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鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�(gu贸)400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ(xu茅)鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗�锛岄儴鍒嗗(xu茅)鏍℃洿鏂拌嚦2012骞达紝2013骞�锛涘潎鎻愪緵鏀惰不(f猫i)涓嬭級銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛�(di菐n)鎿娾€�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛�(di菐n)鎿娾€�鑰冨崥瑭﹀嵎搴�(k霉)鈥� 涓嬭級
鏄嗘槑鐞嗗伐澶у(xu茅)纰╁＋鐮旂┒鐢熷叆瀛�(xu茅)鑰冭│銆婂垎瀛愮敓鐗╁(xu茅)銆嬭€冭│澶х侗
绗竴閮ㄥ垎 鑰冭│褰㈠紡鍜岃│鍗风祼(ji茅)妲�(g貌u)
涓€銆佽│鍗锋豢鍒嗗強鑰冭│鏅�(sh铆)闁�
瑭﹀嵎婊垮垎鐐� 150 鍒�锛岃€冭│鏅�(sh铆)闁撶偤 180 鍒嗛悩锛�
浜屻€佺瓟椤屾柟寮�
绛旈鏂瑰紡鐐洪枆鍗�銆佺瓎瑭︼紟
涓�銆佽│鍗峰収(n猫i)瀹圭祼(ji茅)妲�(g貌u)
鏌撹壊楂旇垏 DNA 绱勫崰 20%
杞�(zhu菐n)閷勫強鍏惰綁(zhu菐n)閷勭敘(ch菐n)鐗╃殑鍔犲伐 绱勫崰 20%
铔嬬櫧璩�(zh矛)鐨勫悎鎴� 绱勫崰 20%
鍩哄洜琛ㄩ仈(d谩)鐨勮(di脿o)鎺� 绱勫崰 20%
鍒嗗瓙鐢熺墿瀛�(xu茅)鐮旂┒娉� 绱勫崰 10%
鍒嗗瓙鐢熺墿瀛�(xu茅)鐨勭櫦(f膩)灞曞墠娌� 绱勫崰 10%
鍥�銆佽│鍗烽鍨嬬祼(ji茅)妲�(g貌u)
瑭﹀嵎椤屽瀷绲�(ji茅)妲�(g貌u)鐐猴細
鍚嶈瑙ｉ噵 绱� 30 鍒�锛�
缈昏 绱� 20 鍒�锛�
绨�(ji菐n)绛斿拰璜栬堪 绱� 100 鍒�
绗簩閮ㄥ垎 鑰冨療鐨勭煡璀�(sh铆)鍙婅寖鍦�
涓€銆佹煋鑹查珨鑸� DNA
鑰冭│鍏�(n猫i)瀹�
鏌撹壊楂旂祼(ji茅)妲�(g貌u)锛汥NA 绲�(ji茅)妲�(g貌u)鑸囨€ц唱(zh矛)锛汥NA 鐨勫京(f霉)鍒跺拰 DNA 鐨勬悕鍌蜂慨寰�(f霉)锛汥NA 鐨勮綁(zhu菐n)搴�
鑰冭│瑕佹眰
1锛� 浜嗚В鏌撹壊楂斿拰鍩哄洜绲勭殑姒傚康
2锛� 鎺屾彙鐪熸牳鐢熺墿鏌撹壊楂旂祼(ji茅)妲�(g貌u)
3锛� 鎺屾彙 DNA 寰�(f霉)鍒剁殑鐗归粸(di菐n)
4锛� 鐞嗚В DNA 鐨勫京(f霉)鍒跺拰 DNA 鐨勬悕鍌蜂慨寰�(f霉)鍩烘湰閬�(gu貌)绋嬪拰鍒嗗瓙姗�(j墨)鍒�
5锛� 鎺屾彙鍙冭垏 DNA 寰�(f霉)鍒剁殑閰惰垏铔嬬櫧璩�(zh矛)鍥犲瓙鐨勬€ц唱(zh矛)鍜岀ó椤�
6锛� 浜嗚В杞�(zhu菐n)搴у瓙鐨勫垎椤炲拰绲�(ji茅)妲�(g貌u)鐗瑰緛锛岃綁(zhu菐n)搴т綔鐢ㄧ殑姗�(j墨)鍒�
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浜�銆佽綁(zhu菐n)閷勫強鍏惰綁(zhu菐n)閷勭敘(ch菐n)鐗╃殑鍔犲伐
鑰冭│鍏�(n猫i)瀹�
RNA 杞�(zhu菐n)閷勭殑鍩烘湰閬�(gu貌)绋�锛涘暉鍕�(d貌ng)瀛愯垏杞�(zhu菐n)閷勮捣濮嬶紝鍘熸牳鑸囩湡鏍哥敓鐗� mRNA 鐨勭壒寰佹瘮杓�锛� 绲�
姝㈠拰鎶楃祩姝�锛涘収(n猫i)鍚瓙鐨勫壀鎺�锛涘師鏍哥敓鐗╁拰鐪熸牳鐢熺墿鍚勭ó椤炲瀷 RNA 鐨勫姞宸ラ亷(gu貌)绋�锛�
鑰冭│瑕佹眰
1. 鍏ㄩ潰浜嗚В RNA 杞�(zhu菐n)閷勮垏寰�(f霉)鍒剁殑姗�(j墨)鍒�
2. 鎺屾彙杞�(zhu菐n)閷勭殑涓€鑸(gu墨)寰�
3. 鎺屾彙鍟熷嫊(d貌ng)瀛愮殑绲�(ji茅)妲�(g貌u)鐗归粸(di菐n)鍜屽姛鑳�
4. 鐞嗚В RNA 杞�(zhu菐n)閷勫悗鍔犲伐閬�(gu貌)绋嬪強鍏舵剰缇�
5. 鎺屾彙閫嗚綁(zhu菐n)閷勭殑閬�(gu貌)绋�
锛�
涓夈€佽泲鐧借唱(zh矛)鐨勫悎鎴�
鑰冭│鍏�(n猫i)瀹�
tRNA 鐨勭祼(ji茅)妲�(g貌u)鐗归粸(di菐n)锛涗笁椤� RNA 鍦ㄨ泲鐧借唱(zh矛)鐢熺墿鍚堟垚涓殑浣滅敤锛涢伜鍌冲瘑纰硷細瀵嗙⒓瀛愮殑绨�(ji菐n)骞�
鎬�锛屾悥鎿烘€э紱绶氱矑楂斿瘑纰煎瓙鐨勭壒娈婃€�锛涜泲鐧借唱(zh矛)鍚堟垚鐨勯亷(gu貌)绋�锛涘弮鑸囪泲鐧借唱(zh矛)鍚堟垚鐨勪富瑕佸垎瀛愮殑绋�
椤炲拰鍔熻兘锛涜泲鐧借唱(zh矛)纾烽吀鍖栬垏绯栧熀鍖栫殑鐢熺墿瀛�(xu茅)鎰忕京锛涜泲鐧借唱(zh矛)宸ョ▼鐨勯€�(j矛n)灞曠瓑銆�
鑰冭│瑕佹眰
1. 鍏ㄩ潰浜嗚В铔嬬櫧璩�(zh矛)鍚堟垚鐨勯亷(gu貌)绋嬪強涓昏鍒嗗瓙鐨勭ó椤炲拰鍔熻兘
2. 鐔熺反鎺屾彙铔嬬櫧璩�(zh矛)鍚堟垚涓ā鏉垮拰閬哄偝瀵嗙⒓鐨勭壒榛�(di菐n)
3. 鎺屾彙铔嬬櫧璩�(zh矛)鍚堟垚鐨勯亷(gu貌)绋嬪拰鑲介張鐨勫悗鍔犲伐閬�(gu貌)绋�
4. 鐞嗚В鐪熸牳鐢熺墿鑸囧師鏍哥敓鐗╄泲鐧借唱(zh矛)鍚堟垚鐨勫崁(q奴)鍒�
鍥�銆� 鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺�
鑰冭│鍏�(n猫i)瀹�
鍘熸牳鐢熺墿鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺� 鎿嶇副瀛�銆佹瑾�(di脿o)鎺ц垏璨�(f霉)瑾�(di脿o)鎺х殑姒傚康;. 骞剧ó鎿嶇副瀛愮殑绲勬垚鍙婂叾瑾�(di脿o)
鎺�锛屽涔崇硸鎿嶇副瀛�锛岃壊姘ㄩ吀鎿嶇副瀛�锛屽崐涔崇硸鎿嶇副瀛愶紝闃挎媺浼硸鎿嶇副瀛�; 琛版笡瀛愮殑妲�(g貌u)鎴愬強鍏惰(di脿o)
鎺ф(j墨)鐞�; 鍙嶇京 RNA 鍙婂叾瑾�(di脿o)鎺т綔鐢�
鐪熸牳鐢熺墿鍩哄洜琛ㄩ仈(d谩)鐨勮(di脿o)鎺� 鐪熸牳绱�(x矛)鑳炵殑鍩哄洜绲�(ji茅)妲�(g貌u); 閶呮寚绲�(ji茅)妲�(g貌u)锛涜灪鏃�-鐠�(hu谩n)-铻烘棆绲�(ji茅)妲�(g貌u)锛涗寒姘�
閰告媺閺堢祼(ji茅)妲�(g貌u)锛涙寔瀹跺熀鍥犲拰濂緢鍩哄洜; 鐪熸牳鐢熺墿杞�(zhu菐n)閷勫悗鍜岀炕璀悗鍔犲伐锛涢爢寮忎綔鐢ㄥ厓浠惰垏鍩哄洜瑾�(di脿o)
鎺э紱鍙嶅紡浣滅敤鍥犲瓙灏�(du矛)杞�(zhu菐n)閷勭殑瑾�(di脿o)鎺�锛涚湡鏍哥敓鐗╂柗瑁傚熀鍥犵殑姒傚康鍜岀祼(ji茅)妲�(g貌u)鐗归粸(di菐n)銆侀伕鎿囨€у壀鍒囩瓑姒傚康
鍙婃剰缇╃瓑?锛涱媰铏_(d谩)鑸� DNA 鐢插熀鍖栫瓑
鑰冭│瑕佹眰
1. 鐞嗚В杞�(zhu菐n)閷勬按骞充笂鐨勫熀鍥犺〃閬�(d谩)瑾�(di脿o)鎺у拰缈昏姘村钩涓婄殑鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺�
2. 鎺屾彙鍟熷嫊(d貌ng)瀛愯垏杞�(zhu菐n)閷勮捣濮�
3. 鎺屾彙涔崇硸鎿嶇副瀛愭ā鍨�
4. 鎺屾彙鐪熸牳鐢熺墿 DNA 姘村钩鐨勮(di脿o)鎺�
5. 鎺屾彙闋嗗紡浣滅敤鍏冧欢鑸囧熀鍥犺(di脿o)鎺�
6. 鎺屾彙鍙嶅紡浣滅敤鍥犲瓙灏�(du矛)杞�(zhu菐n)閷勭殑瑾�(di脿o)鎺�.
浜斻€佸垎瀛愮敓鐗╁(xu茅)鐮旂┒娉�
鑰冭│鍏�(n猫i)瀹�
DNA銆丷NA銆佽泲鐧借唱(zh矛)鎿嶄綔鎶€琛�(sh霉)鍙婂熀鍥犲姛鑳界爺绌舵妧琛�(sh霉)
鑰冭│瑕佹眰
1锛� 浜嗚В鏍搁吀鐨勬彁鍙�銆佺磾鍖栥€侀浕娉冲拰娓�(c猫)搴忕殑鍘熺悊鍜屼竴鑸椹�
2锛� 浜嗚В DNA 浜炲厠闅嗙殑鍏烽珨姝ラ
3锛� 鎺屾彙 PCR銆丷T-PCR 鎶€琛�(sh霉)鐨勫熀鏈師鐞嗗拰鎿嶄綔鐨勪竴鑸椹熷強鍏舵噳(y墨ng)鐢�
4锛� 浜嗚В DNA 鍒嗗瓙妯�(bi膩o)瑷�
5锛� 鎺屾彙 Southern blotting銆丯orthern blotting 鍜� Western blotting 鐨勫師鐞�锛屾噳(y墨ng)鐢ㄨ寖鍦嶅強鍏舵搷
浣滄椹�
6锛� 浜嗚В鍩哄洜绲勬枃搴�(k霉)鍜� cDNA 鏂囧韩(k霉)鐨勬(g貌u)寤�
7锛� 浜嗚В铔嬬櫧璩�(zh矛)绲勫(xu茅)鐨勬蹇靛強鍏剁爺绌舵妧琛�(sh霉)
8锛� 鎺屾彙 RNA 骞叉摼鐨勫師鐞嗗強鍏舵噳(y墨ng)鐢�
9锛� 鎺屾彙鍩哄洜宸ョ▼鐨勫師鐞嗗強鍏朵竴鑸椹�
10锛� 閬�(y霉n)鐢ㄥ悇绋垎瀛愮敓鐗╁(xu茅)鎶€琛�(sh霉)鐨勫(sh铆)椹�(y脿n)瑷�(sh猫)瑷�(j矛)鍜岀爺绌剁祼(ji茅)鏋滃垎鏋�
鍏�銆佸垎瀛愮敓鐗╁(xu茅)鐧�(f膩)灞曞墠娌�
鑰冭│鍏�(n猫i)瀹�
鍒嗗瓙鐢熺墿瀛�(xu茅)鐞嗚珫鍜屾妧琛�(sh霉)鍦ㄧ柧鐥呰垏浜洪鍋ュ悍鐮旂┒闋�(l菒ng)鍩熺殑鐧�(f膩)灞曞墠娌�
鑰冭│瑕佹眰
浜嗚В鍒嗗瓙鐢熺墿瀛�(xu茅)鍦ㄧ柧鐥呰垏浜洪鍋ュ悍鐮旂┒闋�(l菒ng)鍩熺殑鐧�(f膩)灞曡定鍕�(sh矛)
鍏嶈铂(z茅)鑱叉槑锛氭湰鏂囩郴杞�(zhu菐n)杓夎嚜缍�(w菐ng)绲�(lu貌)锛屽鏈変镜鐘�锛岃珛(q菒ng)鑱�(li谩n)绯绘垜鍊戠珛鍗冲埅闄わ紝鍙︼細鏈枃鍍呬唬琛ㄤ綔鑰呭€�(g猫)浜鸿榛�(di菐n)锛岃垏鏈恫(w菐ng)绔欑劇(w煤)闂�(gu膩n)銆傚叾鍘熷壍(chu脿ng)鎬т互鍙婃枃涓櫝杩版枃瀛楀拰鍏�(n猫i)瀹规湭缍�(j墨ng)鏈珯璀夊(sh铆)锛屽皪(du矛)鏈枃浠ュ強鍏朵腑鍏ㄩ儴鎴栬€呴儴鍒嗗収(n猫i)瀹�銆佹枃瀛楃殑鐪熷(sh铆)鎬�銆佸畬鏁存€�銆佸強鏅�(sh铆)鎬ф湰绔欎笉浣滀换浣曚繚璀夋垨鎵胯锛岃珛(q菒ng)璁€鑰呭儏浣滃弮鑰�锛屽苟璜�(q菒ng)鑷鏍稿(sh铆)鐩搁棞(gu膩n)鍏�(n猫i)瀹�銆�
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鏂囩珷鎼滅储
鎮ㄧ従(xi脿n)鍦ㄧ殑浣嶇疆锛� 
