閯窞澶у(xu茅)纰╁＋鐮旂┒鐢熷叆瀛�(xu茅)鑰冭│澶х侗

鐢熺墿鍖栧(xu茅)锛堜笁锛夎€冭│澶х侗
1銆佽€冭│鑼冨湇
鐢熺墿鍖栧(xu茅)鐨勫熀绀�(ch菙)鐭ヨ瓨鍜屽熀鏈悊璜栥€�
2銆佺瓟椤屾柟寮忚垏鏅傞枔
闁夊嵎銆佺瓎瑭�銆�180 鍒嗛悩
3銆佽│椤屽垎鍊硷細150 鍒�
4銆侀鍨嬭垏鍒嗗€煎垎甯冿細
鍚嶈瑙ｉ噵
绨＄瓟椤�
8 椤岋紝5 鍒�/椤�锛屽叡 40 鍒�
7 椤�锛�10 鍒�/椤�锛屽叡 70 鍒�
2 椤�锛�20 鍒�/椤�锛屽叡 40 鍒�
鍟忕瓟椤�
5銆佽€冩煡鑼冨湇锛�
绗竴绔� 铔嬬櫧璩�(zh矛)鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳�
1. 铔嬬櫧璩�(zh矛)鐨勫垎瀛愮祫鎴�: 姘ㄥ熀閰革細L-伪-姘ㄥ熀閰哥祼(ji茅)妲�(g貌u)閫氬紡鍜屽垎椤�銆�20 绋皑鍩洪吀鐨勮嫳鏂囧悕瑭炲強
绺绗﹁櫉銆佹皑鍩洪吀鐨勭悊鍖栨€ц唱(zh矛)銆傝偨锛氳偨閸佃垏鑲介張锛岃偨鑸囪泲鐧借唱(zh矛)鐨勫崁(q奴)鍒�锛岀敓鐗╂椿鎬ц偨銆�
2. 铔嬬櫧璩�(zh矛)鐨勫垎瀛愮祼(ji茅)妲�(g貌u): 铔嬬櫧璩�(zh矛)鐨勪竴绱氱祼(ji茅)妲�(g貌u)锛氱董鎸佷竴绱氱祼(ji茅)妲�(g貌u)绌�(w臎n)瀹氱殑鍖栧(xu茅)閸碉紱铔嬬櫧璩�(zh矛)鐨勪簩绱氱祼(ji茅)
妲�(g貌u)锛氳偨鍠厓銆佄�-铻烘棆銆佄�-鎶樼枈銆佄�-杞�(zhu菐n)瑙�銆佺劇瑕�(gu墨)鍗锋洸銆佹ā搴忓強姘ㄥ熀閰稿伌(c猫)閺堝皪浜岀礆绲�(ji茅)妲�(g貌u)褰㈡垚
鐨勫奖闊�锛涜泲鐧借唱(zh矛)鐨勪笁绱氱祼(ji茅)妲�(g貌u)锛氭绱氶嵉銆佺祼(ji茅)妲�(g貌u)鍩熷強鍒嗗瓙浼翠径锛涜泲鐧借唱(zh矛)鐨勫洓绱氱祼(ji茅)妲�(g貌u)銆傝泲鐧借唱(zh矛)鐨勫垎
椤炪€�
3. 铔嬬櫧璩�(zh矛)鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳斤細铔嬬櫧璩�(zh矛)涓€绱氱祼(ji茅)妲�(g貌u)鑸囧姛鑳界殑闂�(gu膩n)绯伙細鍒嗗瓙鐥�銆傝泲鐧借唱(zh矛)绌洪枔绲�(ji茅)妲�(g貌u)鑸囧姛鑳�
鐨勯棞(gu膩n)绯伙細铔嬬櫧璩�(zh矛)妲�(g貌u)璞℃敼璁婂拰鐤剧梾銆�
4. 铔嬬櫧璩�(zh矛)鐨勭悊鍖栨€ц唱(zh矛)锛氬叐鎬цВ闆€€佽啝楂旀€ц唱(zh矛)銆佽泲鐧借唱(zh矛)璁婃€ц垏寰�(f霉)鎬с€佹矇娣€銆佺传澶栧惛鏀跺弽鎳�(y墨ng)銆�
绗簩绔� 鏍搁吀鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳�
1. 鏍搁吀鐨勫寲瀛�(xu茅)绲勬垚鍙婁竴绱氱祼(ji茅)妲�(g貌u)锛氭牳鑻烽吀鐨勭祼(ji茅)妲�(g貌u)锛氬槍鍛よ垏鍢у暥锛屾牳绯栬垏鏍歌嫹锛屾垔绯栫⒊鍘熷瓙鐨�
绶ㄨ櫉銆� 鏍搁吀鐨勪竴绱氱祼(ji茅)妲�(g貌u)锛氭蹇�銆佹牳鑻烽吀鍚勭祫鍒嗛枔鐨勯€ｆ帴閸�銆佹浉瀵柟寮忋€�
2. DNA 鐨勭┖闁撶祼(ji茅)妲�(g貌u)鑸囧姛鑳斤細DNA 鐨勪簩绱氱祼(ji茅)妲�(g貌u)鈥斺€旈洐铻烘棆绲�(ji茅)妲�(g貌u)妯″瀷锛欳hargaff 瑕�(gu墨)鍓�锛� B-DNA
闆欒灪鏃嬬祼(ji茅)妲�(g貌u)妯″瀷瑕侀粸(di菐n)銆侱NA 鐨勮秴铻烘棆绲�(ji茅)妲�(g貌u)鍙婂叾鍦ㄦ煋鑹茶唱(zh矛)涓殑绲勮锛欴NA 鐨勮秴铻烘棆绲�(ji茅)妲�(g貌u)锛屽師鏍�
鐢熺墿 DNA 鐨勯珮绱氱祼(ji茅)妲�(g貌u)銆侱NA 鍦ㄧ湡鏍哥敓鐗╃窗(x矛)鑳炴牳鍏�(n猫i)鐨勭祫瑁濓細鏍稿皬楂斻€侱NA 鐨勫姛鑳斤細鍩哄洜 锛屽熀鍥�
绲�锛孌NA 鐨勫姛鑳姐€�
3. RNA 鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳斤細淇′娇 RNA 鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳斤細hnRNA 锛宮RNA 鐨勭祼(ji茅)妲�(g貌u)鐗归粸(di菐n)銆傝綁(zhu菐n)閬�(y霉n) RNA 鐨勭祼(ji茅)
妲�(g貌u)鑸囧姛鑳斤細绋€鏈夊牽鍩�锛岃帠鐠�(hu谩n)绲�(ji茅)妲�(g貌u)锛屾皑鍩洪吀鎺ョ磵鑾�锛屽弽瀵嗙⒓瀛�锛屼笁绱氱祼(ji茅)妲�(g貌u)銆傛牳铔嬬櫧楂� RNA 鐨勭祼(ji茅)
妲�(g貌u)鑸囧姛鑳斤細鐪熸牳鍙婂師鏍哥敓鐗╂牳铔嬬櫧楂旂殑绲勬垚銆傚叾浠栧皬鍒嗗瓙 RNA锛氬嫊鐗╃窗(x矛)鑳炲収(n猫i)鍏朵粬鐨� RNA 绋
鍙婂姛鑳�銆�

  
 
4. 鏍搁吀鐨勭悊鍖栨€ц唱(zh矛)銆佽畩鎬у拰寰�(f霉)鎬у強鍏舵噳(y墨ng)鐢細鏍搁吀鐨勪竴鑸悊鍖栨€ц唱(zh矛)锛�260nm 绱鍚告敹銆侱NA
鐨勮畩鎬э細姒傚康锛岃В閺堟洸绶�锛孴m 鍊�锛屽鑹叉晥鎳�(y墨ng)銆侱NA 鐨勫京(f霉)鎬ц垏鍒嗗瓙闆滀氦锛氶€€鐏�銆�
绗笁绔�
閰�
1. 閰剁殑鍒嗗瓙绲�(ji茅)妲�(g貌u)鑸囧姛鑳斤細閰剁殑鍒嗗瓙绲勬垚锛氬柈绱旈叾锛岀祼(ji茅)鍚堥叾锛岄叾铔嬬櫧锛屽叏閰讹紝閲戝爆閰�锛岃紨閰�锛�
杓斿熀锛岀董鐢熺礌鑸囪紨閰讹紝缍敓绱犵殑鍒嗛鍙婂叾鑸囪紨閰剁殑闂�(gu膩n)绯�锛屽父瑕嬭紨閰剁殑绲�(ji茅)妲�(g貌u)鑸囧姛鑳�锛岃紨閰剁殑浣�
鐢紝閲戝爆闆㈠瓙鐨勪綔鐢�銆傞叾鐨勬椿鎬т腑蹇冿細蹇呴渶鍩哄湗(tu谩n)锛岀祼(ji茅)鍚堝熀鍦�(tu谩n)锛屽偓鍖栧熀鍦�(tu谩n)銆傚悓宸ラ叾锛氭蹇�锛孡DH
鍚屽伐閰惰瓬鐨勮畩鍖栧強鎰忕京銆�
2.
鍒讹細娲诲寲鑳斤紝瑾樺皫(d菐o)濂戝悎鍋囪锛岄劙杩戞晥鎳�(y墨ng)銆佸畾鍚戞帓鍒�銆佸鍏冨偓鍖�銆佽〃闈㈡晥鎳�(y墨ng)銆�
3. 閰朵績鍙嶆噳(y墨ng)鍕曞姏瀛�(xu茅)锛� 搴曠墿婵冨害灏嶅弽鎳�(y墨ng)閫熷害鐨勫奖闊匡細绫�-鏇兼皬鏂圭▼锛孠m銆乂max銆� 閰舵績
閰朵績鍙嶆噳(y墨ng)鐨勭壒榛�(di菐n)鑸囨(j墨)鍒讹細閰朵績鍙嶆噳(y墨ng)鐨勭壒榛�(di菐n)锛氶珮鏁堟€э紝鐗圭暟鎬�锛屽彲瑾�(di脿o)绡€(ji茅)鎬с€傞叾淇冨弽鎳�(y墨ng)姗�(j墨)
搴﹀皪鍙嶆噳(y墨ng)閫熷害鐨勫奖闊�銆� 鏈€閬╂韩搴�銆� 鏈€閬� pH銆� 鎶戝埗鍔戝皪鍙嶆噳(y墨ng)閫熷害鐨勫奖闊匡細涓嶅彲閫嗘€ф姂
鍒朵綔鐢ㄧ殑鐗归粸(di菐n)锛屽彲閫嗘€ф姂鍒朵綔鐢ㄧ殑绋銆佸崁(q奴)鍒ュ強鍕曞姏瀛�(xu茅)鐗归粸(di菐n)銆� 婵€娲诲姂灏嶅弽鎳�(y墨ng)閫熷害鐨勫奖闊匡細
蹇呴渶婵€娲诲姂锛岄潪蹇呴渶婵€娲诲姂銆� 閰舵椿鎬ф脯瀹氬強閰舵椿鎬у柈浣嶃€�
4. 閰剁殑瑾�(di脿o)绡€(ji茅)锛氶叾娲绘€х殑瑾�(di脿o)绡€(ji茅)锛氶叾鍘�锛岄叾鍘熺殑婵€娲荤殑姒傚康銆佹(j墨)鍒跺強鎰忕京銆傝畩妲�(g貌u)閰讹紝璁婃(g貌u)瑾�(di脿o)绡€(ji茅)
鑸囧崝(xi茅)鍚屾晥鎳�(y墨ng)銆傞叾鐨勫叡鍍�(ji脿)淇＞瑾�(di脿o)绡€(ji茅)姒傚康銆佺壒榛�(di菐n)鑸囨剰缇�銆傞叾鍚噺鐨勮(di脿o)绡€(ji茅)锛氶叾铔嬬櫧鍚堟垚鐨勮獦灏�(d菐o)鑸囬樆
閬忔蹇�锛岄叾闄嶈В鐨勮(di脿o)鎺�銆�
5. 閰剁殑鍛藉悕鑸囧垎椤炪€�
6. 閰惰垏閱�(y墨)瀛�(xu茅)鐨勯棞(gu膩n)绯伙細浜嗚В閰惰垏鐤剧梾鐨勯棞(gu膩n)绯�銆�
绗洓绔� 绯栦唬璎�
1. 姒傝堪锛氱硸鐨勭敓鐞嗗姛鑳�銆傜硸鐨勬秷鍖栧惛鏀� 鐗瑰畾杓夐珨杞�(zhu菐n)閬�(y霉n)鐨�銆佷富鍕曡€楄兘鐨勯亷绋�銆傜硸浠ｈ瑵鐨勬
娉�銆�
2. 绯栫殑鐒℃哀鍒嗚В锛氱硸閰佃В鐨勫弽鎳�(y墨ng)閬庣▼锛氭蹇�锛屽弽鎳�(y墨ng)閬庣▼鍙婅兘閲忕敓鎴�銆傜硸閰佃В鐨勮(di脿o)绡€(ji茅)锛氫笁鍊�
闂�(gu膩n)閸甸叾銆傜硸閰佃В鐨勭敓鐞嗘剰缇�銆�
3. 绯栫殑鏈夋哀姘у寲锛氭湁姘ф哀鍖栫殑鍙嶆噳(y墨ng)閬庣▼锛氫笁鍊嬮殠娈�锛屼笝閰吀鑴矮閰跺京(f霉)鍚堥珨鐨勭祫鎴�锛屼笁缇ч吀
寰挵(hu谩n)鐨勯亷绋嬪強鐢熺悊鎰忕京銆傛湁姘ф哀鍖栫敓鎴愮殑 ATP 銆傛湁姘ф哀鍖栫殑瑾�(di脿o)绡€(ji茅)锛氫笝閰吀鑴矮閰跺京(f霉)鍚堥珨鍙�
涓夌晶閰稿惊鐠�(hu谩n)涓笁鍊嬮棞(gu膩n)閸甸叾鐨勮(di脿o)绡€(ji茅)銆傚反鏂痉鏁堟噳(y墨ng)銆�
4. 纾烽吀鎴婄硸閫斿緫锛氱７閰告垔绯栭€斿緫鐨勫弽鎳�(y墨ng)閬庣▼锛氬弽鎳�(y墨ng)鐨勭涓€闅庢锛�6-纾烽吀钁¤悇绯栬劔姘叾鍙� 6-
纾烽吀钁¤悇绯栭吀鑴矮閰�銆傜７閰告垔绯栭€斿緫鐨勮(di脿o)绡€(ji茅)锛�6-纾烽吀钁¤悇绯栬劔姘叾鏄棞(gu膩n)閸甸叾銆傜７閰告垔绯栭€斿緫
鐨勭敓鐞嗘剰缇┿€�
5. 绯栧師鐨勫悎鎴愯垏鍒嗚В锛氱硸鍘熺殑鍚堟垚浠ｈ瑵锛歎DPG 鏄椿鎬ц憽钀勭硸渚涢珨浠ュ強鍚堟垚閬庣▼銆傜硸鍘熺殑鍒�
瑙ｄ唬璎濓細鍒嗚В閬庣▼銆傜硸鍘熷悎鎴愯垏鍒嗚В鐨勮(di脿o)绡€(ji茅)锛氱７閰稿寲閰�銆佺硸鍘熷悎閰剁殑鍏卞児(ji脿)淇＞瑾�(di脿o)绡€(ji茅)銆傜硸鍘熺疮
绌嶇櫏銆�
6. 绯栫暟鐢燂細绯栫暟鐢熼€斿緫锛氭蹇靛強绯栫暟鐢熺殑鍥涘€嬮棞(gu膩n)閸甸叾銆傜硸鐣扮敓鐨勮(di脿o)绡€(ji茅)銆傜硸鐣扮敓鐨勭敓鐞嗘剰缇�銆�
涔抽吀寰挵(hu谩n)锛氬惊鐠�(hu谩n)閬庣▼鍙婄敓鐞嗘剰缇�銆�

  
 
7. 琛€绯栧強鍏惰(di脿o)绡€(ji茅)锛氳绯栫殑渚嗘簮鍜屽幓璺�銆傝绯栨按骞崇殑瑾�(di脿o)绡€(ji茅)锛氳儼宄剁礌銆佽儼楂樿绯栫礌銆佺硸鐨唱(zh矛)婵€
绱犲強鑵庝笂鑵虹礌鍚勮嚜灏嶈绯栫殑褰遍熆銆傝绯栨按骞崇暟甯革細楂樿绯栧強绯栧翱鐥�锛屼綆琛€绯�銆�
绗簲绔� 鑴傞浠ｈ瑵
1.鑴傝唱(zh矛)鐨勭ó椤炲強鍔熻兘锛氬涓嶉＝鍜岃剛閰哥殑閲嶈琛嶇敓鐗┾€斺€斿墠鍒楄吅绱犮€佽鏍撳檨鐑峰強鐧戒笁鐑細鍓嶅垪
鑵虹礌銆佽鏍撳檨鐑峰強鐧戒笁鐑殑鍖栧(xu茅)绲�(ji茅)妲�(g貌u)銆佸懡鍚�銆佸悎鎴愬強鐢熺悊鍔熻兘銆�
2. 鑴傝唱(zh矛)鐨勬秷鍖栧拰鍚告敹锛氳剛椤炴秷鍖栫殑涓昏鍫存墍锛岃喗姹侀吀楣�銆佽儼鑴傞叾銆佽紨鑴傞叾鐨勪綔鐢紝鑴傝偑鍚�
鎴愮殑鐢樻补涓€閰€斿緫銆�
3. 鐢樻补涓夐叝浠ｈ瑵 锛氱敇娌逛笁閰殑鍚堟垚浠ｈ瑵 锛氬悎鎴愰儴浣�銆佸師鏂�銆佸悎鎴愬熀鏈亷绋嬶細鐢樻补涓€閰�
閫斿緫鍜岀敇娌逛簩閰€斿緫銆傜敇娌逛笁閰殑鍒嗚В浠ｈ瑵锛氳剛鑲殑鍕曞摗锛氭縺绱犳晱鎰熸€х敇娌逛笁閰剛鑲叾銆佽剛
瑙ｆ縺绱犺垏鎶楄剛瑙ｆ縺绱�銆傝剛閰哥殑尾-姘у寲锛氳剛鑲吀鐨勬椿鍖栤€旇剛閰� CoA 鐨勭敓鎴�锛岃剛閰� CoA 閫�(j矛n)鍏ョ窔
绮掗珨锛岃剛鑲吀鐨勎�-姘у寲锛岃剛鑲吀姘у寲鐨勮兘閲忕敓鎴�銆傞叜楂旂殑鐢熸垚鍙婂埄鐢細閰珨鐨勬蹇�锛岄叜楂�
鐨勭敓鎴�锛岄叜楂旂殑鍒╃敤锛岄叜楂旂敓鎴愮殑鐢熺悊鎰忕京锛岄叜楂旂敓鎴愮殑瑾�(di脿o)绡€(ji茅)锛岄叜鐧ラ吀涓瘨銆傝剛閰哥殑鍚堟垚浠�
璎濓細杌熻剛閰哥殑鍚堟垚锛氬悎鎴愰儴浣�銆佸師鏂欍€佽剛鑲吀鍚堟垚閰剁郴鍙婂弽鎳�(y墨ng)閬庣▼銆備笉椋藉拰鑴傝偑閰哥殑鍚堟垚 锛�
蹇呴渶鑴傝偑閰哥殑姒傚康銆傝剛鑲吀鍚堟垚鐨勮(di脿o)绡€(ji茅)锛氫唬璎濈墿鐨勮(di脿o)绡€(ji茅)锛屾縺绱犵殑瑾�(di脿o)绡€(ji茅)浣滅敤銆�
4. 纾疯剛鐨勪唬璎濓細鐢樻补纾疯剛鐨勪唬璎濓細鐢樻补纾疯剛鐨勭祫鎴愩€佸垎椤炲強绲�(ji茅)妲�(g貌u)銆傜敇娌圭７鑴傜殑鍚堟垚锛氬悎鎴�
閮ㄤ綅銆佸師鏂欏強杓斿洜瀛�锛屽悎鎴愬熀鏈亷绋�銆傜敇娌圭７鑴傜殑闄嶈В锛氱敱灏堜竴鎬т笉鍚岀殑纾疯剛閰� A1銆丄2銆�
B1銆丅2銆丆銆丏 鍒嗗垾浣滅敤銆�
5. 鑶藉浐閱囦唬璎濓細鑶藉浐閱囩殑绲�(ji茅)妲�(g貌u)锛屽垎甯冨強鐢熺悊鍔熻兘銆傝喗鍥洪唶鐨勫悎鎴愶細鍚堟垚閮ㄤ綅銆佸師鏂欙細涔欓叞
CoA銆佽兘閲忓強渚涙矮鐗╄唱(zh矛)銆傚悎鎴愬熀鏈亷绋嬶細鑶藉浐閱囧悎鎴愮殑闄愰€熼叾銆佸悎鎴愮殑鍩烘湰閬庣▼銆傝喗鍥洪唶鍚�
鎴愮殑瑾�(di脿o)绡€(ji茅)锛氶椁撳拰椋介銆佽喗鍥洪唶鍙婃縺绱犲垎鍒ョ殑瑾�(di脿o)绡€(ji茅)銆傝喗鍥洪唶鐨勮綁(zhu菐n)鍖栵細杞�(zhu菐n)鍖栨垚鑶芥眮閰�銆侀鍥洪唶
婵€绱�銆�7锛嶈劔姘喗鍥洪唶銆�
6. 琛€婕胯剛铔嬬櫧浠ｈ瑵锛氳鑴傦細琛€鑴傜殑绲勬垚鍙婂惈閲�銆傝婕胯剛铔嬬櫧鐨勫垎椤炪€佺祫鎴愬強绲�(ji茅)妲�(g貌u)銆傝級鑴傝泲
鐧�銆傝婕胯剛铔嬬櫧浠ｈ瑵锛氫钩绯滃井绮掞紝妤典綆瀵嗗害鑴傝泲鐧�锛屼綆瀵嗗害鑴傝泲鐧斤紝妤典綆瀵嗗害鑴傝泲鐧�锛岄珮瀵�
搴﹁剛铔嬬櫧銆傝婕胯剛铔嬬櫧浠ｈ瑵鐣板父锛氶珮鑴傝泲鐧借鐧�锛岄伜鍌虫€х己闄�銆�
绗叚绔� 鐢熺墿姘у寲
1. 鐢熺墿姘у寲鐨勬蹇靛強鎰忕京銆�
2锛庢哀鍖栧懠鍚搁張鐨勬蹇�锛屽叐姊濆懠鍚搁張鐨勭祫鎴愬拰鎺掑垪闋嗗簭銆�
3锛庢哀鍖栫７閰稿寲锛氭蹇�锛孭/O锛屽伓鑱�(li谩n)閮ㄤ綅锛屽伓鑱�(li谩n)姗�(j墨)鍒讹紞鍖栧(xu茅)婊查€忓亣瑾€�
4锛庡奖闊挎哀鍖栫７閰稿寲鐨勫洜绱狅細鍛煎惛閺堟姂鍒跺姂銆佽В鍋惰伅(li谩n)鍔戙€佹哀鍖栫７閰稿寲鎶戝埗鍔�锛孉DP 鐨勮(di脿o)绡€(ji茅)浣�
鐢�锛岀敳鐙€鑵烘縺绱�銆�
5锛嶢TP 鍦ㄨ兘閲忎唬璎濅腑鐨勪綔鐢細楂樿兘纾烽吀閸�锛屽父瑕嬬殑楂樿兘纾烽吀鍖栧悎鐗�锛岀敓鐗╅珨鍏�(n猫i)鑳介噺鐨勫劜瀛�
鍜屽埄鐢�銆�
6锛庨€氶亷绶氱矑楂斿収(n猫i)鑶滅殑鐗╄唱(zh矛)杞�(zhu菐n)閬�(y霉n)锛氱窔绮掗珨鍏�(n猫i)鑶滅殑涓昏杞�(zhu菐n)閬�(y霉n)铔嬬櫧锛岃優婕夸腑 NADH 鐨勬哀鍖栵紞伪-纾�
閰哥敇娌圭┛姊�銆佽構鏋滈吀-澶╁啲姘ㄩ吀绌挎銆�

  
 
绗竷绔� 姘ㄥ熀閰镐唬璎�
1. 铔嬬櫧璩�(zh矛)鐨勭嚐椁�(y菐ng)浣滅敤锛氳泲鐧借唱(zh矛)鐕熼(y菐ng)鐨勯噸瑕佹€�銆傝泲鐧借唱(zh矛)鐨勯渶瑕侀噺鍜岀嚐椁�(y菐ng)鍍�(ji脿)鍊硷細姘钩琛★紝鐢熺悊
闇€瑕侀噺, 铔嬬櫧璩�(zh矛)鐨勭嚐椁�(y菐ng)鍍�(ji脿)鍊�銆�
2. 蹇呴渶姘ㄥ熀閰哥殑姒傚康鍜岀ó椤�銆�
3. 铔嬬櫧璩�(zh矛)鐨勬秷鍖栥€佸惛鏀惰垏鑵愭晽锛氳泲鐧借唱(zh矛)鐨勬秷鍖栵細鑳冧腑鐨勬秷鍖�锛屽皬鑵镐腑鐨勬秷鍖�銆傝泲鐧借唱(zh矛)鐨勮厫
鏁椾綔鐢細鑳洪鐨勭敓鎴�锛屾皑鐨勭敓鎴�锛屽叾浠栨湁瀹崇墿璩�(zh矛)鐨勭敓鎴愩€�
4. 姘ㄥ熀閰哥殑涓€鑸唬璎� 锛氶珨鍏�(n猫i)铔嬬櫧璩�(zh矛)鐨勮綁(zhu菐n)鎻涙洿鏂帮細楂斿収(n猫i)姘ㄥ熀閰哥殑闄嶈В鍙婃皑鍩洪吀鐨勪唬璎濆韩鐨�
姒傚康銆傛皑鍩洪吀鐨勮劔姘ㄥ熀浣滅敤锛氳伅(li谩n)鍚堣劔姘ㄥ熀浣滅敤銆傝綁(zhu菐n)姘ㄥ熀浣滅敤锛氳綁(zhu菐n)姘ㄩ叾鑸囪綁(zhu菐n)姘ㄥ熀浣滅敤锛岃綁(zhu菐n)姘ㄥ熀
浣滅敤鐨勬(j墨)鍒�銆侺-璋锋皑閰告哀鍖栬劔姘ㄥ熀浣滅敤銆傚槍鍛ゆ牳鑻烽吀寰挵(hu谩n)銆偽�-閰吀鐨勪唬璎� 锛氱稉(j墨ng)姘у寲鐢熸垚
闈炲繀闇€姘ㄥ熀閰革紝杞�(zhu菐n)璁婃垚鎴愮硸鍙婅剛椤�锛屾哀鍖栦緵鑳斤細姘ㄥ熀閰搞€佺硸鍙婅剛鑲唬璎濈殑鑱�(li谩n)绯�銆�
5. 姘ㄧ殑浠ｈ瑵: 楂斿収(n猫i)姘ㄧ殑渚嗘簮锛氭皑鍩洪吀鑴皑鍩轰綔鐢ㄧ敘(ch菐n)鐢熺殑姘ㄦ槸楂斿収(n猫i)姘ㄧ殑涓昏渚嗘簮锛岃吀閬撳惛鏀�
鐨勬皑锛岃厧灏忕涓婄毊绱�(x矛)鑳炲垎娉岀殑姘ㄤ富瑕佷締鑷胺姘ㄩ叞鑳恒€傛皑鐨勮綁(zhu菐n)閬�(y霉n)锛氫笝姘ㄩ吀-钁¤悇绯栧惊鐠�(hu谩n)锛岃胺姘�
閰拌兒鐨勯亱(y霉n)姘ㄤ綔鐢ㄣ€傚翱绱犵殑鐢熸垚 锛氳倽鏄翱绱犲悎鎴愮殑涓昏鍣ㄥ畼锛屽翱绱犲悎鎴愮殑槌ユ皑閰稿惊鐠�(hu谩n)瀛�(xu茅)瑾�锛�
槌ユ皑閰稿惊鐠�(hu谩n)鐨勮┏绱�(x矛)姝ラ锛屽翱绱犲悎鎴愮殑瑾�(di脿o)绡€(ji茅)銆傞珮姘ㄨ鐧ュ拰姘ㄤ腑姣�銆�
6. 鍊嬪垾姘ㄥ熀閰哥殑浠ｈ瑵锛氭皑鍩洪吀鐨勮劔缇у熀浣滅敤锛毼�-姘ㄥ熀涓侀吀 銆佺墰纾洪吀銆佺祫鑳�銆�5-缇ヨ壊鑳�銆�
澶氳兒銆備竴纰冲柈浣嶇殑浠ｈ瑵锛氫竴纰冲柈浣嶈垏鍥涙矮钁夐吀锛屼竴纰冲柈浣嶈垏姘ㄥ熀閰镐唬璎�锛屼竴纰冲柈浣嶇殑鐩镐簰杞�(zhu菐n)
璁婏紝涓€纰冲柈浣嶇殑鐢熺悊鍔熻兘銆傚惈纭皑鍩洪吀浠ｈ瑵 锛氱敳纭皑閰哥殑浠ｈ瑵锛氱敳纭皑閰歌垏杞�(zhu菐n)鐢插熀浣滅敤锛�
鐢茬～姘ㄩ吀寰挵(hu谩n)锛岃倢閰哥殑鐢熸垚銆傚崐鑳辨皑閰歌垏鑳辨皑閰哥殑浠ｈ瑵锛氬崐鑳辨皑閰歌垏鑳辨皑閰哥殑浠ｈ瑵锛岀～閰告牴
鐨勪唬璎�銆傝姵棣欐棌姘ㄥ熀閰哥殑浠ｈ瑵 锛氳嫰涓欐皑閰稿強閰皑閰哥殑鍒嗚В浠ｈ瑵锛氬厭鑼堕厷鑳鸿垏榛戣壊绱犵殑鍚堟垚锛�
閰皑閰哥殑鍒嗚В浠ｈ瑵锛岃嫰涓欓叜閰稿翱鐧�銆傝壊姘ㄩ吀鐨勪唬璎�銆�
绗叓绔� 鏍歌嫹閰镐唬璎�
1.鍢屽懁鍜屽槯鍟舵牳鑻烽吀寰為牠鍚堟垚鍘熸枡鍙婂悎鎴愮殑閫斿緫銆�
2.鍢屽懁鍜屽槯鍟舵牳鑻烽吀瑁�(b菙)鏁戝悎鎴�銆�
3.鑴哀鏍歌嫹閰哥殑鐢熸垚銆�
4.鍢屽懁鍜屽槯鍟舵牳鑻烽吀鐨勫垎瑙ｄ唬璎濈敘(ch菐n)鐗╋細灏块吀鐨勭敓鎴�锛岀棝棰�(f膿ng)鍙婄棝棰�(f膿ng)鐨勬不鐧�锛汵H3銆丆O2銆佄�-涓�
姘ㄩ吀銆佄�-姘ㄥ熀鐣颁竵閰搞€�
5.鎶楁牳鑻烽吀浠ｈ瑵钘ョ墿鐨勭敓鍖栨(j墨)鍒�銆�
绗節绔� 闈炵嚐椁�(y菐ng)鐗╄唱(zh矛)浠ｈ瑵
1. 鑲濈殑鐢熺墿杞�(zhu菐n)鍖栦綔鐢細鐢熺墿杞�(zhu菐n)鍖栫殑姒傚康锛岀敓鐗╄綁(zhu菐n)鍖栧弽鎳�(y墨ng)鐨勪富瑕侀鍨嬨€�
2. 琛€绱呰壊鐨勭敓鐗╁悎鎴愶細鍚堟垚鍘熸枡锛岄檺閫熼叾锛屽悎鎴愰亷绋嬨€傝绱呰壊鐨勭敓鐗╁悎鎴愮殑瑾�(di脿o)绡€(ji茅)銆�
3. 鑶芥眮鑸囪喗姹侀吀鐨勪唬璎濓細鑶芥眮銆傝喗姹侀吀鐨勫垎椤烇細娓搁洟鑶芥眮閰�銆佺祼(ji茅)鍚堣喗姹侀吀銆佸垵绱氳喗姹侀吀鍜�
娆＄礆鑶芥眮閰搞€傝喗姹侀吀鐨勪唬璎濓細鍒濈礆鑶芥眮閰哥殑鐢熸垚锛屾绱氳喗姹侀吀鐨勭敓鎴愯垏鑲濊吀寰挵(hu谩n)銆傝喗姹侀吀鐨�
鍔熻兘锛氫績閫�(j矛n)鑴傞娑堝寲鍚告敹锛屾姂鍒惰喗姹佷腑鑶藉浐閱囩殑鏋愬嚭銆�

  
 
4. 鑶借壊绱犵殑浠ｈ瑵鑸囬粌鐤革細鑶界磪绱犵殑鐢熸垚鍜岃綁(zhu菐n)閬�(y霉n)銆傝喗绱呯礌鍦ㄨ倽涓殑杞�(zhu菐n)璁娿€傝喗绱呯礌鍦ㄨ吀閬撲腑鐨�
璁婂寲鍜岃喗鑹茬礌鐨勮吀鑲濆惊鐠�(hu谩n)銆傝娓呰喗绱呯礌鑸囬粌鐤革細婧惰鎬ч粌鐤�锛岃倽绱�(x矛)鑳炴€ч粌鐤�锛岄樆濉炴€ч粌鐤搞€�
绗崄绔� DNA 鐨勭敓鐗╁悎鎴愶紙寰�(f霉)鍒讹級
1. 寰�(f霉)鍒剁殑鍩烘湰瑕�(gu墨)寰嬶細鍗婁繚鐣欏京(f霉)鍒剁殑瀵�(sh铆)椹�(y脿n)渚濇摎(j霉)鍜屾剰缇┿€傞洐鍚戝京(f霉)鍒�銆傚京(f霉)鍒剁殑鍗婁笉閫ｇ簩(x霉)鎬э細寰�(f霉)鍒�
鍙�锛岄牁(l菒ng)闋張锛岄毃寰為張锛屽病宕庣墖娈�銆�
2. DNA 寰�(f霉)鍒剁殑閰跺(xu茅)鍜屾嫇?f霉)閶礧(xu茅)璁婂寲锛氬京(f霉)鍒剁殑鍖栧(xu茅)鍙嶆噳(y墨ng)锛氬弽鎳�(y墨ng)楂旂郴銆侱NA 鑱氬悎閰讹細鍘熸牳鐢熺墿銆佺湡
鏍哥敓鐗� DNA 鑱氬悎閰�銆傚京(f霉)鍒朵繚鐪熸€х殑閰跺(xu茅)渚濇摎(j霉)锛氭牳閰稿鍒囬叾娲绘€у拰鏍¤畝锛屽京(f霉)鍒剁殑淇濈湡鎬у拰鍫垮熀
閬告搰銆傚京(f霉)鍒朵腑瑙ｉ張鍜� DNA 鍒嗗瓙鎷�?f霉)閶礧(xu茅)璁婂寲锛氳В铻烘棆閰�銆佸紩鐗╅叾鍜屽柈閺� DNA 绲�(ji茅)鍚堣泲鐧�锛孌NA 鎷�
鎾茬暟妲�(g貌u)閰讹紙鈪犲瀷鍜屸叀鍨嬶級銆侱NA 閫ｆ帴閰�銆�
3锛嶥NA 鐢熺墿鍚堟垚閬庣▼锛氬師鏍哥敓鐗╃殑 DNA 鐢熺墿鍚堟垚锛氳捣濮嬶紙瑙ｉ張銆佸紩鐧�(f膩)楂斿拰寮曠墿锛�锛屽欢闀�锛�
绲傛銆傜湡鏍哥敓鐗╃殑 DNA 鐢熺墿鍚堟垚锛氱窗(x矛)鑳炲懆鏈�銆傚悎鎴愰亷绋嬶細璧峰锛屽欢闀�锛岀祩姝�銆傜绮掗叾銆�
4. 閫嗚綁(zhu菐n)閷勫拰鍏朵粬寰�(f霉)鍒舵柟寮忥細閫嗚綁(zhu菐n)閷勭梾姣掑拰閫嗚綁(zhu菐n)閷勯叾銆傞€嗚綁(zhu菐n)閷勭爺绌剁殑鎰忕京銆�
5. DNA 鎼嶅偡锛堢獊璁婏級鑸囦慨寰�(f霉)锛氱獊璁婄殑鎰忕京銆傚紩鐧�(f膩)绐佽畩鐨勫洜绱�銆傜獊璁婄殑鍒嗗瓙鏀硅畩椤炲瀷锛氶尟閰�銆�
缂哄け銆佹彃鍏ャ€佹绉荤獊璁�銆侀噸鎺�銆侱NA 鎼嶅偡鐨勪慨寰�(f霉)锛氬厜淇京(f霉)銆佸垏闄や慨寰�(f霉)銆侀噸绲勪慨寰�(f霉)銆丼OS 淇京(f霉)銆�
绗崄涓€绔� RNA 鐨勭敓鐗╁悎鎴愶紙杞�(zhu菐n)閷勶級
1. 寰�(f霉)鍒惰垏杞�(zhu菐n)閷勭殑鍗€(q奴)鍒ャ€�
2. 杞�(zhu菐n)閷勭殑妯℃澘鍜岄叾锛氳綁(zhu菐n)閷勬ā鏉匡細绲�(ji茅)妲�(g貌u)鍩哄洜锛屼笉灏嶇ū杞�(zhu菐n)閷�锛屾ā鏉块張锛岀法纰奸張銆� RNA 鑱氬悎閰讹細
鍘熸牳鐢熺墿鐨� RNA 鑱氬悎閰讹紙鏍稿績閰�銆佸叏閰讹級锛岀湡鏍哥敓鐗╃殑 RNA 鑱氬悎閰讹紙鈪�銆佲叀銆佲參锛�銆傛ā鏉胯垏
閰剁殑杈ㄨ獚(r猫n)绲�(ji茅)鍚堛€�
3. 杞�(zhu菐n)閷勯亷绋嬶細鍘熸牳鐢熺墿杞�(zhu菐n)閷勯亷绋嬶細杞�(zhu菐n)閷勮捣濮�銆佸欢闀凤紙杞�(zhu菐n)閷勭┖娉★級銆佺祩姝紙渚濊炒 Rho銆侀潪渚�
璩� Rho 鐨勮綁(zhu菐n)閷勭祩姝級銆傜湡鏍哥敓鐗╄綁(zhu菐n)閷勯亷绋嬶細杞�(zhu菐n)閷勮捣濮嬶紙TATA 鐩掓垨 Hogness 鐩�锛岃綁(zhu菐n)閷勫洜瀛�锛�
杞�(zhu菐n)閷勮捣濮嬪墠寰�(f霉)鍚堢墿锛夈€佸欢闀�銆佺祩姝紙鐪熸牳鐢熺墿杞�(zhu菐n)閷勭祩姝㈢殑淇＞榛�(di菐n)锛�銆�
4. 鐪熸牳鐢熺墿鐨勮綁(zhu菐n)閷勫悗淇＞锛氱湡鏍哥敓鐗� mRNA 鐨勮綁(zhu菐n)閷勫悗鍔犲伐锛氶銆佸熬鐨勪慨椋�锛宮RNA 鐨勫壀鎺�銆�
tRNA 鐨勮綁(zhu菐n)閷勫悗鍔犲伐锛�5鈥欏墠灏�(d菐o)搴忓垪鍒囬櫎锛岀█鏈夊牽鍩虹敓鎴愶紙鐢插熀鍖�銆侀倓鍘�銆佹牳鑻峰収(n猫i)鐨勮綁(zhu菐n)浣�銆佽劔
姘級锛�3鈥欐湯绔姞 CCA锛峅H銆俽RNA 鐨勮綁(zhu菐n)閷勫悗鍔犲伐锛�45S RNA 鍓帴銆傛牳閰讹細鏍搁叾鐨勭壒鎬э紙鏍搁叾
浣滅敤鐨勫熀绀�(ch菙)鈥旈寴闋祼(ji茅)妲�(g貌u)锛夛紝鏍搁叾鐮旂┒鐨勬剰缇┿€�
绗崄浜岀珷 铔嬬櫧璩�(zh矛)鐨勭敓鐗╁悎鎴愶紙缈昏锛�
1. 铔嬬櫧璩�(zh矛)鐢熺墿鍚堟垚楂旂郴锛氱炕璀ā鏉� mRNA 鍙婇伜鍌冲瘑纰硷細閬哄偝瀵嗙⒓鐨勬蹇�銆佺ó椤�銆佺壒榛�(di菐n)锛堟柟
鍚戞€�銆侀€ｇ簩(x霉)鎬с€佺啊骞舵€�銆佹摵鍕曟€�銆侀€氱敤鎬э級銆傛牳铔嬬櫧楂旀槸澶氳偨閺堝悎鎴愮殑瑁濈疆銆倀RNA 鍔熻兘銆�
tRNA 姘ㄥ熀閰哥殑娲诲寲锛氭皑鍩洪叞-tRNA 鍚堟垚閰�锛岃捣濮嬫皑鍩洪叞-tRNA銆�
2. 铔嬬櫧璩�(zh矛)鐢熺墿鍚堟垚閬庣▼锛氳偨閺堝悎鎴愯捣濮嬶細鍘熸牳缈昏璧峰寰�(f霉)鍚堢墿褰㈡垚锛堟牳铔嬬櫧楂斾簽鍩哄垎闆�锛�
mRNA 灏忎簽鍩哄畾浣嶇祼(ji茅)鍚�锛岃捣濮嬫皑鍩洪叞-tRNA 鐨勭祼(ji茅)鍚�锛屾牳铔嬬櫧楂斿ぇ浜炲熀绲�(ji茅)鍚堬級锛涚湡鏍哥敓鐗╃炕璀捣
濮嬪京(f霉)鍚堢墿褰㈡垚锛堟牳铔嬬櫧楂斿ぇ灏忎簽鍩哄垎闆紝璧峰姘ㄥ熀閰�-tRNA 鐨勭祼(ji茅)鍚�锛宮RNA 鍦ㄦ牳铔嬬櫧楂斿皬浜炲熀

  
 
鐨勬簴(zh菙n)纰哄氨浣�锛屾牳铔嬬櫧楂斿ぇ浜炲熀绲�(ji茅)鍚堬級銆傝偨閺堢殑寤堕暦锛氭牳铔嬬櫧楂斿惊鐠�(hu谩n)锛堥€�(j矛n)浣嶃€佹垚鑲�銆佽綁(zhu菐n)浣嶏級銆�
鑲介張鍚堟垚鐨勭祩姝細铔嬬櫧璩�(zh矛)鐢熺墿鍚堟垚閬庣▼涓殑鑳介噺娑堣€楋紝澶氳仛鏍歌泲鐧介珨銆�
3. 铔嬬櫧璩�(zh矛)鍚堟垚鍚庡姞宸ュ拰杓搁€侊細澶氳偨閺堟姌鐤婄偤澶╃劧鍔熻兘妲�(g貌u)璞＄殑铔嬬櫧璩�(zh矛)锛氬垎瀛愪即渚讹紙鐔变紤鍏嬭泲
鐧斤紝浼翠径绱狅級锛岃泲鐧戒簩纭嵉鐣版(g貌u)閰讹紝鑲斤紞鑴皑閰伴爢鍙嶇暟妲�(g貌u)閰�銆備竴绱氱祼(ji茅)妲�(g貌u)鐨勪慨椋撅細鑲介張 N 绔殑
淇＞锛屽€嬪垾姘ㄥ熀閰哥殑鍏卞児(ji脿)淇＞锛屽鑲介張鐨勬按瑙ｄ慨椋�?锛熻嚮g绲�(ji茅)妲�(g貌u)鐨勪慨椋撅細浜炲熀鑱氬悎锛岃紨鍩洪€ｆ帴锛�
鐤忔按鑴傞張鐨勫叡鍍�(ji脿)淇＞銆傝泲鐧借唱(zh矛)鍚堟垚鍚庣殑闈跺悜杓搁€侊細鍒嗘硨鎬ц泲鐧界殑闈跺悜杓搁€侊紙淇¤櫉鑲斤紝淇¤櫉鑲�
璀樺垾椤嗙矑锛孲RP 灏嶆帴铔嬬櫧锛夛紝绶氱矑楂旇泲鐧界殑闈跺悜杓搁€�锛岀窗(x矛)鑳炴牳铔嬬櫧鐨勯澏鍚戣几閫侊紙鏍稿畾浣嶅簭鍒楋級銆�
绗崄涓夌珷 鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺�
1. 鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺у熀鏈蹇佃垏鐗归粸(di菐n)锛氬熀鍥犺〃閬�(d谩)鐨勬蹇碉細鍩哄洜锛屽熀鍥犵祫锛屽熀鍥犺〃閬�(d谩)銆傚熀鍥犺〃閬�(d谩)
鐨勭壒鐣版€э細鏅傞枔鎬у強绌洪枔鎬�?锛涱媰铏_(d谩)鐨勬柟寮忥細鍩烘湰琛ㄩ仈(d谩)锛堢祫鎴愭€ц〃閬�(d谩)锛�锛岃獦灏�(d菐o)鍜岄樆閬忚〃閬�(d谩)銆�
鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺х殑澶氬堡娆℃€у拰寰�(f霉)闆滄€с€� 鍩哄洜琛ㄩ仈(d谩)鍙楅爢寮忎綔鐢ㄥ厓浠跺拰鍙嶅紡浣滅敤鍥犲瓙鍏卞悓瑾�(di脿o)绡€(ji茅)銆�
鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺х殑鐢熺墿瀛�(xu茅)鎰忕京銆�
2. 鍘熸牳鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺э細鍘熸牳鐢熺墿鍩哄洜绲勭祼(ji茅)妲�(g貌u)鐗归粸(di菐n)銆傚師鏍哥敓鐗╄綁(zhu菐n)閷勮(di脿o)鎺х殑鍩烘湰鍠綅-鎿嶇副瀛愭
蹇�銆備钩绯栨搷绺卞瓙鐨勭祼(ji茅)妲�(g貌u)锛圸銆乊 鍙� A 鍩哄洜锛屾搷绺卞簭鍒�锛屽暉鍕曞簭鍒楋紝瑾�(di脿o)绡€(ji茅)鍩哄洜锛孋AP 绲�(ji茅)鍚堜綅榛�(di菐n)锛�锛�
涔崇硸鎿嶇副瀛愯(di脿o)绡€(ji茅)姗�(j墨)鍒讹紙闃婚亸铔嬬櫧鐨勮矤(f霉)鎬ц(di脿o)绡€(ji茅)锛孋AP 鐨勬鎬ц(di脿o)绡€(ji茅)锛屽崝(xi茅)瑾�(di脿o)瑾�(di脿o)绡€(ji茅)锛�銆�
3. 鐪熸牳鍩哄洜杞�(zhu菐n)閷勮(di脿o)绡€(ji茅)锛氱湡鏍稿熀鍥犵祫绲�(ji茅)妲�(g貌u)鐗归粸(di菐n)锛氱湡鏍稿熀鍥犵祫绲�(ji茅)妲�(g貌u)榫愬ぇ锛屽柈闋嗗弽瀛�锛岄噸寰�(f霉)搴忓垪锛�
鍩哄洜涓嶉€ｇ簩(x霉)鎬�銆俁NA pol 鈪¤綁(zhu菐n)閷勮捣濮嬬殑瑾�(di脿o)绡€(ji茅)锛氶爢寮忎綔鐢ㄥ厓浠讹紙鍟熷嫊瀛�锛屽寮�(qi谩ng)瀛�锛屾矇榛樺瓙锛夛紝
鍙嶅紡浣滅敤鍥犲瓙锛堣綁(zhu菐n)閷勫洜瀛愮殑鍒嗛銆佺祼(ji茅)妲�(g貌u)锛�锛宮RNA 杞�(zhu菐n)閷勬縺娲诲強鍏惰(di脿o)绡€(ji茅)锛圱F 鈪 绲勬垚鎴愬垎鈥斺€擳BP銆�
TAF锛�銆�
绗崄鍥涚珷 绱�(x矛)鑳炰俊铏熻綁(zhu菐n)灏�(d菐o)鐨勫垎瀛愭(j墨)鍒�
1.绱�(x矛)鑳炰俊鎭墿璩�(zh矛)鐨勬蹇靛強鍒嗛銆�
2.鍙楅珨鐨勬蹇�銆佸垎椤炲拰浣滅敤鐗归粸(di菐n)銆�
3.G 铔嬬櫧銆�
4.鑶滃彈楂斾粙灏�(d菐o)鐨勪俊铏熻綁(zhu菐n)灏�(d菐o)姗�(j墨)鍒讹細cAMP-铔嬬櫧婵€閰堕€斿緫銆�
5.鑳炲収(n猫i)鍙楅珨浠嬪皫(d菐o)鐨勪俊铏熻綁(zhu菐n)灏�(d菐o)姗�(j墨)鍒讹細鐢茬媭鑵虹礌銆侀鍥洪唶婵€绱犵殑瑾�(di脿o)绡€(ji茅)閬庣▼銆�
绗崄浜旂珷 DNA 閲嶇祫鍙婇噸绲� DNA 鎶€琛�(sh霉)
1.鑷劧鐣� DNA 鐨勯噸绲勫拰鍩哄洜杞�(zhu菐n)绉讳富瑕佹柟寮忥細鍚屾簮閲嶇祫銆傜窗(x矛)鑿岀殑鍩哄洜杞�(zhu菐n)绉昏垏閲嶇祫锛氭帴鍚堜綔鐢�锛�
杞�(zhu菐n)鍖栦綔鐢�锛岃綁(zhu菐n)灏�(d菐o)浣滅敤銆傜壒鐣颁綅榛�(di菐n)閲嶇祫锛毼诲櫖鑿岄珨 DNA 鐨勬暣鍚堬紝绱�(x矛)鑿岀殑鐗圭暟浣嶉粸(di菐n)閲嶇祫銆傝綁(zhu菐n)搴ч噸
绲�銆�
2. 閲嶇祫 DNA 鎶€琛�(sh霉)鐩搁棞(gu膩n)姒傚康锛氶噸绲� DNA锛圖NA 鍏嬮殕銆佸熀鍥犲厠?銆�?锛岄檺鍒舵€ф牳閰稿収(n猫i)鍒囬叾锛岀洰鐨勫熀
鍥�锛屽熀鍥犺級楂旓紙璩�(zh矛)绮�銆佸櫖鑿岄珨锛�銆�
3锛庨噸绲� DNA 鎶€琛�(sh霉)鍩烘湰鍘熺悊鍙婃搷浣滄椹燂細鐩殑鍩哄洜鐨勭嵅?銆傚く鐦淲(xu茅)鍚堟垚娉�锛屽熀鍥犵祫 DNA 鏂囧韩锛�
cDNA 鏂囧韩锛岃仛鍚堥叾閺堝弽鎳�(y墨ng)銆傚厠闅嗚級楂旂殑閬告搰鍜屾(g貌u)寤�銆傚婧愬熀鍥犺垏杓夐珨鐨勯€ｆ帴銆傞噸绲� DNA 灏�(d菐o)
鍏ュ彈楂旇弻锛氭劅鍙楁厠(t脿i)绱�(x矛)鑳�锛屾柟寮忊€旇綁(zhu菐n)鍖�銆佽綁(zhu菐n)鏌撳拰鎰熸煋銆傞噸绲勯珨鐨勭閬�銆�