瑗垮畨浜ら€氬ぇ瀛�(xu茅)

鐢熺墿鍖栧(xu茅)鍩虹(ch菙)

鍙冭€冩浉鐩細鐢熺墿鍖栧(xu茅)锛堜笂涓嬪唺(c猫)锛�锛岄珮绛夋暀鑲插嚭鐗堢ぞ锛�2017骞寸鍥涚増锛屾湵鍦ｅ簹銆佸緪闀�(zh菐ng)娉曠瓑锛�ISBN978-7-04-045798-8

瑭﹂澶х侗锛�

瑭﹂绉戠洰鐨勭洰鐨�

鑰冩煡鎳�(y墨ng)瑭﹁€呭皪(du矛)妲�(g貌u)鎴愮敓鐗╅珨鍩烘湰鐗╄唱(zh矛)鐨勭祼(ji茅)妲�(g貌u)銆佹€ц唱(zh矛)銆佸姛鑳藉強鍏跺湪鐢熷懡娲诲嫊(d貌ng)涓殑鍖栧(xu茅)璁婂寲鍜岃(di脿o)鎺ц(gu墨)寰�銆佹(j墨)鍒剁殑浜嗚В銆佹帉鎻＄▼搴�锛岀偤鐢熺墿鐩搁棞(gu膩n)灏堟キ(y猫)閬告嫈鏈夊熀绀�(ch菙)銆佹湁娼涜唱(zh矛)鐨勭爺绌剁敓銆�

鍏�(n猫i)瀹硅垏鑰冩牳鐩(bi膩o)

涓昏鍏�(n猫i)瀹瑰寘鎷硸椤�銆佽剛璩�(zh矛)銆佽泲鐧借唱(zh矛)銆侀叾銆佹牳閰�銆佺董鐢熺礌銆佹縺绱犵瓑骞鹃閲嶈鐢熺墿鍒嗗瓙鐨勭祼(ji茅)妲�(g貌u)銆佹€ц唱(zh矛)銆佸姛鑳藉強鍏朵唬璎濆拰瑾�(di脿o)鎺ч€斿緫锛屼互鍙婄敓鐗╄啘鑸囩墿璩�(zh矛)閬�(y霉n)杓�?sh霉)娣栭鑴礐(j墨)鐞�銆佺敓鐗╄兘瀛�(xu茅)鑸囩敓鐗╂哀鍖栫殑鐗归粸(di菐n)鍙婂叾鑸囩墿璩�(zh矛)浠ｈ瑵鐨勯棞(gu膩n)绯�銆�

绶掕珫

鎺屾彙鍏�(n猫i)瀹癸細鐢熺墿鍖栧(xu茅)鐨勫畾缇┿€佺爺绌跺皪(du矛)璞″拰鍏�(n猫i)瀹�锛涗簡瑙ｅ収(n猫i)瀹癸細杩戜唬鐢熷寲鐧�(f膩)灞�?f脿n)椤﹔銆�

绯栭

鎺屾彙鍏�(n猫i)瀹癸細钁¤悇绯�銆佽敆绯栥€佹穩绮夌殑绲�(ji茅)妲�(g貌u)鐗归粸(di菐n)銆佸寲瀛�(xu茅)鐗规€у拰鍔熻兘锛涢噸瑕佸柈绯�銆佸绯栥€佸绯栫殑绲�(ji茅)妲�(g貌u)鐗归粸(di菐n)鍙婂叾閼掑垾鏂规硶锛涗簡瑙ｅ収(n猫i)瀹癸細绯栬泲鐧�銆佺硸鑳鸿仛绯栧強铔嬬櫧鑱氱硸鐨勭祼(ji茅)妲�(g貌u)鍙婂垎椤�銆�

鑴傝唱(zh矛)

鎺屾彙鍏�(n猫i)瀹癸細鑴傝偑閰�銆佺敇娌逛笁閰�銆佺敇娌圭７鑴�銆佽喗鍥洪唶鐨勭悊鍖栨€ц唱(zh矛)鍜岀敓鐞嗕綔鐢�锛涜剛椤炵殑鍒嗛鍜屽叡鎬�锛岃剛璩�(zh矛)閬庢哀鍖栦綔鐢紝鑴傝泲鐧界殑鍒嗛鍙婄壒鎬�锛涗簡瑙ｅ収(n猫i)瀹癸細绯栬剛銆佽悳鍙婂浐閱囪鐢熺墿銆�

姘ㄥ熀閰稿強铔嬬櫧璩�(zh矛)

鎺屾彙鍏�(n猫i)瀹癸細姘ㄥ熀閰哥殑鍒嗛銆侀吀鍫挎€ц唱(zh矛)鍙婂寲瀛�(xu茅)鍙嶆噳(y墨ng)锛涙皑鍩洪吀鐨勬按瑙ｅ強鍏夎瓬鐗规€э紱鑲界殑鐞嗗寲鎬ц唱(zh矛)锛涜泲鐧借唱(zh矛)涓€绱�(j铆)绲�(ji茅)妲�(g貌u)鐨勬脯(c猫)瀹�锛涜泲鐧借唱(zh矛)浜岀礆(j铆)绲�(ji茅)妲�(g貌u)銆佽秴浜岀礆(j铆)绲�(ji茅)妲�(g貌u)銆佺祼(ji茅)妲�(g貌u)鍩熷強涓夌礆(j铆)绲�(ji茅)妲�(g貌u)鐗归粸(di菐n)锛涜绱呰泲鐧界祼(ji茅)妲�(g貌u)鐗归粸(di菐n)鑸囧垾妲�(g貌u)鏁堟噳(y墨ng)锛涘嚌鑶犻亷婵炬硶鍜�SDS鈥擯AGE娉曟脯(c猫)瀹氬垎瀛愰噺锛涜泲鐧借唱(zh矛)鍒嗛洟绱斿寲鐨勪竴鑸師鍓囧強甯哥敤鏂规硶锛涢叞鑳哄钩闈㈣垏浜岄潰瑙�锛岃泲鐧借唱(zh矛)璁婃€у強鍒ユ(g貌u)鏁堟噳(y墨ng)锛岃啝鍘熻泲鐧藉拰鐞冪媭铔嬬櫧鐨勭祼(ji茅)妲�(g貌u)锛涙哀绲�(ji茅)鍚堟洸绶�锛屾尝鐖炬晥鎳�(y墨ng)鍜�BPG鏁堟噳(y墨ng)锛岃倢绱呰泲鐧界祼(ji茅)妲�(g貌u)鐗归粸(di菐n)锛涜泲鐧借唱(zh矛)鐨勮啝楂旀€ц唱(zh矛)鑸囨矇娣€锛屽惈閲忚垏绱斿害閼掑畾锛涗簡瑙ｅ収(n猫i)瀹癸細姘ㄥ熀閰告贩鍚堢墿鐨勫垎鏋愬垎闆紱铔嬬櫧璩�(zh矛)鐨勫垎椤炲強鐢熺墿瀛�(xu茅)鍔熻兘锛涜啘铔嬬櫧绲�(ji茅)妲�(g貌u)锛涜绱呰泲鐧藉垎瀛愮梾锛屽厤鐤悆铔嬬櫧锛岃倢鐞冭倢鍕�(d貌ng)铔嬬櫧绲�(ji茅)妲�(g貌u)鑸囧姛鑳介棞(gu膩n)绯�锛涘寲瀛�(xu茅)鍒嗘瀽娉曞強娌夐檷鍒嗘瀽娉曪紱

閰�

鎺屾彙鍏�(n猫i)瀹癸細閰剁殑鍦�(gu贸)闅涚郴绲�(t菕ng)鍛藉悕娉曞強鍒嗛锛岄叾鐨勫皥涓€鎬�锛涘簳鐗╂績搴﹀皪(du矛)閰朵績鍙嶆噳(y墨ng)閫熺巼鐨勫奖闊�锛岄叾鐨勬姂鍒朵綔鐢�锛涚背姘忔柟绋嬪強闆欏€掓暩(sh霉)娉曠瓑锛涢叾鐨勬椿鎬ч儴浣嶅拰瑾�(di脿o)绡€(ji茅)姗�(j墨)鍒�锛涢叾鍌寲浣滅敤鐗归粸(di菐n)锛屾椿鍔涙脯(c猫)瀹氬拰鍒嗛洟绱斿寲锛涙韩搴︺€�pH鍙婃縺娲诲姂灏�(du矛)閰朵績鍙嶆噳(y墨ng)鐨勫奖闊�锛涘奖闊块叾鍌寲鏁堢巼鐨勫洜绱犲強鍚屽伐閰�锛涗簡瑙ｅ収(n猫i)瀹癸細鏍搁叾銆佹姉楂旈叾銆侀叾宸ョ▼锛涢叾淇冨弽鎳�(y墨ng)鐨勫寲瀛�(xu茅)鍕�(d貌ng)鍔涘(xu茅)鍩虹(ch菙)锛涢叾鍌寲鍙嶆噳(y墨ng)鐨勭崹(d煤)鐗规€ц唱(zh矛)锛�

缍敓绱犺垏杓旈叾

鎺屾彙鍏�(n猫i)瀹癸細鍚勯缍敓绱犵殑鐢熺悊鍔熻兘锛涚董鐢熺礌鐨勪締(l谩i)婧愬拰杞�(zhu菐n)鍖栵紱浜嗚В鍏�(n猫i)瀹癸細缍敓绱犵殑绲�(ji茅)妲�(g貌u)銆�

鏍搁吀

鎺屾彙鍏�(n猫i)瀹癸細鏍搁吀鐨勭ó椤炲拰鍒嗗竷锛�DNA鍜�RNA鐨勫叡鍍�(ji脿)绲�(ji茅)妲�(g貌u)鍙婇珮绱�(j铆)绲�(ji茅)妲�(g貌u)锛涙牳閰哥殑姘磋В锛岃畩鎬�銆佸京(f霉)鎬у強鍏剁壒榛�(di菐n)锛涘垎鏋�DNA鍜�RNA涓昏鐨勬脯(c猫)瀹氭柟娉曞強鍘熺悊锛涙牳閰哥殑鐢熺墿瀛�(xu茅)鍔熻兘锛涙牳鑻烽吀鐨勭祫鎴愬強绲�(ji茅)妲�(g貌u)锛涙牳閰哥殑閰稿牽鎬ц唱(zh矛)鍙婄传澶栧惛鏀�锛涙牳閰稿父鐢ㄧ殑鐮旂┒鏂规硶鍙婂師鐞嗐€�crispr-cas9鎶€琛�(sh霉)绛�锛涗簡瑙ｅ収(n猫i)瀹癸細鏍搁吀鐨勭爺绌剁啊(ji菐n)鍙�锛涙牳閰哥殑闆滀氦绛�锛�

婵€绱�

鎺屾彙鍏�(n猫i)瀹癸細婵€绱犵殑浣滅敤姗�(j墨)鐞嗗拰鍒嗘硨瑾�(di脿o)绡€(ji茅)锛涘咕绋噸瑕佹縺绱犵殑浣滅敤姗�(j墨)鍒跺強鐢熺悊浣滅敤锛涗簡瑙ｅ収(n猫i)瀹癸細婵€绱犵殑鍚堟垚鑸囧垎娉�銆�

鐢熺墿鑶�

鎺屾彙鍏�(n猫i)瀹癸細鐢熺墿鑶滅殑绲勬垚鍜屾€ц唱(zh矛)锛涚敓鐗╄啘鐨勪富瑕佺壒寰佸強鍒嗗瓙绲�(ji茅)妲�(g貌u)妯″瀷锛涘皬鍒嗗瓙鐗╄唱(zh矛)鐨勯亱(y霉n)閫侊紱鐢熺墿澶у垎瀛愮殑璺ㄨ啘閬�(y霉n)閫佺瓑锛�

浠ｈ瑵绺借珫

鎺屾彙鍏�(n猫i)瀹癸細鐗╄唱(zh矛)浠ｈ瑵銆佽兘閲忎唬璎濆拰涓枔浠ｈ瑵锛涗唬璎濈爺绌剁殑灏�(du矛)璞″拰鏂规硶绛�锛涗簡瑙ｅ収(n猫i)瀹癸細浠ｈ瑵鍕�(d貌ng)鎱�(t脿i)鍜岀敓鍛界櫦(f膩)灞�锛�

鐢熺墿鑳藉(xu茅)

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绯栦唬璎�

鎺屾彙鍏�(n猫i)瀹癸細绯栭叺瑙ｇ殑鍙嶆噳(y墨ng)姗�(j墨)鍒�锛涙妾吀寰挵(hu谩n)閫斿緫涓殑鍚勬閰朵績鍙嶆噳(y墨ng)鍙婂叾浣滅敤鐗归粸(di菐n)锛涚７閰告垔绯栭€斿緫鐨勭壒榛�(di菐n)鍙婄敓鐗╁(xu茅)鎰忕京锛涚硸鍘熺殑鍚堟垚鑸囧垎瑙ｏ紱绯栦唬璎濈殑瑾�(di脿o)鎺�锛涙妾吀寰挵(hu谩n)鐨勮(di脿o)鎺�锛涚硸浠ｈ瑵鐨勫叾浠栭€斿緫锛涚硸鍘熶唬璎濈殑瑾�(di脿o)鎺х瓑锛涗簡瑙ｅ収(n猫i)瀹癸細鍏朵粬鍏⒊绯栭€�(j矛n)鍏ョ硸閰佃В鐨勯€斿緫锛涙妾吀寰挵(hu谩n)鐨勭櫦(f膩)灞曟鍙�锛涗箼閱涢吀閫斿緫锛涚硸鍘熺殑鐢熺墿瀛�(xu茅)鎰忕京绛�锛�

鐢熺墿姘у寲

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鍏夊悎浣滅敤

鎺屾彙鍏�(n猫i)瀹癸細鍏夊悎纾烽吀鍖栦綔鐢�锛涘厜鍙嶆噳(y墨ng)銆佹殫鍙嶆噳(y墨ng)绛�锛涗簡瑙ｅ収(n猫i)瀹癸細鍏夊懠鍚稿拰C4閫斿緫绛�

鑴備唬璎�

鎺屾彙鍏�(n猫i)瀹癸細鑴傝偑閰�銆佺敇娌逛笁閰殑鍒嗚В锛涜剛椤炵殑鐢熺墿鍚堟垚锛涜喗鍥洪唶浠ｈ瑵锛岃剛椤炰唬璎濈殑瑾�(di脿o)鎺�锛涜剛鑲帛瀛樼瓑锛涗簡瑙ｅ収(n猫i)瀹癸細纾疯剛銆侀灅鑴�銆佺斁閱囩殑浠ｈ瑵绛�

姘ㄥ熀閰歌泲鐧借唱(zh矛)浠ｈ瑵

鎺屾彙鍏�(n猫i)瀹癸細姘ㄥ熀閰哥殑鑴皑鍩哄拰杞�(zhu菐n)姘ㄥ熀浣滅敤锛涜剛鑲棌鍜岃姵棣欐棌姘ㄥ熀閰哥殑鐢熺墿鍚堟垚锛涜泲鐧借唱(zh矛)鍚堟垚鐨勬(j墨)鐞嗭紱鐢熺硸鍜岀敓閰皑鍩洪吀锛涙皑鍩洪吀鐨勮劔缇у熀浣滅敤锛涙皑鍩洪吀鐢熺墿鍚堟垚鐨勮(di脿o)绡€(ji茅)锛涢伜鍌冲瘑纰煎柈浣嶅拰鍩烘湰鐗规€х瓑锛涗簡瑙ｅ収(n猫i)瀹癸細姘ㄥ熀姘殑鎺掓硠锛涘咕绋噸瑕佽鐢熺墿鐨勭敓鐗╁悎鎴愶紱澶氳偨鍚堟垚鍚庣殑瀹氬悜杓搁€佽垏杞�(zhu菐n)璀悗鍔犲伐绛�锛�

鏍搁吀浠ｈ瑵

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绱�(x矛)鑳炰唬璎濊垏鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺�

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鍏朵粬鐢熺墿鍖栧(xu茅)鐩搁棞(gu膩n)鍏�(n猫i)瀹�銆�