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绗竴绔� 鍩哄洜宸ョ▼姒傝堪
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绗簩绔� 鍩哄洜宸ョ▼鐨勫伐鍏烽叾鍜岃級楂�
1.鍩哄洜宸ョ▼鐨勫伐鍏烽叾
鍩哄洜宸ョ▼鐨勫伐鍏烽叾鏈夊摢浜�锛熼檺鍒舵€�?x煤n)?n猫i)鍒囨牳閰搁叾鐨勭壒榛�(di菐n)鑸囦娇鐢ㄦ敞鎰�
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2. 鍩哄洜宸ョ▼鐨勮級楂�
鍩哄洜宸ョ▼杓夐珨蹇呴爤婊胯冻鐨勫熀鏈浠�锛岃唱(zh矛)绮掕級楂旂殑涓昏鐗瑰緛鑸囧熀鏈�
绲�(ji茅)妲�(g貌u)锛屽櫖鑿岄珨杓夐珨鏈夊摢浜涢(l猫i)鍨嬶紝鍏嬮殕杓夐珨鏈夊摢浜涢(l猫i)鍨�锛熸敎甯惰兘鍔涙湁澶�
澶э紵浜哄伐寰皬鏌撹壊楂旂殑姒傚康锛屾(bi膩o)瑷樺熀鍥犵殑绋(l猫i)銆備粈涔堟槸伪 浜掕(b菙)锛熻〃閬�(d谩)
杓夐珨鐨勬蹇佃垏鍩烘湰绲�(ji茅)妲�(g貌u)锛孴7 鍣弻楂斿暉鍕�(d貌ng)瀛愮郴绲�(t菕ng)鐨勭祫鎴�銆佽瀺鍚堣泲鐧借〃
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绗笁绔� 鐩殑鍩哄洜鐨勫埗鍌欒垏閼掑畾
1.鍩哄洜鏂囧韩(k霉)
鍩哄洜绲勬枃搴�(k霉)鐨勬蹇碉紝鍩哄洜绲勬枃搴�(k霉)鐨勬(g貌u)寤烘柟娉�锛� cDNA 鏂囧韩(k霉)鐨勬蹇�
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2.PCR 鎶€琛�(sh霉)
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3.铔嬬櫧璩�(zh矛)宸ョ▼
鍩哄洜绲勬暩(sh霉)鎿�(j霉)搴�(k霉)鐨勬蹇�锛岃泲鐧借唱(zh矛)宸ョ▼鐨勬蹇碉紝PCR 浠嬪皫(d菐o)瀹氶粸(di菐n)绐佽畩鐨�
鍘熺悊銆�
4. 鐩殑鍩哄洜閲嶇祫瀛愮殑閼掑畾
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绗洓绔� 鍩哄洜宸ョ▼鍦ㄥ熀鍥犲姛鑳界爺绌朵腑鐨勬噳(y墨ng)鐢�
1.鍩哄洜琛ㄩ仈(d谩)璀滅殑鐮旂┒
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2. 鍩哄洜绐佽畩鑸囧熀鍥犳暡闄�
杞�(zhu菐n)搴у瓙瑾樿畩鐨勫師鐞嗚垏鍎�(y艒u)榛�(di菐n)锛屽熀鍥犳暡闄ょ殑姒傚康鑸囨祦绋�锛屾浠跺熀鍥犳暡
闄ょ殑鍘熺悊鑸囧劒(y艒u)榛�(di菐n)锛孯NA 骞叉摼鐨勬蹇佃垏鍒嗗瓙姗�(j墨)鍒�锛孯NA 骞叉摼鐨勫劒(y艒u)榛�(di菐n)鑸囨噳(y墨ng)
鐢�?锛涱媰铚籢(gu貌)琛ㄩ仈(d谩)绯荤当(t菕ng)鐨勭祫鎴�锛孶AS-GAL4 绯荤当(t菕ng)鐨勭祫鎴愯垏鍎�(y艒u)榛�(di菐n)銆�
3.鍩哄洜鐨勭浉浜掍綔鐢ㄧ爺绌�
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绗簲绔� 杞�(zhu菐n)鍩哄洜妞嶇墿
1.杞�(zhu菐n)鍩哄洜妞嶇墿鐨勫埗鍌�
妞嶇墿杞�(zhu菐n)鍩哄洜鐨勫彈楂旂郴绲�(t菕ng)鐨勯(l猫i)鍨�锛屽婧愬熀鍥�?q奴)顬﹁儷鍙傞攺濮嗘菐?Ti
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2.杞�(zhu菐n)鍩哄洜妞嶇墿鐨勭従(xi脿n)鐙€
鎶楄煵(ch贸ng)杞�(zhu菐n)鍩哄洜妞嶇墿鐨勬蹇�锛屾姉闄よ崏鍔戣綁(zhu菐n)鍩哄洜浣滅墿鐨勬蹇�銆傛鐗╃敓鐗�
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绗叚绔� 杞�(zhu菐n)鍩哄洜鍕�(d貌ng)鐗�
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杞�(zhu菐n)閷勭梾姣掓硶鍒跺倷杞�(zhu菐n)鍩哄洜鍕�(d貌ng)鐗╃殑涓昏閬�(gu貌)绋�銆傝儦鑳庡共绱�(x矛)鑳炵殑姒傚康銆傝儦鑳庡共
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绗竷绔� 鍩哄洜娌荤檪
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鏂囩珷鎼滅储
鎮ㄧ従(xi脿n)鍦ㄧ殑浣嶇疆锛� 
