婀栧崡甯寖澶у(xu茅)纰╁＋鐮旂┒鐢熷叆瀛�(xu茅)鑰冭│鑷懡椤岃€冭│澶х侗

鑰冭│绉戠洰浠ｇ⒓锛歔    ]               鑰冭│绉戠洰鍚嶇ū锛氬垎瀛愮敓鐗╁(xu茅)

涓€銆佽€冭│鍏�(n猫i)瀹硅垏鑰冭│瑕佹眰

1銆佸熀鍥犲拰鍩哄洜绲�

鑰冭│鍏�(n猫i)瀹�

鍩哄洜鍜屽熀鍥犵祫鐨勬蹇点€€鐥呮瘨鍩哄洜绲勭殑绲�(ji茅)妲�(g貌u)鑸囧姛鑳� 鍘熸牳鐢熺墿鍩哄洜绲� 鐪熸牳鐢熺墿鍩哄洜绲� 浜洪鍩哄洜绲勭殑鐗归粸(di菐n)鍜屼汉椤炲熀鍥犵祫瑷�(j矛)鍔� 鍩哄洜绲勫(xu茅)

鑰冭│瑕佹眰

锛�1锛夌悊瑙ｅ熀鍥犲拰鍩哄洜绲勭殑姒傚康锛屾帉鎻″熀鍥犵殑绲�(ji茅)妲�(g貌u)銆�

锛�2锛夋帉鎻＄梾姣掑熀鍥犵祫鐨勭祼(ji茅)妲�(g貌u)鐗归粸(di菐n)锛岀悊瑙ｄ箼鑲濈梾姣掑拰鍙嶈綁(zhu菐n)閷勭梾姣掑熀鍥犵祫銆�

锛�3锛変簡瑙ｅ師鏍哥敓鐗╁熀鍥犵祫绲�(ji茅)妲�(g貌u)鑸囧姛鑳界壒榛�(di菐n)锛屾帉鎻¤唱(zh矛)绮掔殑姒傚康锛岀悊瑙ｈ唱(zh矛)绮掔殑鍒嗛鍜岀壒鎬�锛涙帉鎻¤綁(zhu菐n)搴т綔鐢ㄧ殑姒傚康锛岀悊瑙ｈ綁(zhu菐n)搴т綔鐢ㄧ殑姗�(j墨)鍒跺拰閬哄偝瀛�(xu茅)鏁堟噳(y墨ng)銆�

锛�4锛変簡瑙ｇ湡鏍哥敓鐗╁熀鍥犵祫鐨勭壒榛�(di菐n)锛屾帉鎻″熀鍥犲鏃忕殑姒傚康锛岀悊瑙ｅ熀鍥犲鏃忎腑鍚勫熀鍥犻枔鐨勯棞(gu膩n)绯诲拰鐗归粸(di菐n)銆�

锛�5锛変簡瑙ｄ汉椤炲熀鍥犵祫鐨勭壒榛�(di菐n)锛屾帉鎻′汉椤炲熀鍥犵祫瑷�(j矛)鍔冪殑鍏�(n猫i)瀹�銆�

锛�6锛夋帉鎻″熀鍥犵祫瀛�(xu茅)鐨勬蹇�锛屼簡瑙ｅ熀鍥犵祫瀛�(xu茅)鐨勫収(n猫i)瀹�锛岀悊瑙ｅ姛鑳藉熀鍥犵祫瀛�(xu茅)鐨勫収(n猫i)瀹�銆�

2銆丏NA搴忓垪鍒嗘瀽

鑰冭│鍏�(n猫i)瀹�

闆欒劔姘ф湯绔祩姝㈡硶  鍖栧(xu茅)闄嶈В娉�  DNA鑷嫊(d貌ng)娓�(c猫)搴�

鑰冭│瑕佹眰

锛�1锛夋帉鎻￠洐鑴哀鏈绲傛娉曟脯(c猫)搴忕殑鍩烘湰鍘熺悊鍜屼富瑕佹椹�銆�

锛�2锛夌悊瑙ｅ寲瀛�(xu茅)瑁傝В娉曟脯(c猫)搴忕殑鍩烘湰鍘熺悊锛屾帉鎻″寲瀛�(xu茅)瑁傝В娉曟脯(c猫)搴忕殑涓昏鎿嶄綔姝ラ銆�

锛�3锛夌悊瑙NA鑷嫊(d貌ng)娓�(c猫)搴忕殑鍩烘湰鍘熺悊鍜屼富瑕佹噳(y墨ng)鐢�锛屼簡瑙ｆ脯(c猫)搴忓湒璀滅殑闁辫畝鏂规硶銆�

3銆佹牳閰稿垎瀛愰洔浜�

鑰冭│鍏�(n猫i)瀹�

鏍搁吀鍒嗗瓙闆滀氦鐨勫師鐞嗐€€Southern 鍗拌贰闆滀氦  Northern 鍗拌贰闆滀氦  鏂戦粸(di菐n)闆滀氦鍘熶綅闆滀氦

鑰冭│瑕佹眰

锛�1锛夋帉鎻℃牳閰稿垎瀛愰洔浜ょ殑姒傚康锛岀悊瑙ｆ牳閰稿垎瀛愰洔浜ょ殑鍘熺悊銆�

 锛�2锛夋帉鎻outhern 鍗拌贰闆滀氦涓昏鎿嶄綔姝ラ锛屼簡瑙outhern 鍗拌贰闆滀氦鐨勪富瑕佹噳(y墨ng)鐢�銆�

 锛�3锛夌悊瑙orthern 鍗拌贰闆滀氦鐨勬搷浣滄椹熷拰涓昏鎳�(y墨ng)鐢�銆�

 锛�4锛夋帉鎻℃枒榛�(di菐n)闆滀氦鐨勬蹇靛拰鍘熺悊锛岀悊瑙ｆ枒榛�(di菐n)闆滀氦鐨勬搷浣滄椹熷拰涓昏鎳�(y墨ng)鐢�銆�

 锛�5锛夋帉鎻″師浣嶉洔浜ょ殑姒傚康鍜屽師鐞�锛岀悊瑙ｅ師浣嶉洔浜ょ殑鎿嶄綔姝ラ鍜屼富瑕佹噳(y墨ng)鐢�銆�

4銆佽仛鍚堥叾閺堝弽鎳�(y墨ng)

鑰冭│鍏�(n猫i)瀹�

鑱氬悎閰堕張鍙嶆噳(y墨ng)锛圥CR锛夌殑鍩烘湰鍘熺悊銆€鑰愮啽鐨凞NA鑱氬悎閰躲€€ 寮曠墿瑷�(sh猫)瑷�(j矛)鐨勫熀鏈師鍓�  PCR鍙嶆噳(y墨ng)姊濅欢鐨勫劒(y艒u)鍖� RT-PCR  瀵�(sh铆)鏅�(sh铆)鐔掑厜瀹氶噺PCR  宸㈠紡PCR  鍘熶綅PCR 澶氶噸PCR 

鑰冭│瑕佹眰

锛�1锛夌悊瑙CR鐨勫畾缇�锛屾帉鎻CR鐨勫師鐞嗗拰鍩烘湰閬�(gu貌)绋�锛屼簡瑙ｅ钩鑷�(t谩i)鏈熷拰骞宠嚭(t谩i)鏁堟噳(y墨ng)銆�

锛�2锛変簡瑙ｈ€愮啽DNA鑱氬悎閰跺強鍏剁壒榛�(di菐n)銆�

锛�3锛夌悊瑙ｅ紩鐗╄ō(sh猫)瑷�(j矛)鐨勫熀鏈師鍓�銆�

锛�4锛夊(xu茅)鏈�(hu矛)鍎�(y艒u)鍖朠CR鐨勫弽鎳�(y墨ng)姊濅欢銆�

锛�5锛夋帉鎻T-PCR鐨勫師鐞�锛屼簡瑙ｅ叾涓昏鎳�(y墨ng)鐢�銆�

锛�6锛夋帉鎻″(sh铆)鏅�(sh铆)鐔掑厜瀹氶噺PCR鐨勫師鐞�锛岀悊瑙ｅ叾涓昏鐨勬帰閲濇ā寮忓拰妾㈡脯(c猫)鏂规硶锛屼簡瑙ｅ叾鐗归粸(di菐n)鍜屼富瑕佹噳(y墨ng)鐢�銆�

锛�7锛夌悊瑙ｅ发寮廝CR鍜屽閲峆CR鐨勫師鐞嗗拰涓昏鎳�(y墨ng)鐢�銆�

锛�8锛夋帉鎻″師浣峆CR鐨勬蹇靛拰鍘熺悊锛岀悊瑙ｅ叾鎿嶄綔鏂规硶鍜屼富瑕佹噳(y墨ng)鐢�銆�

5銆佸熀鍥犺姱鐗�

鑰冭│鍏�(n猫i)瀹�

鍩哄洜鑺墖鐨勫埗鍌欍€€鍩哄洜鑺墖鎶€琛�(sh霉)  鍩哄洜鑺墖鎶€琛�(sh霉)鐨勪富瑕佹噳(y墨ng)鐢� 

鑰冭│瑕佹眰

锛�1锛夌悊瑙ｅ熀鍥犺姱鐗囩殑涓昏鍒跺倷鏂规硶銆�.

锛�2锛夋帉鎻″熀鍥犺姱鐗囨妧琛�(sh霉)鐨勬娓�(c猫)鍘熺悊鍜屼富瑕佹搷浣滄椹熴€�

锛�3锛夋帉鎻″熀鍥犺姱鐗囨妧琛�(sh霉)鐨勪富瑕佹噳(y墨ng)鐢�銆�

6銆佸厤鐤嵃璺℃妧琛�(sh霉)

鑰冭│鍏�(n猫i)瀹�

鍏嶇柅鍗拌贰鎶€琛�(sh霉)鐨勫師鐞�  鍏嶇柅鍗拌贰鎶€琛�(sh霉)鐨勬搷浣滄椹熴€€ 

鑰冭│瑕佹眰

锛�1锛夌悊瑙ｅ厤鐤嵃璺℃妧琛�(sh霉)鐨勫熀鏈師鐞�锛屾帉鎻″厤鐤嵃璺℃妧琛�(sh霉)鐨勪富瑕佹搷浣滄椹�锛屼簡瑙ｅ厤鐤嵃璺℃妧琛�(sh霉)鐨勪富瑕佹噳(y墨ng)鐢ㄣ€�

7銆佸熀鍥犲伐绋嬭垏楂斿琛ㄩ仈(d谩)

鑰冭│鍏�(n猫i)瀹�

銆€鍩哄洜鍏嬮殕鐨勫伐鍏烽叾 鍩哄洜鍏嬮殕鐨勮級楂�  鍩哄洜鍏嬮殕鐨勫熀鏈亷(gu貌)绋�  鐪熸牳绱�(x矛)鑳炵殑杞�(zhu菐n)鏌�  鍩哄洜鐨勬敼閫� 鍏嬮殕鍩哄洜鐨勮〃閬�(d谩)

鑰冭│瑕佹眰

锛�1锛夌悊瑙ｅ熀鍥犲厠闅嗗拰鍩哄洜宸ョ▼鐨勬蹇�锛屼簡瑙ｅ熀鍥犲厠闅嗙殑鐩殑銆�

锛�2锛夌悊瑙ｉ檺鍒舵€ф牳閰稿収(n猫i)鍒囬叾鐨勫垎椤�銆佷綔鐢ㄧ壒榛�(di菐n)鍜屼富瑕佹噳(y墨ng)鐢紝浜嗚В闄愬埗鎬ф牳閰稿収(n猫i)鍒囬叾鐨勫懡鍚�銆�

锛�3锛夌悊瑙ｈ級楂旂殑姒傚康锛屾帉鎻″厠闅嗚級楂斿拰琛ㄩ仈(d谩)杓夐珨鐨勪富瑕佹(g貌u)浠跺拰鐗归粸(di菐n)锛屼簡瑙ｄ富瑕佺殑鍏嬮殕杓夐珨鍜岃〃閬�(d谩)杓夐珨銆�

锛�4锛夋帉鎻″熀鍥犲厠闅嗙殑鍩烘湰姝ラ锛岀悊瑙ｇ洰鐨勫熀鍥犵嵅鍙栫殑涓昏鏂规硶鍙婇噸绲勫瓙鐨勭閬稿拰閼掑畾鐨勪富瑕佹柟娉�锛屾帉鎻¤綁(zhu菐n)鍖�銆佽綁(zhu菐n)鏌撳拰鎰熸煋鐨勬蹇�銆�

锛�5锛夋帉鎻＄湡鏍哥窗(x矛)鑳炶綁(zhu菐n)鏌撶殑涓昏鏂规硶锛岀悊瑙ｈ綁(zhu菐n)鏌撶殑鐪熸牳绱�(x矛)鑳炵閬哥殑涓昏鏂规硶銆�

锛�6锛夋帉鎻″熀鍥犲畾榛�(di菐n)瑾樿畩鐨勪富瑕佹柟娉�銆備簡瑙ｅ熀鍥犲畾榛�(di菐n)瑾樿畩鐨勪富瑕佹噳(y墨ng)鐢�銆�

锛�7锛夌悊瑙ｅ厠闅嗗熀鍥犲師鏍歌〃閬�(d谩)鐨勫熀鏈绱犲拰鎻愰珮琛ㄩ仈(d谩)姘村钩鐨勪富瑕佹帾鏂�锛屼簡瑙ｅ嫊(d貌ng)鐗╃窗(x矛)鑳炶〃閬�(d谩)绯荤当(t菕ng)銆佹槅锜�(ch贸ng)绱�(x矛)鑳炶〃閬�(d谩)绯荤当(t菕ng)鍜岄叺姣嶈〃閬�(d谩)绯荤当(t菕ng)銆�

8銆佽泲鐧借唱(zh矛)绲勫(xu茅)

鑰冭│鍏�(n猫i)瀹�

顥ヮ棩铔嬬櫧璩�(zh矛)绲勫(xu茅)鐨勬蹇靛強鐧�(f膩)灞曞彶銆€铔嬬櫧璩�(zh矛)绲勮〃閬�(d谩)鐨勭爺绌舵柟娉� 

鑰冭│瑕佹眰

锛�1锛夋帉鎻¤泲鐧借唱(zh矛)绲勫強铔嬬櫧璩�(zh矛)绲勫(xu茅)鐨勬蹇�锛屼簡瑙ｈ泲鐧借唱(zh矛)绲勫(xu茅)鐨勭櫦(f膩)灞曞彶銆�

锛�2锛夋帉鎻¤泲鐧借唱(zh矛)绲勮〃閬�(d谩)妯″紡鐮旂┒鐨勫熀鏈椹�锛岀悊瑙ｅ畾閲忚泲鐧借唱(zh矛)绲勭爺绌剁殑绛栫暐銆�

9銆佸熀鍥犺綁(zhu菐n)绉绘妧琛�(sh霉)鍜屽熀鍥犳墦鍚ф妧琛�(sh霉)

鑰冭│鍏�(n猫i)瀹�

顥ヮ棩顥ヨ綁(zhu菐n)鍩哄洜鍕�(d貌ng)鐗┿€€鍕�(d貌ng)鐗╄綁(zhu菐n)鍩哄洜鐨勫熀鏈師鐞嗐€€鍕�(d貌ng)鐗╄綁(zhu菐n)鍩哄洜鐨勫熀鏈柟娉�   鍩哄洜鎵撻澏鎶€琛�(sh霉)  鍩哄洜淇＞鍕�(d貌ng)鐗╃殑鎳�(y墨ng)鐢�

鑰冭│瑕佹眰

顥ワ紙1锛夌悊瑙ｈ綁(zhu菐n)鍩哄洜鍕�(d貌ng)鐗�銆佸熀鍥犲墧闄ゃ€佸熀鍥犻崶鍏ョ殑姒傚康銆�

锛�2锛夋帉鎻″嫊(d貌ng)鐗╄綁(zhu菐n)鍩哄洜鎶€琛�(sh霉)鐨勫熀鏈師鐞�锛岀悊瑙ｅ嫊(d貌ng)鐗╄綁(zhu菐n)鍩哄洜鎶€琛�(sh霉)鐨勫熀鏈柟娉�锛屾帉鎻¤綁(zhu菐n)鍩哄洜鍕�(d貌ng)鐗╃殑妾㈡脯(c猫)銆�

锛�3锛夋帉鎻″熀鍥犳墦闈剁殑姒傚康锛岀悊瑙ｅ熀鍥犳墦闈剁殑鍩烘湰鍘熺悊锛屼簡瑙ｅ熀鍥犳墦闈剁殑鍩烘湰鏂规硶銆�

10銆佸熀鍥犺ê鏂�

鑰冭│鍏�(n猫i)瀹�

鍩哄洜瑷烘柗鐨勬蹇�  鍩哄洜瑷烘柗鐨勬妧琛�(sh霉)  鍩哄洜瑷烘柗鐨勬柟娉�  閬哄偝鐥呯殑鍩哄洜瑷烘柗鍌虫煋鐥呯殑鍩哄洜瑷烘柗  鑵槫鐨勫熀鍥犺ê鏂� 鍩哄洜瑷烘柗鍦ㄦ硶閱�(y墨)瀛�(xu茅)涓婄殑鎳�(y墨ng)鐢�

鑰冭│瑕佹眰

锛�1锛夌悊瑙ｅ熀鍥犺ê鏂风殑姒傚康锛屾帉鎻″熀鍥犺ê鏂风殑鐗归粸(di菐n)銆�

锛�2锛夌悊瑙ｅ熀鍥犺ê鏂峰父鐢ㄧ殑鍒嗗瓙鐢熺墿瀛�(xu茅)鎶€琛�(sh霉)銆� 

锛�3锛変簡瑙ｅ熀鍥犺ê鏂风殑鎶€琛�(sh霉)璺窔锛屾帉鎻＄洿鎺ュ熀鍥犺ê鏂烽€斿緫鍜岄枔鎺ュ熀鍥犺ê鏂烽€斿緫鐨勪富瑕佹柟娉�銆�

锛�4锛変簡瑙ｄ富瑕佺殑閬哄偝鐥�锛岀悊瑙ｅ叾鍩哄洜瑷烘柗鐨勪富瑕佹柟娉�銆�

锛�5锛変簡瑙ｄ富瑕佺殑鍌虫煋鐥咃紝鐞嗚В鍏跺熀鍥犺ê鏂风殑涓昏鏂规硶銆�

锛�6锛変簡瑙ｅ師鐧屽熀鍥犲拰鎶戠檶鍩哄洜鐨勬娓�(c猫)鍙婂叾鍦ㄨ叓鐦よê鏂蜂腑鐨勬噳(y墨ng)鐢�銆�

锛�7锛夋帉鎻NA鎸囩磱鐨勬蹇�锛岀悊瑙NA鎸囩磱鍒嗘瀽鐨勫熀鏈祦绋�锛屼簡瑙NA鎸囩磱鍦ㄦ硶閱�(y墨)瀛�(xu茅)涓婄殑鎳�(y墨ng)鐢ㄣ€�

11銆佸熀鍥犳不鐧�

鑰冭│鍏�(n猫i)瀹�:

鍩哄洜娌荤檪鐨勬蹇�  鍩哄洜娌荤檪鐨勫熀鏈瓥鐣�  鍩哄洜杞�(zhu菐n)绉荤殑鍩烘湰鎶€琛�(sh霉)  鍩哄洜杞�(zhu菐n)绉荤殑闈剁窗(x矛)鑳�  鍩哄洜骞查爯(y霉) 

鑰冭│瑕佹眰

锛�1锛夋帉鎻″熀鍥犳不鐧傜殑姒傚康銆�

锛�2锛夌悊瑙ｅ熀鍥犳不鐧傜殑鍩烘湰绛栫暐銆�

锛�3锛夋帉鎻＄梾姣掍粙灏�(d菐o)鐨勫熀鍥犺綁(zhu菐n)绉荤郴绲�(t菕ng)锛岀悊瑙ｈ剛璩�(zh矛)楂斾粙灏�(d菐o)鐨勫熀鍥犺綁(zhu菐n)绉绘妧琛�(sh霉)锛屼簡瑙ｅ彈楂斾粙灏�(d菐o)鐨勫熀鍥犺綁(zhu菐n)绉绘妧琛�(sh霉)鍜屽熀鍥犵洿鎺ユ敞灏勬妧琛�(sh霉)銆�

锛�4锛変簡瑙ｅ熀鍥犺綁(zhu菐n)绉荤殑甯哥敤闈剁窗(x矛)鑳炪€�

锛�5锛夋帉鎻NA骞叉摼鎶€琛�(sh霉)鐨勬蹇靛拰鍘熺悊銆�

12銆佽叓鐦ゅ垎瀛愮敓鐗╁(xu茅)

鑰冭│鍏�(n猫i)瀹�:

鑵槫鐧�(f膩)鐢熺殑鍒嗗瓙姗�(j墨)鍒�  鐧屽熀鍥�  鎶戠檶鍩哄洜

鑰冭│瑕佹眰:

锛�1锛夌悊瑙ｈ叓鐦ょ櫦(f膩)鐢熺殑鍒嗗瓙姗�(j墨)鍒�銆�

锛�2锛夋帉鎻＄檶鍩哄洜鐨勬蹇碉紝鐞嗚В鍘熺檶鍩哄洜婵€娲荤殑姗�(j墨)鍒�锛屼簡瑙ｄ富瑕佺殑鐧屽熀鍥�锛岀啛鎮夌檶鍩哄洜鐨勫垎椤�銆�

锛堬紙2锛夋帉鎻℃姂鐧屽熀鍥犵殑姒傚康锛岀悊瑙ｄ富瑕佺殑鎶戠檶鍩哄洜鍙婂叾浣滅敤姗�(j墨)鍒讹紝浜嗚В鎶戠檶鍩哄洜澶辨椿鐨勬(j墨)鍒�銆�