2021骞�灞辨澅澶у(xu茅)鑰冪爺澶х侗

642-鐢熺墿鍖栧(xu茅)锛堥啱(y墨)锛�

涓€銆佽€冭│鐩殑

鐢熺墿鍖栧(xu茅)鏄敓鐗╁寲瀛�(xu茅)鑸囧垎瀛愮敓鐗╁(xu茅)灏堟キ(y猫)鍙婂叾瀹冪浉闂�(gu膩n)灏堟キ(y猫)纰╁＋鐮旂┒鐢熺殑鍏ュ(xu茅)鑰冭│绉戠洰涔嬩竴銆傜敓鐗╁寲瀛�(xu茅)鐮旂┒鐢熷叆瀛�(xu茅)鑰冭│鏄偤鎵€鎷涙敹鑸囩敓鐗╁寲瀛�(xu茅)鏈夐棞(gu膩n)灏堟キ(y猫)纰╁＋鐮旂┒鐢熻€屽(sh铆)鏂界殑鍏锋湁閬告嫈鍔熻兘鐨勬按骞宠€冭│銆�

浜�銆佽€冭│瑕佹眰

鐢熺墿鍖栧(xu茅)鑸囧垎瀛愮敓鐗╁(xu茅)鏄敓鍛界瀛�(xu茅)鐨勫熀绀�(ch菙)鐞嗚珫瑾诧紝瑕佹眰瀛�(xu茅)鐢熸瘮杓冪郴绲�(t菕ng)鍦扮悊瑙ｅ拰鎺屾彙鐢熺墿鍖栧(xu茅)鐨勫熀鏈蹇靛拰鍩烘湰鐞嗚珫锛涙帉鎻″悇椤炵敓鍖栫墿璩�(zh矛)鐨勭祼(ji茅)妲�(g貌u)銆佹€ц唱(zh矛)銆佸姛鑳藉強鍏跺悎鎴愪唬璎濆拰鍒嗚В浠ｈ瑵鐨勫熀鏈€斿緫鍜岃(di脿o)鎺ф柟娉曪紱鐞嗚В鍩哄洜琛ㄩ仈(d谩)銆佽(di脿o)鎺у拰鍩哄洜宸ョ▼鐨勫熀鏈悊璜栵紱鑳界稖鍚堥亱(y霉n)鐢ㄦ墍瀛�(xu茅)鐨勭煡璀樺垎鏋愬晱椤屽拰瑙ｆ焙鍟忛銆傝姹傝€冪敓鑳界珯鍦ㄥ(xu茅)绉戠殑鍓嶆部锛屾妸鎻″(xu茅)绉戠殑閫�(j矛n)灞�锛岄潏娲婚亱(y霉n)鐢ㄦ墍瀛�(xu茅)鐨勭敓鐗╁寲瀛�(xu茅)鐭ヨ瓨寰炲垎瀛愭按骞宠獚(r猫n)璀樺拰瑙ｉ噵鐢熷懡閬庣▼涓墍鐧�(f膩)鐢熺殑鐝�(xi脿n)璞�銆�

涓夈€佽€冭│鍏�(n猫i)瀹�

锛堜竴锛夎泲鐧借唱(zh矛)鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳�

1. 鎺屾彙铔嬬櫧璩�(zh矛)鐨勫熀鏈祫鎴愬柈浣�锛�20绋甃-伪-姘ㄥ熀閰革紝鐔熸倝姘ㄥ熀閰哥殑閫氬紡鑸囩祼(ji茅)妲�(g貌u)鐗归粸(di菐n)銆傛帉鎻℃皑鍩洪吀鐨勫叐鎬цВ闆㈠拰绱鍚告敹鎬ц唱(zh矛)銆傛帉鎻¤偨閸靛強澶氳偨閺堢殑閫ｆ帴鏂瑰紡锛屼簡瑙ｇ敓鐗╂椿鎬ц偨銆�

2. 鎺屾彙铔嬬櫧璩�(zh矛)鐨勫垎瀛愮祼(ji茅)妲�(g貌u)锛屽寘鎷偨鍠厓鍙婁竴绱�銆佷簩绱氱祼(ji茅)妲�(g貌u)锛岋紙伪-铻烘棆銆佄�-鎶樼枈銆佄�-杞�(zhu菐n)瑙掋€佺劇瑕�(gu墨)鍗锋洸锛�锛屼笁绱氥€佸洓绱氱祼(ji茅)妲�(g貌u)姒傚康鍜岀董鎸侀嵉銆傛帉鎻℃ā搴忋€佺祼(ji茅)妲�(g貌u)鍩�銆佸垎瀛愪即渚舵蹇点€�

3. 鐔熸倝铔嬬櫧璩�(zh矛)鍚勭礆绲�(ji茅)妲�(g貌u)鑸囧姛鑳介棞(gu膩n)绯�锛岀啛鎮夎绱呰泲鐧界殑鍒嗗瓙绲�(ji茅)妲�(g貌u)銆傝绱呰泲鐧界┖闁撶祼(ji茅)妲�(g貌u)鑸囬亱(y霉n)姘у姛鑳介棞(gu膩n)绯�銆傛帉鎻″崝(xi茅)鍚屾晥鎳�(y墨ng)銆佸垾妲�(g貌u)鏁堟噳(y墨ng)鐨勬蹇�銆�

4. 鎺屾彙铔嬬櫧璩�(zh矛)鍏╂€ч浕闆€€佽Κ姘磋啝楂�銆佽畩鎬�銆佺传澶栧惛鏀剁瓑鎬ц唱(zh矛)鍙婄浉闂�(gu膩n)姒傚康锛岀啛鎮夎泲鐧借唱(zh矛)鍒嗛洟绱斿寲鏂规硶鍜屼緷鎿�(j霉)鐨勬€ц唱(zh矛)銆備簡瑙ｅ鑲介張姘ㄥ熀閰告脯搴忕殑鍘熺悊銆�

5. 鐔熸倝铔嬬櫧璩�(zh矛)绲勫(xu茅)鐨勬蹇靛強鐮旂┒鏂规硶銆�

锛堜簩锛夋牳閰哥殑绲�(ji茅)妲�(g貌u)鑸囧姛鑳�

1. 鎺屾彙鏍歌嫹閰稿垎瀛愮祫鎴愬強绲�(ji茅)妲�(g貌u)锛孌NA銆丷NA绲勬垚鐨勭暟鍚�銆�

2. 鎺屾彙鏍搁吀锛圖NA銆丷NA锛夌殑涓€绱氱祼(ji茅)妲�(g貌u)锛岄€ｆ帴閸�銆�

3. 鎺屾彙DNA闆欒灪鏃嬬祼(ji茅)妲�(g貌u)妯″紡鐨勮榛�(di菐n)锛孌NA鐨勮秴铻烘棆绲�(ji茅)妲�(g貌u)鍜屽姛鑳�銆�

4. 鎺屾彙tRNA銆乵RNA銆乺RNA鐨勭祫鎴愩€佺祼(ji茅)妲�(g貌u)鐗归粸(di菐n)鍜屽姛鑳�銆�

5. 鐔熸倝浠ヤ笅姒傚康锛氳瀺瑙ｆ韩搴︺€佸鑹叉晥鎳�(y墨ng)銆丏NA璁婃€у拰寰�(f霉)鎬с€佹牳閰稿垎瀛愰洔浜�銆佹帰閲濇妧琛�(sh霉)銆�

锛堜笁锛夐叾

1. 鎺屾彙閰剁殑姒傚康锛涢叾鐨勫皥涓€鎬у強鍒嗛锛涢叾鐨勫偓鍖栨(j墨)鍒�銆傛帉鎻＄祼(ji茅)鍚堥叾銆佽紨閰惰垏杓斿熀鐨勬蹇碉紱鐔熸倝绲�(ji茅)鍚堥叾涓紨閰�銆侀噾灞洟瀛愮殑浣滅敤銆傛帉鎻℃椿鎬т腑蹇冦€佸繀闇€鍩哄湗(tu谩n)鐨勬蹇�锛�

2. 鎺屾彙閰剁殑鐗圭暟鎬э紝閰跺弽鎳�(y墨ng)鐗归粸(di菐n)銆備簡瑙ｉ叾-搴曠墿涓枔寰�(f霉)鍚堢墿瀛�(xu茅)瑾€�

3. 鎺屾彙褰遍熆閰跺偓鍖栭€熷害鐨勫洜绱�锛岀背姘忓父鏁�(sh霉)Km锛屾渶澶у弽鎳�(y墨ng)閫熷害Vmax姒傚康鍙婃剰缇�锛涙渶閬﹑H,鏈€閬╂韩搴�锛涚鐖€ф姂鍒跺姂绲�(ji茅)妲�(g貌u)浣滅敤鐗归粸(di菐n)锛涚啛鎮変笉鍚岄鍨嬪彲閫嗘姂鍒朵綔鐢ㄧ殑鍕曞姏瀛�(xu茅)鐗归粸(di菐n)銆�

4. 鎺屾彙闂�(gu膩n)閸甸叾銆佽畩妲�(g貌u)閰剁殑姒傚康銆傞叾娲绘€ц畩妲�(g貌u)瑾�(di脿o)绡€(ji茅)鐨勬(j墨)鐞�锛屽叡鍍逛慨椋剧殑姒傚康銆傜啛鎮夊崝(xi茅)鍚屾晥鎳�(y墨ng)鐨勬蹇点€傛帉鎻￠叾鍘�銆侀叾鍘熸縺娲荤殑姒傚康锛涘悓宸ラ叾鐨勬蹇碉紱鐔熸倝閰惰泲鐧藉悎鎴愯獦灏�(d菐o)鑸囬樆閬忔蹇�

5. 浜嗚В閰惰垏閱�(y墨)瀛�(xu茅)鐨勯棞(gu膩n)绯�

锛堝洓锛夌董鐢熺礌鑸囪紨鍥犲瓙

1. 鎺屾彙缍敓绱犵殑姒傚康銆備簡瑙ｇ董鐢熺礌鐨勫垎椤炲強鐩搁棞(gu膩n)鐨勭己涔忕櫏

2. 鐞嗚В缍敓绱犲湪浠ｈ瑵銆佺敓鐗╂哀鍖栧強閬哄偝淇℃伅鍌抽仦涓殑浣滅敤

3. 鎺屾彙姘存憾鎬х董鐢熺礌鐨勭敓鍖栦綔鐢紝姘存憾鎬х董鐢熺礌鐨勮紨閰跺舰寮忓強浣滅敤銆傝憠閰搞€佺董鐢熺礌B12缂轰箯鑸囨儭鎬ц钵琛€銆傛帉鎻＄董鐢熺礌C鐨勭敓鍖栦綔鐢�锛屽琛€鐥�銆�

锛堜簲锛夌硸浠ｈ瑵

1. 鎺屾彙绯栫殑鐢熺悊鍔熻兘锛屾秷鍖栧惛鏀堕亷绋�銆侀儴浣嶅拰閰堕銆�

2. 鎺屾彙绯栭叺瑙ｇ殑涓昏閬庣▼锛岄棞(gu膩n)閸甸叾锛岃(di脿o)绡€(ji茅)鏂瑰紡锛岀敓鐞嗘剰缇�銆�

3. 鎺屾彙绯栫殑鏈夋哀姘у寲鐨勪富瑕侀亷绋�锛岄棞(gu膩n)閸甸叾锛岃(di脿o)绡€(ji茅)鏂瑰紡锛岀敓鐞嗘剰缇┿€傛帉鎻′笁缇ч吀寰挵(hu谩n)鐨勯亷绋�锛岀壒榛�(di菐n)锛屾剰缇╁強瑾�(di脿o)绡€(ji茅)銆�

4. 鎺屾彙纾烽吀鎴婄硸閫斿緫姘у寲闅庢閬庣▼锛孨ADPH鍜�5-纾烽吀鏍哥硸鐢�(ch菐n)鐢熺殑鐢熺悊鎰忕京銆�

5. 鎺屾彙绯栧師鍚堟垚鍒嗚В鐨勫熀鏈亷绋�锛岄棞(gu膩n)閸甸叾锛岃(di脿o)绡€(ji茅)鏂瑰紡銆傝倢绯栧師鍜岃倽绯栧師浠ｈ瑵鐨勭暟鍚�銆�

6. 鎺屾彙绯栫暟鐢熺殑姒傚康锛屽熀鏈亷绋嬶紝鐢熺悊鎰忕京锛涗钩閰稿惊鐠�(hu谩n)鐨勬蹇点€備簡瑙ｇ硸鐣扮敓瑾�(di脿o)绡€(ji茅)鐗归粸(di菐n)銆�

7. 鎺屾彙琛€绯栨蹇�锛岃绯栫殑渚嗘簮鍘昏矾锛岃儼宄剁礌灏嶈绯栫殑瑾�(di脿o)绡€(ji茅)姗�(j墨)鐞�銆傜啛鎮夎儼楂樿绯栫礌銆佺硸鐨唱(zh矛)婵€绱犲崌楂樿绯栨(j墨)鐞�銆備簡瑙ｈ绯栨按骞崇暟甯�銆�

锛堝叚锛夎剛椤炰唬璎�

1. 鎺屾彙鑴傝偑娑堝寲鍚告敹鐨勬浠讹紝鑶芥眮閰搁菇鍙婅紨鑴傞叾鐨勪綔鐢�锛屼钩绯滃井绮掔殑褰㈡垚銆傜啛鎮夎剛椤炴秷鍖栧惛鏀堕亷绋�銆備簡瑙ｈ剛椤炵殑鍒嗛鍔熻兘銆�

2. 鎺屾彙鐢樻补涓夐叝鍚堟垚閬庣▼銆傝剛鑲嫊鍝＄殑姒傚康锛岄檺閫熼叾鍙婅(di脿o)绡€(ji茅)銆傛帉鎻＄敇娌逛唬璎濆強鑴傝偑閰肝�-姘у寲鐨勫叏閬庣▼锛岄棞(gu膩n)閸甸叾鍙婅兘閲忕敓鎴�銆�

3. 鎺屾彙閰珨鐨勬蹇�锛屽悎鎴愬強鍒╃敤鐨勯儴浣�銆侀亷绋嬪拰鐢熺悊鎰忕京銆�

4. 鎺屾彙鑴傝偑閰稿悎鎴愮殑鍘熸枡锛岄棞(gu膩n)閸甸叾鍙婅(di脿o)绡€(ji茅)銆傚繀闇€鑴傝偑閰哥殑姒傚康銆傜啛鎮夎剛鑲悎鎴愰亷绋�銆備簡瑙ｈ剛閰稿叾瀹冩哀鍖栫殑鏂瑰紡锛屼笉椋藉拰鑴傝偑閰哥殑閲嶈琛嶇敓鐗┿€�

5. 鎺屾彙纾疯剛鐨勫垎椤�銆佺敇娌圭７鑴傜殑鍚堟垚鍙婇檷瑙ｉ€斿緫銆備簡瑙ｉ灅鑴傜殑鍒嗛銆佸悎鎴愩€�

6. 鎺屾彙鑶藉浐閱囧悎鎴愪唬璎濈殑鍘熸枡銆侀棞(gu膩n)閸甸叾鍙婅(di脿o)绡€(ji茅)銆傝喗鍥洪唶鐨勮綁(zhu菐n)鍖�銆�

7. 鎺屾彙琛€婕胯剛铔嬬櫧鍒嗛鍙婄祫鎴�锛岃級鑴傝泲鐧界敓鐞嗗姛鐢�銆傚洓绋剛铔嬬櫧鐨勪唬璎濇娉併€備簡瑙ｉ珮鑴傝泲鐧借鐧ョ殑鍒嗗瀷鍙婅鑴傜暟甯�銆�

锛堜竷锛夌敓鐗╂哀鍖�

1. 鎺屾彙闆诲瓙鍌抽仦閺堢殑姒傚康锛岀祫鍒�锛屾帓鍒楅爢搴�锛屽叐姊濋浕瀛愬偝閬為張銆�

2. 鎺屾彙搴曠墿姘村钩纾烽吀鍖栬垏姘у寲纾烽吀鍖栫殑姒傚康銆�

3. 鎺屾彙ATP鍚堟垚鍋惰伅(li谩n)閮ㄤ綅銆傜啛鎮堿TP鍚堥叾绲�(ji茅)妲�(g貌u)锛孉TP鍚堟垚鍋惰伅(li谩n)姗�(j墨)鐞�銆傜啛鎮夊奖闊挎哀鍖栫７閰稿寲鐨勫洜绱�銆�

4. 鐔熸倝ATP寰挵(hu谩n)锛岄珮鑳界７閰搁嵉椤炲瀷锛岃帛瀛樺拰杞�(zhu菐n)绉�銆�

5. 鎺屾彙NADH杞�(zhu菐n)閬�(y霉n)鐨勫叐绋┛姊(j墨)鍒�銆傜啛鎮堿TP/ADP杞�(zhu菐n)閬�(y霉n)銆�

6. 鐔熸倝閬庢哀鍖栫墿閰�銆丼OD鍜屽姞鍠哀閰�銆備簡瑙ｅ叾瀹冩哀鍖栭珨绯婚叾椤�銆�

锛堝叓锛夋皑鍩洪吀浠ｈ瑵

1. 鎺屾彙姘钩琛″強蹇呴渶姘ㄥ熀閰哥殑姒傚康锛岀啛鎮夎泲鐧借唱(zh矛)鐨勭敓鐞嗗姛鑳姐€�

2. 鎺屾彙铔嬬櫧璩�(zh矛)鐨勮厫鏁椾綔鐢ㄥ強鑵愭晽鐢�(ch菐n)鐗�銆傜啛鎮夎泲鐧借唱(zh矛)娑堝寲涓悇绋叾鐨勪綔鐢ㄥ強纬-璋锋皑?锛涱媯顒(hu谩n)銆傜啛鎮夐珨鍏�(n猫i)铔嬬櫧璩�(zh矛)闄嶈В鐨勪富瑕侀€斿緫銆�

3. 鎺屾彙姘ㄥ熀閰歌劔姘ㄥ熀浣滅敤鏂瑰紡锛氳綁(zhu菐n)姘ㄥ熀锛孡-璋锋皑閰告哀鍖栬劔姘ㄥ熀锛岃伅(li谩n)鍚堣劔姘ㄥ熀锛屽熀鏈亷绋嬨€傜啛鎮壩�-閰吀浠ｈ瑵姒傛硜銆�

4. 鎺屾彙姘ㄧ殑渚嗘簮鍜屽幓璺�锛屾皑鐨勮綁(zhu菐n)閬�(y霉n)閬庣▼锛屼笝姘ㄩ吀-钁¤悇绯栧惊鐠�(hu谩n)銆傛帉鎻″翱绱犵敓鎴愰偿姘ㄩ吀寰挵(hu谩n)鐨勯亷绋�銆侀儴浣嶅強瑾�(di脿o)绡€(ji茅)銆�

5. 鎺屾彙姘ㄥ熀閰歌劔缇у熀浣滅敤锛岀敓鎴愮殑鐢熺悊娲绘€х墿璩�(zh矛)銆�

6. 鎺屾彙涓€纰冲柈浣嶇殑姒傚康銆佽級楂斿強鐢熺悊鍔熻兘銆�

7. 鎺屾彙娲绘€х敳鍩虹殑褰㈠紡銆傜啛鎮夌敳纭皑閰稿惊鐠�(hu谩n)鍜岃倢閰稿悎鎴�銆備簡瑙ｇ敱鑻笝姘ㄩ吀鍜岄叒姘ㄩ吀鐢熸垚鐨勭敓鐞嗘椿鎬х墿璩�(zh矛)銆�

锛堜節锛夋牳鑻烽吀浠ｈ瑵

1.  鎺屾彙鍢屽懁鏍歌嫹閰稿緸闋悎鎴愰€斿緫锛氭蹇�銆佸師鏂�銆侀棞(gu膩n)閸甸叾鍙婇亷绋�銆傜啛鎮夋牳鑻烽吀鐢熺墿鍔熻兘銆佸槍鍛ゆ牳鑻烽吀瑁�(b菙)鏁戝悎鎴愰€斿緫銆備簡瑙ｆ牳閰哥殑娑堝寲銆�

2. 鎺屾彙鑴哀鏍歌嫹閰哥殑鐢熸垚锛屾牳绯栨牳鑻烽吀閭勫師閰剁殑鎴愬垎銆�

3. 鎺屾彙鍢屽懁鏍歌嫹閰稿垎瑙ｄ唬璎濈祩鐢�(ch菐n)鐗�锛涚啛鎮夊槍鍛ゆ牳鑻烽吀鎶椾唬璎濈墿浣滅敤銆傜棝棰�(f膿ng)鐧ョ殑鍘熷洜鍙婃不鐧傚師鍓�銆�

4. 鎺屾彙鍢у暥鏍歌嫹閰稿緸闋悎鎴愰€斿緫锛氭蹇点€佸師鏂�銆侀棞(gu膩n)閸甸叾鍙婇亷绋�銆傛帉鎻¤劔姘ц兏鑵哄槯鍟舵牳鑻烽吀鐨勭敓鎴�銆�

5. 鐔熸倝鍢у暥鏍歌嫹閰歌(b菙)鏁戝悎鎴愰€斿緫锛屽槯鍟舵牳鑻烽吀鎶椾唬璎濈墿浣滅敤銆�

6. 鐔熸倝鏍歌嫹閰哥殑杞�(zhu菐n)璁婇棞(gu膩n)绯汇€傛牳鑻烽吀鍚堟垚瑾�(di脿o)绡€(ji茅)鐨勫熀鏈柟寮�銆�

锛堝崄锛夌墿璩�(zh矛)浠ｈ瑵鐨勮伅(li谩n)绯昏垏瑾�(di脿o)绡€(ji茅)

1. 鐔熸倝楂斿収(n猫i)鐗╄唱(zh矛)浠ｈ瑵鐨勭壒榛�(di菐n)銆�

2. 鎺屾彙绯�銆佽剛椤炪€佽泲鐧借唱(zh矛)涓夊ぇ鐗╄唱(zh矛)鍦ㄨ兘閲忎唬璎�銆佺墿璩�(zh矛)浠ｈ瑵闁撶殑鐩镐簰褰遍熆鍜屼簰鐩歌綁(zhu菐n)鍖栨(j墨)鍒�銆�

3. 鐔熸倝涓昏绲勭箶鍣ㄥ畼鐨勪唬璎濈壒榛�(di菐n)銆�

4. 鎺屾彙绱�(x矛)鑳炴按骞充唬璎濊(di脿o)绡€(ji茅)姒傚康銆傞棞(gu膩n)閸甸叾銆侀叾鐨勮畩妲�(g貌u)瑾�(di脿o)绡€(ji茅)銆侀叾鐨勫寲瀛�(xu茅)淇＞瑾�(di脿o)绡€(ji茅)姒傚康銆佺敓鐞嗘剰缇╁拰鐗归粸(di菐n)銆傜啛鎮夐椁撳拰鎳�(y墨ng)婵€鏅傛(j墨)楂旂殑鏁撮珨瑾�(di脿o)绡€(ji茅)姒傛硜銆備簡瑙ｉ叾閲忚(di脿o)绡€(ji茅)鍜屾縺绱犳按骞宠(di脿o)绡€(ji茅)姒傚康銆�

锛堝崄涓€锛夎娑茬殑鐢熺墿鍖栧(xu茅)

1. 鎺屾彙琛€婕胯泲鐧界殑鍔熻兘銆傜啛鎮夎婕胯泲鐧界殑鍒嗛鍜屼富瑕佹€ц唱(zh矛)銆�

2. 鎺屾彙绱呯窗(x矛)鑳炵硸浠ｈ瑵鐗归粸(di菐n)銆�2锛�3-浜岀７閰哥敇娌归吀鏃佽矾锛孨ADH鍜孨ADPH鐨勫姛鑳�銆�

3. 鎺屾彙琛€绱呯礌鍚堟垚鍘熸枡銆侀棞(gu膩n)閸甸叾銆佷富瑕侀亷绋�銆傜啛鎮夎绱呯礌鍚堟垚瑾�(di脿o)绡€(ji茅)锛岀櫧绱�(x矛)鑳炵殑浠ｈ瑵銆�

锛堝崄浜岋級鑲濈殑鐢熺墿鍖栧(xu茅)

1. 鎺屾彙鑲濊嚐鍦ㄧ硸銆佽剛椤炲拰铔嬬櫧璩�(zh矛)浠ｈ瑵涓噸瑕佷綔鐢ㄣ€傜啛鎮夎倽鍦ㄧ董鐢熺礌銆佹縺绱犱唬璎濅腑鐨勪綔鐢ㄣ€�

2. 鎺屾彙鐢熺墿杞�(zhu菐n)鍖栦綔鐢ㄧ殑姒傚康銆傝倽鐢熺墿杞�(zhu菐n)鍖栧弽鎳�(y墨ng)鐨勯鍨�锛屽姞鍠哀閰剁郴绲勬垚銆佷綔鐢�銆傜祼(ji茅)鍚堝弽鎳�(y墨ng)鐨勭ó椤烇紝绲�(ji茅)鍚堝熀鍦�(tu谩n)娲绘€т緵楂�銆侀叾椤炪€傜啛鎮夊奖闊跨敓鐗╄綁(zhu菐n)鍖栧洜绱�銆�

3. 鎺屾彙鑶芥眮閰稿垎椤�锛屽垵绱氳喗姹侀吀鍚堟垚锛岃喗姹侀吀鑵歌倽寰挵(hu谩n)銆備簡瑙ｈ喗姹侀吀鍔熻兘銆�

4. 鎺屾彙鑶借壊绱犵敓鎴�銆佽綁(zhu菐n)閬�(y霉n)閬庣▼銆傝喗绱呯礌鍦ㄨ倽鑷熻綁(zhu菐n)璁�銆傝喗绱呯礌鑵搁亾涓畩鍖�锛岃喗鑹茬礌鑵歌倽寰挵(hu谩n)銆傛父闆㈣喗绱呯礌鑸囩祼(ji茅)鍚堣喗绱呯礌銆傜啛鎮夐粌鐤哥殑姒傚康锛屽悇鍨嬮粌鐤哥殑鐥呭洜鍙婅嚚搴婄壒寰�銆�

锛堝崄涓夛級DNA鐨勭敓鐗╁悎鎴�

1. 鎺屾彙涓績娉曞墖銆佸熀鍥犺〃閬�(d谩)銆佸崐淇濈暀寰�(f霉)鍒剁殑姒傚康銆�

2. 鎺屾彙鍙冭垏DNA寰�(f霉)鍒剁殑涓昏鐗╄唱(zh矛)鍙婂叾浣滅敤姗�(j墨)鐞嗐€傛帉鎻NA鑱氬悎閰朵綔鐢ㄧ壒榛�(di菐n)锛屽師鏍哥敓鐗╁拰鐪熸牳鐢熺墿DNA鑱氬悎閰剁殑鐣板悓銆傛嫇瑁�(b菙)鐣版(g貌u)閰�銆佸紩鐗╅叾浣滅敤銆傜啛鎮塂NA寰�(f霉)鍒剁殑鏂瑰悜鎬�銆佷繚鐪熸€с€傜啛鎮夐€ｆ帴閰朵綔鐢ㄦ(j墨)鐞�銆�

3. 鎺屾彙DNA寰�(f霉)鍒堕亷绋嬪強鍚勯殠娈电殑鐗归粸(di菐n)銆傜绮掑拰绔矑閰舵蹇靛強浣滅敤銆傜啛鎮夊京(f霉)鍒惰捣濮嬪拰宀″磶鐗囨銆佸紩鐧�(f膩)楂斻€佽矤(f霉)瓒呰灪鏃嬫蹇�銆佸舰鎴�銆備簡瑙ｆ痪鐠�(hu谩n)寰�(f霉)鍒堕亷绋�銆�

4. 鎺屾彙绐佽畩姒傚康锛孌NA鎼嶅偡鐨勯鍨�锛屽垏闄や慨寰�(f霉)鐨勫熀鏈師鐞�锛涚啛鎮夌獊璁婄殑鎰忕京銆佸紩鐧�(f膩)鍥犵礌銆傜啛鎮夊厜淇京(f霉)銆丼OS淇京(f霉)鍙婇噸绲勪慨寰�(f霉)鐨勬蹇点€�

5. 鎺屾彙閫嗚綁(zhu菐n)閷勬蹇�銆佷綔鐢ㄩ亷绋嬨€傞€嗚綁(zhu菐n)閷勯叾浣滅敤鐗归粸(di菐n)銆傜敓鐗╁(xu茅)鎰忕京鍙婃噳(y墨ng)鐢�銆�

锛堝崄鍥涳級RNA鐨勭敓鐗╁悎鎴�

1. 鎺屾彙杞�(zhu菐n)閷勭殑姒傚康锛屼笉灏嶇ū杞�(zhu菐n)閷�銆佹ā鏉块張銆佺法纰奸張銆傚師鏍哥敓鐗㏑NA鑱氬悎閰跺叏閰�锛屾牳蹇冮叾鐨勭祫鎴愬拰浣滅敤銆傜湡鏍哥敓鐗㏑NA鑱氬悎閰剁殑涓昏椤炲瀷鍜岀敘(ch菐n)鐗�銆�

2. 鎺屾彙RNA鑱氬悎閰惰垏妯℃澘杈ㄨ獚(r猫n)绲�(ji茅)鍚�銆傛帉鎻″師鏍歌綁(zhu菐n)閷勮捣濮�銆傜啛鎮夌湡鏍歌綁(zhu菐n)閷勫洜瀛�锛岃綁(zhu菐n)閷勫墠璧峰寰�(f霉)鍚堢墿銆傜啛鎮夊欢闀疯垏鍘熸牳鍏╅杞�(zhu菐n)閷勭祩姝㈤亷绋�銆�

3. 鎺屾彙鐪熸牳鍩哄洜鐨勬柗瑁傚熀鍥�銆佸収(n猫i)鍚瓙銆佸椤瓙鐨勬蹇�銆傛帉鎻RNA銆乼RNA杞�(zhu菐n)閷勫悗鐨勫姞宸ユ柟寮�銆傜啛鎮夊収(n猫i)鍚瓙鍓帴姗�(j墨)鍒�锛宺RNA鐨勫姞宸ラ亷绋�銆�

4. 鐔熸倝鏍搁叾鐨勬蹇�锛岀祼(ji茅)妲�(g貌u)銆佷綔鐢ㄧ壒榛�(di菐n)銆�

锛堝崄浜旓級铔嬬櫧璩�(zh矛)鐨勭敓鐗╁悎鎴�

1. 鎺屾彙缈昏鐨勬蹇靛強鍙冨姞铔嬬櫧璩�(zh矛)鐢熺墿鍚堟垚鐨勭墿璩�(zh矛)锛宮RNA銆乼RNA鍙婃牳绯栭珨鐨勭祼(ji茅)妲�(g貌u)鍜屽湪缈昏涓綔鐢�锛屾皑鍩洪叞-tRNA鐨勭敓鎴�銆傜啛鎮夐伜鍌冲瘑纰肩殑鐗归粸(di菐n)銆�

2. 鎺屾彙鍘熸牳鍙婄湡鏍哥敓鐗╃炕璀殑鍩烘湰閬庣▼锛岃捣濮嬮殠娈�锛屽欢闀烽殠娈电殑涓夊€嬫椹燂細娉ㄥ唺锛屾垚鑲�锛岃綁(zhu菐n)浣�銆傜祩姝㈤殠娈�銆傜啛鎮夎捣濮嬪洜瀛�銆佸欢闀峰洜瀛愬拰閲嬫斁鍥犲瓙鐨勭ó椤炲拰浣滅敤銆傚師鏍�銆佺湡鏍哥炕璀亷绋嬬暟鍚�銆�

3. 鎺屾彙澶氳仛鏍哥硸楂旂殑姒傚康銆佹剰缇�銆傜炕璀悗鍔犲伐鐨勬蹇靛強鍔犲伐鏂瑰紡锛岀啛鎮夐珮绱氱祼(ji茅)妲�(g貌u)淇＞锛屼竴绱氱祼(ji茅)妲�(g貌u)淇＞銆傝泲鐧借唱(zh矛)鍚堟垚鍚庨澏鍚戣几閫�銆�

4. 鐔熸倝甯哥敤鎶楃敓绱犵瓑鐗╄唱(zh矛)鎶戝埗缈昏鐨勬(j墨)鐞嗐€�

锛堝崄鍏級鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺�

1. 鐔熸倝鍩哄洜琛ㄩ仈(d谩)鐨勬蹇�锛屾檪闁撱€佺┖闁撴€�锛屽熀鍥犺〃閬�(d谩)鏂瑰紡锛岀敓鐗╁(xu茅)鎰忕京銆傛帉鎻″熀鍥犺綁(zhu菐n)閷勬縺娲昏(di脿o)绡€(ji茅)鐨勫熀鏈绱�銆�

2. 鎺屾彙鍘熸牳鍩哄洜鎿嶇副瀛愮殑姒傚康銆佺祼(ji茅)妲�(g貌u)鍜屽姛鑳�锛屼钩绯栨搷绺卞瓙鐨勮矤(f霉)鎬�銆佹鎬�銆佸崝(xi茅)瑾�(di脿o)瑾�(di脿o)绡€(ji茅)銆傝壊姘ㄩ吀鎿嶇副瀛�銆傜啛鎮夐樆閬忚泲鐧界殑璨�(f霉)瑾�(di脿o)鎺э紝cAMP浠嬪皫(d菐o)鐨凜AP鐨勬瑾�(di脿o)鎺�锛岃綁(zhu菐n)閷勮“娓�銆�

3. 鎺屾彙鐪熸牳鍩哄洜瑾�(di脿o)鎺ч爢寮忎綔鐢ㄥ厓浠跺拰鍙嶅紡浣滅敤鍥犲瓙鐨勬蹇�锛岀ó椤�銆傜湡鏍歌綁(zhu菐n)閷勫洜瀛愮祼(ji茅)妲�(g貌u)锛孭IC鐨勮閰嶉亷绋�銆傜啛鎮夌湡鏍稿熀鍥犵祫绲�(ji茅)妲�(g貌u)鐗归粸(di菐n)锛岀湡鏍稿熀鍥犺〃閬�(d谩)瑾�(di脿o)鎺х壒榛�(di菐n)銆�

锛堝崄涓冿級鍩哄洜閲嶇祫鑸囧熀鍥犲伐绋�

1. 鐔熸倝鑷劧鐣屽熀鍥犺綁(zhu菐n)绉诲強閲嶇祫鐨勬柟寮�銆傜祼(ji茅)鍚�銆佽綁(zhu菐n)鍖栧強杞�(zhu菐n)灏�(d菐o)銆佽綁(zhu菐n)搴�銆佸熀鍥犻噸绲�銆�

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锛堝崄鍏級绱�(x矛)鑳炰俊鎭偝閬�

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锛堝崄涔濓級鐧屽熀鍥�銆佹姂鐧屽熀鍥犺垏鐢熼暦鍥犲瓙

1. 鎺屾彙鐧屽熀鍥犳蹇�銆傜梾姣掔檶鍩哄洜姒傚康銆佽嚧鐥呮(j墨)鐞嗐€傜窗(x矛)鑳炵檶鍩哄洜鐗归粸(di菐n)鍜屽垎椤�銆�

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锛堜簩鍗侊級鍩哄洜瑷烘柗鑸囧熀鍥犳不鐧�

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锛堜簩鍗佷竴锛夊垎瀛愮敓鐗╁(xu茅)甯哥敤鎶€琛�(sh霉)鑸囦汉椤炲熀鍥犵祫瑷�(j矛)鍔�

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2. 鎺屾彙PCR鎶€琛�(sh霉)鍘熺悊锛屾噳(y墨ng)鐢ㄣ€�

3. 鐔熸倝鏍搁吀搴忓垪鍒嗘瀽鎶€琛�(sh霉)鍘熺悊銆�

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5. 浜嗚В浜洪鍩哄洜绲勮▓(j矛)鍔冨収(n猫i)瀹广€佹剰缇�锛屽悗鍩哄洜绲勭爺绌跺収(n猫i)瀹广€傜柧鐥呯浉闂�(gu膩n)鍩哄洜鍏嬮殕銆傚叾瀹冨熀鍥犲伐绋嬫妧琛�(sh霉)銆�