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鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�(gu贸)400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ(xu茅)鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗�锛岄儴鍒嗗(xu茅)鏍℃洿鏂拌嚦2012骞�锛�2013骞�锛涘潎鎻愪緵鏀惰不(f猫i)涓嬭級銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛�(di菐n)鎿娾€�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛�(di菐n)鎿娾€�鑰冨崥瑭﹀嵎搴�(k霉)鈥� 涓嬭級
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鑰冭│绉戠洰浠ｇ⒓锛� 鑰冭│绉戠洰鍚嶇ū锛氬垎瀛愮敓鐗╁(xu茅)
涓€銆佽€冭│褰㈠紡鑸囪│鍗风祼(ji茅)妲�(g貌u)
1) 瑭﹀嵎鎴愮妇(j墨)鍙婅€冭│鏅�(sh铆)闁�
鏈│鍗锋豢鍒嗙偤 100 鍒�锛岃€冭│鏅�(sh铆)闁撶偤 180 鍒嗛悩銆�
2) 绛旈鏂瑰紡
绛旈鏂瑰紡鐐洪枆鍗�銆佺瓎瑭�銆�
3) 瑭﹀嵎鍏�(n猫i)瀹圭祼(ji茅)妲�(g貌u)
鍚勯儴鍒嗗収(n猫i)瀹规墍鍗犲垎鍊肩偤锛�
鍩哄洜绲�(ji茅)妲�(g貌u)鑸� DNA 閲嶇祫鎶€琛�(sh霉) 绱� 40 鍒�
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鍩哄洜绲勮垏鐢熺墿淇℃伅瀛�(xu茅)鐮旂┒ 绱� 20 鍒�
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鏄潪椤岋細 10 灏忛锛屾瘡灏忛 1 鍒�锛屽叡 10 鍒�
绨�(ji菐n)绛旈锛� 4 灏忛锛屾瘡灏忛 10 鍒�锛屽叡 40 鍒�
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浜�銆佽€冭│鍏�(n猫i)瀹硅垏鑰冭│瑕佹眰
绗竴绔� 绶掕珫
鑰冭│瑕佹眰
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鍥犵祫瀛�(xu茅)銆佸姛鑳藉熀鍥犵祫鑸囩敓鐗╀俊鎭(xu茅)绛夌浉闂�(gu膩n)姒傚康銆�
鑰冭│鍏�(n猫i)瀹�
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鍩哄洜姒傚康鐨勭櫦(f膩)灞曞強鍏跺垎瀛愮敓鐗╁(xu茅)瀹氱京
涓績娉曞墖鐨勪富瑕佸収(n猫i)瀹瑰強鍏剁櫦(f膩)灞曢亷(gu貌)绋�
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锛堜笁锛夊垎瀛愮敓鐗╁(xu茅)鐧�(f膩)灞曞彶
鍒嗗瓙鐢熺墿瀛�(xu茅)鐧�(f膩)灞曟绋嬩腑鐨勯噷绋嬬銆傛垜鍦�(gu贸)绉戝(xu茅)瀹跺湪鍒嗗瓙鐢熺墿瀛�(xu茅)鐧�(f膩)灞曟绋嬩腑
鐨勮并鐛�(xi脿n)
鍒嗗瓙鐢熺墿瀛�(xu茅)灏�(du矛)鐢熺墿瀛�(xu茅)鍏跺畠瀛�(xu茅)绉戠殑鎺ㄥ嫊(d貌ng)锛屽垎瀛愮敓鐗╁(xu茅)鐨勭櫦(f膩)灞曡定鍕�(sh矛)
绗簩绔� 鏌撹壊楂旇垏 DNA 寰�(f霉)鍒�
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寰�(f霉)鍒舵柟寮�锛屽師鏍哥敓鐗╁拰鐪熸牳鐢熺墿 DNA 鐨勫京(f霉)鍒剁壒榛�(di菐n)锛孌NA 鐨勪慨寰�(f霉)姗�(j墨)鍒�銆�
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锛堜竴锛夋煋鑹查珨鐨勭祫鎴愬拰绲�(ji茅)妲�(g貌u)鐗归粸(di菐n)锛孌NA 寰�(f霉)鍒剁殑姗�(j墨)鍒�锛孌NA 淇京(f霉)鐨勬柟寮�锛岄伜鍌�
閲嶇祫鐨勭櫦(f膩)鐢熸(j墨)鍒讹紝DNA 杞�(zhu菐n)搴х殑閬哄偝鏁堟噳(y墨ng)
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锛堜笁锛塂NA 寰�(f霉)鍒剁殑瑾�(di脿o)鎺�锛岀湡鏍哥敓鐗╁京(f霉)鍒堕叾绯伙紝杞�(zhu菐n)搴у瓙鐨勫垎椤�
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榛�(di菐n)浠ュ強瑾�(di脿o)鎺х殑涓嶅悓灞ゆ锛岀湡鏍哥敓鐗� DNA 鑱氬悎閰剁殑鍒嗛鐨勭壒鎬�锛屽師鏍哥敓鐗╁拰鐪熸牳鐢�
鐗╀腑杞�(zhu菐n)搴у瓙鐨勯鍨�銆�
绗笁绔� RNA 鐨勮綁(zhu菐n)閷�
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mRNA 绲�(ji茅)妲�(g貌u)鐗归粸(di菐n)
杞�(zhu菐n)閷勭殑涓€鑸(gu墨)寰嬪拰閬�(gu貌)绋嬶紝鍘熸牳鐢熺墿 RNA 鑱氬悎閰剁祼(ji茅)妲�(g貌u)鍙婂悇鍊�(g猫)浜炲熀鐨勪綔鐢�锛岀湡鏍�
鐢熺墿 RNA 鑱氬悎閰剁殑鍒嗛銆佺壒鎬у強鍏惰綁(zhu菐n)閷勭敘(ch菐n)鐗╃殑宸暟锛屽暉鍕�(d貌ng)瀛愬拰澧炲挤(qi谩ng)瀛愮殑绲�(ji茅)妲�(g貌u)鍜屼綔
鐢ㄧ壒榛�(di菐n)锛岃綁(zhu菐n)閷勭祩姝㈠拰鎶楃祩姝�
RNA 杞�(zhu菐n)閷勮垏 DNA 寰�(f霉)鍒堕亷(gu貌)绋嬬殑宸暟锛孯NA 杞�(zhu菐n)閷勫悗鍔犲伐閬�(gu貌)绋嬪強鍏舵剰缇╋紝鍘熸牳鐢熺墿
鑸囩湡鏍哥敓鐗� mRNA 鐨勭壒寰佹瘮杓�
RNA 鑱氬悎閰惰垏鍟熷嫊(d貌ng)瀛愮殑绲�(ji茅)鍚�锛孯NA 鐢熺墿瀛�(xu茅)鍔熻兘鐨勫妯ｆ€�
RNA 琛屼娇鐨勫绋敓鐗╁(xu茅)鍔熻兘
鍟熷嫊(d貌ng)瀛愮祼(ji茅)妲�(g貌u)鏀硅畩灏�(du矛) RNA 杞�(zhu菐n)閷勬晥鐜囩殑褰遍熆
锛堜簩锛塕NA 鐨勬嫾鎺�
鍓嶉珨 mRNA 涓収(n猫i)鍚瓙鑸囧椤瓙浜ょ晫搴忓垪鐨勭祼(ji茅)妲�(g貌u)鐗瑰緛锛屽収(n猫i)鍚瓙鐨勫壀鍒囨柟寮�
绗洓绔� 铔嬬櫧璩�(zh矛)鐨勭炕璀�
鑰冭│瑕佹眰
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鎴愬拰鎬ц唱(zh矛)銆乼RNA 鐨勪簩绱�(j铆)绲�(ji茅)妲�(g貌u)鑸囧弽瀵嗙⒓瀛愩€佹牳绯栭珨鐨勭祫鎴愯垏鍔熻兘銆傞噸榛�(di菐n)鐞嗚В骞舵帉
鎻¤泲鐧借唱(zh矛)鐢熺墿鍚堟垚閬�(gu貌)绋�锛屼簡瑙ｄ俊铏�(h脿o)鑲藉強鍏跺湪缈昏鈥斺€旈亱(y霉n)杞�(zhu菐n)鍚屾姗�(j墨)鍒朵腑鐨勪綔鐢�銆�
鑰冭│鍏�(n猫i)瀹�
锛堜竴锛夎泲鐧借唱(zh矛)缈昏閬�(gu貌)绋�锛屽弮鑸囪泲鐧借唱(zh矛)鍚堟垚鐨勫厓浠�
涓夎伅(li谩n)楂旈伜鍌冲瘑纰肩殑姒傚康锛岄伜鍌冲瘑纰肩殑鐗规€�锛宼RNA 鐨勭祼(ji茅)妲�(g貌u)鍙婂叾鍦ㄨ泲鐧借唱(zh矛)鐢�
鐗╁悎鎴愪腑鐨勪綔鐢�锛屾牳绯栭珨鐨勭祼(ji茅)妲�(g貌u)鍙婂叾鍦ㄨ泲鐧借唱(zh矛)鐢熺墿鍚堟垚涓殑浣滅敤
铔嬬櫧璩�(zh矛)缈昏鐨勬椹燂紝鐪熸牳鑸囧師鏍哥敓鐗╃炕璀捣濮嬬殑鍗€(q奴)鍒�
锛堜簩锛夎泲鐧借唱(zh矛)杞�(zhu菐n)閬�(y霉n)姗�(j墨)鍒�
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姗�(j墨)鍒�锛屾牳瀹氫綅铔嬬櫧鐨勮綁(zhu菐n)閬�(y霉n)姗�(j墨)鍒讹紝铔嬬櫧璩�(zh矛)鐨勯檷瑙�
锛堜笁锛夎泲鐧借唱(zh矛)鍓嶉珨鐨勫姞宸�锛岃泲鐧借唱(zh矛)鍚堟垚鎶戝埗鍔�
绗簲绔� 鍒嗗瓙鐢熺墿瀛�(xu茅)鐮旂┒鏂规硶
鑰冭│瑕佹眰
浜嗚В鍒嗗瓙鐢熺墿瀛�(xu茅)鐩墠甯哥敤鐨勫熀鏈爺绌舵柟娉曘€傜悊瑙ｅ苟鎺屾彙鏍搁吀鐨勫嚌鑶犻浕娉�锛屾牳
閰哥殑鍒嗗瓙闆滀氦銆丳CR 鎶€琛�(sh霉)銆丏NA 閲嶇祫鎶€琛�(sh霉)銆丏NA 鎸囩磱鎶€琛�(sh霉)鎿嶄綔閬�(gu貌)绋�銆�
鑰冭│鍏�(n猫i)瀹�
锛堜竴锛塂NA 鎿嶄綔鎶€琛�(sh霉)锛岄噸绲� DNA 涓父鐢ㄧ殑宸ュ叿閰�
鍒嗗瓙鍏嬮殕鐨勬蹇靛拰閬�(gu貌)绋�锛屽嚌鑶犻浕娉崇殑宸ヤ綔鍘熺悊鍜屾噳(y墨ng)鐢�锛屽垎瀛愰洔浜ょ殑瀵�(sh铆)椹�(y脿n)娴�
绋嬪拰鍒嗛锛岄檺鍒舵€у収(n猫i)鍒囬叾鐨勯鍒ュ拰鎳�(y墨ng)鐢�锛孌NA 閫ｆ帴閰跺拰閫嗚綁(zhu菐n)閷勯叾姒傚康鍜屾噳(y墨ng)鐢�锛�
DNase I 瓒宠贰瀵�(sh铆)椹�(y脿n)锛屽嚌鑶犻樆婊(sh铆)椹�(y脿n)
闆欒劔姘ф硶娓�(c猫)搴忕殑宸ヤ綔娴佺▼鍜屽師鐞�锛岃仛鍚堥叾閺�?zh菙n)妞掔；?y墨ng)锛圥CR 鎶€琛�(sh霉)锛夌殑宸ヤ綔娴�
绋嬨€佸師鐞嗗拰鎳�(y墨ng)鐢�
DNA 閲嶇祫鎶€琛�(sh霉)灏�(du矛)浜庡熀绀�(ch菙)鐮旂┒鍜岀敓鐢�(ch菐n)瀵�(sh铆)韪愮殑鎰忕京
锛堜簩锛夊熀鍥犵殑鍒嗛洟鍜岄憭瀹�
鍩哄洜鍏嬮殕鐨勪富瑕佽級楂旂郴绲�(t菕ng)锛岃〃閬�(d谩)杓夐珨鎳�(y墨ng)鍏峰倷鐨勫熀鏈壒榛�(di菐n)锛屽熀鍥犵祫鏂囧韩(k霉)鐨勬
蹇靛拰妲�(g貌u)寤烘柟娉�锛宑DNA 鏂囧韩(k霉)鐨勬蹇靛拰妲�(g貌u)寤烘柟娉�锛岀洰鐨勫熀鍥犵殑绡╅伕鏂规硶
鍩哄洜绲勬枃搴�(k霉)鍜� cDNA 鏂囧韩(k霉)鐨勫樊鐣�
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锛堜笁锛夊垎瀛愬厠闅嗘妧琛�(sh霉)
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绗崄绔� 鍩哄洜绲勮垏姣旇純鍩哄洜绲勫(xu茅)
鑰冭│瑕佹眰
鍦ㄤ簡瑙ｅ熀鍥犵祫瑷�(j矛)鍔冪殑鐩(bi膩o)鍜岀瀛�(xu茅)鎰忕京鐨勫熀绀�(ch菙)涓�锛屼簡瑙ｅ熀鍥犵祫 DNA 澶х墖鏂锋枃搴�(k霉)
鐨勬(g貌u)寤�锛屾帉鎻″叏鍩哄洜绲勯偿(ni菐o)妲嶆硶娓�(c猫)搴忕殑鍩烘湰鍘熺悊锛屼簡瑙ｅ悗鍩哄洜绲勭爺绌剁殑鏈夐棞(gu膩n)鍟�(w猫n)椤�锛�
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瀹瑰拰鎶€琛�(sh霉)璺窔
涓�銆佸弮鑰冩浉(sh奴)鐩�
[1] 鏈辩帀璩紝绛夌法. 鐝�(xi脿n)浠ｅ垎瀛愮敓鐗╁(xu茅)锛堢涓夌増锛�. 楂樼瓑鏁欒偛鍑虹増绀�, 2007
[2] 閯敤鐠� 绶�. 鍩虹(ch菙)鍒嗗瓙鐢熺墿瀛�(xu茅)锛堢浜岀増锛�. 楂樼瓑鏁欒偛鍑虹増绀�, 2012
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鎮ㄧ従(xi脿n)鍦ㄧ殑浣嶇疆锛� 
