|
鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�(gu贸)400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ(xu茅)鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗�锛岄儴鍒嗗(xu茅)鏍℃洿鏂拌嚦2012骞达紝2013骞�锛涘潎鎻愪緵鏀惰不(f猫i)涓嬭級銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛�(di菐n)鎿娾€�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛�(di菐n)鎿娾€�鑰冨崥瑭﹀嵎搴�(k霉)鈥� 涓嬭級
澶ч€ｇ悊宸ュぇ瀛�(xu茅) 2018 骞寸ⅸ澹爺绌剁敓鍏ュ(xu茅)鑰冭│澶х侗
绉戠洰浠ｇ⒓锛�631 绉戠洰鍚嶇ū(ch膿ng)锛氬垎瀛愮敓鐗╁(xu茅)
瑭﹂鍒嗙偤瀹㈣椤屽瀷鍜屼富瑙€椤屽瀷锛屽叾涓瑙€椤屽瀷锛堝～绌洪銆侀伕鎿囬銆佸垽鏂烽锛夊崰 40%锛屼富瑙€椤�
鍨嬶紙姒傚康椤�銆佸晱(w猫n)绛旈銆佺稖鍚堝垎鏋愰锛夊崰 60%銆傚叿楂斿京(f霉)缈�(x铆)澶х侗濡備笅锛�
1.鏍搁吀鑸囨煋鑹查珨鐨勭祼(ji茅)妲�(g貌u)锛欴NA 鐨勯洐铻烘棆绲�(ji茅)妲�(g貌u)鑸� DNA 鐨勫姛鑳藉拰寰�(f霉)鍒朵箣闁撶殑闂�(gu膩n)绯�锛汻NA 鐨勭祼(ji茅)妲�(g貌u)鑸�
鏍搁叾锛涙煋鑹查珨鐨勭祼(ji茅)妲�(g貌u)鑸囧京(f霉)鍒朵腑鎵€瑕佽В姹虹殑鍟�(w猫n)椤岋紱鏌撹壊楂斿熀鏈祼(ji茅)妲�(g貌u)锛屾煋鑹查珨绲�(ji茅)妲�(g貌u)鐨勫嫊(d貌ng)鎱�(t脿i)鎬у強鍏剁敓鐗�
瀛�(xu茅)鎰忕京锛屾煋鑹查珨绲�(ji茅)妲�(g貌u)鍕�(d貌ng)鎱�(t脿i)鎬ф(j墨)鍒�銆�
2. DNA 寰�(f霉)鍒讹細鑱氬悎閰剁殑绲�(ji茅)妲�(g貌u)鑸囧偓鍖�锛汥NA 寰�(f霉)鍒剁殑閬�(gu貌)绋嬶紱寰�(f霉)鍒剁殑姗�(j墨)鍒�锛� DNA 寰�(f霉)鍒惰捣濮嬬殑瑾�(di脿o)鎺э紱
DNA 寰�(f霉)鍒剁殑绲�(ji茅)鏉�
3.DNA 绐佽畩鑸囦慨寰�(f霉)锛欴NA 寰�(f霉)鍒堕尟(cu貌)瑾ょ殑鍘熷洜锛岄牷鐜�锛岄尟(cu貌)閰嶄慨寰�(f霉)姗�(j墨)鍒讹紱DNA 鎼嶅偡锛涙悕鍌蜂慨寰�(f霉)姗�(j墨)鍒�
4.DNA 閲嶇祫锛氬悓婧愰噸绲�锛涗綅榛�(di菐n)鐗圭暟鎬ч噸绲勮垏 DNA 杞�(zhu菐n)搴�
5.杞�(zhu菐n)閷勶細RNA 鑱氬悎閰剁殑绋(l猫i)鍜岀壒寰侊紝RNA 鑱氬悎閰跺偓鍖栫殑杞�(zhu菐n)閷勬椹�锛岃綁(zhu菐n)閷勫京(f霉)鍚堢墿鍦ㄨ綁(zhu菐n)閷勯亷(gu貌)绋嬩腑
鐨勭祼(ji茅)妲�(g貌u)鏀硅畩锛涘師鏍歌綁(zhu菐n)閷勭殑閬�(gu貌)绋�锛涚湡鏍歌綁(zhu菐n)閷勯亷(gu貌)绋�锛涘姞宸�锛涚湡鏍� RNA 鑱氬悎閰� I 鍜� III 杞�(zhu菐n)閷� rRNA 鍜� tRNA锛�
灏� RNA 鐨勬(j墨)鍒�
6.RNA 鍓帴锛氫笉鍚岄(l猫i)鍨嬪収(n猫i)鍚瓙鍒嗗竷鍜� RNA 鍓帴鐨勫寲瀛�(xu茅)鎬ц唱(zh矛)锛� I 鍨嬪拰 II 鍨嬪収(n猫i)鍚瓙鏍搁叾鐨勫壀鎺�
姗�(j墨)鍒讹紱鍓帴楂旂殑绲勫垎锛坰nRNPs锛�锛涘壀鎺ラ珨鐨勭祫瑁�銆佸偓鍖栨(j墨)鍒讹紱鍙畩鍓帴锛汻NA 绶ㄨ集銆�
7.缈昏鑸囬伜鍌冲瘑纰硷細mRNA 鐨勫姛鑳�锛泃RNA 鐨勫姛鑳�锛岀祼(ji茅)妲�(g貌u)锛屼互鍙婃皑鍩洪吀瑁濊級閬�(gu貌)绋�锛涙牳绯栭珨锛涚炕
璀殑閬�(gu貌)绋嬶紱閬哄偝瀵嗙⒓銆�
8.鍘熸牳瑾�(di脿o)鎺э細瑾�(di脿o)鎺х殑鍩烘湰鍘熷墖锛� Lac 鎿嶇副瀛�锛涚暟妲�(g貌u)瑾�(di脿o)鎺�锛汿rp 鎿嶇副瀛愶紱鍣弻楂旀憾婧愯垏瑁傝В
瑾�(di脿o)鎺�锛涚窗(x矛)鑿岀殑杞�(zhu菐n)閷勮捣濮嬪悗瑾�(di脿o)鎺�銆�
9.鐪熸牳瑾�(di脿o)鎺э細鐪熸牳淇濆畧鐨勮(di脿o)鎺ф(j墨)鍒讹紱DNA 绲�(ji茅)鍚堝拰婵€娲荤祼(ji茅)妲�(g貌u)鍩熺殑鐗归粸(di菐n)锛涜綁(zhu菐n)閷勫洜瀛�锛涙煋鑹查珨淇＞锛�
閬�(yu菐n)璺濅綔鐢ㄥ厓浠�銆�
10.瑾�(di脿o)鎺� RNAs锛氱窗(x矛)鑿屼腑 RNA 浠嬪皫(d菐o)鐨勮(di脿o)鎺�锛屽寘鎷� sRNA銆佹牳绯栭枊(k膩i)闂�(gu膩n)銆丆rsipr 绛�锛涚湡鏍哥敓鐗� RNA
瑾�(di脿o)鎺э紝鍖呮嫭 RNA 骞叉摼锛涘皬骞叉摼 RNA锛坰iRNA锛夎垏寰皬 RNA锛坢iRNA锛夌殑鍔犲伐鑸囧姛鑳斤紱闀�(zh菐ng)閺堥潪绶ㄧ⒓ RNA锛�
鏌撹壊楂斿け娲荤瓑銆�
11.鍒嗗瓙鐢熺墿瀛�(xu茅)鎶€琛�(sh霉)锛氭牳閰告妧琛�(sh霉)锛屽寘鎷浕娉�锛岄叾鍒�锛岄洔浜わ紙Southern锛�锛孭CR 鎶€琛�(sh霉)鐨勫師鐞�銆侀亷(gu貌)
绋嬪拰鎳�(y墨ng)鐢�锛涘厠闅嗘妧琛�(sh霉)锛屽寘鎷厠闅嗚級楂旇垏鍏嬮殕鎶€琛�(sh霉)锛屽熀鍥犵祫鍜� cDNA 鏂囧韩(k霉)寤虹珛锛涙脯(c猫)搴忥紱鍩哄洜琛ㄩ仈(d谩)鍙�
琛ㄩ仈(d谩)鍒嗘瀽锛涜泲鐧借唱(zh矛)绱斿寲鎶€琛�(sh霉)锛涜泲鐧界祫瀛�(xu茅)锛涜泲鐧借唱(zh矛)鑸囨牳閰哥浉浜掍綔鐢ㄧ殑鐮旂┒锛涘ぇ鍒嗗瓙绲�(ji茅)妲�(g貌u)鐨勭爺绌舵柟娉�锛�
杞�(zhu菐n)鍩哄洜鏂规硶锛涘熀鍥犵法杓妧琛�(sh霉)绛�銆�
鍏嶈铂(z茅)鑱叉槑锛氭湰鏂囩郴杞�(zhu菐n)杓夎嚜缍�(w菐ng)绲�(lu貌)锛屽鏈変镜鐘�锛岃珛(q菒ng)鑱�(li谩n)绯绘垜鍊戠珛鍗冲埅闄�锛屽彟锛氭湰鏂囧儏浠ｈ〃浣滆€呭€�(g猫)浜鸿榛�(di菐n)锛岃垏鏈恫(w菐ng)绔欑劇(w煤)闂�(gu膩n)銆傚叾鍘熷壍(chu脿ng)鎬т互鍙婃枃涓櫝杩版枃瀛楀拰鍏�(n猫i)瀹规湭缍�(j墨ng)鏈珯璀夊(sh铆)锛屽皪(du矛)鏈枃浠ュ強鍏朵腑鍏ㄩ儴鎴栬€呴儴鍒嗗収(n猫i)瀹广€佹枃瀛楃殑鐪熷(sh铆)鎬�銆佸畬鏁存€�銆佸強鏅�(sh铆)鎬ф湰绔欎笉浣滀换浣曚繚璀夋垨鎵胯锛岃珛(q菒ng)璁€鑰呭儏浣滃弮鑰�锛屽苟璜�(q菒ng)鑷鏍稿(sh铆)鐩搁棞(gu膩n)鍏�(n猫i)瀹�銆�
|
鏂囩珷鎼滅储
鎮ㄧ従(xi脿n)鍦ㄧ殑浣嶇疆锛� 
