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鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�(gu贸)400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ(xu茅)鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗堬紝閮ㄥ垎瀛�(xu茅)鏍℃洿鏂拌嚦2012骞�锛�2013骞�锛涘潎鎻愪緵鏀惰不(f猫i)涓嬭級銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛�(di菐n)鎿娾€�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛�(di菐n)鎿娾€�鑰冨崥瑭﹀嵎搴�(k霉)鈥� 涓嬭級
婀栧崡甯寖澶у(xu茅)纰╁＋鐮旂┒鐢熷叆瀛�(xu茅)鑰冭│鑷懡椤岃€冭│澶х侗
鑰冭│绉戠洰浠ｇ⒓锛歔 ] 鑰冭│绉戠洰鍚嶇ū锛氱敓鐗╁寲瀛�(xu茅)
涓€銆佽│鍗风祼(ji茅)妲�(g貌u)
1) 瑭﹀嵎鎴愮妇(j墨)鍙婅€冭│鏅�(sh铆)闁�
鏈│鍗锋豢鍒嗙偤 100 鍒�锛岃€冭│鏅�(sh铆)闁撶偤 180 鍒嗛悩銆�
2)绛旈鏂瑰紡锛氶枆鍗�銆佺瓎瑭�
3)瑭﹀嵎鍏�(n猫i)瀹圭祼(ji茅)妲�(g貌u)
鐢熺墿鍖栧(xu茅) 100%
4)椤屽瀷绲�(ji茅)妲�(g貌u)
a: 鍚嶈瑙ｉ噵锛�10 灏忛锛屾瘡灏忛 4 鍒嗭紝鍏� 40 鍒�
b: 绨�(ji菐n)绛旈锛�6 灏忛锛屾瘡灏忛 6 鍒�锛屽叡 36 鍒�
c: 鍟忕瓟椤岋紝2 灏忛锛屾瘡灏忛 12 鍒嗭紝鍏� 24 鍒�
浜�銆佽€冭│鍏�(n猫i)瀹硅垏鑰冭│瑕佹眰
1銆佽泲鐧借唱(zh矛)鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳�
鑰冭│鍏�(n猫i)瀹�
铔嬬櫧璩�(zh矛)鐨勫垎瀛愮祫鎴� 绲勬垚浜洪珨鐨勮泲鐧借唱(zh矛)鐨� 20 绋皑鍩洪吀鐨勫垎椤炲強鍏剁悊鍖栨€ц唱(zh矛)
澶氳偨閺� 铔嬬櫧璩�(zh矛)鐨勫垎瀛愮祼(ji茅)妲�(g貌u) 铔嬬櫧璩�(zh矛)鐨勪竴绱�(j铆)绲�(ji茅)妲�(g貌u)銆佷簩绱�(j铆)绲�(ji茅)妲�(g貌u)銆佷笁绱�(j铆)绲�(ji茅)妲�(g貌u)鍜屽洓绱�(j铆)绲�(ji茅)
妲�(g貌u) 铔嬬櫧璩�(zh矛)绲勫(xu茅) 铔嬬櫧璩�(zh矛)鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳界殑闂�(gu膩n)绯� 铔嬬櫧璩�(zh矛)鐨勭悊鍖栨€ц唱(zh矛) 铔嬬櫧璩�(zh矛)鐨勮畩鎬�
铔嬬櫧璩�(zh矛)鐨勭传澶栧惛鏀� 铔嬬櫧璩�(zh矛)鐨勫惈閲忔脯(c猫)瀹� 铔嬬櫧璩�(zh矛)鐨勫垎闆㈢磾鍖栬垏绲�(ji茅)妲�(g貌u)鍒嗘瀽 閫忔瀽鍜�
瓒呮烤 铔嬬櫧璩�(zh矛)鐨勬矇娣€銆侀浕娉�銆佸堡鏋愬拰闆㈠績 铔嬬櫧璩�(zh矛)涓€绱�(j铆)绲�(ji茅)妲�(g貌u)鍜岄珮绱�(j铆)绲�(ji茅)妲�(g貌u)鐨勬脯(c猫)瀹�
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｈ泲鐧借唱(zh矛)鐨勬蹇�锛屾帉鎻¤泲鐧借唱(zh矛)鐨勫垎瀛愮祫鎴愬拰姘ㄥ熀閰哥殑鐞嗗寲鎬ц唱(zh矛)锛岀啛鎮�
姘ㄥ熀閰哥殑鍒嗛銆�
锛�2锛夋帉鎻¤泲鐧借唱(zh矛)涓€绱�(j铆)绲�(ji茅)妲�(g貌u)鍜岄珮绱�(j铆)绲�(ji茅)妲�(g貌u)鐨勬蹇碉紝鐔熸倝缍郴鍚勭礆(j铆)绲�(ji茅)妲�(g貌u)鐨勪富瑕佸寲瀛�(xu茅)
閸�銆�
锛�3锛夌悊瑙ｈ泲鐧借唱(zh矛)鐨勪竴绱�(j铆)绲�(ji茅)妲�(g貌u)鑸囬珮绱�(j铆)绲�(ji茅)妲�(g貌u)鍜屽姛鑳界殑闂�(gu膩n)绯讳互鍙婇珮绱�(j铆)绲�(ji茅)妲�(g貌u)鑸囧姛鑳界殑
闂�(gu膩n)绯�銆�
锛�4锛夌啛鎮夎泲鐧借唱(zh矛)绲勭殑姒傚康锛岀悊瑙ｈ泲鐧借唱(zh矛)绲勫(xu茅)鐨勭爺绌舵妧琛�(sh霉)銆�
锛�5锛夋帉鎻¤泲鐧借唱(zh矛)绛夐浕榛�(di菐n)鐨勬蹇碉紝鐞嗚В铔嬬櫧璩�(zh矛)鍏╂€цВ闆㈢殑鎬ц唱(zh矛)鍜岃啝楂旀€ц唱(zh矛)銆�
锛�6锛夋帉鎻¤泲鐧借唱(zh矛)璁婃€х殑瀹氱京銆佹湰璩�(zh矛)銆佺壒榛�(di菐n)鍜屼富瑕佺殑鎳�(y墨ng)鐢�銆�
锛�7锛夌悊瑙ｈ泲鐧借唱(zh矛)鐨勭传澶栧惛鏀剁壒鎬у拰铔嬬櫧璩�(zh矛)鐨勯洐绺劜鍙嶆噳(y墨ng)銆�.
锛�8锛夌悊瑙ｉ€忔瀽鍜岃秴婵剧殑姒傚康锛屾帉鎻¤泲鐧借唱(zh矛)娌夋穩鐨勬柟娉曞拰鍘熺悊銆�
锛�9锛夌悊瑙ｈ泲鐧借唱(zh矛)闆绘吵鐨勫師鐞嗗拰鍒嗛锛屼簡瑙ｅ叾涓昏鎳�(y墨ng)鐢�銆�
锛�10锛夌悊瑙ｈ泲鐧藉堡鏋愮殑鍘熺悊銆佸垎椤炲拰涓昏鎳�(y墨ng)鐢�銆�
锛�11锛変簡瑙ｈ泲鐧借唱(zh矛)搴忓垪鍒嗘瀽鍜岄珮绱�(j铆)绲�(ji茅)妲�(g貌u)娓�(c猫)瀹氱殑鏂规硶銆�
2銆佹牳閰哥殑绲�(ji茅)妲�(g貌u)鑸囧姛鑳�
鑰冭│鍏�(n猫i)瀹�
鏍搁吀鐨勭祫鎴愬強涓€绱�(j铆)绲�(ji茅)妲�(g貌u) DNA 鐨勭┖闁撶祼(ji茅)妲�(g貌u)鑸囧姛鑳� RNA 鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳� 鏍�
閰哥殑鐞嗗寲鎬ц唱(zh矛) 鏍搁吀閰躲€�
鑰冭│瑕佹眰
锛�1锛変簡瑙ｆ牳閰哥殑绲勬垚鍜屽垎椤�锛屾帉鎻℃牳閰镐竴绱�(j铆)绲�(ji茅)妲�(g貌u)鐨勬蹇点€�
锛�2锛夋帉鎻� DNA 鐨勪簩绱�(j铆)绲�(ji茅)妲�(g貌u)锛屼簡瑙� DNA 鐨勯珮绱�(j铆)绲�(ji茅)妲�(g貌u)锛岀悊瑙� DNA 鐨勫姛鑳�锛�
锛�3锛夋帉鎻� RNA 鐨勫垎椤炪€佺祼(ji茅)妲�(g貌u)鐗归粸(di菐n)鍙婂悇绋� RNA 鐨勫姛鑳�锛屼簡瑙ｆ牳閰稿湪鍘熸牳绱�(x矛)
鑳炲拰鐪熸牳绱�(x矛)鑳炰腑琛ㄧ従(xi脿n)鐨勪笉鍚屾檪(sh铆)绌虹壒鎬�銆�
锛�4锛夌悊瑙ｆ牳閰哥殑绱鍚告敹鐗规€э紝鎺屾彙 DNA 璁婃€х殑姒傚康銆佺壒榛�(di菐n)銆佸拰涓昏鎳�(y墨ng)
鐢�锛岀啛鎮夎畩鎬х殑涓昏鏂规硶锛岀悊瑙ｅ京(f霉)鎬у拰闆滀氦鐨勬蹇�銆�
锛�5锛夌悊瑙ｆ牳閰搁叾鐨勫垎椤炲拰浣滅敤鐗归粸(di菐n)銆�
3銆侀叾
鑰冭│鍏�(n猫i)瀹�
閰剁殑杓斿姪鍥犲瓙 閰剁殑娲绘€т腑蹇� 鍚屽伐閰� 閰朵績鍙嶆噳(y墨ng)鐨勭壒榛�(di菐n) 閰朵績鍙嶆噳(y墨ng)鐨勬(j墨)鐞�
鍚勭ó鍥犵礌灏�(du矛)閰朵績鍙嶆噳(y墨ng)閫熷害鐨勫奖闊� 閰舵椿鎬х殑瑾�(di脿o)绡€(ji茅) 閰剁殑鍛藉悕鑸囧垎椤� 閰跺拰鐤剧梾
鐨勯棞(gu膩n)绯� 閰跺湪閱�(y墨)瀛�(xu茅)涓婄殑鎳�(y墨ng)鐢�
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｉ叾鐨勬蹇�锛屼簡瑙ｉ叾鐨勮紨鍔╁洜瀛�銆�
锛�2锛夋帉鎻￠叾鐨勬椿鎬т腑蹇冨拰鍚屽伐閰剁殑姒傚康锛岀悊瑙ｅ悓宸ラ叾鐨勮嚚搴婃噳(y墨ng)鐢�銆�
锛�3锛夌悊瑙ｉ叾鍙嶆噳(y墨ng)鐨勭壒榛�(di菐n)鍜岄叾淇冨弽鎳�(y墨ng)鐨勬(j墨)鍒躲€�
锛�4锛夌悊瑙ｅ簳鐗╂績搴﹁垏閰朵績鍙嶆噳(y墨ng)閫熷害鐨勯棞(gu膩n)绯诲拰绫虫皬鏂圭▼锛屾帉鎻� Km 鍊�锛屽(xu茅)鏈�(hu矛)
浣滃湒娉曟眰鍙� Km 鍜� Vmax锛涗簡瑙ｉ叾婵冨害銆乸H 鍊�銆佹縺娲诲姂灏�(du矛)閰朵績鍙嶆噳(y墨ng)閫熷害鐨勫奖闊�銆�
锛�5锛夌悊瑙ｉ叾鐨勬姂鍒跺姂銆佸彲閫嗘姂鍒朵綔鐢ㄥ拰涓嶅彲閫嗘姂鍒朵綔鐢ㄧ殑姒傚康锛屼簡瑙ｆ湁姗�(j墨)
纾蜂腑姣掑拰閲嶉噾灞腑姣掔殑姗�(j墨)鍒�銆�
锛�6锛夋帉鎻＄(j矛ng)鐖�(zh膿ng)鎬ф姂鍒朵綔鐢ㄧ殑姒傚康銆佸嫊(d貌ng)鍔涘(xu茅)鐗归粸(di菐n)锛岀悊瑙ｇ：鑳洪钘ョ墿鐨勪綔鐢ㄦ(j墨)
鍒讹紝浜嗚В闈炵(j矛ng)鐖�(zh膿ng)鎬ф姂鍒朵綔鐢ㄥ拰鍙嶇(j矛ng)鐖�(zh膿ng)鎬ф姂鍒朵綔鐢ㄧ殑姒傚康鍜屽嫊(d貌ng)鍔涘(xu茅)鐗归粸(di菐n)銆�
锛�7锛夌悊瑙ｉ叾鐨勮畩妲�(g貌u)瑾�(di脿o)绡€(ji茅)銆佸叡鍍�(ji脿)淇＞瑾�(di脿o)绡€(ji茅)鍜岄叾鍘熺殑婵€娲�锛屼簡瑙ｉ叾鐨勮獦灏�(d菐o)鍜岄樆
閬忚(di脿o)绡€(ji茅)銆侀叾鐨勯檷瑙�锛岀啛鎮夐叾鐨勫懡鍚嶅拰鍒嗛銆�
锛�8锛夋帉鎻￠叾鑸囬啱(y墨)瀛�(xu茅)鐨勯棞(gu膩n)绯�锛岀啛鎮夐叾鍦ㄩ啱(y墨)瀛�(xu茅)涓婄殑鎳�(y墨ng)鐢ㄣ€�
4銆佺硸浠ｈ瑵
鑰冭│鍏�(n猫i)瀹�
绯栫殑鐢熺悊鍔熻兘 绯栫殑娑堝寲鍚告敹 绯栫殑鐒℃哀閰佃В 绯栫殑鏈夋哀姘у寲 绯栦唬璎濈殑
纾烽吀鎴婄硸閫斿緫 绯栧師鐨勫悎鎴愯垏鍒嗚В 绯栫暟鐢� 琛€绯栧強鍏惰(di脿o)绡€(ji茅) 琛€绯栫暟甯歌垏绯栧翱
鐥�
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｇ硸鐨勫畾缇�銆佺硸鐨勭敓鐞嗗姛鑳姐€佺硸鐨勬秷鍖栧惛鏀�銆�
锛�2锛夋帉鎻＄硸閰佃В鐨勫弽鎳�(y墨ng)閬庣▼鍙婂叾闂�(gu膩n)閸甸叾锛岀悊瑙ｇ硸閰佃В鐨勭敓鐞嗘剰缇┿€�
锛�3锛夋帉鎻′笝閰吀鑴晶銆佷笁缇ч吀寰挵(hu谩n)鐨勫弽鎳�(y墨ng)閬庣▼鍙婂叾闂�(gu膩n)閸甸叾锛岀悊瑙ｄ笁缇ч吀寰�
鐠�(hu谩n)鐨勬剰缇�锛岀悊瑙ｄ笁缇ч吀寰挵(hu谩n)涓� ATP 鐨勭敓鎴�銆�
锛�4锛夋帉鎻＄７閰告垔绯栭€斿緫鐨勫弽鎳�(y墨ng)閬庣▼闂�(gu膩n)閸甸叾鍙婂叾鐢熺悊鎰忕京銆�
锛�5锛夌悊瑙ｇ硸鍘熺殑鍚堟垚鍜屽垎瑙ｇ殑鍙嶆噳(y墨ng)姝ラ锛屾帉鎻＄硸鍘熷悎鎴愬拰鍒嗚В鐨勯棞(gu膩n)閸甸叾锛�
浜嗚В绯栧師绱⿳鐧ャ€�
锛�6锛夋帉鎻＄硸鐣扮敓鐨勬蹇�锛涚悊瑙ｇ硸鐣扮敓鐨勫弽鎳�(y墨ng)閬庣▼锛涗簡瑙ｇ硸鐣扮敓鐨勭敓鐞嗘剰缇�锛�
鐞嗚В涔抽吀寰挵(hu谩n)鍙婂叾鐢熺悊鎰忕京銆�
锛�7锛夌悊瑙ｈ绯栫殑姒傚康锛屾帉鎻¤绯栦締婧愯垏鍘昏矾锛岀悊瑙ｈ(di脿o)绡€(ji茅)琛€绯栫殑婵€绱犲強鍏朵綔
鐢ㄦ(j墨)鍒�锛涗簡瑙ｈ绯栧崌楂樺拰闄嶄綆鎵€鑷寸殑鐤剧梾銆�
5銆佽剛椤炰唬璎�
鑰冭│鍏�(n猫i)瀹�
鑴傞吀鐨勫懡鍚嶈垏鍒嗛 鑴傞鐨勬秷鍖栧惛鏀� 鐢樻补涓夐叝浠ｈ瑵 纾疯剛浠ｈ瑵 鑶藉浐閱�
浠ｈ瑵 琛€婕胯剛铔嬬櫧浠ｈ瑵
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｈ剛椤炵殑瀹氱京銆佷笉椋藉拰鑴傝偑閰哥殑鍛藉悕鑸囧垎椤烇紝鎺屾彙鐕�(y铆ng)椁�(y菐ng)蹇呴渶鑴傝偑閰�
鐨勫畾缇╁拰绋銆�.
锛�2锛変簡瑙ｈ剛椤炵殑娑堝寲鍜岃剛椤炵殑鍚告敹銆�
锛�3锛変簡鑴傞鐨勪富瑕佸劜(ch菙)瀛樺舰寮�锛岀悊瑙ｇ敇娌逛笁閰殑鐢熺悊鍔熻兘锛屾帉鎻¤剛鑲嫊(d貌ng)鍝＄殑
姒傚康鍙婂叾闂�(gu膩n)閸甸叾锛涚悊瑙ｈ剛瑙ｆ縺绱犲拰鎶楄剛瑙ｆ縺绱�锛涚悊瑙ｇ敇娌圭殑鍒嗚В浠ｈ瑵锛屾帉鎻¤剛閰�
鐨勎� -姘у寲鍒嗚В锛屾帉鎻￠叜楂旂殑鐢熸垚鑸囧埄鐢ㄤ互鍙婇叜楂旂敓鎴愮殑鐢熺悊鎰忕京锛岀悊瑙ｈ剛閰哥殑
鍚堟垚鍘熸枡鍜岄棞(gu膩n)閸甸叾锛岀啛鎮夎剛閰哥⒊閺堢殑寤堕暦(zh菐ng)锛岀悊瑙ｈ剛閰稿悎鎴愮殑瑾�(di脿o)绡€(ji茅)锛岀啛鎮夌敇娌逛笁閰�
鐨勫悎鎴愶紝浜嗚В鍓嶅垪鑵虹礌銆佽鏍撶兎銆佺櫧涓夌儻鐨勪富瑕佸姛鑳�銆�
锛�4锛夌悊瑙ｇ７鑴傜殑绲勬垚鍜岀７鑴傜殑鍔熻兘锛屼簡瑙ｇ敇娌圭７鑴傜殑鍚堟垚鑸囬檷瑙ｃ€�
锛�5锛夋帉鎻¤喗鍥洪唶鍚堟垚鐨勯儴浣�銆佸師鏂欏拰鍩烘湰鐨勫弽鎳�(y墨ng)閬庣▼锛屼簡瑙ｈ喗鍥洪唶鍚堟垚鐨�
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锛�6锛夌悊瑙ｈ鑴傜殑姒傚康锛屼簡瑙ｈ婕胯剛铔嬬櫧鐨勫垎椤烇紝鎺屾彙琛€婕胯剛铔嬬櫧鐨勫姛鑳�锛�
浜嗚В琛€婕胯剛铔嬬櫧浠ｈ瑵鐣板父鑸囬伜鍌冲拰鐤剧梾鐨勯棞(gu膩n)绯汇€�
6銆佺敓鐗╂哀鍖�
鑰冭│鍏�(n猫i)瀹�
姘у寲鍛煎惛閺� 姘у寲纾烽吀鍖� 绶氱矑楂斿収(n猫i)鑶滃皪(du矛)鐗╄唱(zh矛)鐨勯伕鎿囨€ц綁(zhu菐n)閬�(y霉n) 涓嶇敓鎴� ATP
鐨勬哀鍖栭珨绯�
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｇ敓鐗╂哀鍖栥€侀浕瀛愬偝閬為張鐨勬蹇�锛屾帉鎻￠浕瀛愬偝閬為張鐨勭祫鎴愬拰鎺掑垪闋�
搴忋€�
锛�2锛夋帉鎻℃哀鍖栫７閰稿寲鐨勬蹇�锛屼簡瑙ｆ哀鍖栫７閰稿寲鍋惰伅(li谩n)鐨勬(j墨)鍒�锛岀悊瑙ｅ奖闊挎哀鍖�
纾烽吀鍖栫殑鍥犵礌锛屼簡瑙� ATP 鍦ㄨ兘閲忕敓鎴�銆佸埄鐢ㄣ€佸劜(ch菙)瀛樺拰杞�(zhu菐n)绉讳腑鐨勪綔鐢�銆�.
锛�3锛夌悊瑙ｇ窔绮掗珨鍏�(n猫i)鑶滃皪(du矛)鍚勭ó鐗╄唱(zh矛)鐨勯伕鎿囨€ц綁(zhu菐n)绉�銆�
锛�4锛変簡瑙ｆ姉姘у寲閰堕珨绯诲拰寰矑楂旂窗(x矛)鑳炶壊绱� P450 鍠姞姘ч叾鐨勫姛鑳�銆�
7銆佹皑鍩洪吀浠ｈ瑵
鑰冭│鍏�(n猫i)瀹�
铔嬬櫧璩�(zh矛)鐨勭嚐(y铆ng)椁�(y菐ng)浣滅敤 姘钩琛″拰铔嬬櫧璩�(zh矛)鐨勭嚐(y铆ng)椁�(y菐ng)鍍�(ji脿)鍊� 铔嬬櫧璩�(zh矛)鐨勬秷鍖�銆佸惛鏀跺拰鑵�
鏁� 姘ㄥ熀閰哥殑鑴皑鍩轰綔鐢� 姘ㄥ熀閰哥⒊閺堥鏋剁殑杞�(zhu菐n)鎻涜垏鍒嗚В 楂斿収(n猫i)姘ㄧ殑渚嗘簮鑸囧幓璺�
灏跨礌鍚堟垚 鍊�(g猫)鍒ユ皑鍩洪吀鐨勪唬璎� 姘ㄥ熀閰哥殑鑴晶鍩轰綔鐢� 涓€纰冲柈浣嶇殑鐢熸垚鑸囧姛鑳�
鍚～姘ㄥ熀閰哥殑浠ｈ瑵 鑺抽鏃忔皑鍩洪吀浠ｈ瑵 鏀張姘ㄥ熀閰哥殑鍒嗚В
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｈ泲鐧借唱(zh矛)鐨勫姛鑳�锛屾帉鎻℃爱骞宠 銆佺嚐(y铆ng)椁�(y菐ng)蹇呴渶姘ㄥ熀閰搞€佽泲鐧借唱(zh矛)鐕�(y铆ng)椁�(y菐ng)鍍�(ji脿)鍊�
鐨勬蹇�銆�
锛�2锛夌悊瑙ｈ泲鐧借唱(zh矛)娑堝寲鐨勫牬(ch菐ng)鎵€鍜岄亷绋�銆佸惛鏀剁殑褰㈠紡鍜屼富瑕侀儴浣�锛屼簡瑙ｈ泲鐧借唱(zh矛)
鐨勮厫鏁椾綔鐢�銆�
锛�3锛夋帉鎻℃皑鍩洪吀鐨勮劔姘ㄥ熀浣滅敤锛岀悊瑙ｅ槍鍛ゆ牳鑻烽吀寰挵(hu谩n)锛屼簡瑙ｆ皑鍩洪吀纰抽張楠�
鏋剁殑浠ｈ瑵銆�
锛�4锛夋帉鎻￠珨鍏�(n猫i)姘ㄧ殑渚嗘簮鍜屾皑鐨勫幓璺紝鎺屾彙涓欐皑閰�-钁¤悇绯栧惊鐠�(hu谩n)鍙婂叾鎰忕京锛屼簡
瑙ｉ珨鍏�(n猫i)姘ㄧ殑杞�(zhu菐n)閬�(y霉n)锛屾帉鎻″翱绱犲悎鎴愮殑槌ユ皑閰稿惊鐠�(hu谩n)瀛�(xu茅)瑾�锛岀悊瑙ｅ翱绱犲悎鎴愮殑瑾�(di脿o)绡€(ji茅)銆�
锛�5锛夌悊瑙ｆ皑鍩洪吀鐨勮劔缇у熀浣滅敤锛屾帉鎻′竴纰冲柈浣嶇殑瀹氱京锛岀悊瑙ｄ竴纰冲柈浣嶇敓鎴�
鍙婂叾鍔熻兘銆�
锛�6锛夋帉鎻＄敳纭皑閰稿惊鐠�(hu谩n)锛岀悊瑙ｈ姵棣欐棌姘ㄥ熀閰哥殑浠ｈ瑵锛屼簡瑙ｆ敮閺堟皑鍩洪吀鐨勪唬
璎�銆�
8銆佹牳鑻烽吀浠ｈ瑵
鑰冭│鍏�(n猫i)瀹�
鏍搁吀鐨勬秷鍖栧拰鏍歌嫹閰哥殑鐢熺墿瀛�(xu茅)鍔熺敤 鍢屽懁鏍歌嫹閰哥殑鍚堟垚鑸囧垎瑙� 鍢屽懁鏍�
鑻烽吀鐨勭浉浜掕綁(zhu菐n)璁� 鑴哀鏍歌嫹閰哥殑鐢熸垚 鍢屽懁鏍歌嫹閰哥殑鎶椾唬璎濈墿 鍢у暥鏍歌嫹閰哥殑鍚�
鎴愯垏鍒嗚В 鍢у暥鏍歌嫹閰哥殑鎶椾唬璎濈墿
鑰冭│瑕佹眰
锛�1锛変簡瑙ｆ牳閰哥殑娑堝寲鍜屾牳鑻烽吀鐨勭敓鐗╁(xu茅)鍔熺敤銆�
锛�2锛夋帉鎻″槍鍛ゆ牳鑻烽吀鐨勫緸闋悎鎴愰€斿緫锛岀悊瑙ｅ槍鍛ゆ牳鑻烽吀鐨勮(b菙)鏁戝悎鎴愰€斿緫锛�
浜嗚В鍢屽懁鏍歌嫹閰哥殑鐩镐簰杞�(zhu菐n)璁�锛岀悊瑙ｈ劔姘ф牳鑻烽吀鐨勭敓鎴�銆�
锛�3锛夌悊瑙ｅ槍鍛ゆ牳鑻烽吀鐨勬姉浠ｈ瑵鐗╁強鍏舵噳(y墨ng)鐢紝鎺屾彙鍢屽懁鏍歌嫹閰哥殑鍒嗚В浠ｈ瑵銆�
锛�4锛夋帉鎻″槯鍟舵牳鑻烽吀鐨勫緸闋悎鎴愰€斿緫锛岀悊瑙ｅ槯鍟舵牳鑻烽吀鐨勮(b菙)鏁戝悎鎴愰€斿緫銆�
锛�5锛夌悊瑙ｅ槯鍟舵牳鑻烽吀鐨勬姉浠ｈ瑵鐗╁強鍏舵噳(y墨ng)鐢紟
锛�6锛変簡瑙ｅ槯鍟舵牳鑻烽吀鐨勫垎瑙ｄ唬璎�銆�
9銆佺墿璩�(zh矛)浠ｈ瑵鐨勮伅(li谩n)绯昏垏瑾�(di脿o)绡€(ji茅)
鑰冭│鍏�(n猫i)瀹�
顥ヮ棩鐗╄唱(zh矛)浠ｈ瑵鐨勭壒榛�(di菐n) 鐗╄唱(zh矛)浠ｈ瑵鐨勭浉浜掕伅(li谩n)绯� 楂斿収(n猫i)閲嶈绲勭箶銆佸櫒瀹樼殑浠ｈ瑵鐗归粸(di菐n)鍙�
鑱�(li谩n)绯� 浠ｈ瑵瑾�(di脿o)绡€(ji茅)鐨勬柟寮� 浠ｈ瑵绲勫(xu茅)
鑰冭│瑕佹眰
锛�1锛変簡瑙ｄ唬璎濊(di脿o)绡€(ji茅)鐨勭壒榛�(di菐n)銆�
锛�2锛夌悊瑙ｅ悇绋兘婧愮墿璩�(zh矛)鐨勪唬璎濊伅(li谩n)绯昏垏鍒剁磩锛屾帉鎻＄硸銆佽剛銆佽泲鐧借唱(zh矛)鐨勭浉浜掕伅(li谩n)
绯汇€�
锛�3锛夌悊瑙ｈ倽鍦ㄧ墿璩�(zh矛)浠ｈ瑵涓殑浣滅敤锛屼簡瑙ｅ績銆佽叇銆佽倢鑲�銆佺磪绱�(x矛)鑳炲皪(du矛)鑳芥簮鐗╄唱(zh矛)
鐨勫埄鐢�锛岀啛鎮夎剛鑲祫绻斿拰鑵庣殑鍔熻兘銆�
锛�4锛夌悊瑙ｇ窗(x矛)鑳炴按骞崇殑浠ｈ瑵瑾�(di脿o)绡€(ji茅)锛屼簡瑙ｆ縺绱犲皪(du矛)浠ｈ瑵閬庣▼鐨勮(di脿o)绡€(ji茅)锛岀啛鎮夌墿璩�(zh矛)浠�
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锛�5锛夌悊瑙ｄ唬璎濈祫瀛�(xu茅)鐨勬蹇�锛屼簡瑙ｄ唬璎濈祫瀛�(xu茅)鐨勬噳(y墨ng)鐢�銆�
10銆丏NA 鐢熺墿鍚堟垚
鑰冭│鍏�(n猫i)瀹�
顥ヮ棩顥ヤ腑蹇冩硶鍓� 寰�(f霉)鍒剁殑姒傚康 寰�(f霉)鍒剁殑鍩烘湰瑕�(gu墨)寰� DNA 寰�(f霉)鍒剁殑閰跺(xu茅)鍩虹(ch菙)鍜屾嫇
鎾插(xu茅)璁婂寲 DNA 鐢熺墿鍚堟垚鐨勯亷绋� 閫嗚綁(zhu菐n)閷� D 鐠�(hu谩n)寰�(f霉)鍒� DNA 鎼嶅偡鑸囦慨寰�(f霉)
鑰冭│瑕佹眰
顥ワ紙1锛夌悊瑙ｄ腑蹇冩硶鍓囩殑姒傚康銆�
锛�2锛夌悊瑙ｅ京(f霉)鍒剁殑姒傚康锛屾帉鎻″京(f霉)鍒剁殑鍩烘湰瑕�(gu墨)寰�锛屾帉鎻″崐淇濈暀寰�(f霉)鍒�銆侀洐鍚戝京(f霉)鍒�銆�
鍗婁笉閫ｇ簩(x霉)寰�(f霉)鍒剁殑姒傚康锛屼簡瑙ｅ崐淇濈暀 DNA 寰�(f霉)鍒剁殑瀵�(sh铆)椹�(y脿n)渚濇摎(j霉)銆�
锛�3锛夌悊瑙� DNA 寰�(f霉)鍒堕渶瑕佺殑鐢熺墿鍒嗗瓙鍜屽京(f霉)鍒剁殑鍩烘湰鍖栧(xu茅)鍙嶆噳(y墨ng)锛屾帉鎻″師鏍哥敓鐗�
鐨� DNA 鑱氬悎閰跺強鍏舵椿鎬х壒榛�(di菐n)锛屾帉鎻＄湡鏍哥敓鐗╃殑 DNA 鑱氬悎閰跺強鍏舵椿鎬х壒榛�(di菐n)锛岀悊
瑙ｅ京(f霉)鍒朵繚鐪熸€х殑閰跺(xu茅)渚濇摎(j霉)锛屼簡瑙ｅ京(f霉)鍒朵腑 DNA 鍒嗗瓙鐨勬嫇?f霉)閶礧(xu茅)璁婂寲锛岀悊瑙� DNA 閫�
鎺ラ叾鍙婂叾浣滅敤鐗归粸(di菐n)銆�
锛�4锛夋帉鎻″師鏍哥敓鐗� DNA 鐢熺墿鍚堟垚鐨勯亷绋�锛岀悊瑙ｇ湡鏍哥敓鐗╃殑 DNA 鐢熺墿鍚堟垚锛�
鎺屾彙绔矑鐨勬蹇靛強鍏剁敓鐗╁(xu茅)鍔熻兘锛岀悊瑙ｄ汉椤炵殑绔矑閰跺強鍏舵椿鎬х壒榛�(di菐n)銆�
锛�5锛夋帉鎻￠€嗚綁(zhu菐n)閷勭殑姒傚康锛岀悊瑙ｉ€嗚綁(zhu菐n)閷勭殑閬庣▼锛屼簡瑙ｇ櫦(f膩)鐝�(xi脿n)閫嗚綁(zhu菐n)閷勭殑鐢熺墿瀛�(xu茅)鎰忕京銆�
鐔熸倝 D 鐠�(hu谩n)寰�(f霉)鍒�銆�
锛�6锛夌悊瑙ｇ獊璁婄殑鐢熺墿瀛�(xu茅)鎰忕京锛屼簡瑙ｅ紩璧风獊璁婄殑鐗╃悊銆佸寲瀛�(xu茅)鍥犵礌锛屾帉鎻＄獊璁婄殑
椤炲瀷锛岀悊瑙� DNA 鎼嶅偡淇京(f霉)鐨勪富瑕侀鍨�銆�
11銆丷NA 鐨勭敓鐗╁悎鎴�
鑰冭│鍏�(n猫i)瀹�
杞�(zhu菐n)閷勭殑姒傚康 寰�(f霉)鍒惰垏杞�(zhu菐n)閷勭殑鐩稿悓榛�(di菐n)鍜屼笉鍚岄粸(di菐n) 杞�(zhu菐n)閷勭殑妯℃澘鍜岄叾 鍘熸牳鐢熺墿
鐨勮綁(zhu菐n)閷勯亷绋� 鐪熸牳鐢熺墿 RNA 鐨勭敓鐗╁悎鎴� 鐪熸牳鐢熺墿 RNA 鐨勫姞宸�
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｈ綁(zhu菐n)閷勭殑姒傚康锛屾帉鎻″京(f霉)鍒跺拰杞�(zhu菐n)閷勭殑鐩稿悓榛�(di菐n)鍜屼笉鍚岄粸(di菐n)銆�
锛�2锛夌悊瑙ｇ祼(ji茅)妲�(g貌u)鍩哄洜銆佷笉灏�(du矛)绋辫綁(zhu菐n)闄哥殑姒傚康锛屾帉鎻″師鏍哥敓鐗� RNA 鑱氬悎閰剁殑绲�
鎴�銆佸悇浜炲熀鐨勫姛鑳藉拰閰剁殑娲绘€х壒榛�(di菐n)锛岀悊瑙� RNA 鑱氬悎閰惰垏 DNA 鍟熷嫊(d貌ng)瀛愮殑绲�(ji茅)鍚堝強
RNA 鑱氬悎閰跺偓鍖栫殑 RNA 鐢熺墿鍚堟垚銆�
锛�3锛夋帉鎻″師鏍哥敓鐗╃殑杞�(zhu菐n)閷勯亷绋�锛屼簡瑙ｈ綁(zhu菐n)閷勫欢闀�(zh菐ng)鍜岀炕璀悓鏅�(sh铆)閫�(j矛n)琛岀殑鐝�(xi脿n)璞★紝鐞�
瑙ｄ緷璩聪� 鍥犲瓙鐨勮綁(zhu菐n)閷勭祩姝㈠拰涓嶄緷璩聪� 鍥犲瓙鐨勮綁(zhu菐n)閷勭祩姝�銆�
锛�4锛夌悊瑙ｇ湡鏍哥敓鐗╃殑 RNA 鑱氬悎閰剁殑绋鍜屽姛鑳�锛屾帉鎻￠爢寮忎綔鐢ㄥ厓浠�銆佸弽
寮忎綔鐢ㄥ洜瀛愩€佽綁(zhu菐n)閷勫洜瀛愮殑鐨勬蹇�锛岀悊瑙ｇ湡鏍哥敓鐗╃殑杞�(zhu菐n)閷勮捣濮嬮亷绋嬨€佸悇绋綁(zhu菐n)閷勫洜瀛�
鐨勪綔鐢�锛屼簡瑙ｆ嫾鏉跨悊璜栧拰杞�(zhu菐n)閷勫欢闀�(zh菐ng)鐨勭壒榛�(di菐n)锛岀啛鎮夌湡鏍哥敓鐗╄綁(zhu菐n)閷勭祩姝㈢殑閬庣▼銆�
锛�5锛夋帉鎻＄湡鏍哥敓鐗� mRNA 鐨勮綁(zhu菐n)閷勫姞宸ラ亷绋嬶紝鐞嗚В鏂疯鍩哄洜銆佸椤瓙銆佸収(n猫i)鍚�
瀛愮殑姒傚康锛岀悊瑙� mRNA 鐨勫壀鎺�銆�
锛�6锛変簡瑙� rRNA銆乼RNA 鐨勫姞宸ラ亷绋嬪拰 RNA 鍌寲鐨勮嚜鎴戝壀鎺�銆�
12銆佽泲鐧借唱(zh矛)鐨勭敓鐗╁悎鎴�
鑰冭│鍏�(n猫i)瀹�:
缈昏 铔嬬櫧璩�(zh矛)鐢熺墿鍚堟垚楂旂郴 閬哄偝瀵嗙⒓鐨勭壒榛�(di菐n) 姘ㄥ熀閰哥殑娲诲寲 鍘熸牳鐢熺墿
鐨勮偨閺堝悎鎴愰亷绋� 鐪熸牳鐢熺墿鐨勮偨閺堝悎鎴愰亷绋� 铔嬬櫧璩�(zh矛)缈昏鍚庣殑淇＞ 铔嬬櫧璩�(zh矛)鐨勯澏
鍚戣几閫� 铔嬬櫧璩�(zh矛)鐢熺墿鍚堟垚鐨勫共鎿惧拰鎶戝埗
鑰冭│瑕佹眰
锛�1锛夋帉鎻＄炕璀殑姒傚康锛�
锛�2锛夌悊瑙ｄ笁绋� RNA 鍦ㄨ泲鐧借唱(zh矛)鐢熺墿鍚堟垚涓殑浣滅敤銆�
锛�3锛夌悊瑙ｉ枊鏀鹃柋璁€妗嗘灦鐨勬蹇�锛屾帉鎻￠伜鍌冲瘑纰肩殑鐗归粸(di菐n)锛屼簡瑙ｈ泲鐧借唱(zh矛)鐢熺墿鍚�
鎴愰渶瑕佺殑閰堕鍜岃泲鐧借唱(zh矛)鍥犲瓙銆�
锛�4锛夋帉鎻℃皑鍩洪吀娲诲寲鐨勯亷绋嬪強姘ㄥ熀閰�-tRNA 鍚堟垚閰跺湪姘ㄥ熀閰歌垏 tRNA 姝ｇ⒑
绲�(ji茅)鍚堜腑鐨勪綔鐢�锛岀悊瑙ｈ捣濮嬫皑鍩洪叞-tRNA 鍚堟垚銆�
锛�5锛夋帉鎻″師鏍哥敓鐗╃殑鑲介張鍚堟垚閬庣▼鍙婂悇绋泲鐧借唱(zh矛)鍥犲瓙鐨勪綔鐢�锛屽(xu茅)鏈�(hu矛)瑷�(j矛)绠楄泲
鐧借唱(zh矛)鍚堟垚涓兘閲忕殑娑堣€�锛岀悊瑙ｇ湡鏍哥敓鐗╃殑鑲介張鍚堟垚閬庣▼锛屾瘮杓冨畠鍊戠殑涓昏宸垾銆�
锛�6锛夋帉鎻″垎瀛愪即渚剁殑姒傚康锛屼簡瑙ｅ鑲介張鎶樼枈褰㈡垚澶╃劧妲�(g貌u)鍍忕殑铔嬬櫧璩�(zh矛)锛岀悊瑙�
鑲介張鐨勬按瑙ｅ拰鍖栧(xu茅)淇＞锛屼簡瑙ｄ簽鍩虹殑鑱氬悎鍜岃紨鍩虹殑閫ｆ帴銆�
锛�7锛変簡瑙ｈ泲鐧借唱(zh矛)鐨勯澏鍚戣几閫�锛岀悊瑙ｄ俊铏�(h脿o)搴忓垪銆佷俊铏�(h脿o)鑲�銆佹牳瀹氫綅搴忓垪鐨勬蹇�銆�
锛�8锛夌悊瑙ｆ姉鐢熺礌鐨勬蹇�锛屾帉鎻′富瑕佹姉鐢熺礌鎶戝埗铔嬬櫧璩�(zh矛)鐢熺墿鍚堟垚鐨勫師鐞�锛屼簡
瑙ｆ瘨绱犲拰骞叉摼绱犵殑浣滅敤銆�
13銆佸熀鍥犺〃閬�(d谩)瑾�(di脿o)鎺�
鑰冭│鍏�(n猫i)瀹�:
鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺х殑鍩烘湰姒傚康 鍩哄洜琛ㄩ仈(d谩)鐨勬檪(sh铆)闁撳拰绌洪枔鐗圭暟鎬� 鍩哄洜琛ㄩ仈(d谩)鐨勬柟
寮� 鍩哄洜琛ㄩ仈(d谩)鐨勭敓鐗╁(xu茅)鎰忕京 鍩哄洜琛ㄩ仈(d谩)鐨勫熀鏈師鐞� 鍘熸牳鐢熺墿鍩哄洜杞�(zhu菐n)閷勮(di脿o)绡€(ji茅)鐗归粸(di菐n)
鍘熸牳鐢熺墿鍩哄洜杞�(zhu菐n)閷勮(di脿o)绡€(ji茅)鐨勬搷绺卞瓙姗�(j墨)鍒� 鍘熸牳鐢熺墿杞�(zhu菐n)閷勭祩姝㈢殑瑾�(di脿o)绡€(ji茅)姗�(j墨)鍒� 鍘熸牳鐢�
鐗╃炕璀按骞崇殑瑾�(di脿o)绡€(ji茅) 鐪熸牳鐢熺墿鍩哄洜绲勭殑绲�(ji茅)妲�(g貌u)鐗归粸(di菐n) 鐪熸牳鐢熺墿鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺х殑鐗归粸(di菐n)
RNA 鑱氬悎閰垛叀?q奴)顬扗(zhu菐n)閷勮捣濮嬬殑瑾�(di脿o)绡€(ji茅) 鐪熸牳鐢熺墿杞�(zhu菐n)閷勫悗姘村钩鐨勮(di脿o)绡€(ji茅) 鐪熸牳鐢熺墿缈昏
姘村钩鐨勮(di脿o)绡€(ji茅) 灏忓垎瀛� RNA 灏�(du矛)鍩哄洜琛ㄩ仈(d谩)鐨勮(di脿o)绡€(ji茅)
鑰冭│瑕佹眰:
锛�1锛夌悊瑙ｅ熀鍥犵祫銆佸熀鍥犺〃閬�(d谩)鐨勬蹇�锛屾帉鎻″熀鍥犺〃閬�(d谩)鐨勬檪(sh铆)闁撳拰绌洪枔鐗圭暟鎬э紝
鐞嗚В鍩烘湰琛ㄩ仈(d谩)銆佽獦灏�(d菐o)鍜岄樆閬忚〃閬�(d谩)锛屾帉鎻″熀鍥犺〃閬�(d谩)鐨勭敓鐗╁(xu茅)鎰忕京銆�
锛�2锛夌悊瑙ｅ熀鍥犺〃閬�(d谩)瑾�(di脿o)鎺х殑鍩烘湰鍘熺悊锛屾帉鎻″熀鍥犺綁(zhu菐n)閷勬縺娲荤殑鍩烘湰瑕佺礌銆�
锛�3锛夌悊瑙ｅ師鏍哥敓鐗╁熀鍥犺綁(zhu菐n)閷勮(di脿o)绡€(ji茅)鐨勭壒榛�(di菐n)锛屾帉鎻″師鏍哥敓鐗╁熀鍥犺綁(zhu菐n)閷勮捣濮嬭(di脿o)绡€(ji茅)
鐨勬搷绺卞瓙姗�(j墨)鍒�锛岀悊瑙ｅ師鏍哥敓鐗╀笉鍚岀殑杞�(zhu菐n)閷勭祩姝㈣(di脿o)绡€(ji茅)姗�(j墨)鍒�锛屼簡瑙ｅ師鏍哥敓鐗╃炕璀按骞�
鐨勮(di脿o)绡€(ji茅)銆�
锛�4锛夌悊瑙ｇ湡鏍哥敓鐗╁熀鍥犵祫鐨勭祼(ji茅)妲�(g貌u)鐗归粸(di菐n)鍜屽熀鍥犺〃閬�(d谩)瑾�(di脿o)鎺х殑鐗归粸(di菐n)锛屼簡瑙� RNA
鑱氬悎閰垛厾鍜� RNA 鑱氬悎閰垛參杞�(zhu菐n)閷勯珨绯荤殑瑾�(di脿o)绡€(ji茅)锛屾帉鎻� RNA 鑱氬悎閰垛叀鍙冭垏鐨勮綁(zhu菐n)閷勮捣濮�
鐨勮(di脿o)绡€(ji茅)锛屾帉鎻″暉鍕�(d貌ng)瀛�銆佸寮�(qi谩ng)瀛�銆佹矇榛樺瓙鐨勬蹇碉紝浜嗚В杞�(zhu菐n)閷勮(di脿o)绡€(ji茅)鍥犲瓙鐨勭祼(ji茅)妲�(g貌u)銆�
锛�5锛変簡瑙ｇ湡鏍哥敓鐗╄綁(zhu菐n)閷勫悗姘村钩鐨勮(di脿o)绡€(ji茅)鍜岀炕璀按骞崇殑瑾�(di脿o)绡€(ji茅)銆�
锛�7锛夌悊瑙ｅ皬鍒嗗瓙 RNA 灏�(du矛)鍩哄洜琛ㄩ仈(d谩)鐨勮(di脿o)绡€(ji茅)锛岀悊瑙ｅ井灏� RNA 鍜屽皬骞叉摼 RNA 鐨勬
蹇�銆�
14銆佸熀鍥犻噸绲勮垏鍩哄洜宸ョ▼
鑰冭│鍏�(n猫i)瀹�
鍚屾簮閲嶇祫 閲嶇祫 DNA 鎶€琛�(sh霉)鐨勭浉闂�(gu膩n)姒傚康 閲嶇祫 DNA 鎶€琛�(sh霉)鐨勫熀鏈師鐞� 閲嶇祫
DNA 鎶€琛�(sh霉)鑸囬啱(y墨)瀛�(xu茅)鐨勯棞(gu膩n)绯�
鑰冭│瑕佹眰
锛�1锛夋帉鎻″悓婧愰噸绲勭殑姒傚康锛屼簡瑙ｄ綅榛�(di菐n)鐗圭暟鎬ч噸绲勫拰杞�(zhu菐n)搴ч噸绲勩€�
锛�2锛夋帉鎻￠噸绲� DNA 鎶€琛�(sh霉)鐨勫熀鏈蹇�锛岀悊瑙ｉ噸绲� DNA 鎶€琛�(sh霉)鐨勫伐鍏烽叾鍜岃級楂�锛�
鎺屾彙閲嶇祫 DNA 鎶€琛�(sh霉)鐨勫熀鏈師鐞嗗拰鎿嶄綔姝ラ锛岀悊瑙ｇ嵅鍙栫洰鐨勫熀鍥犵殑鏂规硶锛岀悊瑙ｉ噸
绲� DNA 灏�(d菐o)鍏ュ涓荤窗(x矛)鑳炵殑鏂规硶锛屼簡瑙ｉ噸绲勬彁绡╅伕鐨勬柟娉�銆�
锛�3锛夌悊瑙ｉ噸绲� DNA 鎶€琛�(sh霉)鍦ㄩ啱(y墨)瀛�(xu茅)涓婄殑鎳�(y墨ng)鐢ㄣ€�
15銆佺窗(x矛)鑳炰俊铏�(h脿o)杞�(zhu菐n)灏�(d菐o)
鑰冭│鍏�(n猫i)瀹�
淇¤櫉(h脿o)杞�(zhu菐n)灏�(d菐o)鐨勫畾缇� 绱�(x矛)鑳炰俊铏�(h脿o)杞�(zhu菐n)灏�(d菐o)鐨勫熀鏈矾绶� 绱�(x矛)鑳炲鍖栧(xu茅)淇¤櫉(h脿o) 鍙楅珨鐨勫畾缇�銆�
鐗归粸(di菐n)鍜屽垎甯� 绱�(x矛)鑳炶綁(zhu菐n)灏�(d菐o)淇¤櫉(h脿o)鐨勬柟寮� 绱�(x矛)鑳炲収(n猫i)淇¤櫉(h脿o)杞�(zhu菐n)灏�(d菐o)鐩搁棞(gu膩n)鐨勫垎瀛� 鍩烘湰淇¤櫉(h脿o)杞�(zhu菐n)灏�(d菐o)閫�
璺� 淇¤櫉(h脿o)杞�(zhu菐n)灏�(d菐o)閬庣▼鐨勮(gu墨)寰嬭垏鐗归粸(di菐n) 绱�(x矛)鑳炰俊铏�(h脿o)杞�(zhu菐n)灏�(d菐o)鑸囬啱(y墨)瀛�(xu茅)
鑰冭│瑕佹眰
锛�1锛夋帉鎻′俊铏�(h脿o)杞�(zhu菐n)灏�(d菐o)鐨勬蹇碉紝鐞嗚В绱�(x矛)鑳炰俊铏�(h脿o)杞�(zhu菐n)灏�(d菐o)鐨勫熀鏈矾绶氬拰鏂瑰紡锛屼簡瑙ｇ窗(x矛)
鑳炲鍖栧(xu茅)淇¤櫉(h脿o)銆�
锛�2锛夋帉鎻″彈楂旂殑姒傚康鍜岀壒榛�(di菐n)锛岀悊瑙ｅ彈楂旂殑鍒嗗竷锛屼簡瑙ｄ俊铏�(h脿o)杞�(zhu菐n)灏�(d菐o)閫氳矾鍙婄窗(x矛)鑳�
杞�(zhu菐n)鍒颁俊铏�(h脿o)鐨勫熀鏈柟寮�銆�
锛�3锛夋帉鎻＄窗(x矛)鑳炲収(n猫i)閲嶈鐨勪簩绱�(j铆)淇′娇锛岀悊瑙ｄ簩绱�(j铆)淇′娇鐨勭敓鎴�锛屼簡瑙ｄ簩绱�(j铆)淇′娇鍙�
鑸囪(di脿o)绡€(ji茅)鐨勯澏鍒嗗瓙銆�
锛�4锛夌悊瑙ｇ窗(x矛)鑳炲収(n猫i)鐨勪俊铏�(h脿o)杞�(zhu菐n)灏�(d菐o)鍒嗗瓙銆�
锛�5锛夋帉鎻＄窗(x矛)鑳炲収(n猫i)鍙楅珨浠嬪皫(d菐o)鐨勪俊铏�(h脿o)杞�(zhu菐n)灏�(d菐o)閫斿緫锛岀悊瑙ｉ洟瀛愰€氶亾鍨嬪彈楂斾粙灏�(d菐o)鐨勪俊
铏�(h脿o)杞�(zhu菐n)灏�(d菐o)閫斿緫锛屾帉鎻′竷璺ㄨ啘鍙楅珨浠嬪皫(d菐o)鐨勪俊铏�(h脿o)杞�(zhu菐n)灏�(d菐o)閫斿緫锛岀悊瑙ｅ柈璺ㄨ啘鍙楅珨浠嬪皫(d菐o)鐨勪俊铏�(h脿o)
杞�(zhu菐n)灏�(d菐o)閫斿緫锛屼簡瑙ｄ俊铏�(h脿o)杞�(zhu菐n)灏�(d菐o)閬庣▼鐨勭壒榛�(di菐n)鑸囪(gu墨)寰�銆�
锛�6锛変簡瑙ｄ俊铏�(h脿o)杞�(zhu菐n)灏�(d菐o)鑸囬啱(y墨)瀛�(xu茅)鐨勯棞(gu膩n)绯汇€�
16銆佽娑茬殑鐢熺墿鍖栧(xu茅)
鑰冭│鍏�(n猫i)瀹�
琛€婕胯泲鐧界殑鍒嗛鑸囨€ц唱(zh矛) 琛€婕胯泲鐧借唱(zh矛)鐨勫姛鑳� 绱呯窗(x矛)鑳炵殑浠ｈ瑵鐗归粸(di菐n) 鐧界窗(x矛)鑳�
鐨勪唬璎�
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｈ婕胯泲鐧借唱(zh矛)鐨勫垎椤炲拰鎬ц唱(zh矛)锛屾帉鎻¤婕胯泲鐧借唱(zh矛)鐨勫姛鑳�銆�
锛�2锛夌悊瑙ｇ磪绱�(x矛)鑳炵硸浠ｈ瑵鐨勪富瑕侀€斿緫锛屼簡瑙� 2锛�3-浜岀７閰哥敇娌归吀鏃佽矾锛屾帉鎻＄磪
绱�(x矛)鑳炲収(n猫i)绯栦唬璎濈殑鐢熺悊鎰忕京銆�
锛�3锛夋帉鎻¤绱呯礌鐨勭敓鐗╁悎鎴愬強鍏惰(di脿o)绡€(ji茅)锛岀悊瑙ｈ绱呰泲鐧界殑鍚堟垚銆�
锛�4锛夌悊瑙ｇ櫧绱�(x矛)鑳炵殑浠ｈ瑵銆�
17銆佽倽鐨勭敓鐗╁寲瀛�(xu茅)
鑰冭│鍏�(n猫i)瀹�
鑲濆湪鐗╄唱(zh矛)浠ｈ瑵涓殑浣滅敤 鑲濈殑鐢熺墿杞�(zhu菐n)鍖栦綔鐢� 鑶芥眮鑸囪喗姹侀吀浠ｈ瑵 鑶借壊绱�
鐨勪唬璎濊垏榛冪柛
鑰冭│瑕佹眰
锛�1锛変簡瑙ｈ倽鍦ㄧ硸銆佽剛銆佽泲鐧借唱(zh矛)銆佺董鐢熺礌鍜屾縺绱犱唬璎濅腑鐨勪綔鐢ㄣ€�
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鑰冭│瑕佹眰
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涓�銆佸弮鑰冩浉鐩�
[1] 鏌婚尗鑹富绶�. 鐢熺墿鍖栧(xu茅)锛堢涓冪増锛�. 浜烘皯琛�(w猫i)鐢熷嚭鐗堢ぞ, 2008
[2] 鍔夌兢鑹富绶�. 鐢熺墿鍖栧(xu茅). 鍖栧(xu茅)宸ユキ(y猫)鍑虹増绀�, 2011
鍏嶈铂(z茅)鑱叉槑锛氭湰鏂囩郴杞�(zhu菐n)杓夎嚜缍�(w菐ng)绲�(lu貌)锛屽鏈変镜鐘�锛岃珛(q菒ng)鑱�(li谩n)绯绘垜鍊戠珛鍗冲埅闄�锛屽彟锛氭湰鏂囧儏浠ｈ〃浣滆€呭€�(g猫)浜鸿榛�(di菐n)锛岃垏鏈恫(w菐ng)绔欑劇闂�(gu膩n)銆傚叾鍘熷壍(chu脿ng)鎬т互鍙婃枃涓櫝杩版枃瀛楀拰鍏�(n猫i)瀹规湭缍�(j墨ng)鏈珯璀夊(sh铆)锛屽皪(du矛)鏈枃浠ュ強鍏朵腑鍏ㄩ儴鎴栬€呴儴鍒嗗収(n猫i)瀹�銆佹枃瀛楃殑鐪熷(sh铆)鎬�銆佸畬鏁存€�銆佸強鏅�(sh铆)鎬ф湰绔欎笉浣滀换浣曚繚璀夋垨鎵胯锛岃珛(q菒ng)璁€鑰呭儏浣滃弮鑰冿紝骞惰珛(q菒ng)鑷鏍稿(sh铆)鐩搁棞(gu膩n)鍏�(n猫i)瀹�銆�
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鏂囩珷鎼滅储
鎮ㄧ従(xi脿n)鍦ㄧ殑浣嶇疆锛� 
