩烘湰閬庣▼; 鎶楀嚌绯荤当(t菕ng)涓富瑕佹姉鍑濆洜瀛愬強鍏朵綔鐢�锛岀簴缍泲鐧芥憾瑙ｅ拰鎶楃簴婧剁殑閫斿緫鍜屾椹�
6.琛€鍨嬶細绱呯窗(x矛)鑳炲嚌闆嗚垏琛€鍨嬩箣闁撶殑闂�(gu膩n)绯伙紝杓歌鍘熷墖鍜屼氦鍙夐厤琛€
琛€娑插惊鐠�(hu谩n)
1.蹇冭倢璺ㄨ啘闆讳綅鐢�(ch菐n)鐢熺殑姗�(j墨)鍒朵互鍙婂績鑲岀殑鐢熺悊鐗规€�
2.蹇冭嚐鐨勬车琛€鍔熻兘锛氬績鍕�(d貌ng)鍛ㄦ湡锛屽績鑷熸车琛€鐨勯亷绋嬪拰姗�(j墨)鍒�锛屽績闊筹紝蹇冭嚐娉佃鍔熻兘鐨勮⿻(p铆ng)瀹�锛屽奖
闊垮績杓稿嚭閲忕殑鍥犵礌
3.琛€绠＄敓鐞嗭細琛€绠＄殑鍒嗛鍜岀祼(ji茅)妲�(g貌u)銆佸姛鑳界壒榛�(di菐n)锛岃娴侀噺銆佽娴侀樆鍔涘拰琛€澹�锛屽奖闊胯澹撶殑鍥犵礌锛�
寰惊鐠�(hu谩n)鑸囩墿璩�(zh矛)浜ゆ彌锛岀祫绻旀恫鍜屾穻宸寸殑鐢熸垚鍜屽洖娴佷互鍙婂奖闊垮洜绱�
4. 蹇冭绠℃椿鍕�(d貌ng)鐨勮(di脿o)绡€(ji茅)锛氬績鑷熺殑绁炵稉(j墨ng)鏀厤鍙婂叾浣滅敤銆佽绠＄殑绁炵稉(j墨ng)鏀厤鍙婂叾浣滅敤銆佸績琛€绠℃椿鍕�(d貌ng)
鐨勮(di脿o)绡€(ji茅)銆侀珨娑插洜绱犵殑瑾�(di脿o)绡€(ji茅)
鍛煎惛鐢熺悊
1.鑲洪€氭埃锛氳偤閫氭埃鐨勫嫊(d貌ng)鍔涘拰闃诲姏銆佽兏鑶滆厰鍏�(n猫i)澹�銆佽偤瀹圭⿳鍜岃偤瀹归噺銆佽偤閫氭埃閲忓拰鑲烘场閫氭埃閲�銆�
鑲鸿〃闈㈡椿鎬х墿璩�(zh矛)
2.鑲烘彌姘ｈ垏绲勭箶鎻涙埃锛氳偤鎻涙埃鐨勫熀鏈師鐞�銆侀亷绋嬪拰褰遍熆鍥犵礌锛涢€氭埃/琛€娴佹瘮鍊煎強鍏舵剰缇�
3.姘ｉ珨鍦ㄨ娑蹭腑鐨勯亱(y霉n)杓革細姘у拰浜屾哀鍖栫⒊鍦ㄨ娑蹭腑瀛樺湪鐨勫舰寮�锛屾哀瑙ｉ洟鏇茬窔鍙婂叾褰遍熆鍥犵礌
娑堝寲鍜屽惛鏀�
1.娑堝寲鑸囧惛鏀剁殑姒傚康锛屾秷鍖栫殑鏂瑰紡鍙婂叾鐗归粸(di菐n)锛屾秷鍖栭亾骞虫粦鑲岀殑鐢熺悊鐗规€�
2.鑳冩秷鍖栵細鑳冩恫鐨勬€ц唱(zh矛)銆佹垚鍒嗗拰浣滅敤锛岃儍娑插垎娉屽強鍏惰(di脿o)绡€(ji茅)锛岃儍鐨勯亱(y霉n)鍕�(d貌ng)鍙婂叾瑾�(di脿o)绡€(ji茅)锛岃儍鎺掔┖鐨�
姒傚康鍜屾剰缇�
3.灏忚吀娑堝寲锛氳儼娑茬殑鐢熺悊浣滅敤鍙婂叾鍒嗘硨瑾�(di脿o)绡€(ji茅)銆佽喗姹佺殑鐢熺悊浣滅敤鍙婂叾鍒嗘硨瑾�(di脿o)绡€(ji茅)銆佸皬鑵搁亱(y霉n)鍕�(d貌ng)鏂�
寮�
灏跨殑鐢熸垚鍜屾帓鍑�
1.鑵庡皬鐞冪殑婵鹃亷浣滅敤鍙婂叾褰遍熆鍥犵礌
2.鑵庡皬绠″拰闆嗗悎绠＄殑娉屽翱鍔熻兘

 
 
3.鑵庢硨灏垮姛鑳界殑瑾�(di脿o)绡€(ji茅)锛氭姉鍒╁翱婵€绱犵殑浣滅敤鍙婂叾鍒嗘硨瑾�(di脿o)绡€(ji茅)銆侀啗鍥洪叜鐨勪綔鐢ㄥ強鍏跺垎娉岃(di脿o)绡€(ji茅)銆佽厧
绱�-琛€绠＄穵寮电礌-閱涘浐閰郴绲�(t菕ng)鐨勮(di脿o)绡€(ji茅)
4. 鑵庢竻闄ょ巼鐨勬蹇靛強鍏舵脯(c猫)瀹氱殑鎰忕京
绁炵稉(j墨ng)绯荤当(t菕ng)
1.绁炵稉(j墨ng)鍏冪殑绲�(ji茅)妲�(g貌u)鍜屽姛鑳�
2.绁炵稉(j墨ng)绾栫董鍌冲皫(d菐o)鑸堝ギ鐨勭壒寰�銆佺缍�(j墨ng)绾栫董鐨勮桓婕块亱(y霉n)杓告椿鍕�(d貌ng)鐨勪竴鑸(gu墨)寰�
3.绐佽Ц鍌抽仦锛氳垐濂€х獊瑙稿悗闆讳綅鍜屾姂鍒舵€х獊瑙稿悗闆讳綅鐨勬蹇�锛岀獊瑙稿偝閬炵殑閬庣▼銆佺壒榛�(di菐n)鍜屽師
鐞�
4.绁炵稉(j墨ng)閬炶唱(zh矛)鍜岀缍�(j墨ng)瑾�(di脿o)璩�(zh矛)鐨勬蹇�锛岄仦璩�(zh矛)鍏卞瓨鍙婂叾鎰忕京銆傚懆鍦嶇缍�(j墨ng)绯荤当(t菕ng)涓殑涔欓叞鑶藉牽銆佸幓鐢茶厧
涓婅吅绱犲強鍏剁浉鎳�(y墨ng)鐨勫彈楂�銆�
5.涓鎶戝埗锛氱獊瑙稿悗鎶戝埗鍜岀獊瑙稿墠鎶戝埗鐨勬蹇靛強鍘熺悊
6.绁炵稉(j墨ng)绯荤当(t菕ng)鐨勬劅瑕哄姛鑳斤細鎰熷彈鍣ㄥ強涓€鑸敓鐞嗙壒寰�锛岀壒鐣版€ф姇灏勭郴绲�(t菕ng)鍜岄潪鐗圭暟鎬ф姇灏勭郴绲�(t菕ng)鐨�
姒傚康鍙婂崁(q奴)鍒�锛岃叇骞茬恫(w菐ng)鐙€绲�(ji茅)妲�(g貌u)鐨勪笂琛屾縺娲荤郴绲�(t菕ng)
7.涓绁炵稉(j墨ng)绯荤当(t菕ng)灏�(du矛)杌€楂旈亱(y霉n)鍕�(d貌ng)鐨勮(di脿o)绡€(ji茅)锛氳剨浼戝厠銆佺壗寮靛弽灏�銆佸幓澶ц叇鍍电洿鐨勬蹇碉紝鍩哄簳绁炵稉(j墨ng)绡€(ji茅)
灏�(du矛)杌€楂旈亱(y霉n)鍕�(d貌ng)鐨勮(di脿o)绡€(ji茅)銆佸皬鑵﹀幓杌€楂旈亱(y霉n)鍕�(d貌ng)鐨勮(di脿o)绡€(ji茅)銆侀寪楂旂郴鍜岄寪楂斿绯诲皪(du矛)杌€楂旈亱(y霉n)鍕�(d貌ng)鐨勮(di脿o)绡€(ji茅)銆佸ぇ鑵�
鐨唱(zh矛)灏�(du矛)杌€楂旈亱(y霉n)鍕�(d貌ng)鐨勮(di脿o)绡€(ji茅)
8.涓绁炵稉(j墨ng)绯荤当(t菕ng)灏�(du矛)鍏�(n猫i)鑷熸椿鍕�(d貌ng)鐨勮(di脿o)绡€(ji茅)锛氫氦鎰熺缍�(j墨ng)鍜屽壇浜ゆ劅绁炵稉(j墨ng)绯荤当(t菕ng)鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳界壒寰�
鍏�(n猫i)鍒嗘硨
1.婵€绱狅細婵€绱犵殑姒傚康鍜屽垎椤炪€佷竴鑸壒寰佸強鍏朵綔鐢ㄧ殑鏂瑰紡銆佹(j墨)鍒讹紝浠ュ強婵€绱犵殑鍒嗘硨瑾�(di脿o)绡€(ji茅)
2.涓嬩笜鑵﹀拰鍨傞珨锛氫笅涓樿叇鐨勫垎娉屽姛鑳�銆佸瀭楂旀縺绱犵殑鐢熺悊浣滅敤銆佽吅鍨傞珨婵€绱犲垎娉岀殑瑾�(di脿o)绡€(ji茅)
3.鐢茬媭鑵烘縺绱犵殑鐢熺悊浣滅敤鑸囧垎娉岃(di脿o)绡€(ji茅)
4.鐢茬媭鏃佽吅绱�銆侀檷閳ｇ礌鍜�1锛�25-浜岀茎缍敓绱燚3 鐨勭敓鐞嗕綔鐢ㄥ強鍏跺垎娉岃(di脿o)绡€(ji茅)
5.鑵庝笂鑵猴細鑵庝笂鑵虹毊璩�(zh矛)婵€绱犮€侀菇鐨唱(zh矛)婵€绱�銆佽厧涓婅吅楂撹唱(zh矛)婵€绱犵殑鐢熺悊浣滅敤鍙婂叾鍒嗘硨瑾�(di脿o)绡€(ji茅)銆�
鐢熺墿鍖栧(xu茅)
绗竴绔� 铔嬬櫧璩�(zh矛)鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳�
1. 铔嬬櫧璩�(zh矛)鐨勫垎瀛愮祫鎴�: 姘ㄥ熀閰革細L-伪-姘ㄥ熀閰哥祼(ji茅)妲�(g貌u)閫氬紡鍜屽垎椤�銆�20 绋皑鍩洪吀鐨勮嫳鏂囧悕瑭炲強
绺绗﹁櫉(h脿o)銆佹皑鍩洪吀鐨勭悊鍖栨€ц唱(zh矛)銆傝偨锛氳偨閸佃垏鑲介張锛岃偨鑸囪泲鐧借唱(zh矛)鐨勫崁(q奴)鍒�锛岀敓鐗╂椿鎬ц偨銆�
2. 铔嬬櫧璩�(zh矛)鐨勫垎瀛愮祼(ji茅)妲�(g貌u): 铔嬬櫧璩�(zh矛)鐨勪竴绱�(j铆)绲�(ji茅)妲�(g貌u)锛氱董鎸佷竴绱�(j铆)绲�(ji茅)妲�(g貌u)绌�(w臎n)瀹氱殑鍖栧(xu茅)閸�锛涜泲鐧借唱(zh矛)鐨勪簩绱�(j铆)绲�(ji茅)
妲�(g貌u)锛氳偨鍠厓銆佄�-铻烘棆銆佄�-鎶樼枈銆佄�-杞�(zhu菐n)瑙�銆佺劇瑕�(gu墨)鍗锋洸銆佹ā搴忓強姘ㄥ熀閰稿伌(c猫)閺堝皪(du矛)浜岀礆(j铆)绲�(ji茅)妲�(g貌u)褰㈡垚
鐨勫奖闊�锛涜泲鐧借唱(zh矛)鐨勪笁绱�(j铆)绲�(ji茅)妲�(g貌u)锛氭绱�(j铆)閸点€佺祼(ji茅)妲�(g貌u)鍩熷強鍒嗗瓙浼翠径锛涜泲鐧借唱(zh矛)鐨勫洓绱�(j铆)绲�(ji茅)妲�(g貌u)銆傝泲鐧借唱(zh矛)鐨�
鍒嗛銆�

 
 
3. 铔嬬櫧璩�(zh矛)鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳斤細铔嬬櫧璩�(zh矛)涓€绱�(j铆)绲�(ji茅)妲�(g貌u)鑸囧姛鑳界殑闂�(gu膩n)绯伙細鍒嗗瓙鐥�銆傝泲鐧借唱(zh矛)绌洪枔绲�(ji茅)妲�(g貌u)鑸囧姛鑳�
鐨勯棞(gu膩n)绯伙細铔嬬櫧璩�(zh矛)妲�(g貌u)璞℃敼璁婂拰鐤剧梾銆�
4. 铔嬬櫧璩�(zh矛)鐨勭悊鍖栨€ц唱(zh矛)锛氬叐鎬цВ闆�銆佽啝楂旀€ц唱(zh矛)銆佽泲鐧借唱(zh矛)璁婃€ц垏寰�(f霉)鎬�銆佹矇娣€銆佺传澶栧惛鏀跺弽鎳�(y墨ng)銆�
绗簩绔� 鏍搁吀鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳�
1. 鏍搁吀鐨勫寲瀛�(xu茅)绲勬垚鍙婁竴绱�(j铆)绲�(ji茅)妲�(g貌u)锛氭牳鑻烽吀鐨勭祼(ji茅)妲�(g貌u)锛氬槍鍛よ垏鍢у暥锛屾牳绯栬垏鏍歌嫹锛屾垔绯栫⒊鍘熷瓙鐨�
绶ㄨ櫉(h脿o)銆� 鏍搁吀鐨勪竴绱�(j铆)绲�(ji茅)妲�(g貌u)锛氭蹇�銆佹牳鑻烽吀鍚勭祫鍒嗛枔鐨勯€ｆ帴閸点€佹浉瀵柟寮�銆�
2. DNA 鐨勭┖闁撶祼(ji茅)妲�(g貌u)鑸囧姛鑳斤細DNA 鐨勪簩绱�(j铆)绲�(ji茅)妲�(g貌u)鈥斺€旈洐铻烘棆绲�(ji茅)妲�(g貌u)妯″瀷锛欳hargaff 瑕�(gu墨)鍓�锛� B-DNA
闆欒灪鏃嬬祼(ji茅)妲�(g貌u)妯″瀷瑕侀粸(di菐n)銆侱NA 鐨勮秴铻烘棆绲�(ji茅)妲�(g貌u)鍙婂叾鍦ㄦ煋鑹茶唱(zh矛)涓殑绲勮锛欴NA 鐨勮秴铻烘棆绲�(ji茅)妲�(g貌u)锛屽師鏍�
鐢熺墿 DNA 鐨勯珮绱�(j铆)绲�(ji茅)妲�(g貌u)銆侱NA 鍦ㄧ湡鏍哥敓鐗╃窗(x矛)鑳炴牳鍏�(n猫i)鐨勭祫瑁濓細鏍稿皬楂�銆侱NA 鐨勫姛鑳斤細鍩哄洜 锛屽熀
鍥犵祫锛孌NA 鐨勫姛鑳�銆�
3. RNA 鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳斤細淇′娇 RNA 鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳斤細hnRNA 锛宮RNA 鐨勭祼(ji茅)妲�(g貌u)鐗归粸(di菐n)銆傝綁(zhu菐n)閬�(y霉n) RNA 鐨勭祼(ji茅)
妲�(g貌u)鑸囧姛鑳斤細绋€鏈夊牽鍩�锛岃帠鐠�(hu谩n)绲�(ji茅)妲�(g貌u)锛屾皑鍩洪吀鎺ョ磵鑾�锛屽弽瀵嗙⒓瀛�锛屼笁绱�(j铆)绲�(ji茅)妲�(g貌u)銆傛牳铔嬬櫧楂� RNA 鐨�
绲�(ji茅)妲�(g貌u)鑸囧姛鑳斤細鐪熸牳鍙婂師鏍哥敓鐗╂牳铔嬬櫧楂旂殑绲勬垚銆傚叾浠栧皬鍒嗗瓙 RNA锛氬嫊(d貌ng)鐗╃窗(x矛)鑳炲収(n猫i)鍏朵粬鐨� RNA
绋鍙婂姛鑳�銆�
4. 鏍搁吀鐨勭悊鍖栨€ц唱(zh矛)銆佽畩鎬у拰寰�(f霉)鎬у強鍏舵噳(y墨ng)鐢細鏍搁吀鐨勪竴鑸悊鍖栨€ц唱(zh矛)锛�260nm 绱鍚告敹銆侱NA
鐨勮畩鎬э細姒傚康锛岃В閺堟洸绶�锛孴m 鍊�锛屽鑹叉晥鎳�(y墨ng)銆侱NA 鐨勫京(f霉)鎬ц垏鍒嗗瓙闆滀氦锛氶€€鐏�銆�
绗笁绔�
閰�
1. 閰剁殑鍒嗗瓙绲�(ji茅)妲�(g貌u)鑸囧姛鑳斤細閰剁殑鍒嗗瓙绲勬垚锛�?ji菐n)娓厾绂忣儤Y(ji茅)鍚堥叾锛岄叾铔嬬櫧锛屽叏閰�锛岄噾灞叾锛岃紨
閰�锛岃紨鍩猴紝缍敓绱犺垏杓旈叾锛岀董鐢熺礌鐨勫垎椤炲強鍏惰垏杓旈叾鐨勯棞(gu膩n)绯伙紝甯歌杓旈叾鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳�锛岃紨
閰剁殑浣滅敤锛岄噾灞洟瀛愮殑浣滅敤銆傞叾鐨勬椿鎬т腑蹇冿細蹇呴渶鍩哄湗(tu谩n)锛岀祼(ji茅)鍚堝熀鍦�(tu谩n)锛屽偓鍖栧熀鍦�(tu谩n)銆傚悓宸ラ叾锛�
姒傚康锛孡DH 鍚屽伐閰惰瓬鐨勮畩鍖栧強鎰忕京銆�
2.
姗�(j墨)鍒讹細娲诲寲鑳�锛岃獦灏�(d菐o)濂戝悎鍋囪锛岄劙杩戞晥鎳�(y墨ng)銆佸畾鍚戞帓鍒椼€佸鍏冨偓鍖�銆佽〃闈㈡晥鎳�(y墨ng)銆�
3. 閰朵績鍙嶆噳(y墨ng)鍕�(d貌ng)鍔涘(xu茅)锛� 搴曠墿婵冨害灏�(du矛)鍙嶆噳(y墨ng)閫熷害鐨勫奖闊匡細绫�-鏇兼皬鏂圭▼锛孠m銆乂max銆� 閰舵績
閰朵績鍙嶆噳(y墨ng)鐨勭壒榛�(di菐n)鑸囨(j墨)鍒讹細閰朵績鍙嶆噳(y墨ng)鐨勭壒榛�(di菐n)锛氶珮鏁堟€э紝鐗圭暟鎬�锛屽彲瑾�(di脿o)绡€(ji茅)鎬�銆傞叾淇冨弽鎳�(y墨ng)
搴﹀皪(du矛)鍙嶆噳(y墨ng)閫熷害鐨勫奖闊裤€� 鏈€閬╂韩搴�銆� 鏈€閬� pH銆� 鎶戝埗鍔戝皪(du矛)鍙嶆噳(y墨ng)閫熷害鐨勫奖闊匡細涓嶅彲閫嗘€ф姂
鍒朵綔鐢ㄧ殑鐗归粸(di菐n)锛屽彲閫嗘€ф姂鍒朵綔鐢ㄧ殑绋銆佸崁(q奴)鍒ュ強鍕�(d貌ng)鍔涘(xu茅)鐗归粸(di菐n)銆� 婵€娲诲姂灏�(du矛)鍙嶆噳(y墨ng)閫熷害鐨勫奖闊匡細
蹇呴渶婵€娲诲姂锛岄潪蹇呴渶婵€娲诲姂銆� 閰舵椿鎬ф脯(c猫)瀹氬強閰舵椿鎬у柈浣�銆�
4. 閰剁殑瑾�(di脿o)绡€(ji茅)锛氶叾娲绘€х殑瑾�(di脿o)绡€(ji茅)锛氶叾鍘�锛岄叾鍘熺殑婵€娲荤殑姒傚康銆佹(j墨)鍒跺強鎰忕京銆傝畩妲�(g貌u)閰�锛岃畩妲�(g貌u)瑾�(di脿o)
绡€(ji茅)鑸囧崝(xi茅)鍚屾晥鎳�(y墨ng)銆傞叾鐨勫叡鍍�(ji脿)淇＞瑾�(di脿o)绡€(ji茅)姒傚康銆佺壒榛�(di菐n)鑸囨剰缇�銆傞叾鍚噺鐨勮(di脿o)绡€(ji茅)锛氶叾铔嬬櫧鍚堟垚鐨勮獦灏�(d菐o)
鑸囬樆閬忔蹇碉紝閰堕檷瑙ｇ殑瑾�(di脿o)鎺�銆�
5. 閰剁殑鍛藉悕鑸囧垎椤�銆�
6. 閰惰垏閱�(y墨)瀛�(xu茅)鐨勯棞(gu膩n)绯伙細浜嗚В閰惰垏鐤剧梾鐨勯棞(gu膩n)绯汇€�
绗洓绔� 绯栦唬璎�

 
 
1. 姒傝堪锛氱硸鐨勭敓鐞嗗姛鑳姐€傜硸鐨勬秷鍖栧惛鏀�
姒傛硜銆�
鐗瑰畾杓夐珨杞�(zhu菐n)閬�(y霉n)鐨�銆佷富鍕�(d貌ng)鑰楄兘鐨勯亷绋嬨€傜硸浠ｈ瑵鐨�
2. 绯栫殑鐒℃哀鍒嗚В锛氱硸閰佃В鐨勫弽鎳�(y墨ng)閬庣▼锛氭蹇�锛屽弽鎳�(y墨ng)閬庣▼鍙婅兘閲忕敓鎴�銆傜硸閰佃В鐨勮(di脿o)绡€(ji茅)锛氫笁鍊�(g猫)
闂�(gu膩n)閸甸叾銆傜硸閰佃В鐨勭敓鐞嗘剰缇�銆�
3. 绯栫殑鏈夋哀姘у寲锛氭湁姘ф哀鍖栫殑鍙嶆噳(y墨ng)閬庣▼锛氫笁鍊�(g猫)闅庢锛屼笝閰吀鑴矮閰跺京(f霉)鍚堥珨鐨勭祫鎴愶紝涓夌晶閰�
寰挵(hu谩n)鐨勯亷绋嬪強鐢熺悊鎰忕京銆傛湁姘ф哀鍖栫敓鎴愮殑 ATP 銆傛湁姘ф哀鍖栫殑瑾�(di脿o)绡€(ji茅)锛氫笝閰吀鑴矮閰跺京(f霉)鍚堥珨鍙�
涓夌晶閰稿惊鐠�(hu谩n)涓笁鍊�(g猫)闂�(gu膩n)閸甸叾鐨勮(di脿o)绡€(ji茅)銆傚反鏂痉鏁堟噳(y墨ng)銆�
4. 纾烽吀鎴婄硸閫斿緫锛氱７閰告垔绯栭€斿緫鐨勫弽鎳�(y墨ng)閬庣▼锛氬弽鎳�(y墨ng)鐨勭涓€闅庢锛�6-纾烽吀钁¤悇绯栬劔姘叾鍙�
6-纾烽吀钁¤悇绯栭吀鑴矮閰�銆傜７閰告垔绯栭€斿緫鐨勮(di脿o)绡€(ji茅)锛�6-纾烽吀钁¤悇绯栬劔姘叾鏄棞(gu膩n)閸甸叾銆傜７閰告垔绯�
閫斿緫鐨勭敓鐞嗘剰缇�銆�
5. 绯栧師鐨勫悎鎴愯垏鍒嗚В锛氱硸鍘熺殑鍚堟垚浠ｈ瑵锛歎DPG 鏄椿鎬ц憽钀勭硸渚涢珨浠ュ強鍚堟垚閬庣▼銆傜硸鍘熺殑
鍒嗚В浠ｈ瑵锛氬垎瑙ｉ亷绋�銆傜硸鍘熷悎鎴愯垏鍒嗚В鐨勮(di脿o)绡€(ji茅)锛氱７閰稿寲閰�銆佺硸鍘熷悎閰剁殑鍏卞児(ji脿)淇＞瑾�(di脿o)绡€(ji茅)銆傜硸
鍘熺疮绌嶇櫏銆�
6. 绯栫暟鐢燂細绯栫暟鐢熼€斿緫锛氭蹇靛強绯栫暟鐢熺殑鍥涘€�(g猫)闂�(gu膩n)閸甸叾銆傜硸鐣扮敓鐨勮(di脿o)绡€(ji茅)銆傜硸鐣扮敓鐨勭敓鐞嗘剰缇┿€�
涔抽吀寰挵(hu谩n)锛氬惊鐠�(hu谩n)閬庣▼鍙婄敓鐞嗘剰缇�銆�
7. 琛€绯栧強鍏惰(di脿o)绡€(ji茅)锛氳绯栫殑渚嗘簮鍜屽幓璺�銆傝绯栨按骞崇殑瑾�(di脿o)绡€(ji茅)锛氳儼宄剁礌銆佽儼楂樿绯栫礌銆佺硸鐨唱(zh矛)婵€
绱犲強鑵庝笂鑵虹礌鍚勮嚜灏�(du矛)琛€绯栫殑褰遍熆銆傝绯栨按骞崇暟甯革細楂樿绯栧強绯栧翱鐥�锛屼綆琛€绯栥€�
绗簲绔� 鑴傞浠ｈ瑵
1.鑴傝唱(zh矛)鐨勭ó椤炲強鍔熻兘锛氬涓嶉＝鍜岃剛閰哥殑閲嶈琛嶇敓鐗┾€斺€斿墠鍒楄吅绱�銆佽鏍撳檨鐑峰強鐧戒笁鐑細鍓�
鍒楄吅绱�銆佽鏍撳檨鐑峰強鐧戒笁鐑殑鍖栧(xu茅)绲�(ji茅)妲�(g貌u)銆佸懡鍚�銆佸悎鎴愬強鐢熺悊鍔熻兘銆�
2. 鑴傝唱(zh矛)鐨勬秷鍖栧拰鍚告敹锛氳剛椤炴秷鍖栫殑涓昏鍫�(ch菐ng)鎵€锛岃喗姹侀吀楣�銆佽儼鑴傞叾銆佽紨鑴傞叾鐨勪綔鐢�锛岃剛鑲悎
鎴愮殑鐢樻补涓€閰€斿緫銆�
3. 鐢樻补涓夐叝浠ｈ瑵 锛氱敇娌逛笁閰殑鍚堟垚浠ｈ瑵 锛氬悎鎴愰儴浣�銆佸師鏂�銆佸悎鎴愬熀鏈亷绋嬶細鐢樻补涓€閰�
閫斿緫鍜岀敇娌逛簩閰€斿緫銆傜敇娌逛笁閰殑鍒嗚В浠ｈ瑵锛氳剛鑲殑鍕�(d貌ng)鍝★細婵€绱犳晱鎰熸€х敇娌逛笁閰剛鑲叾銆�
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绶氱矑楂�锛岃剛鑲吀鐨勎�-姘у寲锛岃剛鑲吀姘у寲鐨勮兘閲忕敓鎴�銆傞叜楂旂殑鐢熸垚鍙婂埄鐢細閰珨鐨勬蹇碉紝閰�
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CoA銆佽兘閲忓強渚涙矮鐗╄唱(zh矛)銆傚悎鎴愬熀鏈亷绋嬶細鑶藉浐閱囧悎鎴愮殑闄愰€熼叾銆佸悎鎴愮殑鍩烘湰閬庣▼銆傝喗鍥洪唶鍚�

 
 
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绗叚绔� 鐢熺墿姘у寲
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6锛庨€氶亷绶氱矑楂斿収(n猫i)鑶滅殑鐗╄唱(zh矛)杞�(zhu菐n)閬�(y霉n)锛氱窔绮掗珨鍏�(n猫i)鑶滅殑涓昏杞�(zhu菐n)閬�(y霉n)铔嬬櫧锛岃優婕夸腑 NADH 鐨勬哀鍖栵紞伪-纾�
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绗竷绔� 姘ㄥ熀閰镐唬璎�
1. 铔嬬櫧璩�(zh矛)鐨勭嚐(y铆ng)椁�(y菐ng)浣滅敤锛氳泲鐧借唱(zh矛)鐕�(y铆ng)椁�(y菐ng)鐨勯噸瑕佹€�銆傝泲鐧借唱(zh矛)鐨勯渶瑕侀噺鍜岀嚐(y铆ng)椁�(y菐ng)鍍�(ji脿)鍊硷細姘钩琛★紝鐢熺悊
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2. 蹇呴渶姘ㄥ熀閰哥殑姒傚康鍜岀ó椤炪€�
3. 铔嬬櫧璩�(zh矛)鐨勬秷鍖�銆佸惛鏀惰垏鑵�?銆傛棻闉嶈釜|(zh矛)鐨勬秷鍖栵細鑳冧腑鐨勬秷鍖栵紝灏忚吀涓殑娑堝寲銆傝泲鐧借唱(zh矛)鐨勮厫
鏁椾綔鐢細鑳洪鐨勭敓鎴�锛屾皑鐨勭敓鎴�锛屽叾浠栨湁瀹崇墿璩�(zh矛)鐨勭敓鎴�銆�
4. 姘ㄥ熀閰哥殑涓€鑸唬璎� 锛氶珨鍏�(n猫i)铔嬬櫧璩�(zh矛)鐨勮綁(zhu菐n)鎻涙洿鏂帮細楂斿収(n猫i)姘ㄥ熀閰哥殑闄嶈В鍙婃皑鍩洪吀鐨勪唬璎濆韩(k霉)鐨�
姒傚康銆傛皑鍩洪吀鐨勮劔姘ㄥ熀浣滅敤锛氳伅(li谩n)鍚堣劔姘ㄥ熀浣滅敤銆傝綁(zhu菐n)姘ㄥ熀浣滅敤锛氳綁(zhu菐n)姘ㄩ叾鑸囪綁(zhu菐n)姘ㄥ熀浣滅敤锛岃綁(zhu菐n)姘�
鍩轰綔鐢ㄧ殑姗�(j墨)鍒�銆侺-璋锋皑閰告哀鍖栬劔姘ㄥ熀浣滅敤銆傚槍鍛ゆ牳鑻烽吀寰挵(hu谩n)銆偽�-閰吀鐨勪唬璎� 锛氱稉(j墨ng)姘у寲鐢�
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5. 姘ㄧ殑浠ｈ瑵: 楂斿収(n猫i)姘ㄧ殑渚嗘簮锛氭皑鍩洪吀鑴皑鍩轰綔鐢ㄧ敘(ch菐n)鐢熺殑姘ㄦ槸楂斿収(n猫i)姘ㄧ殑涓昏渚嗘簮锛岃吀閬撳惛鏀�
鐨勬皑锛岃厧灏忕涓婄毊绱�(x矛)鑳炲垎娉岀殑姘ㄤ富瑕佷締鑷胺姘ㄩ叞鑳�銆傛皑鐨勮綁(zhu菐n)閬�(y霉n)锛氫笝姘ㄩ吀-钁¤悇绯栧惊鐠�(hu谩n)锛岃胺姘�
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槌ユ皑閰稿惊鐠�(hu谩n)鐨勮┏绱�(x矛)姝ラ锛屽翱绱犲悎鎴愮殑瑾�(di脿o)绡€(ji茅)銆傞珮姘ㄨ鐧ュ拰姘ㄤ腑姣�銆�
6. 鍊�(g猫)鍒ユ皑鍩洪吀鐨勪唬璎濓細姘ㄥ熀閰哥殑鑴晶鍩轰綔鐢細纬-姘ㄥ熀涓侀吀 銆�?锛涙挰甯封挃M鑳�銆�5-缇ヨ壊鑳�銆�
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杞�(zhu菐n)璁�锛屼竴纰冲柈浣嶇殑鐢熺悊鍔熻兘銆傚惈纭皑鍩洪吀浠ｈ瑵 锛氱敳纭皑閰哥殑浠ｈ瑵锛氱敳纭皑閰歌垏杞�(zhu菐n)鐢插熀浣�
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绱犵殑鍚堟垚锛岄叒姘ㄩ吀鐨勫垎瑙ｄ唬璎濓紝鑻笝閰吀灏跨櫏銆傝壊姘ㄩ吀鐨勪唬璎�銆�
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2.鍢屽懁鍜屽槯鍟舵牳鑻烽吀瑁�(b菙)鏁戝悎鎴�銆�
3.鑴哀鏍歌嫹閰哥殑鐢熸垚銆�
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尾-涓欐皑閰�銆佄�-姘ㄥ熀鐣颁竵閰�銆�
5.鎶楁牳鑻烽吀浠ｈ瑵钘ョ墿鐨勭敓鍖栨(j墨)鍒�銆�
绗節绔� 闈炵嚐(y铆ng)椁�(y菐ng)鐗╄唱(zh矛)浠ｈ瑵
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2. 琛€绱呰壊鐨勭敓鐗╁悎鎴愶細鍚堟垚鍘熸枡锛岄檺閫熼叾锛屽悎鎴愰亷绋�銆傝绱呰壊鐨勭敓鐗╁悎鎴愮殑瑾�(di脿o)绡€(ji茅)銆�
3. 鑶芥眮鑸囪喗姹侀吀鐨勪唬璎濓細鑶芥眮銆傝喗姹侀吀鐨勫垎椤烇細娓搁洟鑶芥眮閰�銆佺祼(ji茅)鍚堣喗姹侀吀銆佸垵绱�(j铆)鑶芥眮閰稿拰
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4. 鑶借壊绱犵殑浠ｈ瑵鑸囬粌鐤革細鑶界磪绱犵殑鐢熸垚鍜岃綁(zhu菐n)閬�(y霉n)銆傝喗绱呯礌鍦ㄨ倽涓殑杞�(zhu菐n)璁�銆傝喗绱呯礌鍦ㄨ吀閬撲腑鐨�
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3锛嶥NA 鐢熺墿鍚堟垚閬庣▼锛氬師鏍哥敓鐗╃殑 DNA 鐢熺墿鍚堟垚锛氳捣濮嬶紙瑙ｉ張銆佸紩鐧�(f膩)楂斿拰寮曠墿锛�锛屽欢闀�(zh菐ng)锛�
绲傛銆傜湡鏍哥敓鐗╃殑 DNA 鐢熺墿鍚堟垚锛氱窗(x矛)鑳炲懆鏈�銆傚悎鎴愰亷绋嬶細璧峰锛屽欢闀�(zh菐ng)锛岀祩姝€€傜绮掗叾銆�
4. 閫嗚綁(zhu菐n)閷勫拰鍏朵粬寰�(f霉)鍒舵柟寮忥細閫嗚綁(zhu菐n)閷勭梾姣掑拰閫嗚綁(zhu菐n)閷勯叾銆傞€嗚綁(zhu菐n)閷勭爺绌剁殑鎰忕京銆�
5. DNA 鎼嶅偡锛堢獊璁婏級鑸囦慨寰�(f霉)锛氱獊璁婄殑鎰忕京銆傚紩鐧�(f膩)绐佽畩鐨勫洜绱�銆傜獊璁婄殑鍒嗗瓙鏀硅畩椤炲瀷锛氶尟(cu貌)閰嶃€�
缂哄け銆佹彃鍏�銆佹绉荤獊璁娿€侀噸鎺�銆侱NA 鎼嶅偡鐨勪慨寰�(f霉)锛氬厜淇京(f霉)銆佸垏闄や慨寰�(f霉)銆侀噸绲勪慨寰�(f霉)銆丼OS 淇京(f霉)銆�
绗崄涓€绔� RNA 鐨勭敓鐗╁悎鎴愶紙杞�(zhu菐n)閷勶級
1. 寰�(f霉)鍒惰垏杞�(zhu菐n)閷勭殑鍗€(q奴)鍒ャ€�
2. 杞�(zhu菐n)閷勭殑妯℃澘鍜岄叾锛氳綁(zhu菐n)閷勬ā鏉匡細绲�(ji茅)妲�(g貌u)鍩哄洜锛屼笉灏�(du矛)绋辫綁(zhu菐n)閷勶紝妯℃澘閺�锛岀法纰奸張銆� RNA 鑱氬悎閰讹細
鍘熸牳鐢熺墿鐨� RNA 鑱氬悎閰讹紙鏍稿績閰�銆佸叏閰讹級锛岀湡鏍哥敓鐗╃殑 RNA 鑱氬悎閰讹紙鈪�銆佲叀銆佲參锛�銆傛ā鏉�
鑸囬叾鐨勮鲸瑾�(r猫n)绲�(ji茅)鍚堛€�

 
 
3. 杞�(zhu菐n)閷勯亷绋嬶細鍘熸牳鐢熺墿杞�(zhu菐n)閷勯亷绋嬶細杞�(zhu菐n)閷勮捣濮�銆佸欢闀�(zh菐ng)锛堣綁(zhu菐n)閷勭┖娉★級銆佺祩姝紙渚濊炒 Rho銆侀潪渚�
璩� Rho 鐨勮綁(zhu菐n)閷勭祩姝級銆傜湡鏍哥敓鐗╄綁(zhu菐n)閷勯亷绋嬶細杞�(zhu菐n)閷勮捣濮嬶紙TATA 鐩掓垨 Hogness 鐩掞紝杞�(zhu菐n)閷勫洜瀛�锛�
杞�(zhu菐n)閷勮捣濮嬪墠寰�(f霉)鍚堢墿锛�銆佸欢闀�(zh菐ng)銆佺祩姝紙鐪熸牳鐢熺墿杞�(zhu菐n)閷勭祩姝㈢殑淇＞榛�(di菐n)锛�銆�
4. 鐪熸牳鐢熺墿鐨勮綁(zhu菐n)閷勫悗淇＞锛氱湡鏍哥敓鐗� mRNA 鐨勮綁(zhu菐n)閷勫悗鍔犲伐锛氶銆佸熬鐨勪慨椋�锛宮RNA 鐨勫壀鎺ャ€�
tRNA 鐨勮綁(zhu菐n)閷勫悗鍔犲伐锛�5鈥欏墠灏�(d菐o)搴忓垪鍒囬櫎锛岀█鏈夊牽鍩虹敓鎴愶紙鐢插熀鍖栥€侀倓鍘熴€佹牳鑻峰収(n猫i)鐨勮綁(zhu菐n)浣�銆�
鑴皑锛夛紝3鈥欐湯绔姞 CCA锛峅H銆俽RNA 鐨勮綁(zhu菐n)閷勫悗鍔犲伐锛�45S RNA 鍓帴銆傛牳閰讹細鏍搁叾鐨勭壒鎬э紙鏍�
閰朵綔鐢ㄧ殑鍩虹(ch菙)鈥旈寴闋祼(ji茅)妲�(g貌u)锛�锛屾牳閰剁爺绌剁殑鎰忕京銆�
绗崄浜岀珷 铔嬬櫧璩�(zh矛)鐨勭敓鐗╁悎鎴愶紙缈昏锛�
1. 铔嬬櫧璩�(zh矛)鐢熺墿鍚堟垚楂旂郴锛氱炕璀ā鏉� mRNA 鍙婇伜鍌冲瘑纰硷細閬哄偝瀵嗙⒓鐨勬蹇点€佺ó椤�銆佺壒榛�(di菐n)锛堟柟
鍚戞€с€侀€ｇ簩(x霉)鎬�銆佺啊(ji菐n)骞舵€с€佹摵鍕�(d貌ng)鎬�銆侀€氱敤鎬э級銆傛牳铔嬬櫧楂旀槸澶氳偨閺堝悎鎴愮殑瑁濈疆銆倀RNA 鍔熻兘銆�
tRNA 姘ㄥ熀閰哥殑娲诲寲锛氭皑鍩洪叞-tRNA 鍚堟垚閰�锛岃捣濮嬫皑鍩洪叞-tRNA銆�
2. 铔嬬櫧璩�(zh矛)鐢熺墿鍚堟垚閬庣▼锛氳偨閺堝悎鎴愯捣濮嬶細鍘熸牳缈昏璧峰寰�(f霉)鍚堢墿褰㈡垚锛堟牳铔嬬櫧楂斾簽鍩哄垎闆�锛�
mRNA 灏忎簽鍩哄畾浣嶇祼(ji茅)鍚堬紝璧峰姘ㄥ熀閰�-tRNA 鐨勭祼(ji茅)鍚�锛屾牳铔嬬櫧楂斿ぇ浜炲熀绲�(ji茅)鍚堬級锛涚湡鏍哥敓鐗╃炕璀�
璧峰寰�(f霉)鍚堢墿褰㈡垚锛堟牳铔嬬櫧楂斿ぇ灏忎簽鍩哄垎闆�锛岃捣濮嬫皑鍩洪叞-tRNA 鐨勭祼(ji茅)鍚�锛宮RNA 鍦ㄦ牳铔嬬櫧楂斿皬
浜炲熀鐨勬簴(zh菙n)纰哄氨浣�锛屾牳铔嬬櫧楂斿ぇ浜炲熀绲�(ji茅)鍚堬級銆傝偨閺堢殑寤堕暦(zh菐ng)锛氭牳铔嬬櫧楂斿惊鐠�(hu谩n)锛堥€�(j矛n)浣�銆佹垚鑲�銆佽綁(zhu菐n)
浣嶏級銆傝偨閺堝悎鎴愮殑绲傛锛氳泲鐧借唱(zh矛)鐢熺墿鍚堟垚閬庣▼涓殑鑳介噺娑堣€楋紝澶氳仛鏍歌泲鐧介珨銆�
3. 铔嬬櫧璩�(zh矛)鍚堟垚鍚庡姞宸ュ拰杓搁€侊細澶氳偨閺堟姌鐤婄偤澶╃劧鍔熻兘妲�(g貌u)璞＄殑铔嬬櫧璩�(zh矛)锛氬垎瀛愪即渚讹紙鐔变紤鍏嬭泲
鐧�锛屼即渚剁礌锛�锛岃泲鐧戒簩纭嵉鐣版(g貌u)閰讹紝鑲斤紞鑴皑閰伴爢鍙嶇暟妲�(g貌u)閰�銆備竴绱�(j铆)绲�(ji茅)妲�(g貌u)鐨勪慨椋撅細鑲介張 N 绔�
鐨勪慨椋�锛屽€�(g猫)鍒ユ皑鍩洪吀鐨勫叡鍍�(ji脿)淇＞锛屽鑲介張鐨勬按瑙ｄ慨椋�銆傜┖闁撶祼(ji茅)妲�(g貌u)鐨勪慨椋撅細浜炲熀鑱氬悎锛岃紨鍩�
閫ｆ帴锛岀枏姘磋剛閺堢殑鍏卞児(ji脿)淇＞銆傝泲鐧借唱(zh矛)鍚堟垚鍚庣殑闈跺悜杓搁€侊細鍒嗘硨鎬ц泲鐧界殑闈跺悜杓搁€侊紙淇¤櫉(h脿o)鑲�锛�
淇¤櫉(h脿o)鑲借瓨(sh铆)鍒ラ绮�锛孲RP 灏�(du矛)鎺ヨ泲鐧斤級锛岀窔绮掗珨铔嬬櫧鐨勯澏鍚戣几閫�锛岀窗(x矛)鑳炴牳铔嬬櫧鐨勯澏鍚戣几閫侊紙鏍�
瀹氫綅搴忓垪锛�銆�
绗崄涓夌珷 鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺�
1. 鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺у熀鏈蹇佃垏鐗归粸(di菐n)锛氬熀鍥犺〃閬�(d谩)鐨勬蹇碉細鍩哄洜锛屽熀鍥犵祫锛屽熀鍥犺〃閬�(d谩)銆傚熀鍥犺〃閬�(d谩)
鐨勭壒鐣版€э細鏅�(sh铆)闁撴€у強绌洪枔鎬�?锛涱媰铏_(d谩)鐨勬柟寮忥細鍩烘湰琛ㄩ仈(d谩)锛堢祫鎴愭€ц〃閬�(d谩)锛�锛岃獦灏�(d菐o)鍜岄樆閬忚〃
閬�(d谩)?锛涱媰铏_(d谩)瑾�(di脿o)鎺х殑澶氬堡娆℃€у拰寰�(f霉)闆滄€�銆� 鍩哄洜琛ㄩ仈(d谩)鍙楅爢寮忎綔鐢ㄥ厓浠跺拰鍙嶅紡浣滅敤鍥犲瓙鍏卞悓瑾�(di脿o)
绡€(ji茅)銆傚熀鍥犺〃閬�(d谩)瑾�(di脿o)鎺х殑鐢熺墿瀛�(xu茅)鎰忕京銆�
2. 鍘熸牳鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺э細鍘熸牳鐢熺墿鍩哄洜绲勭祼(ji茅)妲�(g貌u)鐗归粸(di菐n)銆傚師鏍哥敓鐗╄綁(zhu菐n)閷勮(di脿o)鎺х殑鍩烘湰鍠綅-鎿嶇副瀛�
姒傚康銆備钩绯栨搷绺卞瓙鐨勭祼(ji茅)妲�(g貌u)锛圸銆乊 鍙� A 鍩哄洜锛屾搷绺卞簭鍒楋紝鍟熷嫊(d貌ng)搴忓垪锛岃(di脿o)绡€(ji茅)鍩哄洜锛孋AP 绲�(ji茅)鍚堜綅
榛�(di菐n)锛�锛屼钩绯栨搷绺卞瓙瑾�(di脿o)绡€(ji茅)姗�(j墨)鍒讹紙闃婚亸铔嬬櫧鐨勮矤(f霉)鎬ц(di脿o)绡€(ji茅)锛孋AP 鐨勬鎬ц(di脿o)绡€(ji茅)锛屽崝(xi茅)瑾�(di脿o)瑾�(di脿o)绡€(ji茅)锛�銆�
3. 鐪熸牳鍩哄洜杞�(zhu菐n)閷勮(di脿o)绡€(ji茅)锛氱湡鏍稿熀鍥犵祫绲�(ji茅)妲�(g貌u)鐗归粸(di菐n)锛氱湡鏍稿熀鍥犵祫绲�(ji茅)妲�(g貌u)榫愬ぇ锛屽柈闋嗗弽瀛�锛岄噸寰�(f霉)搴忓垪锛�
鍩哄洜涓嶉€ｇ簩(x霉)鎬с€俁NA pol 鈪¤綁(zhu菐n)閷勮捣濮嬬殑瑾�(di脿o)绡€(ji茅)锛氶爢寮忎綔鐢ㄥ厓浠讹紙鍟熷嫊(d貌ng)瀛愶紝澧炲挤(qi谩ng)瀛�锛屾矇榛樺瓙锛夛紝
鍙嶅紡浣滅敤鍥犲瓙锛堣綁(zhu菐n)閷勫洜瀛愮殑鍒嗛銆佺祼(ji茅)妲�(g貌u)锛�锛宮RNA 杞�(zhu菐n)閷勬縺娲诲強鍏惰(di脿o)绡€(ji茅)锛圱F 鈪 绲勬垚鎴愬垎鈥斺€�
TBP銆乀AF锛�銆�

 
 
绗崄鍥涚珷 绱�(x矛)鑳炰俊铏�(h脿o)杞�(zhu菐n)灏�(d菐o)鐨勫垎瀛愭(j墨)鍒�
1.绱�(x矛)鑳炰俊鎭墿璩�(zh矛)鐨勬蹇靛強鍒嗛銆�
2.鍙楅珨鐨勬蹇�銆佸垎椤炲拰浣滅敤鐗归粸(di菐n)銆�
3.G 铔嬬櫧銆�
4.鑶滃彈楂斾粙灏�(d菐o)鐨勪俊铏�(h脿o)杞�(zhu菐n)灏�(d菐o)姗�(j墨)鍒讹細cAMP-铔嬬櫧婵€閰堕€斿緫銆�
5.鑳炲収(n猫i)鍙楅珨浠嬪皫(d菐o)鐨勪俊铏�(h脿o)杞�(zhu菐n)灏�(d菐o)姗�(j墨)鍒讹細鐢茬媭鑵虹礌銆侀鍥洪唶婵€绱犵殑瑾�(di脿o)绡€(ji茅)閬庣▼銆�
绗崄浜旂珷 DNA 閲嶇祫鍙婇噸绲� DNA 鎶€琛�(sh霉)
1.鑷劧鐣� DNA 鐨勯噸绲勫拰鍩哄洜杞�(zhu菐n)绉讳富瑕佹柟寮忥細鍚屾簮閲嶇祫銆傜窗(x矛)鑿岀殑鍩哄洜杞�(zhu菐n)绉昏垏閲嶇祫锛氭帴鍚堜綔鐢�锛�
杞�(zhu菐n)鍖栦綔鐢�锛岃綁(zhu菐n)灏�(d菐o)浣滅敤銆傜壒鐣颁綅榛�(di菐n)閲嶇祫锛毼诲櫖鑿岄珨 DNA 鐨勬暣鍚堬紝绱�(x矛)鑿岀殑鐗圭暟浣嶉粸(di菐n)閲嶇祫銆傝綁(zhu菐n)搴�
閲嶇祫銆�
2. 閲嶇祫 DNA 鎶€琛�(sh霉)鐩搁棞(gu膩n)姒傚康锛氶噸绲� DNA锛圖NA 鍏嬮殕銆佸熀鍥犲厠闅嗭級锛岄檺鍒舵€ф牳閰稿収(n猫i)鍒囬叾锛岀洰鐨�
鍩哄洜锛屽熀鍥犺級楂旓紙璩�(zh矛)绮�銆佸櫖鑿岄珨锛�銆�
3锛庨噸绲� DNA 鎶€琛�(sh霉)鍩烘湰鍘熺悊鍙婃搷浣滄椹燂細鐩殑鍩哄洜鐨勭嵅?銆傚く鐦淲(xu茅)鍚堟垚娉�锛屽熀鍥犵祫 DNA 鏂囧韩(k霉)锛�
cDNA 鏂囧韩(k霉)锛岃仛鍚堥叾閺堝弽鎳�(y墨ng)銆傚厠闅嗚級楂旂殑閬告搰鍜屾(g貌u)寤�銆傚婧愬熀鍥犺垏杓夐珨鐨勯€ｆ帴銆傞噸绲� DNA 灏�(d菐o)
鍏ュ彈楂旇弻锛氭劅鍙楁厠(t脿i)绱�(x矛)鑳�锛屾柟寮忊€旇綁(zhu菐n)鍖栥€佽綁(zhu菐n)鏌撳拰鎰熸煋銆傞噸绲勯珨鐨勭閬搞€�
鍏嶇柅瀛�(xu茅)
绗竴绔� 鍏嶇柅瀛�(xu茅)姒傝堪鍜岀櫦(f膩)灞曞彶
1.鍏嶇柅鐨勬蹇�锛屽厤鐤郴绲�(t菕ng)鍙婂叾绲勬垚锛屽厤鐤郴绲�(t菕ng)鐨勪笁澶у姛鑳�銆�
2.涓鑸囧鍛ㄥ厤鐤櫒瀹樼殑绋銆佺祫鎴愬拰涓昏鍏嶇柅鍔熻兘銆�
3.娣嬪反绱�(x矛)鑳炲啀寰挵(hu谩n)鐨勫厤鐤(xu茅)鎰忕京銆�
4. 鍏嬮殕閬告搰瀛�(xu茅)瑾�銆�
绗簩绔� 鎶楀師
1.鎶楀師銆佸崐鎶楀師銆佸厤鐤師銆佹姉鍘熻〃浣嶃€佽秴鎶楀師銆佷綈鍔戠殑姒傚康锛屾姉鍘熺殑鍩烘湰鎬ц唱(zh矛)锛屾姉鍘熺殑鐗�
鎬э紝TD-Ag 鍜� TI-Ag 鐨勫崁(q奴)鍒�銆�
2.琛ㄤ綅姒傚康鑸囩ó椤�锛孴 绱�(x矛)鑳炶〃浣嶅拰 B 绱�(x矛)鑳炶〃浣嶇殑鍗€(q奴)鍒�銆�
3.姹哄畾鎶楀師鍏嶇柅鍘熸€у拰鐗圭暟鎬х殑鍥犵礌锛氭姉鍘熷垎瀛愮殑鐞嗗寲鐗规€�銆佸涓绘柟闈㈢殑鍥犵礌銆侀€�(j矛n)鍏ユ(j墨)楂�
鐨勯€斿緫銆�
4.浜ゅ弶鍙嶆噳(y墨ng)鍙婂叾鐢熺墿瀛�(xu茅)鎰忕京銆�
5.鍚勭ó椤炴姉鍘熷拰闈炵壒鐣版€у厤鐤埡婵€鍔戝強鍏堕啱(y墨)瀛�(xu茅)鎰忕京銆�
6.甯哥敤鐨勬湁绲插垎瑁傚師銆佸紬姘忎綈鍔戜綔鐢ㄦ(j墨)鍒�銆�
绗笁绔� 鍏嶇柅鐞冭泲鐧�
1.鍏嶇柅鐞冭泲鐧界殑姒傚康銆侀鍨�銆佸熀鏈祼(ji茅)妲�(g貌u)銆�
2.鍏嶇柅鐞冭泲鐧界殑鍔熻兘鍗€(q奴)鍙婂悇鍔熻兘鍗€(q奴)鐨勫姛鑳�銆�

 
 
3.鍚勯鍏嶇柅鐞冭泲鐧界殑鐢熺墿瀛�(xu茅)鐗规€у強鍔熻兘銆�
4.鍏嶇柅鐞冭泲鐧界殑閰惰瑙ｇ墖娈�銆�
5.澶氬厠闅嗘姉楂斻€佸柈鍏嬮殕鎶楅珨鍙婂悇绋熀鍥犲伐绋嬫姉楂旂殑姒傚康銆佺壒榛�(di菐n)鍜屾噳(y墨ng)鐢ㄣ€�
绗洓绔� 瑁�(b菙)楂旂郴绲�(t菕ng)
1.瑁�(b菙)楂旂殑姒傚康銆佺祫鎴�銆佺敘(ch菐n)鐢熼儴浣嶃€佺悊鍖栫壒鎬�銆�
2.瑁�(b菙)楂斾笁姊濇縺娲婚€斿緫锛氱稉(j墨ng)鍏告縺娲婚€斿緫銆佹梺璺縺娲婚€斿緫銆佺敇闇茬硸绲�(ji茅)鍚堝嚌闆嗙礌锛圡BL锛夋縺娲婚€斿緫
鐨勭暟鍚岄粸(di菐n)銆�
3.瑁�(b菙)楂旀椿鍖栫殑瑾�(di脿o)绡€(ji茅)姗�(j墨)鍒�銆�
4.瑁�(b菙)楂旂郴绲�(t菕ng)鐨勭敓鐗╁(xu茅)娲绘€�銆�
绗簲绔� 绱�(x矛)鑳炲洜瀛�
1.绱�(x矛)鑳炲洜瀛愮殑鍩烘湰姒傚康銆佸垎椤炲拰浣滅敤鐗归粸(di菐n)銆�
2.绱�(x矛)鑳炲洜瀛愪富瑕佺殑鐢熺墿瀛�(xu茅)娲绘€�銆�
绗叚绔� 鐧界窗(x矛)鑳炲垎鍖栨姉鍘熷拰绮橀檮鍒嗗瓙
1.鐧界窗(x矛)鑳炲垎鍖栨姉鍘熴€丆D 鎶楀師銆佺窗(x矛)鑳炵矘闄勫垎瀛愮殑姒傚康銆�
2.鑸� T 绱�(x矛)鑳炶瓨(sh铆)鍒�銆佹椿鍖栧拰鏁堟噳(y墨ng)鐩搁棞(gu膩n)鐨� CD 鍒嗗瓙銆�
3.鑸� B 绱�(x矛)鑳炶瓨(sh铆)鍒�銆佹椿鍖栧拰鏁堟噳(y墨ng)鐩搁棞(gu膩n)鐨� CD 鍒嗗瓙銆�
4.绱�(x矛)鑳炵矘闄勫垎瀛愮殑鍒嗛銆佺壒鎬у拰鍔熻兘銆�
绗竷绔� 涓昏绲勭箶鐩稿鎬у熀鍥犲京(f霉)鍚堥珨鑸囧叾绶ㄧ⒓鐨勫垎瀛�
1.MHC銆丠LA 鐨勬蹇�銆�
2.HLA 鍩哄洜寰�(f霉)鍚堥珨鐨勫熀鏈祼(ji茅)妲�(g貌u)锛孧HC 澶氭厠(t脿i)鎬ф蹇点€�
3.MHC I 椤炲拰 II 椤炲垎瀛愮殑绲�(ji茅)妲�(g貌u)銆佺祫绻旂窗(x矛)鑳炲垎甯冨拰鑸囨姉鍘熻偨鐩镐簰浣滅敤鐗归粸(di菐n)銆�
4.MHC I 椤炲拰 II 椤炲垎瀛愬湪铔嬬櫧璩�(zh矛)鎶楀師铏曠悊鍜屾彁鍛堜腑鐨勪綔鐢�銆�
5.MHC 閬哄偝瀛�(xu茅)鐗瑰緛銆�
绗叓绔� 鍏嶇柅绱�(x矛)鑳�
1. T 绱�(x矛)鑳�銆丅 绱�(x矛)鑳炲湪璀�(sh铆)鍒ユ姉鍘熸柟闈㈢殑宸暟锛屽強鍏跺湪鎶楃梾鍘熼珨鎰熸煋鏂归潰鐨勪綔鐢ㄣ€�
2. T 绱�(x矛)鑳炲拰 B 绱�(x矛)鑳炵殑鍒嗗寲鐧�(f膩)鑲茶垏姗�(j墨)楂斿厤鐤嚜绌�(w臎n)鍔熻兘褰㈡垚鐨勯棞(gu膩n)绯�銆�
3. T 绱�(x矛)鑳炲拰 B 绱�(x矛)鑳炵殑琛ㄩ潰妯�(bi膩o)蹇楀強鍏朵綔鐢ㄣ€�
4. T 绱�(x矛)鑳炰簽缇ゅ強鍏跺姛鑳�銆�
5. B 绱�(x矛)鑳炰簽缇ゅ強鍏跺姛鑳�銆�
6. NK 绱�(x矛)鑳炴椿鍖栧拰鎶戝埗鍙楅珨鐨勪綔鐢ㄦ(j墨)鍒�銆�
绗節绔� 鍥烘湁鍏嶇柅鎳�(y墨ng)绛�
1. 澶╃劧鍏嶇柅鎳�(y墨ng)绛旂殑鍩烘湰姒傚康鍜岀壒榛�(di菐n)銆�
2. 鍚炲櫖绱�(x矛)鑳炶瓨(sh铆)鍒ュ井鐢熺墿鐨勬(j墨)鍒跺強鍏朵富瑕佺敓鐗╁(xu茅)浣滅敤銆�
3锛庡浐鏈夊厤鐤噳(y墨ng)绛旂殑浣滅敤鏅�(sh铆)鐩稿強鍏朵富瑕佷綔鐢ㄣ€�

 
 
4锛庡浐鏈夊厤鐤噳(y墨ng)绛斿拰閬╂噳(y墨ng)鎬у厤鐤噳(y墨ng)绛旂殑涓昏鐗归粸(di菐n)鍜岀浉浜掗棞(gu膩n)绯�
绗崄绔� 鎶楀師鎻愬憟绱�(x矛)鑳炲強鍏跺皪(du矛)鎶楀師鐨勮檿鐞嗗拰鎻愬憟
1. 鎶楀師鎻愬憟绱�(x矛)鑳炵殑姒傚康銆佺ó椤�銆�
2.鍏�(n猫i)婧愭€ф姉鍘熷拰澶栨簮鎬ф姉鍘熺殑鍗€(q奴)鍒ャ€�
3.DC 灏�(du矛)鍏�(n猫i)婧愭€ф姉鍘熷拰澶栨簮鎬ф姉鍘熺殑鎻愬憟閫斿緫锛屼氦鍙夋彁鍛�銆�
绗崄涓€绔� T 娣嬪反绱�(x矛)鑳炰粙灏�(d菐o)鐨勭窗(x矛)鑳炲厤鐤噳(y墨ng)绛�
1. T 绱�(x矛)鑳炶瓨(sh铆)鍒ユ姉鍘熺殑鐗归粸(di菐n)銆�
2. T 绱�(x矛)鑳炴椿鍖栫殑淇¤櫉(h脿o)瑕佹眰銆�
3. 鍏嶇柅绐佽Ц鐨勫舰鎴愬拰姒傚康
4. 鏁堟噳(y墨ng)鎬� CD4+ T 绱�(x矛)鑳炲拰 CD8+ T 绱�(x矛)鑳炵殑鍔熻兘
绗崄浜岀珷 B 绱�(x矛)鑳炰粙灏�(d菐o)鐨勯珨娑插厤鐤噳(y墨ng)绛�
1. B 绱�(x矛)鑳炲皪(du矛) TD-Ag銆乀I-Ag 鎶楀師鍏嶇柅鎳�(y墨ng)绛旂殑鐣板悓榛�(di菐n)銆�
2. Th 绱�(x矛)鑳炲湪 B 绱�(x矛)鑳炵殑鍏嶇柅鎳�(y墨ng)绛斾腑鐨勮紨鍔╀綔鐢�銆�
3. 楂旀恫鍏嶇柅鎳�(y墨ng)绛旂殑涓€鑸(gu墨)寰�,鍙婂叾鍦ㄩ爯(y霉)闃�銆佽ê鏂蜂腑鐨勬剰缇┿€�
4. 绮樿啘鍏嶇柅鎳�(y墨ng)绛旂殑鐗归粸(di菐n)銆�
5. B 绱�(x矛)鑳炲湪鐢熺櫦(f膩)涓績鐨勫垎鍖栨垚鐔熼亷绋�銆�
绗崄涓夌珷 鍏嶇柅鑰愬彈
1.鍏嶇柅鑰愬彈鐨勫熀鏈蹇�銆�
2.鍏嶇柅鑰愬彈褰㈡垚鐨勪富瑕佹(j墨)鍒躲€�
3.寤虹珛鍜屾墦鐮村厤鐤€愬彈鐨勪富瑕佹柟娉�銆�
4.鍏嶇柅鑰愬彈鑸囪嚚搴婇啱(y墨)瀛�(xu茅)鐨勯棞(gu膩n)绯�銆�
绗崄鍥涚珷 鍏嶇柅瑾�(di脿o)绡€(ji茅)
1.Th1/Th2 浜炵兢鐨勫垎鍖栬(di脿o)绡€(ji茅)鍜岀窗(x矛)鑳炲洜瀛愮殑闂�(gu膩n)绯�銆�
2.婵€娲绘€у彈楂斿拰鎶戝埗鎬у彈楂旂殑瑾�(di脿o)绡€(ji茅)浣滅敤銆�
3.鐛�(d煤)鐗瑰瀷缍�(w菐ng)绲�(lu貌)鍜屾縺娲昏獦灏�(d菐o)鐨勭窗(x矛)鑳炴浜″湪鐗圭暟鎬у厤鐤噳(y墨ng)绛旇(di脿o)绡€(ji茅)涓殑浣滅敤銆�
绗崄浜旂珷 瓒呮晱鍙嶆噳(y墨ng)
1. I銆両I銆両II銆両V 鍨嬭秴鏁忓弽鎳�(y墨ng)鐧�(f膩)鐢熺殑姗�(j墨)鍒�銆�
2. 鍥涘瀷瓒呮晱鍙嶆噳(y墨ng)寮曡捣鐨勪富瑕佺柧鐥呭強鍏剁壒榛�(di菐n)銆�
绗崄鍏珷 鍏嶇柅瀛�(xu茅)鎳�(y墨ng)鐢�
1. 楂斿鎶楀師-鎶楅珨鍙嶆噳(y墨ng)鐨勭壒榛�(di菐n)銆佸奖闊垮洜绱�銆�
2. 鎶楀師鎶楅珨鍙嶆噳(y墨ng)鐨勫熀鏈娓�(c猫)鏂规硶銆�
3. T銆丅 绱�(x矛)鑳炴暩(sh霉)閲忚垏鍔熻兘妾㈡脯(c猫)鐨勪富瑕佹柟娉�銆�
4. 閰惰伅(li谩n)鍏嶇柅鍚搁檮瑭﹂(y脿n)銆�
5.浜哄伐涓诲嫊(d貌ng)鍏嶇柅銆佷汉宸ヨ鍕�(d貌ng)鍏嶇柅鐨勬蹇�銆佺壒榛�(di菐n)鍙婃噳(y墨ng)鐢ㄨ寖鍦嶏紱甯歌鐨勭敓鐗╁埗鍝佺ó椤炲強鐗归粸(di菐n)銆�

 
 
鐥呯悊瀛�(xu茅)
绗竴绔� 绶掕珫
1. 鐥呯悊瀛�(xu茅)鐨勫収(n猫i)瀹瑰拰浠诲嫏(w霉)銆�
2. 鐥呯悊瀛�(xu茅)鍦ㄩ啱(y墨)瀛�(xu茅)涓殑鍦颁綅銆�
3. 鐥呯悊瀛�(xu茅)鐨勭爺绌舵柟娉�
绗簩绔� 绱�(x矛)鑳炲拰绲勭箶鐨勯仼鎳�(y墨ng)鑸囨悕鍌�
1. 绱�(x矛)鑳炲拰绲勭箶骞剧ó甯歌鐨勯仼鎳�(y墨ng)鎬ц畩鍖栵紙钀庣府銆佽偉澶�銆佸鐢熷拰鍖栫敓锛夌殑姒傚康銆佸父瑕嬮鍨嬪強
鐥呯悊璁婂寲銆�
2. 绱�(x矛)鑳炲拰绲勭箶鎼嶅偡鐨勫師鍥犲拰姗�(j墨)鍒�銆�
3. 璁婃€х殑甯歌椤炲瀷锛氬寘鎷窗(x矛)鑳炴按鑵�銆佽剛鑲畩銆佺幓鐠冩ǎ璁�銆佹穩绮夋ǎ璁�銆佺矘娑叉ǎ璁娿€佺梾鐞嗘€�
鑹茬礌娌夎憲鍜岀梾鐞嗘€ч垼鍖栫殑姒傚康銆佺櫦(f膩)鐢熸(j墨)鍒跺拰鐥呯悊璁婂寲銆�
4. 澹炴鐨勬蹇点€佸熀鏈梾璁�銆侀鍨嬨€佺祼(ji茅)灞€鍜屽皪(du矛)姗�(j墨)楂旂殑褰遍熆銆�
5. 鍑嬩骸鐨勬蹇�銆佺櫦(f膩)鐢熸(j墨)鍒跺強褰㈡厠(t脿i)瀛�(xu茅)鐗瑰緛銆�
绗笁绔� 鎼嶅偡鐨勪慨寰�(f霉)
1. 鍐嶇敓鍜屼慨寰�(f霉)鐨勬蹇�锛屾牴鎿�(j霉)鍐嶇敓鑳藉姏浜洪珨绱�(x矛)鑳炵殑鍒嗛銆佸悇绋祫绻旂殑鍐嶇敓鑳藉姏銆侀亷绋嬪強褰�
闊垮啀鐢熺殑鍥犵礌銆�
2. 骞茬窗(x矛)鑳炵殑姒傚康鍜屽垎椤�銆�
3. 鑲夎娊绲勭箶鍜岀槩鐥曠祫绻旂殑姒傚康銆佸舰鎱�(t脿i)鐗瑰緛鍜屼綔鐢紝鍓�(chu脿ng)鍌锋剤鍚堢殑姒傚康銆佸熀鏈亷绋嬨€侀鍨嬪強
褰遍熆鍥犵礌銆�
绗洓绔� 灞€閮ㄨ娑插惊鐠�(hu谩n)闅滅
1. 鍏呰鍜屾筏琛€鐨勬蹇�銆侀鍨�銆佸師鍥犮€佺梾鐞嗚畩鍖栧強鍚庢灉銆�
2. 鍑鸿鐨勬蹇�銆佺梾鍥�銆佺梾鐞嗚畩鍖栧強鍚庢灉銆�
3. 琛€鏍撳舰鎴愬拰琛€鏍撶殑姒傚康锛岃鏍撳舰鎴愮殑姊濅欢銆佹(j墨)鍒跺拰閬庣▼锛岃鏍撶殑椤炲瀷銆佽鏍撶殑绲�(ji茅)灞€鍜�
灏�(du矛)姗�(j墨)楂旂殑褰遍熆銆�
4. 鏍撳鐨勬蹇碉紝鏍撳瓙鐨勯亱(y霉n)琛岄€斿緫銆侀鍨嬪拰灏�(du矛)姗�(j墨)楂旂殑褰遍熆銆�
5. 姊楁鐨勬蹇�銆佸師鍥�銆佹浠�銆佺梾鐞嗚畩鍖�銆侀鍨�銆佸皪(du矛)姗�(j墨)楂旂殑褰遍熆鍙婄祼(ji茅)灞€銆�
绗簲绔� 鐐庣櫏
1. 鐐庣櫏鐨勬蹇�銆佸師鍥犮€佸熀鏈梾鐞嗚畩鍖栵紙璁婅唱(zh矛)銆佹徊鍑哄強澧炵敓锛�銆佸眬閮ㄨ〃鐝�(xi脿n)鍜屽叏韬弽鎳�(y墨ng)鍙婄値
鐧ョ殑鍒嗛銆�
2. 鎬ユ€х値鐧ョ梾璁婇亷绋嬪強褰遍熆鍥犵礌銆佺値鐧ヤ粙璩�(zh矛)鍦ㄧ値鐧ラ亷绋嬩腑鐨勪綔鐢ㄥ強鍏舵剰缇┿€佹€ユ€х値鐧ョ殑
鐥呯悊瀛�(xu茅)椤炲瀷鍙婄祼(ji茅)灞€銆�
3. 鎱㈡€х値鐧ョ殑涓€鑸梾鐞嗚畩鍖栫壒榛�(di菐n)锛岃倝鑺借叓鎬х値鐨勬蹇�銆佸父瑕嬮鍨�銆佸舰鎴愭浠躲€佺祫鎴愭垚鍒�
鍙婂舰鎱�(t脿i)鐗归粸(di菐n)銆�
绗叚绔� 鑵槫

 
 
1. 鑵槫鐨勬蹇�銆佽叓鐦ゆ€у鐢熷拰闈炶叓鐦ゆ€у鐢熺殑鍗€(q奴)鍒�銆�
2. 鑵槫鐨勫ぇ楂斿拰绲勭箶褰㈡厠(t脿i)銆�
3. 鑵槫鐨勫垎鍖栧拰鐣板瀷鎬�銆�
4. 鑵槫鐨勫懡鍚嶅強鍒嗛鍘熷墖锛岀檶鍜岃倝鐦ょ殑姒傚康銆�
5. 鑵槫鐨勭敓闀�(zh菐ng)鏂瑰紡銆佺敓闀�(zh菐ng)鐗归粸(di菐n)鍜屾摯(ku貌)鏁ｉ€斿緫銆�
6. 鑵槫鐨勫垎绱�(j铆)鍜屽垎鏈�銆�
7. 鑵槫灏�(du矛)姗�(j墨)楂旂殑褰遍熆銆�
8. 鑹€ц叓鐦よ垏鎯℃€ц叓鐦ょ殑鍗€(q奴)鍒�銆�
9. 鐧屽拰鑲夌槫鐨勫崁(q奴)鍒�銆�
10. 甯歌鑵槫鐨勫ソ鐧�(f膩)閮ㄤ綅銆佸舰鎱�(t脿i)鐗归粸(di菐n)鍙婂皪(du矛)姗�(j墨)楂旂殑褰遍熆銆傚涔抽牠鐙€鐦ゃ€佽吅鐦�銆佸泭鑵虹槫銆侀睏鐧�銆�
鑵虹檶銆佺簴缍槫銆佽剛鑲槫銆佸钩婊戣倢鐦�銆佺簴缍倝鐦�銆佽剛鑲倝鐦�銆佸钩婊戣倢鑲夌槫銆侀鑲夌槫銆佺暩
鑳庣槫绛夛級銆�
11. 鐧屽墠鐥呰畩銆侀潪鍏稿瀷澧炵敓鍜屽師浣嶇檶鐨勬蹇靛強甯歌鐥呰畩鑸変緥銆�
12. 鑵槫鐧�(f膩)鐢熺殑鍒嗗瓙鐢熺墿瀛�(xu茅)鍩虹(ch菙)锛岃垏鐠�(hu谩n)澧冦€侀伜鍌冲強鍏嶇柅鐨勯棞(gu膩n)绯�銆�
绗竷绔� 蹇冭绠＄郴绲�(t菕ng)鐤剧梾
1. 鍕�(d貌ng)鑴堢播妯ｇ‖鍖栫櫏鐨勭梾鍥犮€佺櫦(f膩)鐥呮(j墨)鍒�銆佸熀鏈梾鐞嗚畩鍖�銆備富瑕�?ji菐ng)鐢▆鐨勭梾鐞嗚畩鍖栧強鍚庢灉銆傚啝
鐙€鍕�(d貌ng)鑴堢‖鍖栨€у績鑷熺梾鐨勯鍨嬪強鐥呯悊鏀硅畩锛屽績鑲屾姝荤殑鐧�(f膩)鐥呮(j墨)鍒�銆佺梾璁婂強绲�(ji茅)灞€鍜屽嵄瀹�銆�
2. 楂樿澹撶梾鐨勭梾鍥犲強鐧�(f膩)鐥呮(j墨)鍒�銆侀鍨�銆佺梾鐞嗚畩鍖�銆佸悇鏈熺殑鐥呯悊璁婂寲鍙婂悗鏋�锛屽悇鑷熷櫒鐨勭梾
璁婄壒榛�(di菐n)鍙婂嵄瀹虫€с€傞珮琛€澹撴€у績鑷熺梾鐨勭梾璁婄壒榛�(di菐n)鍜岃嚚搴婄梾鐞嗚伅(li谩n)绯�銆�
3. 棰�(f膿ng)婵曠梾鐨勭梾鍥犲拰鐧�(f膩)鐥呮(j墨)鍒躲€佸熀鏈梾璁�, 棰�(f膿ng)婵曟€у績鍏�(n猫i)鑶滅値銆佸績鑲岀値銆佸績鍖呯値銆侀棞(gu膩n)绡€(ji茅)鐐庡強
鐨啔鐥呰畩鐨勭梾鐞嗚畩鍖栥€佺祼(ji茅)灞€鍜屽嵄瀹�銆�
4. 鎰熸煋鎬у績鍏�(n猫i)鑶滅値鐨勭梾鍥�銆佺櫦(f膩)鐥呮(j墨)鍒躲€侀鍨�銆佺梾鐞嗚畩鍖栧強鑷ㄥ簥鐥呯悊鑱�(li谩n)绯�銆�
5. 蹇冪摚鑶滅梾鐨勭櫦(f膩)鐥呮(j墨)鍒躲€佺梾鐞嗗舰鎱�(t脿i)瀛�(xu茅)鐗归粸(di菐n)鍙婅嚚搴婄梾鐞嗚伅(li谩n)绯�銆�
绗叓绔� 鍛煎惛绯荤当(t菕ng)鐤剧梾
1. 澶ц憠鎬ц偤鐐�銆佸皬钁夋€ц偤鐐�銆佽粛鍦�(tu谩n)鑿屾€ц偤鐐�銆佺梾姣掓€ц偤鐐庡拰鏀師楂旀€ц偤鐐庣殑鐥呭洜銆佺櫦(f膩)鐥�
姗�(j墨)鐞嗗強鐥呯悊璁婂寲鍙婅嚚搴婄梾鐞嗚伅(li谩n)绯�銆傛瘮杓冨叾涓昏鐣板悓榛�(di菐n)銆�
2. 鎱㈡€ч樆濉炴€ц偤鐥�--鎱㈡€ф敮姘ｇ鐐庛€佹敮姘ｇ鍝枠銆佹敮姘ｇ鎿�(ku貌)寮电櫏銆佹參鎬ч樆濉炴€ц偤姘ｈ叓鍜�
鑲虹娌夎憲鐥呯殑鍘熷洜銆佺櫦(f膩)鐥呮(j墨)鐞嗐€佺梾鐞嗚畩鍖�銆佸悎骞剁櫏鍙婅嚚搴婄梾鐞嗚伅(li谩n)绯�銆�
3. 鑲哄績鐥呯殑鐥呭洜銆佺櫦(f膩)鐥呮(j墨)鍒跺拰鐥呯悊璁婂寲銆�
4. 鍛煎惛绐樿揩缍滃悎寰佺殑鐥呭洜銆佺櫦(f膩)鐥呮(j墨)鍒�銆佺梾鐞嗚畩鍖栧強鑷ㄥ簥鐥呯悊鑱�(li谩n)绯�銆�
5. 榧诲捊鐧屽強鑲虹檶鐨勭梾鍥�銆佸父瑕嬮鍨�銆佺梾鐞嗚畩鍖�銆佽綁(zhu菐n)绉婚€斿緫鍙婂叾闋�(y霉)鍚�銆�
绗節绔� 娑堝寲绯荤当(t菕ng)鐤剧梾
1. 鎱㈡€ф泛琛ㄦ€ц儍鐐庡拰鎱㈡€ц悗绺€ц儍鐐庣殑鐥呭洜鍜岀梾璁婄壒榛�(di菐n)銆�
2. 娑堝寲鎬ф桨鐦嶇梾鐨勭梾鍥�銆佺櫦(f膩)鐥呮(j墨)鍒躲€佸ソ鐧�(f膩)閮ㄤ綅銆佺梾鐞嗚畩鍖�銆佺祼(ji茅)灞€鍜屽苟鐧�(f膩)鐧ュ強鑷ㄥ簥鐥呯悊鑱�(li谩n)

 
 
绯�銆�
3. 闂屽熬鐐庡拰鐐庢€ц吀鐥呯殑鐥呭洜銆佺櫦(f膩)鐥呮(j墨)鍒�銆侀鍨�銆佺梾鐞嗚畩鍖栧強鍚堝苟鐧ャ€�
4. 鐥呮瘨鎬ц倽鐐庣殑鐥呭洜銆佸熀鏈梾鐞嗚畩鍖栥€佽嚚搴婄梾鐞嗛鍨�銆�
5. 涓夌ó甯歌鑲濈‖鍖栵紙闁€鑴堟€�銆佸姝诲悗鎬у強鑶芥眮鎬э級鐨勭梾鍥犲強鐧�(f膩)鐥呮(j墨)鍒躲€傝倽纭寲鐨勫熀鏈梾
鐞嗚畩鍖�锛屽悇鍨嬬殑鐥呰畩鐗归粸(di菐n)銆傞厭绮炬€ц倽鐥呯殑鐧�(f膩)鐥呮(j墨)鍒跺強鐥呰畩鐗归粸(di菐n)銆�
6. 娑堝寲绯荤当(t菕ng)甯歌鑵槫鐨勫ソ鐧�(f膩)閮ㄤ綅銆佺梾鐞嗚畩鍖栧拰杞�(zhu菐n)绉婚€斿緫銆傛棭鏈熺檶鑸囬€�(j矛n)灞曟湡鐧岀殑瀹氱京銆佽倝
鐪煎強绲勭箶瀛�(xu茅)椤炲瀷銆�
7. 鍘熺櫦(f膩)鎬ц倽鐧岀殑鐥呭洜鍜岀梾鐞嗚畩鍖�銆佽倝鐪煎拰绲勭箶瀛�(xu茅)椤炲瀷鍙婃摯(ku貌)鏁ｉ€斿緫銆�
绗崄绔� 娣嬪反閫犺绯荤当(t菕ng)鐤剧梾
1. 鍙嶆噳(y墨ng)鎬ф穻宸寸祼(ji茅)鐐庡父瑕嬪師鍥犲強鐥呯悊璁婂寲銆傚父瑕嬬壒鐣版€ф穻宸寸祼(ji茅)鐐庯紙绲�(ji茅)鏍搞€佽矒鎶撶梾銆佺祫绻旂窗(x矛)
鑳炲姝绘€ф穻宸寸祼(ji茅)鐐庯級鐨勭梾鍥�銆佺梾鐞嗘敼璁婂強閼掑垾瑷烘柗銆�
2. 娣嬪反鐦ょ殑姒傚康銆侀湇濂囬噾娣嬪反鐦ょ殑鍒嗗瀷銆佺梾鐞嗙壒榛�(di菐n)鍙婇爯(y霉)鍚庛€傞潪闇嶅閲戞穻宸寸槫鐨勫父瑕嬮鍨�銆�
鐥呰畩鐗归粸(di菐n)鍙婅嚚搴婄梾鐞嗚伅(li谩n)绯�銆�
3. 鐧借鐥呯殑鍩烘湰姒傚康銆佸垎椤�锛屾€ユ€х櫧琛€鐥呭拰鎱㈡€х櫧琛€鐥呯殑鐥呴噷鏀硅畩鍙婅嚚搴婄梾鐞嗚伅(li谩n)绯�銆�
绗崄涓€绔� 鍏嶇柅鎬х柧鐥�
1. 鑷韩鍏嶇柅鎬х柧鐥呯殑姒傚康銆佺梾鍥犲強鐧�(f膩)鐥呮(j墨)鍒躲€�
2. 甯歌鐨勮嚜韬厤鐤€х柧鐥�銆�
3. 鍣ㄥ畼鍜岄楂撶Щ妞嶆帓鏂ュ弽鎳�(y墨ng)鍙婃(j墨)鍒�銆佺梾鐞嗚畩鍖栥€�
4. 鑹炬粙鐥呯梾鍥�銆佹祦琛岀梾瀛�(xu茅)銆佺櫦(f膩)鐥呮(j墨)鍒跺強鐥呰畩鐗归粸(di菐n)銆�
绗崄浜岀珷 娉屽翱绯荤当(t菕ng)鐤剧梾
1. 鑵庡皬鐞冭厧鐐庣殑鍩烘湰姒傚康銆佺梾鍥犲強鐧�(f膩)鐥呮(j墨)鍒�銆佷富瑕佺梾鐞嗚畩鍖栧拰鐥呯悊鍒嗗瀷銆佽嚚搴婄梾鐞嗚伅(li谩n)绯汇€�
鑵庡皬鐞冭厧鐐庣殑鍩烘湰褰㈡厠(t脿i)璁婂寲鑸囪嚚搴婁富瑕佺櫏鐙€鐨勯棞(gu膩n)绯�銆�
2. 鎬ユ€ц厧灏忕悆鑵庣値鑸囨參鎬ц厧灏忕悆鑵庣値鐨勫崁(q奴)鍒ャ€�
3. 鑵庣泜鑵庣値鍜岄枔璩�(zh矛)鎬ц厧鐐庣殑鍩烘湰姒傚康銆佺梾鍥犵櫦(f膩)鐥呮(j墨)鍒�銆佺梾鐞嗚畩鍖栥€佸苟鐧�(f膩)鐧ュ強鑷ㄥ簥鐥呯悊鑱�(li谩n)
绯汇€�
4. 寮曡捣鑵庤嚐钀庣府鐨勫父瑕嬬柧鐥呭湪鐥呭洜銆佺櫦(f膩)鐥呮(j墨)鍒�銆佸舰鎱�(t脿i)瀛�(xu茅)璁婂寲鍙婂嵄瀹虫€ф柟闈㈢殑鍗€(q奴)鍒�銆�
5. 鑵庤嚐鍙婅唨鑳卞父瑕嬬殑鑵槫鐨勫舰鎱�(t脿i)瀛�(xu茅)鐗瑰緛銆佺梾鐞嗛鍨嬪強鑷ㄥ簥鐥呯悊鑱�(li谩n)绯�銆�
绗崄涓夌珷 鐢熸畺绯荤当(t菕ng)鐤剧梾
1. 瀛愬闋哥檶鐨勭梾鐞嗗舰鎱�(t脿i)鐗归粸(di菐n)鍜岃嚚搴婄梾鐞嗚伅(li谩n)绯�銆�
2. 钁¤悇鑳庡拰绲ㄦ瘺鑶滅殑涓婄毊鐧岀殑鐥呭洜鐧�(f膩)鐥�銆佺梾鐞嗗舰鎱�(t脿i)鐗归粸(di菐n)鍜岃嚚搴婄梾鐞嗚伅(li谩n)绯汇€�
3. 涔宠吅绾栫董鑵虹槫鍜屼钩鑵虹檶鐨勭梾鐞嗙壒榛�(di菐n)鍜岃嚚搴婄梾鐞嗚伅(li谩n)绯�銆�
绗崄鍥涚珷 鍏�(n猫i)鍒嗘硨绯荤当(t菕ng)鐤剧梾
1. 鍨傞珨鑵虹槫鐨勫垎椤炲強鍩烘湰鐥呰畩銆�
2. 闈炴瘨鎬х敳鐙€鑵鸿叓鐨勭梾鍥犲拰鐧�(f膩)鐥呮(j墨)鍒�銆佺梾璁婄櫦(f膩)灞曢亷绋嬪強鐥呰畩鐗归粸(di菐n)銆�

 
 
3. 姣掓€х敳鐙€鑵鸿叓鐨勭梾鍥犲強鐧�(f膩)鐥呮(j墨)鍒躲€佺梾鐞嗚畩鍖栧強鑷ㄥ簥鐥呯悊鑱�(li谩n)绯�銆�
4. 鐢茬媭鑵虹値鐨勫垎椤�銆佷簽鎬ユ€у拰鎱㈡€х敳鐙€鑵虹値鐨勭梾璁婄壒榛�(di菐n)銆�
5. 鐢茬媭鑵鸿叓鐦ょ殑鍒嗗瀷鍜岀梾璁婄壒榛�(di菐n)銆佸垎鍨嬪強闋�(y霉)鍚�銆�
绗崄浜旂珷 绁炵稉(j墨ng)绯荤当(t菕ng)鐤剧梾
1. 绁炵稉(j墨ng)绯荤当(t菕ng)鐨勫熀鏈梾璁娿€�
2. 娴佽鎬ц叇鑴婇珦鑶滅値鐨勫師鍥�銆佺梾鐞嗚畩鍖栧強鑷ㄥ簥鐥呯悊鑱�(li谩n)绯�銆傛毚鐧�(f膩)鎬ф祦鑵︾殑鐥呰畩鐗归粸(di菐n)銆�
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绗崄涓冪珷 瀵勭敓锜茬梾
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