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鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ(xu茅)鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗�锛岄儴鍒嗗(xu茅)鏍℃洿鏂拌嚦2012骞达紝2013骞�锛涘潎鎻愪緵鏀惰不涓嬭級銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛炴搳鈥�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛炴搳鈥�鑰冨崥瑭﹀嵎搴�鈥� 涓嬭級
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涓€銆佽€冭│褰㈠紡鑸囪│鍗风祼(ji茅)妲�(g貌u)
1锛� 瑭﹀嵎鎴愮妇鍙婅€冭│鏅傞枔锛�
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2锛夌瓟椤屾柟寮忥細
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3锛夎│鍗峰収(n猫i)瀹圭祼(ji茅)妲�(g貌u)
锛堜竴锛夐潨鎱�(t脿i) 閮ㄥ垎 绱� 30%
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4锛夐鍨嬬祼(ji茅)妲�(g貌u)
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锛堜竴锛夎€冭│鍏�(n猫i)瀹�
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姘ㄥ熀閰哥殑鍒嗚В浠ｈ瑵锛岄伜鍌冲瘑纰硷紝铔嬬櫧璩�(zh矛)鐨勫悎鎴�銆佺炕璀悗淇＞鍙婅綁(zhu菐n)閬嬶紝铔嬬櫧璩�(zh矛)鐨勫垎闆㈢磾鍖栬垏
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2銆佹牳閰哥殑绲�(ji茅)妲�(g貌u)銆佹€ц唱(zh矛)鍙婄祼(ji茅)妲�(g貌u)鑸囨€ц唱(zh矛)鐨勯棞(gu膩n)绯�锛孌NA 鐨勫悎鎴�锛孯NA 鐨勫悎鎴愯垏鍔犲伐锛孌NA 鐨勬悕
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4銆侀叾鐨勭祼(ji茅)妲�(g貌u)鍙婄祼(ji茅)妲�(g貌u)鑸囧姛鑳界殑闂�(gu膩n)绯�锛岄叾浣滅敤鐨勫皥涓€鎬�锛岄叾鐨勪綔鐢ㄦ鍒讹紝閰朵績鍙嶆噳(y墨ng)鍕曞姏
瀛�(xu茅)锛岄叾娲绘€х殑瑾�(di脿o)绡€(ji茅)锛岄叾鑸囩董鐢熺礌鐨勯棞(gu膩n)绯�銆�
5銆佺敓鐗╂哀鍖栫殑姒傚康鑸囩壒榛�锛岄珮鑳界７閰稿寲鍚堢墿锛岄浕瀛愬偝閬炲拰姘у寲鍛煎惛閺�锛屾哀鍖栫７閰稿寲浣�
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7銆佽剛椤炵墿璩�(zh矛)鐨勭祼(ji茅)妲�(g貌u)銆佹€ц唱(zh矛)鍙婄祼(ji茅)妲�(g貌u)鑸囧姛鑳界殑闂�(gu膩n)绯�锛岃剛鑲吀鐨勬哀鍖�锛岄叜楂旂殑鐢熸垚鑸囧埄鐢�锛�
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锛堜簩锛夎€冭│瑕佹眰
1銆� 姘ㄥ熀閰歌垏铔嬬櫧璩�(zh矛)
锛�1锛夋帉鎻� 20 绋(bi膩o)婧�(zh菙n)姘ㄥ熀閰哥殑鍚嶇ū銆佺铏熴€佺祼(ji茅)妲�(g貌u)鍙婁富瑕佹€ц唱(zh矛)銆�
锛�2锛夋帉鎻¤泲鐧借唱(zh矛)涓€銆佷簩銆佷笁銆佸洓绱氱祼(ji茅)妲�(g貌u)鐨勭浉闂�(gu膩n)姒傚康銆佺壒榛�銆�
锛�3锛変簡瑙ｄ簽绱�(x矛)鑳炲垎绱氬垎闆㈢殑鍩烘湰鏂规硶鍜屽師鐞嗭紱鎺屾彙铔嬬櫧璩�(zh矛)鍒嗛洟绱斿寲鐨勫熀鏈椹熷拰娉ㄦ剰
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锛�5锛変簡瑙ｈ泲鐧借唱(zh矛)搴忓垪娓畾鐨勪竴鑸柟娉�銆�
锛�6锛夌啛绶存帉鎻℃皑鍩洪吀闄嶈В鐨勪富瑕佹柟寮忓拰鐗归粸銆�
锛�7锛変簡瑙ｆ皑鍩洪吀鐢熺墿鍚堟垚鐨勭壒榛�锛屾皑鍩洪吀浠ｈ瑵鐣板父灏�(d菐o)鑷寸殑涓昏鐤剧梾銆�
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鏈亷绋嬪拰鐗归粸銆�
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2銆佹牳閰�
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锛�5锛夋帉鎻� RNA 杞�(zhu菐n)閷勫悗鍔犲伐鐨勪富瑕佸収(n猫i)瀹�銆佹柟寮忓拰鐗归粸銆�
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锛�7锛夋帉鎻￠檺鍒舵€у収(n猫i)鍒囬叾锛岄檺鍒舵€х墖娈甸暦搴﹀鎱�(t脿i)鎬�銆佹牳閰搁洔浜�锛孲outhern 鍗拌贰锛�
Northern 鍗拌贰锛屽師浣嶉洔浜�锛孌NA 鍏嬮殕锛孌NA 鏂囧韩锛孭CR 绛夊熀鏈蹇�銆�
3銆佺敓鐗╄啘
锛�1锛変簡瑙ｇ敓鐗╄啘鐨勭祫鎴�銆佺祼(ji茅)妲�(g貌u)鍜岀壒榛�銆�
锛�2锛夋帉鎻′富鍕曡綁(zhu菐n)閬�锛岃鍕曡綁(zhu菐n)閫侊紝鑳炲悶锛岃優鍚�锛岃優椋�锛屽悶鍣�锛屽彈楂斾粙灏�(d菐o)鐨勮優鍚�锛屾暣鍚堣啘
铔嬬櫧锛屽鍛ㄨ啘铔嬬櫧锛岀窗(x矛)鑳炰俊铏�锛岀浜屼俊浣跨瓑鑸囩墿璩�(zh矛)璺ㄨ啘杞�(zhu菐n)閬嬪拰绱�(x矛)鑳炰俊铏熷偝灏�(d菐o)鏈夐棞(gu膩n)鐨勫熀鏈
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4銆侀叾
锛�1锛夋帉鎻￠叾锛岃紨鍥犲瓙锛屾椿鎬т綅榛�锛屽簳鐗╃壒鐣版€�锛屽悓宸ラ叾锛屽垾妲�(g貌u)閰讹紝绫虫皬鏂圭▼锛屾湭姘忓父
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锛�2锛夌啛绶存帉鎻￠叾淇冨弽鎳�(y墨ng)閫熷害鍙婂奖闊块叾淇冨弽鎳�(y墨ng)閫熷害鐨勪富瑕佸洜绱�锛屽寘鎷叾鐨勬姂鍒朵綔鐢ㄧ殑姒�
蹇�銆佸彲閫嗗拰涓嶅彲閫嗙殑鎶戝埗浣滅敤锛屼互鍙婂彲閫嗘姂鍒朵綔鐢ㄧ殑鍏╃ó涓昏椤炲瀷鍙婂叾鐗归粸銆�
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5銆佺敓鐗╂哀鍖�
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锛�2锛夋帉鎻″懠鍚搁張锛屾哀鍖栫７閰稿寲锛岃В鍋惰伅(li谩n)鍔�锛屽寲瀛�(xu茅)婊查€忓亣瑾殑姒傚康鍙婁富瑕佺浉闂�(gu膩n)鐭ヨ瓨榛炪€�
6銆佺硸
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锛�2锛夌啛绶存帉鎻＄硸閰佃В銆佹妾吀寰挵(hu谩n)鍜岀硸鐣扮敓鐨勬湁闂�(gu膩n)鐭ヨ瓨銆�
锛�3锛夋帉鎻＄７閰告垔绯栭€斿緫锛岀硸鍘熶唬璎濈殑鍩烘湰閬庣▼鑸囩壒榛炪€�
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7銆佽剛椤�
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锛�2锛夋帉鎻＄窗(x矛)鑳炰唬璎濈殑鍩烘湰鏂瑰紡鍜岀壒榛炪€�
涓�銆佸弮鑰冩浉鐩�
1. B.D.Hames 锛� N.M. Hooper. 銆� Biochemistry 銆� (Third Edition) 锛� Science
Press.2009.
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