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鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗�锛岄儴鍒嗗鏍℃洿鏂拌嚦2012骞达紝2013骞�锛涘潎鎻愪緵鏀惰不涓嬭級銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛炴搳鈥�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛炴搳鈥�鑰冨崥瑭﹀嵎搴�鈥� 涓嬭級
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鑰冭│绉戠洰浠ｇ⒓锛歔 ] 鑰冭│绉戠洰鍚嶇ū锛氬垎瀛愮敓鐗╁
涓€銆佽│鍗风祼(ji茅)妲�(g貌u)
1) 瑭﹀嵎鎴愮妇鍙婅€冭│鏅傞枔
鏈│鍗锋豢鍒嗙偤 100 鍒�锛岃€冭│鏅傞枔鐐� 180 鍒嗛悩銆�
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3)瑭﹀嵎鍏�(n猫i)瀹圭祼(ji茅)妲�(g貌u)
鍒嗗瓙鐢熺墿瀛� 100%
4)椤屽瀷绲�(ji茅)妲�(g貌u)
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浜�銆佽€冭│鍏�(n猫i)瀹硅垏鑰冭│瑕佹眰
1銆佸父鐢ㄥ垎瀛愮敓鐗╁鎶€琛�(sh霉)鐨勫師鐞嗗強鍏舵噳(y墨ng)鐢�
鑰冭│鍏�(n猫i)瀹�
鍒嗗瓙闆滀氦鑸囧嵃璺℃妧琛�(sh霉)锛汸CR 鎶€琛�(sh霉)鐨勫師鐞嗚垏鎳�(y墨ng)鐢�锛涘熀鍥犳枃搴�锛涚敓鐗╄姱鐗囨妧琛�(sh霉)锛�
鐢熺墿澶у垎瀛愮浉浜掍綔鐢ㄧ爺绌舵妧琛�(sh霉)
鑰冭│瑕佹眰
锛�1锛夋帉鎻″垎瀛愰洔浜よ垏鍗拌贰鎶€琛�(sh霉)鐨勫師鐞�銆侀鍒ュ強鎳�(y墨ng)鐢�
锛�2锛夋帉鎻� PCR 鐨勫伐浣滃師鐞�銆佷富瑕佺敤閫�
锛�3锛夌悊瑙ｅ咕绋噸瑕佺殑 PCR 琛嶇敓鎶€琛�(sh霉)
锛�4锛夌悊瑙ｅ熀鍥犵祫 DNA 鏂囧韩鍜� cDNA 鏂囧韩
锛�5锛夋帉鎻″熀鍥犺姱鐗囧拰铔嬬櫧璩�(zh矛)鑺墖鐨勫師鐞嗗拰涓昏鎳�(y墨ng)鐢�
锛�6锛夌悊瑙ｈ泲鐧借唱(zh矛)鐩镐簰浣滅敤鐮旂┒鎶€琛�(sh霉)
锛�7锛夌啛鎮� DNA-铔嬬櫧璩�(zh矛)鐩镐簰浣滅敤鍒嗗瓙鍒嗘瀽鎶€琛�(sh霉)
2銆丏NA 閲嶇祫鍜岄噸绲� DNA 鎶€琛�(sh霉)
鑰冭│鍏�(n猫i)瀹�
鑷劧鐣� DNA 閲嶇祫鍜屽熀鍥犺綁(zhu菐n)绉�锛涢噸绲� DNA 鎶€琛�(sh霉)锛涢噸绲� DNA 鎶€琛�(sh霉)鍦ㄩ啱(y墨)瀛镐腑鐨勬噳(y墨ng)鐢�
鑰冭│瑕佹眰
锛�1锛夌悊瑙� DNA 閲嶇祫鐨勫熀鏈柟寮�
锛�2锛夌悊瑙ｉ噸绲� DNA 鎶€琛�(sh霉)涓父鐢ㄧ殑宸ュ叿閰跺拰甯哥敤鐨勮級楂�
锛�3锛夋帉鎻￠噸绲� DNA 鎶€琛�(sh霉)鍩烘湰鍘熺悊鍙婃搷浣滄椹�
锛�4锛夌啛鎮夐噸绲� DNA 鎶€琛�(sh霉)寤ｆ硾鎳�(y墨ng)鍦ㄩ啱(y墨)瀛镐腑鐨勬噳(y墨ng)鐢�
3銆佸熀鍥犵祼(ji茅)妲�(g貌u)鑸囧姛鑳藉垎鏋愭妧琛�(sh霉)
鑰冭│鍏�(n猫i)瀹�
鍩哄洜绲�(ji茅)妲�(g貌u)鍒嗘瀽鎶€琛�(sh霉)锛涘熀鍥犺〃閬旂敘(ch菐n)鐗╁垎鏋愭妧琛�(sh霉)锛涘熀鍥犵殑鐢熺墿瀛稿姛鑳介憭瀹氭妧琛�(sh霉)
鑰冭│瑕佹眰
锛�1锛夋帉鎻″熀鍥犱竴绱氱祼(ji茅)妲�(g貌u)瑙ｆ瀽鎶€琛�(sh霉)
锛�2锛夌悊瑙ｅ熀鍥犺綁(zhu菐n)閷勮捣榛炲垎鏋愭妧琛�(sh霉)鍜屽熀鍥犳嫹璨濇暩(sh霉)鍒嗘瀽鎶€琛�(sh霉)
锛�3锛夌啛鎮夊熀鍥犲暉鍕曞瓙绲�(ji茅)妲�(g貌u)鍒嗘瀽鎶€琛�(sh霉)鍜屽熀鍥犵法纰煎簭鍒楀垎鏋愭妧琛�(sh霉)
锛�4锛夋帉鎻″湪杞�(zhu菐n)閷勬按骞冲垎鏋愬熀鍥犺〃閬旂殑鎶€琛�(sh霉)
锛�5锛夋帉鎻＄炕璀按骞冲垎鏋愬熀鍥犺〃閬旂殑鎶€琛�(sh霉)
锛�6锛夌悊瑙ｇ敤鍔熻兘鐛插緱绛栫暐鍜岀敤鍔熻兘澶辨椿绛栫暐閼掑畾鍩哄洜鍔熻兘
锛�7锛夌啛鎮夌敤闅ㄦ绐佽畩绡╅伕绛栫暐閼掑畾鍩哄洜鍔熻兘
3銆佺檶鍩哄洜銆佽叓鐦ゆ姂鍒跺熀鍥犺偛鐢熼暦鍥犲瓙
鑰冭│鍏�(n猫i)瀹�
鐧屽熀鍥�锛涜叓鐦ゆ姂鍒跺熀鍥�锛涚敓闀峰洜瀛�
鑰冭│瑕佹眰
锛�1锛夋帉鎻＄檶鍩哄洜鐨勫熀鏈蹇靛強鐧屽熀鍥犳椿鍖栫殑姗熷埗
锛�2锛夋潕闋″師鐧屽熀鍥犵殑鐢�(ch菐n)鐗╄垏鍔熻兘
锛�3锛夌悊瑙ｈ叓鐦ゆ姂鍒跺熀鍥犵殑鐧�(f膩)鐝�(xi脿n)鍜屽姛鑳�
锛�4锛夌悊瑙ｈ叓鐦ゆ姂鍒跺熀鍥犺垏鐤剧梾鐨勯棞(gu膩n)绯�
锛�5锛夌啛鎮夌檶鍩哄洜鑸囪叓鐦ゆ姂鍒跺熀鍥犲湪鑵槫鐧�(f膩)鐢熶腑鐨勪綔鐢ㄧ壒榛�
锛�6锛夌悊瑙ｇ敓闀峰洜瀛愮殑鍒嗛鍜屽姛鑳�
锛�7锛夌啛鎮夌敓闀峰洜瀛愪綔鐢ㄦ鍒跺強鑸囩柧鐥呯殑闂�(gu膩n)绯�
4銆佺柧鐥呯浉闂�(gu膩n)鍩哄洜鐨勯憭瀹氳垏鍩哄洜鍔熻兘鐮旂┒
鑰冭│鍏�(n猫i)瀹�
閼掑畾鐤剧梾鐩搁棞(gu膩n)鍩哄洜鐨勫師鍓�锛涚柧鐥呯浉闂�(gu膩n)鍩哄洜鍏嬮殕鐨勭瓥鐣ュ拰鏂规硶锛涚柧鐥呯浉闂�(gu膩n)鍩哄洜
鐨勫姛鑳界爺绌�锛�
鑰冭│瑕佹眰
锛�1锛夌啛鎮夐憭瀹氱柧鐥呯浉闂�(gu膩n)鍩哄洜鐨勫師鍓�
锛�2锛夌悊瑙ｄ笉渚濊炒鏌撹壊楂斿畾浣嶇殑鐤剧梾鐩搁棞(gu膩n)鍩哄洜鍏嬮殕绛栫暐
锛�3锛夋帉鎻″畾浣嶅厠闅嗙殑鍘熺悊
锛�4锛夌啛鎮夊叏鍩哄洜绲勯棞(gu膩n)鑱�(li谩n)鍒嗘瀽鍜屽叏澶栭’瀛愭脯搴�
锛�5锛夌啛鎮夌敓鐗╀俊鎭暩(sh霉)鎿�(j霉)搴�
锛�6锛夋帉鎻″熀鍥犳瘮灏嶅強鍔熻兘瑭噵
锛�7锛夌啛鎮夊埄鐢ㄥ伐绋嬬窗鑳炲拰鐢熺墿澶у垎瀛愰枔鐨勭浉浜掍綔鐢ㄧ爺绌跺熀鍥犲姛鑳�
锛�8锛夋帉鎻″埄鐢ㄥ熀鍥犱慨椋惧嫊鐗╂暣楂旂爺绌跺熀鍥犲姛鑳�
5銆佸熀鍥犺ê鏂峰拰鍩哄洜娌荤檪
鑰冭│鍏�(n猫i)瀹�
鍩哄洜瑷烘柗锛涘熀鍥犳不鐧�
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｅ熀鍥犺ê鏂风殑涓昏灏嶈薄鍜屾ǎ鍝佷締婧�
锛�2锛夋帉鎻″熀鍥犺ê鏂锋妧琛�(sh霉)鍙婂叾閱�(y墨)瀛告噳(y墨ng)鐢�
锛�3锛夋帉鎻″熀鍥犳不鐧傜殑鍩烘湰绛栫暐鍜屽熀鏈▼搴�
锛�4锛夌啛鎮夊熀鍥犳不鐧傜殑鑷ㄥ簥鎳�(y墨ng)鐢ㄧ従(xi脿n)鐙€
5銆佺祫瀛歌垏閱�(y墨)瀛�
鑰冭│鍏�(n猫i)瀹�
鍩哄洜绲勫锛涜綁(zhu菐n)閷勭祫瀛�锛涜泲鐧借唱(zh矛)绲勫锛涗唬璎濈祫瀛�锛涘叾浠栫祫瀛�锛涚祫瀛稿湪閱�(y墨)瀛镐笂鐨�
鎳�(y墨ng)鐢�
鑰冭│瑕佹眰
锛�1锛夋帉鎻″熀鍥犵祫瀛稿寘鍚祼(ji茅)妲�(g貌u)鍩哄洜绲勫銆佸姛鑳藉熀鍥犵祫瀛稿拰姣旇純鍩哄洜绲勫
锛�2锛夌悊瑙ｇ祼(ji茅)妲�(g貌u)鍩哄洜绲勫鐨勫姛鑳藉熀鍥犵祫瀛哥殑涓昏浠诲嫏(w霉)
锛�3锛夌悊瑙ｈ綁(zhu菐n)閷勭祫瀛哥殑鐮旂┒鍏�(n猫i)瀹�
锛�4锛夌悊瑙� RNA 绲勫鐨勭爺绌跺収(n猫i)瀹�
锛�5锛夋帉鎻¤泲鐧借唱(zh矛)绲勫鐨勫畾缇�銆佺爺绌跺収(n猫i)瀹瑰拰涓昏鐨勭爺绌舵妧琛�(sh霉)
锛�6锛夌悊瑙ｈ泲鐧借唱(zh矛)鐩镐簰浣滅敤鐮旂┒鏄獚璀樿泲鐧借唱(zh矛)鍔熻兘鐨勯噸瑕佸収(n猫i)瀹�
锛�7锛夌悊瑙ｄ唬璎濈祫瀛哥殑浠诲嫏(w霉)銆佷富瑕佸垎鏋愬伐鍏峰拰鐮旂┒鍏�(n猫i)瀹�
锛�8锛夌啛鎮夌硸绲勫鍜岃剛绲勫鐨勭爺绌跺収(n猫i)瀹�
锛�9锛� 鐔熸倝绲勫鍦ㄩ啱(y墨)瀛镐笂鐨勬噳(y墨ng)鐢�
涓�銆佸弮鑰冩浉鐩�
[1] 鏌婚尗鑹富绶�. 鐢熺墿鍖栧鑸囧垎瀛愮敓鐗╁锛堢 8 鐗堬級. 浜烘皯琛�(w猫i)鐢熷嚭鐗堢ぞ, 2013
[2] 鍛傚缓鏂颁富绶�. 鍒嗗瓙鐢熺墿瀛�. 楂樼瓑鏁欒偛鍑虹増绀�, 2010
鍏嶈铂鑱叉槑锛氭湰鏂囩郴杞�(zhu菐n)杓夎嚜缍�(w菐ng)绲�(lu貌)锛屽鏈変镜鐘�锛岃珛鑱�(li谩n)绯绘垜鍊戠珛鍗冲埅闄わ紝鍙︼細鏈枃鍍呬唬琛ㄤ綔鑰呭€嬩汉瑙€榛�锛岃垏鏈恫(w菐ng)绔欑劇闂�(gu膩n)銆傚叾鍘熷壍(chu脿ng)鎬т互鍙婃枃涓櫝杩版枃瀛楀拰鍏�(n猫i)瀹规湭缍�(j墨ng)鏈珯璀夊锛屽皪鏈枃浠ュ強鍏朵腑鍏ㄩ儴鎴栬€呴儴鍒嗗収(n猫i)瀹广€佹枃瀛楃殑鐪熷鎬�銆佸畬鏁存€�銆佸強鏅傛€ф湰绔欎笉浣滀换浣曚繚璀夋垨鎵胯锛岃珛璁€鑰呭儏浣滃弮鑰冿紝骞惰珛鑷鏍稿鐩搁棞(gu膩n)鍏�(n猫i)瀹�銆�
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鎮ㄧ従(xi脿n)鍦ㄧ殑浣嶇疆锛� 
