2021骞�鍖椾含甯寖澶у(xu茅)纰╁＋鐮旂┒鐢熸嫑鐢熻€冭│鑰冪爺澶х侗

鐢熺墿鍖栧(xu茅)鑸囧垎瀛愮敓鐗╁(xu茅)鑰冭│澶х侗

涓€銆佽€冭│瑕佹眰

涓昏鑰冩牳瀛�(xu茅)鐢熷皪(du矛)鐢熺墿鍖栧(xu茅)鑸囧垎瀛愮敓鐗╁(xu茅)鐩搁棞(gu膩n)鐞嗚珫鐭ヨ瓨(sh铆)鐨勭悊瑙ｈ垏鎺屾彙锛屽皪(du矛)鐝�(xi脿n)浠ｇ敓鐗╁寲瀛�(xu茅)鑸囧垎瀛愮敓鐗╁(xu茅)鐮旂┒鏂规硶鍜屾妧琛�(sh霉)鐨勭悊瑙ｅ拰鎳�(y墨ng)鐢�锛屽悓鏅�(sh铆)鑰冨療瀛�(xu茅)鐢熺稖鍚堥亱(y霉n)鐢ㄦ墍瀛�(xu茅)鐭ヨ瓨(sh铆)鍒嗘瀽瀵�(sh铆)闅涚敓鐗╁(xu茅)鍟�(w猫n)椤岋紝骞舵彁鍑鸿В姹烘柟妗堢殑鎬濈董鑳藉姏銆�

浜�銆佽€冭│鍏�(n猫i)瀹�

1. 绯栭(l猫i)

鍠硸銆佸柈绯栬鐢熺墿銆佸绯�銆佸绯栧拰绯栧京(f霉)鍚堢墿鐨勬蹇�銆佹€ц唱(zh矛)銆佺祼(ji茅)妲�(g貌u)鑸囧姛鑳斤紝绯栫殑鐮旂┒鏂规硶鑸囩爺绌堕€�(j矛n)灞曠瓑銆�

2. 鑴傝唱(zh矛)

鑴傝偑閰�銆佺敇娌逛笁閰€佺７鑴�銆佺硸鑴�銆佽喗鍥洪唶绛夎剛璩�(zh矛)鍒嗗瓙鐨勬蹇�銆佹€ц唱(zh矛)銆佺祼(ji茅)妲�(g貌u)鑸囧姛鑳�锛岃剛鐨勭爺绌舵柟娉曡垏鐮旂┒閫�(j矛n)灞曠瓑銆�

3. 铔嬬櫧璩�(zh矛)

姘ㄥ熀閰哥殑姒傚康銆佹€ц唱(zh矛)銆佺祼(ji茅)妲�(g貌u)鑸囧姛鑳�锛屽鑲界殑姒傚康銆佹€ц唱(zh矛)銆佺祼(ji茅)妲�(g貌u)鑸囧姛鑳�锛岃泲鐧借唱(zh矛)鐨勬蹇�銆佹€ц唱(zh矛)銆佺祼(ji茅)妲�(g貌u)鑸囧姛鑳�锛岃偨閸�銆佹矮閸�銆佺枏姘翠綔鐢�銆佽寖寰疯彲鍔涜垏闆㈠瓙閸电殑姒傚康銆佷綔鐢ㄦ(j墨)鍒朵互鍙婂皪(du矛)铔嬬櫧璩�(zh矛)绲�(ji茅)妲�(g貌u)鑸囧姛鑳界殑褰遍熆浣滅敤锛岃泲鐧借唱(zh矛)鐨勭爺绌舵柟娉曡垏鐮旂┒閫�(j矛n)灞曠瓑銆�

4. 閰�

閰剁殑姒傚康銆佸寲瀛�(xu茅)绲勬垚銆佺祼(ji茅)妲�(g貌u)銆佹€ц唱(zh矛)鑸囧姛鑳�锛岄叾鐨勪綔鐢ㄦ(j墨)鍒�锛岄叾鐨勮(di脿o)鎺ф(j墨)鍒�锛岄叾鐨勬噳(y墨ng)鐢紝閰朵績鍙嶆噳(y墨ng)鍕�(d貌ng)鍔涘(xu茅)锛岄叾鐨勭爺绌舵柟娉曡垏鐮旂┒閫�(j矛n)灞曠瓑銆�

5. 鏍搁吀

鏍歌嫹閰哥殑姒傚康銆佹€ц唱(zh矛)銆佺祼(ji茅)妲�(g貌u)鑸囧姛鑳�锛�DNA鐨勬蹇点€佹€ц唱(zh矛)銆佺祼(ji茅)妲�(g貌u)鑸囧姛鑳�锛�RNA鐨勬蹇�銆佹€ц唱(zh矛)銆佺祼(ji茅)妲�(g貌u)鑸囧姛鑳斤紝鏍搁吀鐨勭爺绌舵柟娉曡垏鐮旂┒閫�(j矛n)灞曠瓑銆�

6. 鏂伴櫝浠ｈ瑵鑸囦唬璎濊(di脿o)鎺�

鏂伴櫝浠ｈ瑵鐨勬蹇佃垏鍩烘湰閫斿緫锛岀墿璩�(zh矛)浠ｈ瑵鑸囪兘閲忎唬璎濈殑鐩搁棞(gu膩n)姒傚康鑸囧熀鏈師鐞嗭紱绯栦唬璎濋€斿緫锛堢硸閰佃В銆佷笝閰吀鐨勬哀鍖栬劔缇у熀浣滅敤銆佷笁缇ч吀寰挵(hu谩n)銆佹垔绯栫７閰搁€斿緫銆佺硸鐣扮敓銆佷箼閱涢吀寰挵(hu谩n)銆佺硸鍘熶唬璎濈瓑锛夌殑姒傚康銆佷唬璎濋亷(gu貌)绋嬨€佺浉闂�(gu膩n)閰剁殑浣滅敤姗�(j墨)鍒�銆佽兘閲忚畩鍖栥€佽(di脿o)鎺ф(j墨)鍒惰垏鐢熺悊鎰忕京锛涜剛浠ｈ瑵閫斿緫锛堣剛鑲吀鐨勫垎瑙ｈ垏鍚堟垚浠ｈ瑵锛岀敇娌逛笁閰殑浠ｈ瑵锛岀７鑴傜殑浠ｈ瑵锛岄叜楂旂殑浠ｈ瑵锛岃喗鍥洪唶鐨勪唬璎濓級鐨勬蹇�銆佷唬璎濋亷(gu貌)绋�銆佺浉闂�(gu膩n)閰剁殑浣滅敤姗�(j墨)鍒躲€佽兘閲忚畩鍖�銆佽(di脿o)鎺ф(j墨)鍒惰垏鐢熺悊鎰忕京锛涜泲鐧借唱(zh矛)浠ｈ瑵閫斿緫锛堣泲鐧借唱(zh矛)鐨勫垎瑙ｄ唬璎�銆佹皑鍩洪吀鐨勫垎瑙ｄ唬璎濊垏鍚堟垚浠ｈ瑵锛夌殑姒傚康銆佷唬璎濋亷(gu貌)绋嬨€佺浉闂�(gu膩n)閰剁殑浣滅敤姗�(j墨)鍒�銆佽兘閲忚畩鍖�銆佽(di脿o)鎺ф(j墨)鍒惰垏鐢熺悊鎰忕京锛涙牳閰镐唬璎濋€斿緫锛堟牳鑻烽吀鐨勫垎瑙ｄ唬璎濊垏鍚堟垚浠ｈ瑵锛夌殑姒傚康銆佷唬璎濋亷(gu貌)绋�銆佺浉闂�(gu膩n)閰剁殑浣滅敤姗�(j墨)鍒�銆佽兘閲忚畩鍖�銆佽(di脿o)鎺ф(j墨)鍒惰垏鐢熺悊鎰忕京锛涗唬璎濋€斿緫涔嬮枔鐨勮伅(li谩n)绯�锛涙縺绱犲皪(du矛)浠ｈ瑵鐨勮(di脿o)绡€(ji茅)浣滅敤锛涗唬璎濈殑鐮旂┒鏂规硶鑸囩爺绌堕€�(j矛n)灞曠瓑銆�

7. 姘у寲纾烽吀鍖栬垏鍏夊悎纾烽吀鍖�

鐢熺墿姘у寲銆侀浕瀛愬偝閬�銆佸懠鍚搁張銆佹哀鍖栫７閰稿寲銆佸厜鍚堢７閰稿寲鐨勬蹇碉紝姘у寲閭勫師闆诲嫝(sh矛)鐨勬蹇佃垏鎳�(y墨ng)鐢�锛屽懠鍚搁張鐨勭祫鎴愯垏闆诲瓙鍌抽仦鐨勯€斿緫锛岄浕瀛愬偝閬炶垏ATP鍚堟垚鐨勫伓鑱�(li谩n)姗�(j墨)鍒讹紝ATP鍚堥叾鐨勭祼(ji茅)妲�(g貌u)绲勬垚鑸囦綔鐢ㄦ(j墨)鍒�锛岄潪寰挵(hu谩n)寮忓厜鍚堢７閰稿寲鑸囧惊鐠�(hu谩n)寮忓厜鍚堢７閰稿寲鐨勬(j墨)鍒讹紝姘у寲纾烽吀鍖栬垏鍏夊悎纾烽吀鍖栫殑瑾�(di脿o)鎺ф(j墨)鍒�銆�

8. DNA鑸囨煋鑹查珨

閬哄偝鐗╄唱(zh矛)鐨勭壒鎬�锛涙煋鑹查珨銆佸熀鍥犵祫鍜屽熀鍥犵殑姒傚康鍜岀浉浜掗棞(gu膩n)绯伙紱鐪熸牳鍜屽師鏍哥敓鐗╁熀鍥犵祫鐨勫樊鐣�銆�

9. DNA鐨勫京(f霉)鍒�

DNA鍗婁繚鐣欏京(f霉)鍒剁殑璀夋摎(j霉)锛涚悊瑙ｅ師鏍稿拰鐪熸牳DNA寰�(f霉)鍒剁殑姗�(j墨)鍒�锛涙帉鎻″弮鑸�DNA寰�(f霉)鍒剁殑铔嬬櫧鍒嗗瓙鐨勪綔鐢紱绔矑鍜岀绮掗叾鐨勬蹇靛強鍏剁敓鐗╁(xu茅)鎰忕京銆�

10. 杞�(zhu菐n)閷�

杞�(zhu菐n)閷勭殑鍩烘湰姒傚康锛涘師鏍稿拰鐪熸牳杞�(zhu菐n)閷勭殑璧峰銆佸欢浼稿拰绲傛锛涘師鏍稿拰鐪熸牳杞�(zhu菐n)閷勭殑姗�(j墨)鐞�锛�RNA鐨勪慨椋�銆佸壀鎺ュ拰鍔犲伐锛涙牳閰剁殑鐧�(f膩)鐝�(xi脿n)鍙婂叾鐢熺墿瀛�(xu茅)鎰忕京銆�

11. 閬哄偝瀵嗙⒓鍜岃泲鐧借唱(zh矛)鐨勭敓鐗╁悎鎴�

缈昏鐨勫熀鏈蹇碉紱鏍哥硸楂旂殑绲�(ji茅)妲�(g貌u)鍜屽姛鑳�锛涘師鏍稿拰鐪熸牳铔嬬櫧璩�(zh矛)鐢熺墿鍚堟垚閬�(gu貌)绋嬶紱閬哄偝瀵嗙⒓锛涘師鏍稿拰鐪熸牳缈昏鐨勬(j墨)鐞�锛涜泲鐧借唱(zh矛)淇＞銆佹姌鐤婂拰杞�(zhu菐n)閬�(y霉n)锛涙硾绱犲強鍏跺湪铔嬬櫧璩�(zh矛)闄嶈В涓殑浣滅敤銆�

12. 杞�(zhu菐n)搴у瓙

杞�(zhu菐n)搴у瓙鐨勫熀鏈蹇�锛涜綁(zhu菐n)搴х殑鍒嗗瓙姗�(j墨)鍒�锛涜綁(zhu菐n)搴у瓙鐨勯(l猫i)鍨嬪拰杞�(zhu菐n)搴у瓙鐨勯伜鍌虫晥鎳�(y墨ng)銆�

13. 鍩哄洜绲勫(xu茅)鍜岀郴绲�(t菕ng)鐢熺墿瀛�(xu茅)

鍩哄洜绲勫(xu茅)鍜岃泲鐧借唱(zh矛)绲勫(xu茅)涓殑鍩烘湰鎶€琛�(sh霉)鎵嬫锛涚郴绲�(t菕ng)鐢熺墿瀛�(xu茅)鐨勫熀鏈煡璀�(sh铆)鍜屾€濈董鏂瑰紡锛涚郴绲�(t菕ng)鐢熺墿瀛�(xu茅)鍜岀敓鐗╀俊鎭(xu茅)鐨勭櫦(f膩)灞曞拰鐝�(xi脿n)鐙€銆傞棞(gu膩n)閸佃(sh霉)瑾�(y菙)鎴栨蹇电殑涓嫳鏂囷紙濡傚柈鏍歌嫹閰稿鎱�(t脿i)鎬�銆佽泲鐧借唱(zh矛)绲勭瓑锛夛紱浜洪(l猫i)鍩哄洜绲勮▓(j矛)鍔冪殑鎰忕京锛涗汉椤�(l猫i)鑸囧叾瀹冪敓鐗╀笉鍚岀殑鍘熷洜銆�

14. DNA绐佽畩銆佹悕鍌峰拰淇京(f霉)

绐佽畩鐨勬蹇靛拰椤�(l猫i)鍨�锛涚獊璁婄櫦(f膩)鐢熺殑姗�(j墨)鍒讹紱DNA鎼嶅偡鐨勯(l猫i)鍨�锛�DNA鎼嶅偡淇京(f霉)鐨勬(j墨)鍒�锛�SOS鍙嶆噳(y墨ng)鐨勮(di脿o)鎺ф(j墨)鐞�銆�

15. 鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺�

鍘熸牳鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺х殑鍩烘湰瑕�(gu墨)寰�锛涙搷绺卞瓙鍜屾搷绺卞瓙鐨勮(di脿o)鎺ф(j墨)鍒�锛涚湡鏍稿熀鍥犵祫鐨勭壒榛�(di菐n)锛涚湡鏍�DNA姘村钩銆佽綁(zhu菐n)閷勬按骞冲拰杞�(zhu菐n)閷勫悗姘村钩鐨勫熀鍥犺〃閬�(d谩)瑾�(di脿o)鎺ф(j墨)鍒�锛涘熀鍥犳矇榛樺拰鍩哄洜琛ㄩ仈(d谩)鐨勮〃瑙€閬哄偝瑾�(di脿o)鎺ф(j墨)鍒�锛�RNAi鎶€琛�(sh霉)鐨勪綔鐢ㄦ(j墨)鐞�銆�

16. 鍒嗗瓙鐢熺墿瀛�(xu茅)鎶€琛�(sh霉)

DNA鍜岃泲鐧介浕娉虫妧琛�(sh霉)锛�DNA闄愬埗鎬�?x煤n)?n猫i)鍒囬叾锛�DNA閲嶇祫鐨勬蹇靛拰瑕佺礌锛涜級楂旇ō(sh猫)瑷�(j矛)鍜屽熀鍥犳枃搴�(k霉)鐨勬(g貌u)寤�锛涢噸绲勫瓙鐨勭閬告柟娉�锛�PCR鎶€琛�(sh霉)鐨勫師鐞嗗拰鎳�(y墨ng)鐢�锛�DNA搴忓垪娓�(c猫)瀹氱殑鏂规硶鍜屽師鐞�锛涜泲鐧�-铔嬬櫧鐩镐簰浣滅敤/铔嬬櫧-鏍搁吀锛�DNA鎴�RNA锛夌浉浜掍綔鐢ㄧ殑妾㈡脯(c猫)鎶€琛�(sh霉)锛涘熀鍥犵法杓殑鍩烘湰鍘熺悊鍜屾柟娉�锛涘悎鎴愮敓鐗╁(xu茅)鐨勬蹇靛拰鍩烘湰鍘熺悊锛涘熀鍥犲拰鍩哄洜绲勫伐绋嬪強鍏舵噳(y墨ng)鐢�銆�

涓�銆佽€冭│鏂规硶

闁夊嵎鑰冭│锛岀附鍒�150鍒嗐€�

椤屽瀷锛氬悕瑭炶В閲�銆佸～绌洪銆侀伕鎿囬銆佸晱(w猫n)绛旈銆�

鍥�銆佸弮鑰冩浉(sh奴)

銆婄敓鐗╁寲瀛�(xu茅)銆嬶紙绗�4鐗堬級锛屾湵鍦ｅ簹锛屽緪闀�(zh菐ng)娉曪紝楂樼瓑鏁欒偛鍑虹増绀�銆�

銆婄敓鐗╁寲瀛�(xu茅)鏁欑▼銆�锛岀帇閺″窎锛屾湵鍦ｅ簹锛屽緪闀�(zh菐ng)娉�锛岄珮绛夋暀鑲插嚭鐗堢ぞ銆�

銆婄従(xi脿n)浠ｅ垎瀛愮敓鐗╁(xu茅)銆嬶紙绗洓鐗堟垨绗簲鐗堬級锛屾湵鐜夎尝锛屾潕姣�锛岄劖鏇夊嘲锛岄儹绱呰(w猫i)锛岄珮绛夋暀鑲插嚭鐗堢ぞ銆�