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榛�(di菐n)銆傚畠鏄棦瀹规槗寰楀垎锛屽張瀹规槗涓熷垎鐨勯鐩�銆傞€欓椤屼笉闇€瑕佸(xu茅)鐢熷湪寰�(f霉)缈�(x铆)鏅�(sh铆)姝昏纭儗锛�
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鍔涘拰閭忚集鎬濈董鑳藉姏绛�銆�
绛旈瑕佹眰
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锛�2锛夊皪(du矛)浜庡～绌洪锛岃姹傚～绌鸿婧�(zh菙n)纰猴紝鐒￠渶瑙ｉ噵銆�
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姣旇純瑭崇窗(x矛)鍜屽厖鍒嗙殑瑙ｉ噵鍜岃鏄�銆傚鏋滃彧绛旇榛�(di菐n)涓嶄綔瑙ｉ噵鍜岃鏄庯紝灏辫鎵ｅ垎銆�
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1. 閰剁殑鍩烘湰姒傚康
璀�(sh铆)瑷橈細 閰舵椿鍔涚殑娓畾鏂规硶銆�
鐞嗚В锛� 閰跺伐绋嬬櫦(f膩)灞曟娉併€�
2. 閰剁殑鐢熺敘(ch菐n)鏂规硶
璀�(sh铆)瑷橈細 閰剁殑鐢熺敘(ch菐n)鏂规硶銆�
鎳�(y墨ng)鐢細 閰剁殑鎳�(y墨ng)鐢ㄥ墠鏅�銆�
绗簩绔� 閰剁殑鐧�(f膩)閰电敓鐢�(ch菐n)
1. 閰剁殑鐧�(f膩)閰电敓鐢�(ch菐n)涓昏鏂规硶
璀�(sh铆)瑷橈細 鍥洪珨鐧�(f膩)閰靛煿椁�(y菐ng)銆佹恫楂旀繁灞ょ櫦(f膩)閰典互鍙婂浐瀹氬寲绱�(x矛)鑳炵櫦(f膩)閰�銆�
鐞嗚В锛� 骞剧ó鐧�(f膩)閰电殑鍎�(y艒u)缂洪粸(di菐n)銆�
2. 閰剁敓鐗╁悎鎴愮殑鍩烘湰鐞嗚珫
璀�(sh铆)瑷橈細 杞�(zhu菐n)閷�銆佺炕璀€�
鐞嗚В锛� 閰剁敓鐗╁悎鎴愮殑瑾�(di脿o)绡€(ji茅)銆�
3. 閰剁殑鐧�(f膩)閰靛伐钘濊垏鐧�(f膩)閰靛嫊(d貌ng)鍔涘(xu茅)
鐞嗚В锛氶叾鐢熺墿鍚堟垚鐨勬ā寮�
绗笁绔� 閰剁殑鍒嗛洟绱斿寲
1. 绱�(x矛)鑳炵牬纰�
璀�(sh铆)瑷橈細 绱�(x矛)鑳炵牬纰庣殑鏂规硶銆�
2. 閰剁殑鎻愬彇
璀�(sh铆)瑷橈細 閰剁殑鎻愬彇鏂规硶銆�
3. 闆㈠績鍒嗛洟
璀�(sh铆)瑷橈細 闆㈠績姗�(j墨)鐨勪娇鐢ㄨ垏鐢ㄩ€斻€�
鐞嗚В锛� 闆㈠績鏂规硶鐨勯伕鎿�銆�
4. 閬庢烤鑸囪啘鍒嗛洟
璀�(sh铆)瑷橈細 閬庢烤鑸囪啘鍒嗛洟銆�
5. 娌夋穩鍒嗛洟
璀�(sh铆)瑷橈細 楣芥瀽娌夋穩娉曘€佹湁姗�(j墨)婧跺姂娌夋穩娉�銆佺瓑闆婚粸(di菐n)娌夋穩娉曚互鍙婂京(f霉)鍚堟矇娣€娉�銆�
6. 灞ゆ瀽鍒嗛洟
璀�(sh铆)瑷橈細 鍚搁檮灞ゆ瀽銆侀洟瀛愪氦鎻涘堡鏋�銆佸嚌鑶犲堡鏋愩€佽Κ鍜屽堡鏋�銆�
鐞嗚В锛� 鍚搁檮銆侀洟瀛愪氦鎻�銆佸嚌鑶�銆佽Κ鍜屽堡鏋愮殑鍘熺悊銆�
鎳�(y墨ng)鐢細 瀛�(xu茅)鏈�(hu矛)骞剧ó灞ゆ瀽鏂规硶鐨勬噳(y墨ng)鐢�
7. 闆绘吵鍒嗛洟
璀�(sh铆)瑷橈細 鍑濊啝闆绘吵鍜岀瓑闆昏仛鐒﹂浕娉�銆�
8. 閰剁殑绲�(ji茅)鏅�
鐞嗚В锛� 楣芥瀽绲�(ji茅)鏅舵硶銆佹湁姗�(j墨)婧跺姂绲�(ji茅)鏅舵硶銆佺瓑闆婚粸(di菐n)绲�(ji茅)鏅舵硶銆�
9. 婵冪府鑸囧共鐕�
璀�(sh铆)瑷橈細 婵冪府鑸囧共鐕�銆�
绗洓绔� 閰剁殑鍒嗗瓙淇＞
閰剁殑鍖栧(xu茅)淇＞
鐞嗚В锛氶叾鐨勫寲瀛�(xu茅)淇＞鏂规硶
閰剁殑鐗╃悊淇＞
璀�(sh铆)瑷橈細閰剁殑鐗╃悊淇＞鐨勫畾缇╁強鍏剁壒榛�(di菐n)
绗簲绔� 閰惰垏绱�(x矛)鑳炲浐瀹氬寲
1. 閰跺拰鑿岄珨鍥哄畾鍖�
璀�(sh铆)瑷橈細 閰跺拰鑿岄珨鍥哄畾鍖栨柟娉曘€�
鐞嗚В锛� 鍥哄畾鍖栭叾鐨勬€ц唱(zh矛)
鎳�(y墨ng)鐢細 瀛�(xu茅)鏈�(hu矛)鍥哄畾鍖栭叾鐨勬噳(y墨ng)鐢�銆�
2. 寰敓鐗�銆佹鐗╁拰鍕�(d貌ng)鐗╃窗(x矛)鑳炲浐瀹氬寲
璀�(sh铆)瑷橈細 绱�(x矛)鑳炲浐瀹氬寲鐨勬柟娉�銆�
鎳�(y墨ng)鐢細 瀛�(xu茅)鏈�(hu矛)寰敓鐗�銆佹鐗┿€佸嫊(d貌ng)鐗╃窗(x矛)鑳炲浐瀹氬寲鐨勬噳(y墨ng)鐢�銆�
3. 鍘熺敓璩�(zh矛)楂斿浐瀹氬寲
璀�(sh铆)瑷橈細 鍘熺敓璩�(zh矛)楂斿埗鍌欏拰鍥哄畾鍖�銆�
鎳�(y墨ng)鐢細 鍥哄畾鍖栧師鐢熻唱(zh矛)楂旂殑鎳�(y墨ng)鐢ㄣ€�
绗叚绔� 閰惰垏鍥哄畾鍖栭叾鐨勫弽鎳�(y墨ng)鍕�(d貌ng)鍔涘(xu茅)
閰跺弽鎳�(y墨ng)鍕�(d貌ng)鍔涘(xu茅)
鐞嗚В锛歁ichaelis-Menten 蹇€熷钩琛″(xu茅)瑾強鍏舵剰缇�
鐞嗚В锛氬搴曠墿鍕�(d貌ng)鍔涘(xu茅)
鍚勭ó鍥犵礌灏�(du矛)閰跺弽鎳�(y墨ng)鍕�(d貌ng)鍔涘(xu茅)鐨勫奖闊�
鐞嗚В锛氬悇绋洜绱犲皪(du矛)閰跺弽鎳�(y墨ng)鍕�(d貌ng)鍔涘(xu茅)鐨勪笉鍚屽奖闊�
鍥哄畾鍖栭叾鍙嶆噳(y墨ng)鍕�(d貌ng)鍔涘(xu茅)
璀�(sh铆)瑷橈細鍥哄畾鍖栧皪(du矛)閰跺弽鎳�(y墨ng)绯荤当(t菕ng)鐨勫奖闊�
绗竷绔� 閰跺弽鎳�(y墨ng)鍣�
1. 閰跺弽鎳�(y墨ng)鍣ㄧ殑鐗归粸(di菐n)鑸囬鍨�
璀�(sh铆)瑷橈細 娓搁洟閰跺弽鎳�(y墨ng)鍣�銆佸浐瀹氬寲閰跺弽鎳�(y墨ng)鍣ㄣ€�
2. 閰跺弽鎳�(y墨ng)鍣ㄧ殑瑷�(sh猫)瑷�(j矛)鑸囬伕鍨�
鐞嗚В锛� 閰跺弽鎳�(y墨ng)鍣ㄧ殑瑷�(sh猫)瑷�(j矛)
鎳�(y墨ng)鐢細 閰跺弽鎳�(y墨ng)鍣ㄧ殑閬告搰
3. 閰跺弽鎳�(y墨ng)鍣ㄧ殑鎿嶄綔
鎳�(y墨ng)鐢細 閰跺弽鎳�(y墨ng)鍣ㄤ腑娴佸嫊(d貌ng)鐙€鎱�(t脿i)銆佹亽瀹氱敓鐢�(ch菐n)鑳藉姏銆佺┅(w臎n)瀹氭€т互鍙婂井鐢熺墿姹℃煋
鐨勬帶鍒�銆�
绗叓绔� 閰剁殑鎳�(y墨ng)鐢�
1. 閰跺湪杓曞伐銆侀鍝佹柟闈㈢殑鎳�(y墨ng)鐢�
璀�(sh铆)瑷橈細 閰跺湪瑭查牁(l菒ng)鍩熸噳(y墨ng)鐢�銆�
2. 閰跺湪閱�(y墨)钘ユ柟闈㈢殑鎳�(y墨ng)鐢�
璀�(sh铆)瑷橈細 閰跺湪瑭查牁(l菒ng)鍩熸噳(y墨ng)鐢�銆�
3. 閰跺湪鍒嗘瀽妾㈡脯鏂归潰鐨勬噳(y墨ng)鐢�
璀�(sh铆)瑷橈細 閰跺湪瑭查牁(l菒ng)鍩熸噳(y墨ng)鐢ㄣ€�
4. 閰跺湪鐢熺墿宸ョ▼涓噳(y墨ng)鐢�
璀�(sh铆)瑷橈細 閰跺湪瑭查牁(l菒ng)鍩熸噳(y墨ng)鐢�
鍥�銆佹暀鏉愬強鍙冭€冩浉鐩�
鏁� 鏉愶細閮媷涓荤法锛屻€婇叾宸ョ▼銆�锛屼腑鍦嬭紩宸ユキ(y猫)鍑虹増绀�锛�1994 骞�
鍙冭€冩浉鐩細缇呰泊姘戜富绶�锛屻€婇叾宸ョ▼銆嬶紝鍖栧(xu茅)宸ユキ(y猫)鍑虹増绀�锛�2002锛岀涓€鐗�
琚佸嫟鐢熶富绶�锛屻€婄従(xi脿n)浠ｉ叾瀛�(xu茅)銆�锛岃彲鏉辩悊宸ュぇ瀛�(xu茅)鍑虹増绀�锛�2001锛岀涓€鐗�
寮垫ü鏀夸富绶�锛屻€婇叾鍒跺姂宸ユキ(y猫)銆嬶紙涓娿€佷笅鍐�(c猫)锛�锛岀瀛�(xu茅)鍑虹増绀�锛�1998
闄崇煶鏍圭瓑钁�锛屻€婇叾瀛�(xu茅)銆�锛� 1987锛岀涓€鐗�
琚佸嫟鐢熺瓑钁楋紝銆婃噳(y墨ng)鐢ㄩ叾瀛�(xu茅)銆�锛�1994锛岀涓€鐗�
鎻�(y谩ng)闁嬪畤绛夎憲锛屻€婂熀鍥犺〃閬�(d谩)瑾�(di脿o)鎺ц垏鐢熺墿鎶€琛�(sh霉)涓殑閰跺(xu茅)銆�锛�1990锛岀涓€鐗�
鐜嬬拫锛屻€婇鍝侀叾瀛�(xu茅)銆�锛�1990锛岀涓€鐗�
Dixon M. and Webb E.D.: Enzymes (3rd Ed.), Longman, 1979.
鍏嶈铂(z茅)鑱叉槑锛氭湰鏂囩郴杞�(zhu菐n)杓夎嚜缍�(w菐ng)绲�(lu貌)锛屽鏈変镜鐘�锛岃珛(q菒ng)鑱�(li谩n)绯绘垜鍊戠珛鍗冲埅闄�锛屽彟锛氭湰鏂囧儏浠ｈ〃浣滆€呭€�(g猫)浜鸿榛�(di菐n)锛岃垏鏈恫(w菐ng)绔欑劇闂�(gu膩n)銆傚叾鍘熷壍(chu脿ng)鎬т互鍙婃枃涓櫝杩版枃瀛楀拰鍏�(n猫i)瀹规湭缍�(j墨ng)鏈珯璀夊(sh铆)锛屽皪(du矛)鏈枃浠ュ強鍏朵腑鍏ㄩ儴鎴栬€呴儴鍒嗗収(n猫i)瀹�銆佹枃瀛楃殑鐪熷(sh铆)鎬�銆佸畬鏁存€с€佸強鏅�(sh铆)鎬ф湰绔欎笉浣滀换浣曚繚璀夋垨鎵胯锛岃珛(q菒ng)璁€鑰呭儏浣滃弮鑰冿紝骞惰珛(q菒ng)鑷鏍稿(sh铆)鐩搁棞(gu膩n)鍏�(n猫i)瀹�銆�
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鎮ㄧ従(xi脿n)鍦ㄧ殑浣嶇疆锛� 
