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鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ(xu茅)鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗堬紝閮ㄥ垎瀛�(xu茅)鏍℃洿鏂拌嚦2012骞�锛�2013骞达紱鍧囨彁渚涙敹璨讳笅杓�銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛炴搳鈥�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛炴搳鈥�鑰冨崥瑭﹀嵎搴�鈥� 涓嬭級
婀栧崡甯寖澶у(xu茅)纰╁＋鐮旂┒鐢熷叆瀛�(xu茅)鑰冭│鑷懡椤岃€冭│澶х侗
鑰冭│绉戠洰浠ｇ⒓锛歔 ] 鑰冭│绉戠洰鍚嶇ū锛氱敓鐗╁寲瀛�(xu茅)
涓€銆佽│鍗风祼(ji茅)妲�(g貌u)
1) 瑭﹀嵎鎴愮妇鍙婅€冭│鏅傞枔
鏈│鍗锋豢鍒嗙偤 100 鍒�锛岃€冭│鏅傞枔鐐� 180 鍒嗛悩銆�
2)绛旈鏂瑰紡锛氶枆鍗�銆佺瓎瑭�
3)瑭﹀嵎鍏�(n猫i)瀹圭祼(ji茅)妲�(g貌u)
鐢熺墿鍖栧(xu茅) 100%
4)椤屽瀷绲�(ji茅)妲�(g貌u)
a: 鍚嶈瑙ｉ噵锛�10 灏忛锛屾瘡灏忛 4 鍒嗭紝鍏� 40 鍒�
b: 绨＄瓟椤�锛�6 灏忛锛屾瘡灏忛 6 鍒嗭紝鍏� 36 鍒�
c: 鍟忕瓟椤�锛�2 灏忛锛屾瘡灏忛 12 鍒嗭紝鍏� 24 鍒�
浜�銆佽€冭│鍏�(n猫i)瀹硅垏鑰冭│瑕佹眰
1銆佽泲鐧借唱(zh矛)鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳�
鑰冭│鍏�(n猫i)瀹�
铔嬬櫧璩�(zh矛)鐨勫垎瀛愮祫鎴� 绲勬垚浜洪珨鐨勮泲鐧借唱(zh矛)鐨� 20 绋皑鍩洪吀鐨勫垎椤炲強鍏剁悊鍖栨€ц唱(zh矛)
澶氳偨閺� 铔嬬櫧璩�(zh矛)鐨勫垎瀛愮祼(ji茅)妲�(g貌u) 铔嬬櫧璩�(zh矛)鐨勪竴绱氱祼(ji茅)妲�(g貌u)銆佷簩绱氱祼(ji茅)妲�(g貌u)銆佷笁绱氱祼(ji茅)妲�(g貌u)鍜屽洓绱氱祼(ji茅)
妲�(g貌u) 铔嬬櫧璩�(zh矛)绲勫(xu茅) 铔嬬櫧璩�(zh矛)鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳界殑闂�(gu膩n)绯� 铔嬬櫧璩�(zh矛)鐨勭悊鍖栨€ц唱(zh矛) 铔嬬櫧璩�(zh矛)鐨勮畩鎬�
铔嬬櫧璩�(zh矛)鐨勭传澶栧惛鏀� 铔嬬櫧璩�(zh矛)鐨勫惈閲忔脯瀹� 铔嬬櫧璩�(zh矛)鐨勫垎闆㈢磾鍖栬垏绲�(ji茅)妲�(g貌u)鍒嗘瀽 閫忔瀽鍜�
瓒呮烤 铔嬬櫧璩�(zh矛)鐨勬矇娣€銆侀浕娉�銆佸堡鏋愬拰闆㈠績 铔嬬櫧璩�(zh矛)涓€绱氱祼(ji茅)妲�(g貌u)鍜岄珮绱氱祼(ji茅)妲�(g貌u)鐨勬脯瀹�
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｈ泲鐧借唱(zh矛)鐨勬蹇碉紝鎺屾彙铔嬬櫧璩�(zh矛)鐨勫垎瀛愮祫鎴愬拰姘ㄥ熀閰哥殑鐞嗗寲鎬ц唱(zh矛)锛岀啛鎮�
姘ㄥ熀閰哥殑鍒嗛銆�
锛�2锛夋帉鎻¤泲鐧借唱(zh矛)涓€绱氱祼(ji茅)妲�(g貌u)鍜岄珮绱氱祼(ji茅)妲�(g貌u)鐨勬蹇碉紝鐔熸倝缍郴鍚勭礆绲�(ji茅)妲�(g貌u)鐨勪富瑕佸寲瀛�(xu茅)
閸�銆�
锛�3锛夌悊瑙ｈ泲鐧借唱(zh矛)鐨勪竴绱氱祼(ji茅)妲�(g貌u)鑸囬珮绱氱祼(ji茅)妲�(g貌u)鍜屽姛鑳界殑闂�(gu膩n)绯讳互鍙婇珮绱氱祼(ji茅)妲�(g貌u)鑸囧姛鑳界殑
闂�(gu膩n)绯汇€�
锛�4锛夌啛鎮夎泲鐧借唱(zh矛)绲勭殑姒傚康锛岀悊瑙ｈ泲鐧借唱(zh矛)绲勫(xu茅)鐨勭爺绌舵妧琛�(sh霉)銆�
锛�5锛夋帉鎻¤泲鐧借唱(zh矛)绛夐浕榛炵殑姒傚康锛岀悊瑙ｈ泲鐧借唱(zh矛)鍏╂€цВ闆㈢殑鎬ц唱(zh矛)鍜岃啝楂旀€ц唱(zh矛)銆�
锛�6锛夋帉鎻¤泲鐧借唱(zh矛)璁婃€х殑瀹氱京銆佹湰璩�(zh矛)銆佺壒榛炲拰涓昏鐨勬噳(y墨ng)鐢ㄣ€�
锛�7锛夌悊瑙ｈ泲鐧借唱(zh矛)鐨勭传澶栧惛鏀剁壒鎬у拰铔嬬櫧璩�(zh矛)鐨勯洐绺劜鍙嶆噳(y墨ng)銆�.
锛�8锛夌悊瑙ｉ€忔瀽鍜岃秴婵剧殑姒傚康锛屾帉鎻¤泲鐧借唱(zh矛)娌夋穩鐨勬柟娉曞拰鍘熺悊銆�
锛�9锛夌悊瑙ｈ泲鐧借唱(zh矛)闆绘吵鐨勫師鐞嗗拰鍒嗛锛屼簡瑙ｅ叾涓昏鎳�(y墨ng)鐢�銆�
锛�10锛夌悊瑙ｈ泲鐧藉堡鏋愮殑鍘熺悊銆佸垎椤炲拰涓昏鎳�(y墨ng)鐢�銆�
锛�11锛変簡瑙ｈ泲鐧借唱(zh矛)搴忓垪鍒嗘瀽鍜岄珮绱氱祼(ji茅)妲�(g貌u)娓畾鐨勬柟娉曘€�
2銆佹牳閰哥殑绲�(ji茅)妲�(g貌u)鑸囧姛鑳�
鑰冭│鍏�(n猫i)瀹�
鏍搁吀鐨勭祫鎴愬強涓€绱氱祼(ji茅)妲�(g貌u) DNA 鐨勭┖闁撶祼(ji茅)妲�(g貌u)鑸囧姛鑳� RNA 鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳� 鏍�
閰哥殑鐞嗗寲鎬ц唱(zh矛) 鏍搁吀閰�銆�
鑰冭│瑕佹眰
锛�1锛変簡瑙ｆ牳閰哥殑绲勬垚鍜屽垎椤�锛屾帉鎻℃牳閰镐竴绱氱祼(ji茅)妲�(g貌u)鐨勬蹇�銆�
锛�2锛夋帉鎻� DNA 鐨勪簩绱氱祼(ji茅)妲�(g貌u)锛屼簡瑙� DNA 鐨勯珮绱氱祼(ji茅)妲�(g貌u)锛岀悊瑙� DNA 鐨勫姛鑳�锛�
锛�3锛夋帉鎻� RNA 鐨勫垎椤�銆佺祼(ji茅)妲�(g貌u)鐗归粸鍙婂悇绋� RNA 鐨勫姛鑳斤紝浜嗚В鏍搁吀鍦ㄥ師鏍哥窗(x矛)
鑳炲拰鐪熸牳绱�(x矛)鑳炰腑琛ㄧ従(xi脿n)鐨勪笉鍚屾檪绌虹壒鎬�銆�
锛�4锛夌悊瑙ｆ牳閰哥殑绱鍚告敹鐗规€э紝鎺屾彙 DNA 璁婃€х殑姒傚康銆佺壒榛�銆佸拰涓昏鎳�(y墨ng)
鐢�锛岀啛鎮夎畩鎬х殑涓昏鏂规硶锛岀悊瑙ｅ京(f霉)鎬у拰闆滀氦鐨勬蹇�銆�
锛�5锛夌悊瑙ｆ牳閰搁叾鐨勫垎椤炲拰浣滅敤鐗归粸銆�
3銆侀叾
鑰冭│鍏�(n猫i)瀹�
閰剁殑杓斿姪鍥犲瓙 閰剁殑娲绘€т腑蹇� 鍚屽伐閰� 閰朵績鍙嶆噳(y墨ng)鐨勭壒榛� 閰朵績鍙嶆噳(y墨ng)鐨勬鐞�
鍚勭ó鍥犵礌灏嶉叾淇冨弽鎳�(y墨ng)閫熷害鐨勫奖闊� 閰舵椿鎬х殑瑾�(di脿o)绡€(ji茅) 閰剁殑鍛藉悕鑸囧垎椤� 閰跺拰鐤剧梾
鐨勯棞(gu膩n)绯� 閰跺湪閱�(y墨)瀛�(xu茅)涓婄殑鎳�(y墨ng)鐢�
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｉ叾鐨勬蹇�锛屼簡瑙ｉ叾鐨勮紨鍔╁洜瀛�銆�
锛�2锛夋帉鎻￠叾鐨勬椿鎬т腑蹇冨拰鍚屽伐閰剁殑姒傚康锛岀悊瑙ｅ悓宸ラ叾鐨勮嚚搴婃噳(y墨ng)鐢�銆�
锛�3锛夌悊瑙ｉ叾鍙嶆噳(y墨ng)鐨勭壒榛炲拰閰朵績鍙嶆噳(y墨ng)鐨勬鍒躲€�
锛�4锛夌悊瑙ｅ簳鐗╂績搴﹁垏閰朵績鍙嶆噳(y墨ng)閫熷害鐨勯棞(gu膩n)绯诲拰绫虫皬鏂圭▼锛屾帉鎻� Km 鍊硷紝瀛�(xu茅)鏈�
浣滃湒娉曟眰鍙� Km 鍜� Vmax锛涗簡瑙ｉ叾婵冨害銆乸H 鍊�銆佹縺娲诲姂灏嶉叾淇冨弽鎳�(y墨ng)閫熷害鐨勫奖闊�銆�
锛�5锛夌悊瑙ｉ叾鐨勬姂鍒跺姂銆佸彲閫嗘姂鍒朵綔鐢ㄥ拰涓嶅彲閫嗘姂鍒朵綔鐢ㄧ殑姒傚康锛屼簡瑙ｆ湁姗�
纾蜂腑姣掑拰閲嶉噾灞腑姣掔殑姗熷埗銆�
锛�6锛夋帉鎻＄鐖€ф姂鍒朵綔鐢ㄧ殑姒傚康銆佸嫊鍔涘(xu茅)鐗归粸锛岀悊瑙ｇ：鑳洪钘ョ墿鐨勪綔鐢ㄦ
鍒�锛屼簡瑙ｉ潪绔剁埈鎬ф姂鍒朵綔鐢ㄥ拰鍙嶇鐖€ф姂鍒朵綔鐢ㄧ殑姒傚康鍜屽嫊鍔涘(xu茅)鐗归粸銆�
锛�7锛夌悊瑙ｉ叾鐨勮畩妲�(g貌u)瑾�(di脿o)绡€(ji茅)銆佸叡鍍逛慨椋捐(di脿o)绡€(ji茅)鍜岄叾鍘熺殑婵€娲�锛屼簡瑙ｉ叾鐨勮獦灏�(d菐o)鍜岄樆
閬忚(di脿o)绡€(ji茅)銆侀叾鐨勯檷瑙�锛岀啛鎮夐叾鐨勫懡鍚嶅拰鍒嗛銆�
锛�8锛夋帉鎻￠叾鑸囬啱(y墨)瀛�(xu茅)鐨勯棞(gu膩n)绯�锛岀啛鎮夐叾鍦ㄩ啱(y墨)瀛�(xu茅)涓婄殑鎳�(y墨ng)鐢�銆�
4銆佺硸浠ｈ瑵
鑰冭│鍏�(n猫i)瀹�
绯栫殑鐢熺悊鍔熻兘 绯栫殑娑堝寲鍚告敹 绯栫殑鐒℃哀閰佃В 绯栫殑鏈夋哀姘у寲 绯栦唬璎濈殑
纾烽吀鎴婄硸閫斿緫 绯栧師鐨勫悎鎴愯垏鍒嗚В 绯栫暟鐢� 琛€绯栧強鍏惰(di脿o)绡€(ji茅) 琛€绯栫暟甯歌垏绯栧翱
鐥�
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｇ硸鐨勫畾缇�銆佺硸鐨勭敓鐞嗗姛鑳�銆佺硸鐨勬秷鍖栧惛鏀�銆�
锛�2锛夋帉鎻＄硸閰佃В鐨勫弽鎳�(y墨ng)閬庣▼鍙婂叾闂�(gu膩n)閸甸叾锛岀悊瑙ｇ硸閰佃В鐨勭敓鐞嗘剰缇�銆�
锛�3锛夋帉鎻′笝閰吀鑴晶銆佷笁缇ч吀寰挵(hu谩n)鐨勫弽鎳�(y墨ng)閬庣▼鍙婂叾闂�(gu膩n)閸甸叾锛岀悊瑙ｄ笁缇ч吀寰�
鐠�(hu谩n)鐨勬剰缇╋紝鐞嗚В涓夌晶閰稿惊鐠�(hu谩n)涓� ATP 鐨勭敓鎴�銆�
锛�4锛夋帉鎻＄７閰告垔绯栭€斿緫鐨勫弽鎳�(y墨ng)閬庣▼闂�(gu膩n)閸甸叾鍙婂叾鐢熺悊鎰忕京銆�
锛�5锛夌悊瑙ｇ硸鍘熺殑鍚堟垚鍜屽垎瑙ｇ殑鍙嶆噳(y墨ng)姝ラ锛屾帉鎻＄硸鍘熷悎鎴愬拰鍒嗚В鐨勯棞(gu膩n)閸甸叾锛�
浜嗚В绯栧師绱⿳鐧�銆�
锛�6锛夋帉鎻＄硸鐣扮敓鐨勬蹇�锛涚悊瑙ｇ硸鐣扮敓鐨勫弽鎳�(y墨ng)閬庣▼锛涗簡瑙ｇ硸鐣扮敓鐨勭敓鐞嗘剰缇�锛�
鐞嗚В涔抽吀寰挵(hu谩n)鍙婂叾鐢熺悊鎰忕京銆�
锛�7锛夌悊瑙ｈ绯栫殑姒傚康锛屾帉鎻¤绯栦締婧愯垏鍘昏矾锛岀悊瑙ｈ(di脿o)绡€(ji茅)琛€绯栫殑婵€绱犲強鍏朵綔
鐢ㄦ鍒�锛涗簡瑙ｈ绯栧崌楂樺拰闄嶄綆鎵€鑷寸殑鐤剧梾銆�
5銆佽剛椤炰唬璎�
鑰冭│鍏�(n猫i)瀹�
鑴傞吀鐨勫懡鍚嶈垏鍒嗛 鑴傞鐨勬秷鍖栧惛鏀� 鐢樻补涓夐叝浠ｈ瑵 纾疯剛浠ｈ瑵 鑶藉浐閱�
浠ｈ瑵 琛€婕胯剛铔嬬櫧浠ｈ瑵
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｈ剛椤炵殑瀹氱京銆佷笉椋藉拰鑴傝偑閰哥殑鍛藉悕鑸囧垎椤�锛屾帉鎻＄嚐椁�(y菐ng)蹇呴渶鑴傝偑閰�
鐨勫畾缇╁拰绋銆�.
锛�2锛変簡瑙ｈ剛椤炵殑娑堝寲鍜岃剛椤炵殑鍚告敹銆�
锛�3锛変簡鑴傞鐨勪富瑕佸劜瀛樺舰寮�锛岀悊瑙ｇ敇娌逛笁閰殑鐢熺悊鍔熻兘锛屾帉鎻¤剛鑲嫊鍝＄殑
姒傚康鍙婂叾闂�(gu膩n)閸甸叾锛涚悊瑙ｈ剛瑙ｆ縺绱犲拰鎶楄剛瑙ｆ縺绱狅紱鐞嗚В鐢樻补鐨勫垎瑙ｄ唬璎�锛屾帉鎻¤剛閰�
鐨勎� -姘у寲鍒嗚В锛屾帉鎻￠叜楂旂殑鐢熸垚鑸囧埄鐢ㄤ互鍙婇叜楂旂敓鎴愮殑鐢熺悊鎰忕京锛岀悊瑙ｈ剛閰哥殑
鍚堟垚鍘熸枡鍜岄棞(gu膩n)閸甸叾锛岀啛鎮夎剛閰哥⒊閺堢殑寤堕暦锛岀悊瑙ｈ剛閰稿悎鎴愮殑瑾�(di脿o)绡€(ji茅)锛岀啛鎮夌敇娌逛笁閰�
鐨勫悎鎴愶紝浜嗚В鍓嶅垪鑵虹礌銆佽鏍撶兎銆佺櫧涓夌儻鐨勪富瑕佸姛鑳姐€�
锛�4锛夌悊瑙ｇ７鑴傜殑绲勬垚鍜岀７鑴傜殑鍔熻兘锛屼簡瑙ｇ敇娌圭７鑴傜殑鍚堟垚鑸囬檷瑙ｃ€�
锛�5锛夋帉鎻¤喗鍥洪唶鍚堟垚鐨勯儴浣�銆佸師鏂欏拰鍩烘湰鐨勫弽鎳�(y墨ng)閬庣▼锛屼簡瑙ｈ喗鍥洪唶鍚堟垚鐨�
瑾�(di脿o)绡€(ji茅)銆�
锛�6锛夌悊瑙ｈ鑴傜殑姒傚康锛屼簡瑙ｈ婕胯剛铔嬬櫧鐨勫垎椤�锛屾帉鎻¤婕胯剛铔嬬櫧鐨勫姛鑳斤紝
浜嗚В琛€婕胯剛铔嬬櫧浠ｈ瑵鐣板父鑸囬伜鍌冲拰鐤剧梾鐨勯棞(gu膩n)绯�銆�
6銆佺敓鐗╂哀鍖�
鑰冭│鍏�(n猫i)瀹�
姘у寲鍛煎惛閺� 姘у寲纾烽吀鍖� 绶氱矑楂斿収(n猫i)鑶滃皪鐗╄唱(zh矛)鐨勯伕鎿囨€ц綁(zhu菐n)閬� 涓嶇敓鎴� ATP
鐨勬哀鍖栭珨绯�
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｇ敓鐗╂哀鍖栥€侀浕瀛愬偝閬為張鐨勬蹇�锛屾帉鎻￠浕瀛愬偝閬為張鐨勭祫鎴愬拰鎺掑垪闋�
搴�銆�
锛�2锛夋帉鎻℃哀鍖栫７閰稿寲鐨勬蹇�锛屼簡瑙ｆ哀鍖栫７閰稿寲鍋惰伅(li谩n)鐨勬鍒�锛岀悊瑙ｅ奖闊挎哀鍖�
纾烽吀鍖栫殑鍥犵礌锛屼簡瑙� ATP 鍦ㄨ兘閲忕敓鎴�銆佸埄鐢�銆佸劜瀛樺拰杞�(zhu菐n)绉讳腑鐨勪綔鐢ㄣ€�.
锛�3锛夌悊瑙ｇ窔绮掗珨鍏�(n猫i)鑶滃皪鍚勭ó鐗╄唱(zh矛)鐨勯伕鎿囨€ц綁(zhu菐n)绉�銆�
锛�4锛変簡瑙ｆ姉姘у寲閰堕珨绯诲拰寰矑楂旂窗(x矛)鑳炶壊绱� P450 鍠姞姘ч叾鐨勫姛鑳�銆�
7銆佹皑鍩洪吀浠ｈ瑵
鑰冭│鍏�(n猫i)瀹�
铔嬬櫧璩�(zh矛)鐨勭嚐椁�(y菐ng)浣滅敤 姘钩琛″拰铔嬬櫧璩�(zh矛)鐨勭嚐椁�(y菐ng)鍍瑰€� 铔嬬櫧璩�(zh矛)鐨勬秷鍖�銆佸惛鏀跺拰鑵�
鏁� 姘ㄥ熀閰哥殑鑴皑鍩轰綔鐢� 姘ㄥ熀閰哥⒊閺堥鏋剁殑杞�(zhu菐n)鎻涜垏鍒嗚В 楂斿収(n猫i)姘ㄧ殑渚嗘簮鑸囧幓璺�
灏跨礌鍚堟垚 鍊嬪垾姘ㄥ熀閰哥殑浠ｈ瑵 姘ㄥ熀閰哥殑鑴晶鍩轰綔鐢� 涓€纰冲柈浣嶇殑鐢熸垚鑸囧姛鑳�
鍚～姘ㄥ熀閰哥殑浠ｈ瑵 鑺抽鏃忔皑鍩洪吀浠ｈ瑵 鏀張姘ㄥ熀閰哥殑鍒嗚В
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｈ泲鐧借唱(zh矛)鐨勫姛鑳�锛屾帉鎻℃爱骞宠 銆佺嚐椁�(y菐ng)蹇呴渶姘ㄥ熀閰�銆佽泲鐧借唱(zh矛)鐕熼(y菐ng)鍍瑰€�
鐨勬蹇�銆�
锛�2锛夌悊瑙ｈ泲鐧借唱(zh矛)娑堝寲鐨勫牬鎵€鍜岄亷绋�銆佸惛鏀剁殑褰㈠紡鍜屼富瑕侀儴浣�锛屼簡瑙ｈ泲鐧借唱(zh矛)
鐨勮厫鏁椾綔鐢�銆�
锛�3锛夋帉鎻℃皑鍩洪吀鐨勮劔姘ㄥ熀浣滅敤锛岀悊瑙ｅ槍鍛ゆ牳鑻烽吀寰挵(hu谩n)锛屼簡瑙ｆ皑鍩洪吀纰抽張楠�
鏋剁殑浠ｈ瑵銆�
锛�4锛夋帉鎻￠珨鍏�(n猫i)姘ㄧ殑渚嗘簮鍜屾皑鐨勫幓璺�锛屾帉鎻′笝姘ㄩ吀-钁¤悇绯栧惊鐠�(hu谩n)鍙婂叾鎰忕京锛屼簡
瑙ｉ珨鍏�(n猫i)姘ㄧ殑杞�(zhu菐n)閬�锛屾帉鎻″翱绱犲悎鎴愮殑槌ユ皑閰稿惊鐠�(hu谩n)瀛�(xu茅)瑾�锛岀悊瑙ｅ翱绱犲悎鎴愮殑瑾�(di脿o)绡€(ji茅)銆�
锛�5锛夌悊瑙ｆ皑鍩洪吀鐨勮劔缇у熀浣滅敤锛屾帉鎻′竴纰冲柈浣嶇殑瀹氱京锛岀悊瑙ｄ竴纰冲柈浣嶇敓鎴�
鍙婂叾鍔熻兘銆�
锛�6锛夋帉鎻＄敳纭皑閰稿惊鐠�(hu谩n)锛岀悊瑙ｈ姵棣欐棌姘ㄥ熀閰哥殑浠ｈ瑵锛屼簡瑙ｆ敮閺堟皑鍩洪吀鐨勪唬
璎�銆�
8銆佹牳鑻烽吀浠ｈ瑵
鑰冭│鍏�(n猫i)瀹�
鏍搁吀鐨勬秷鍖栧拰鏍歌嫹閰哥殑鐢熺墿瀛�(xu茅)鍔熺敤 鍢屽懁鏍歌嫹閰哥殑鍚堟垚鑸囧垎瑙� 鍢屽懁鏍�
鑻烽吀鐨勭浉浜掕綁(zhu菐n)璁� 鑴哀鏍歌嫹閰哥殑鐢熸垚 鍢屽懁鏍歌嫹閰哥殑鎶椾唬璎濈墿 鍢у暥鏍歌嫹閰哥殑鍚�
鎴愯垏鍒嗚В 鍢у暥鏍歌嫹閰哥殑鎶椾唬璎濈墿
鑰冭│瑕佹眰
锛�1锛変簡瑙ｆ牳閰哥殑娑堝寲鍜屾牳鑻烽吀鐨勭敓鐗╁(xu茅)鍔熺敤銆�
锛�2锛夋帉鎻″槍鍛ゆ牳鑻烽吀鐨勫緸闋悎鎴愰€斿緫锛岀悊瑙ｅ槍鍛ゆ牳鑻烽吀鐨勮鏁戝悎鎴愰€斿緫锛�
浜嗚В鍢屽懁鏍歌嫹閰哥殑鐩镐簰杞�(zhu菐n)璁婏紝鐞嗚В鑴哀鏍歌嫹閰哥殑鐢熸垚銆�
锛�3锛夌悊瑙ｅ槍鍛ゆ牳鑻烽吀鐨勬姉浠ｈ瑵鐗╁強鍏舵噳(y墨ng)鐢�锛屾帉鎻″槍鍛ゆ牳鑻烽吀鐨勫垎瑙ｄ唬璎�銆�
锛�4锛夋帉鎻″槯鍟舵牳鑻烽吀鐨勫緸闋悎鎴愰€斿緫锛岀悊瑙ｅ槯鍟舵牳鑻烽吀鐨勮鏁戝悎鎴愰€斿緫銆�
锛�5锛夌悊瑙ｅ槯鍟舵牳鑻烽吀鐨勬姉浠ｈ瑵鐗╁強鍏舵噳(y墨ng)鐢紟
锛�6锛変簡瑙ｅ槯鍟舵牳鑻烽吀鐨勫垎瑙ｄ唬璎�銆�
9銆佺墿璩�(zh矛)浠ｈ瑵鐨勮伅(li谩n)绯昏垏瑾�(di脿o)绡€(ji茅)
鑰冭│鍏�(n猫i)瀹�
顥ヮ棩鐗╄唱(zh矛)浠ｈ瑵鐨勭壒榛� 鐗╄唱(zh矛)浠ｈ瑵鐨勭浉浜掕伅(li谩n)绯� 楂斿収(n猫i)閲嶈绲勭箶銆佸櫒瀹樼殑浠ｈ瑵鐗归粸鍙�
鑱�(li谩n)绯� 浠ｈ瑵瑾�(di脿o)绡€(ji茅)鐨勬柟寮� 浠ｈ瑵绲勫(xu茅)
鑰冭│瑕佹眰
锛�1锛変簡瑙ｄ唬璎濊(di脿o)绡€(ji茅)鐨勭壒榛�銆�
锛�2锛夌悊瑙ｅ悇绋兘婧愮墿璩�(zh矛)鐨勪唬璎濊伅(li谩n)绯昏垏鍒剁磩锛屾帉鎻＄硸銆佽剛銆佽泲鐧借唱(zh矛)鐨勭浉浜掕伅(li谩n)
绯�銆�
锛�3锛夌悊瑙ｈ倽鍦ㄧ墿璩�(zh矛)浠ｈ瑵涓殑浣滅敤锛屼簡瑙ｅ績銆佽叇銆佽倢鑲夈€佺磪绱�(x矛)鑳炲皪鑳芥簮鐗╄唱(zh矛)
鐨勫埄鐢�锛岀啛鎮夎剛鑲祫绻斿拰鑵庣殑鍔熻兘銆�
锛�4锛夌悊瑙ｇ窗(x矛)鑳炴按骞崇殑浠ｈ瑵瑾�(di脿o)绡€(ji茅)锛屼簡瑙ｆ縺绱犲皪浠ｈ瑵閬庣▼鐨勮(di脿o)绡€(ji茅)锛岀啛鎮夌墿璩�(zh矛)浠�
璎濈殑绁炵稉(j墨ng)-楂旀恫瑾�(di脿o)绡€(ji茅)銆�
锛�5锛夌悊瑙ｄ唬璎濈祫瀛�(xu茅)鐨勬蹇碉紝浜嗚В浠ｈ瑵绲勫(xu茅)鐨勬噳(y墨ng)鐢�銆�
10銆丏NA 鐢熺墿鍚堟垚
鑰冭│鍏�(n猫i)瀹�
顥ヮ棩顥ヤ腑蹇冩硶鍓� 寰�(f霉)鍒剁殑姒傚康 寰�(f霉)鍒剁殑鍩烘湰瑕�(gu墨)寰� DNA 寰�(f霉)鍒剁殑閰跺(xu茅)鍩虹(ch菙)鍜屾嫇
鎾插(xu茅)璁婂寲 DNA 鐢熺墿鍚堟垚鐨勯亷绋� 閫嗚綁(zhu菐n)閷� D 鐠�(hu谩n)寰�(f霉)鍒� DNA 鎼嶅偡鑸囦慨寰�(f霉)
鑰冭│瑕佹眰
顥ワ紙1锛夌悊瑙ｄ腑蹇冩硶鍓囩殑姒傚康銆�
锛�2锛夌悊瑙ｅ京(f霉)鍒剁殑姒傚康锛屾帉鎻″京(f霉)鍒剁殑鍩烘湰瑕�(gu墨)寰�锛屾帉鎻″崐淇濈暀寰�(f霉)鍒�銆侀洐鍚戝京(f霉)鍒�銆�
鍗婁笉閫ｇ簩(x霉)寰�(f霉)鍒剁殑姒傚康锛屼簡瑙ｅ崐淇濈暀 DNA 寰�(f霉)鍒剁殑瀵﹂渚濇摎(j霉)銆�
锛�3锛夌悊瑙� DNA 寰�(f霉)鍒堕渶瑕佺殑鐢熺墿鍒嗗瓙鍜屽京(f霉)鍒剁殑鍩烘湰鍖栧(xu茅)鍙嶆噳(y墨ng)锛屾帉鎻″師鏍哥敓鐗�
鐨� DNA 鑱氬悎閰跺強鍏舵椿鎬х壒榛�锛屾帉鎻＄湡鏍哥敓鐗╃殑 DNA 鑱氬悎閰跺強鍏舵椿鎬х壒榛�锛岀悊
瑙ｅ京(f霉)鍒朵繚鐪熸€х殑閰跺(xu茅)渚濇摎(j霉)锛屼簡瑙ｅ京(f霉)鍒朵腑 DNA 鍒嗗瓙鐨勬嫇?f霉)閶礧(xu茅)璁婂寲锛岀悊瑙� DNA 閫�
鎺ラ叾鍙婂叾浣滅敤鐗归粸銆�
锛�4锛夋帉鎻″師鏍哥敓鐗� DNA 鐢熺墿鍚堟垚鐨勯亷绋�锛岀悊瑙ｇ湡鏍哥敓鐗╃殑 DNA 鐢熺墿鍚堟垚锛�
鎺屾彙绔矑鐨勬蹇靛強鍏剁敓鐗╁(xu茅)鍔熻兘锛岀悊瑙ｄ汉椤炵殑绔矑閰跺強鍏舵椿鎬х壒榛�銆�
锛�5锛夋帉鎻￠€嗚綁(zhu菐n)閷勭殑姒傚康锛岀悊瑙ｉ€嗚綁(zhu菐n)閷勭殑閬庣▼锛屼簡瑙ｇ櫦(f膩)鐝�(xi脿n)閫嗚綁(zhu菐n)閷勭殑鐢熺墿瀛�(xu茅)鎰忕京銆�
鐔熸倝 D 鐠�(hu谩n)寰�(f霉)鍒�銆�
锛�6锛夌悊瑙ｇ獊璁婄殑鐢熺墿瀛�(xu茅)鎰忕京锛屼簡瑙ｅ紩璧风獊璁婄殑鐗╃悊銆佸寲瀛�(xu茅)鍥犵礌锛屾帉鎻＄獊璁婄殑
椤炲瀷锛岀悊瑙� DNA 鎼嶅偡淇京(f霉)鐨勪富瑕侀鍨�銆�
11銆丷NA 鐨勭敓鐗╁悎鎴�
鑰冭│鍏�(n猫i)瀹�
杞�(zhu菐n)閷勭殑姒傚康 寰�(f霉)鍒惰垏杞�(zhu菐n)閷勭殑鐩稿悓榛炲拰涓嶅悓榛� 杞�(zhu菐n)閷勭殑妯℃澘鍜岄叾 鍘熸牳鐢熺墿
鐨勮綁(zhu菐n)閷勯亷绋� 鐪熸牳鐢熺墿 RNA 鐨勭敓鐗╁悎鎴� 鐪熸牳鐢熺墿 RNA 鐨勫姞宸�
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｈ綁(zhu菐n)閷勭殑姒傚康锛屾帉鎻″京(f霉)鍒跺拰杞�(zhu菐n)閷勭殑鐩稿悓榛炲拰涓嶅悓榛炪€�
锛�2锛夌悊瑙ｇ祼(ji茅)妲�(g貌u)鍩哄洜銆佷笉灏嶇ū杞�(zhu菐n)闄哥殑姒傚康锛屾帉鎻″師鏍哥敓鐗� RNA 鑱氬悎閰剁殑绲�
鎴�銆佸悇浜炲熀鐨勫姛鑳藉拰閰剁殑娲绘€х壒榛�锛岀悊瑙� RNA 鑱氬悎閰惰垏 DNA 鍟熷嫊瀛愮殑绲�(ji茅)鍚堝強
RNA 鑱氬悎閰跺偓鍖栫殑 RNA 鐢熺墿鍚堟垚銆�
锛�3锛夋帉鎻″師鏍哥敓鐗╃殑杞�(zhu菐n)閷勯亷绋嬶紝浜嗚В杞�(zhu菐n)閷勫欢闀峰拰缈昏鍚屾檪閫�(j矛n)琛岀殑鐝�(xi脿n)璞�锛岀悊
瑙ｄ緷璩聪� 鍥犲瓙鐨勮綁(zhu菐n)閷勭祩姝㈠拰涓嶄緷璩聪� 鍥犲瓙鐨勮綁(zhu菐n)閷勭祩姝€€�
锛�4锛夌悊瑙ｇ湡鏍哥敓鐗╃殑 RNA 鑱氬悎閰剁殑绋鍜屽姛鑳�锛屾帉鎻￠爢寮忎綔鐢ㄥ厓浠躲€佸弽
寮忎綔鐢ㄥ洜瀛�銆佽綁(zhu菐n)閷勫洜瀛愮殑鐨勬蹇�锛岀悊瑙ｇ湡鏍哥敓鐗╃殑杞�(zhu菐n)閷勮捣濮嬮亷绋嬨€佸悇绋綁(zhu菐n)閷勫洜瀛�
鐨勪綔鐢�锛屼簡瑙ｆ嫾鏉跨悊璜栧拰杞�(zhu菐n)閷勫欢闀风殑鐗归粸锛岀啛鎮夌湡鏍哥敓鐗╄綁(zhu菐n)閷勭祩姝㈢殑閬庣▼銆�
锛�5锛夋帉鎻＄湡鏍哥敓鐗� mRNA 鐨勮綁(zhu菐n)閷勫姞宸ラ亷绋�锛岀悊瑙ｆ柗瑁傚熀鍥�銆佸椤瓙銆佸収(n猫i)鍚�
瀛愮殑姒傚康锛岀悊瑙� mRNA 鐨勫壀鎺ャ€�
锛�6锛変簡瑙� rRNA銆乼RNA 鐨勫姞宸ラ亷绋嬪拰 RNA 鍌寲鐨勮嚜鎴戝壀鎺ャ€�
12銆佽泲鐧借唱(zh矛)鐨勭敓鐗╁悎鎴�
鑰冭│鍏�(n猫i)瀹�:
缈昏 铔嬬櫧璩�(zh矛)鐢熺墿鍚堟垚楂旂郴 閬哄偝瀵嗙⒓鐨勭壒榛� 姘ㄥ熀閰哥殑娲诲寲 鍘熸牳鐢熺墿
鐨勮偨閺堝悎鎴愰亷绋� 鐪熸牳鐢熺墿鐨勮偨閺堝悎鎴愰亷绋� 铔嬬櫧璩�(zh矛)缈昏鍚庣殑淇＞ 铔嬬櫧璩�(zh矛)鐨勯澏
鍚戣几閫� 铔嬬櫧璩�(zh矛)鐢熺墿鍚堟垚鐨勫共鎿惧拰鎶戝埗
鑰冭│瑕佹眰
锛�1锛夋帉鎻＄炕璀殑姒傚康锛�
锛�2锛夌悊瑙ｄ笁绋� RNA 鍦ㄨ泲鐧借唱(zh矛)鐢熺墿鍚堟垚涓殑浣滅敤銆�
锛�3锛夌悊瑙ｉ枊鏀鹃柋璁€妗嗘灦鐨勬蹇�锛屾帉鎻￠伜鍌冲瘑纰肩殑鐗归粸锛屼簡瑙ｈ泲鐧借唱(zh矛)鐢熺墿鍚�
鎴愰渶瑕佺殑閰堕鍜岃泲鐧借唱(zh矛)鍥犲瓙銆�
锛�4锛夋帉鎻℃皑鍩洪吀娲诲寲鐨勯亷绋嬪強姘ㄥ熀閰�-tRNA 鍚堟垚閰跺湪姘ㄥ熀閰歌垏 tRNA 姝ｇ⒑
绲�(ji茅)鍚堜腑鐨勪綔鐢�锛岀悊瑙ｈ捣濮嬫皑鍩洪叞-tRNA 鍚堟垚銆�
锛�5锛夋帉鎻″師鏍哥敓鐗╃殑鑲介張鍚堟垚閬庣▼鍙婂悇绋泲鐧借唱(zh矛)鍥犲瓙鐨勪綔鐢�锛屽(xu茅)鏈冭▓绠楄泲
鐧借唱(zh矛)鍚堟垚涓兘閲忕殑娑堣€楋紝鐞嗚В鐪熸牳鐢熺墿鐨勮偨閺堝悎鎴愰亷绋�锛屾瘮杓冨畠鍊戠殑涓昏宸垾銆�
锛�6锛夋帉鎻″垎瀛愪即渚剁殑姒傚康锛屼簡瑙ｅ鑲介張鎶樼枈褰㈡垚澶╃劧妲�(g貌u)鍍忕殑铔嬬櫧璩�(zh矛)锛岀悊瑙�
鑲介張鐨勬按瑙ｅ拰鍖栧(xu茅)淇＞锛屼簡瑙ｄ簽鍩虹殑鑱氬悎鍜岃紨鍩虹殑閫ｆ帴銆�
锛�7锛変簡瑙ｈ泲鐧借唱(zh矛)鐨勯澏鍚戣几閫�锛岀悊瑙ｄ俊铏熷簭鍒椼€佷俊铏熻偨銆佹牳瀹氫綅搴忓垪鐨勬蹇�銆�
锛�8锛夌悊瑙ｆ姉鐢熺礌鐨勬蹇�锛屾帉鎻′富瑕佹姉鐢熺礌鎶戝埗铔嬬櫧璩�(zh矛)鐢熺墿鍚堟垚鐨勫師鐞�锛屼簡
瑙ｆ瘨绱犲拰骞叉摼绱犵殑浣滅敤銆�
13銆佸熀鍥犺〃閬�(d谩)瑾�(di脿o)鎺�
鑰冭│鍏�(n猫i)瀹�:
鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺х殑鍩烘湰姒傚康 鍩哄洜琛ㄩ仈(d谩)鐨勬檪闁撳拰绌洪枔鐗圭暟鎬� 鍩哄洜琛ㄩ仈(d谩)鐨勬柟
寮� 鍩哄洜琛ㄩ仈(d谩)鐨勭敓鐗╁(xu茅)鎰忕京 鍩哄洜琛ㄩ仈(d谩)鐨勫熀鏈師鐞� 鍘熸牳鐢熺墿鍩哄洜杞�(zhu菐n)閷勮(di脿o)绡€(ji茅)鐗归粸
鍘熸牳鐢熺墿鍩哄洜杞�(zhu菐n)閷勮(di脿o)绡€(ji茅)鐨勬搷绺卞瓙姗熷埗 鍘熸牳鐢熺墿杞�(zhu菐n)閷勭祩姝㈢殑瑾�(di脿o)绡€(ji茅)姗熷埗 鍘熸牳鐢�
鐗╃炕璀按骞崇殑瑾�(di脿o)绡€(ji茅) 鐪熸牳鐢熺墿鍩哄洜绲勭殑绲�(ji茅)妲�(g貌u)鐗归粸 鐪熸牳鐢熺墿鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺х殑鐗归粸
RNA 鑱氬悎閰垛叀?q奴)顬扗(zhu菐n)閷勮捣濮嬬殑瑾�(di脿o)绡€(ji茅) 鐪熸牳鐢熺墿杞�(zhu菐n)閷勫悗姘村钩鐨勮(di脿o)绡€(ji茅) 鐪熸牳鐢熺墿缈昏
姘村钩鐨勮(di脿o)绡€(ji茅) 灏忓垎瀛� RNA 灏嶅熀鍥犺〃閬�(d谩)鐨勮(di脿o)绡€(ji茅)
鑰冭│瑕佹眰:
锛�1锛夌悊瑙ｅ熀鍥犵祫銆佸熀鍥犺〃閬�(d谩)鐨勬蹇�锛屾帉鎻″熀鍥犺〃閬�(d谩)鐨勬檪闁撳拰绌洪枔鐗圭暟鎬�锛�
鐞嗚В鍩烘湰琛ㄩ仈(d谩)銆佽獦灏�(d菐o)鍜岄樆閬忚〃閬�(d谩)锛屾帉鎻″熀鍥犺〃閬�(d谩)鐨勭敓鐗╁(xu茅)鎰忕京銆�
锛�2锛夌悊瑙ｅ熀鍥犺〃閬�(d谩)瑾�(di脿o)鎺х殑鍩烘湰鍘熺悊锛屾帉鎻″熀鍥犺綁(zhu菐n)閷勬縺娲荤殑鍩烘湰瑕佺礌銆�
锛�3锛夌悊瑙ｅ師鏍哥敓鐗╁熀鍥犺綁(zhu菐n)閷勮(di脿o)绡€(ji茅)鐨勭壒榛�锛屾帉鎻″師鏍哥敓鐗╁熀鍥犺綁(zhu菐n)閷勮捣濮嬭(di脿o)绡€(ji茅)
鐨勬搷绺卞瓙姗熷埗锛岀悊瑙ｅ師鏍哥敓鐗╀笉鍚岀殑杞�(zhu菐n)閷勭祩姝㈣(di脿o)绡€(ji茅)姗熷埗锛屼簡瑙ｅ師鏍哥敓鐗╃炕璀按骞�
鐨勮(di脿o)绡€(ji茅)銆�
锛�4锛夌悊瑙ｇ湡鏍哥敓鐗╁熀鍥犵祫鐨勭祼(ji茅)妲�(g貌u)鐗归粸鍜屽熀鍥犺〃閬�(d谩)瑾�(di脿o)鎺х殑鐗归粸锛屼簡瑙� RNA
鑱氬悎閰垛厾鍜� RNA 鑱氬悎閰垛參杞�(zhu菐n)閷勯珨绯荤殑瑾�(di脿o)绡€(ji茅)锛屾帉鎻� RNA 鑱氬悎閰垛叀鍙冭垏鐨勮綁(zhu菐n)閷勮捣濮�
鐨勮(di脿o)绡€(ji茅)锛屾帉鎻″暉鍕曞瓙銆佸寮峰瓙銆佹矇榛樺瓙鐨勬蹇�锛屼簡瑙ｈ綁(zhu菐n)閷勮(di脿o)绡€(ji茅)鍥犲瓙鐨勭祼(ji茅)妲�(g貌u)銆�
锛�5锛変簡瑙ｇ湡鏍哥敓鐗╄綁(zhu菐n)閷勫悗姘村钩鐨勮(di脿o)绡€(ji茅)鍜岀炕璀按骞崇殑瑾�(di脿o)绡€(ji茅)銆�
锛�7锛夌悊瑙ｅ皬鍒嗗瓙 RNA 灏嶅熀鍥犺〃閬�(d谩)鐨勮(di脿o)绡€(ji茅)锛岀悊瑙ｅ井灏� RNA 鍜屽皬骞叉摼 RNA 鐨勬
蹇�銆�
14銆佸熀鍥犻噸绲勮垏鍩哄洜宸ョ▼
鑰冭│鍏�(n猫i)瀹�
鍚屾簮閲嶇祫 閲嶇祫 DNA 鎶€琛�(sh霉)鐨勭浉闂�(gu膩n)姒傚康 閲嶇祫 DNA 鎶€琛�(sh霉)鐨勫熀鏈師鐞� 閲嶇祫
DNA 鎶€琛�(sh霉)鑸囬啱(y墨)瀛�(xu茅)鐨勯棞(gu膩n)绯�
鑰冭│瑕佹眰
锛�1锛夋帉鎻″悓婧愰噸绲勭殑姒傚康锛屼簡瑙ｄ綅榛炵壒鐣版€ч噸绲勫拰杞�(zhu菐n)搴ч噸绲勩€�
锛�2锛夋帉鎻￠噸绲� DNA 鎶€琛�(sh霉)鐨勫熀鏈蹇�锛岀悊瑙ｉ噸绲� DNA 鎶€琛�(sh霉)鐨勫伐鍏烽叾鍜岃級楂旓紝
鎺屾彙閲嶇祫 DNA 鎶€琛�(sh霉)鐨勫熀鏈師鐞嗗拰鎿嶄綔姝ラ锛岀悊瑙ｇ嵅鍙栫洰鐨勫熀鍥犵殑鏂规硶锛岀悊瑙ｉ噸
绲� DNA 灏�(d菐o)鍏ュ涓荤窗(x矛)鑳炵殑鏂规硶锛屼簡瑙ｉ噸绲勬彁绡╅伕鐨勬柟娉曘€�
锛�3锛夌悊瑙ｉ噸绲� DNA 鎶€琛�(sh霉)鍦ㄩ啱(y墨)瀛�(xu茅)涓婄殑鎳�(y墨ng)鐢�銆�
15銆佺窗(x矛)鑳炰俊铏熻綁(zhu菐n)灏�(d菐o)
鑰冭│鍏�(n猫i)瀹�
淇¤櫉杞�(zhu菐n)灏�(d菐o)鐨勫畾缇� 绱�(x矛)鑳炰俊铏熻綁(zhu菐n)灏�(d菐o)鐨勫熀鏈矾绶� 绱�(x矛)鑳炲鍖栧(xu茅)淇¤櫉 鍙楅珨鐨勫畾缇�銆�
鐗归粸鍜屽垎甯� 绱�(x矛)鑳炶綁(zhu菐n)灏�(d菐o)淇¤櫉鐨勬柟寮� 绱�(x矛)鑳炲収(n猫i)淇¤櫉杞�(zhu菐n)灏�(d菐o)鐩搁棞(gu膩n)鐨勫垎瀛� 鍩烘湰淇¤櫉杞�(zhu菐n)灏�(d菐o)閫�
璺� 淇¤櫉杞�(zhu菐n)灏�(d菐o)閬庣▼鐨勮(gu墨)寰嬭垏鐗归粸 绱�(x矛)鑳炰俊铏熻綁(zhu菐n)灏�(d菐o)鑸囬啱(y墨)瀛�(xu茅)
鑰冭│瑕佹眰
锛�1锛夋帉鎻′俊铏熻綁(zhu菐n)灏�(d菐o)鐨勬蹇�锛岀悊瑙ｇ窗(x矛)鑳炰俊铏熻綁(zhu菐n)灏�(d菐o)鐨勫熀鏈矾绶氬拰鏂瑰紡锛屼簡瑙ｇ窗(x矛)
鑳炲鍖栧(xu茅)淇¤櫉銆�
锛�2锛夋帉鎻″彈楂旂殑姒傚康鍜岀壒榛�锛岀悊瑙ｅ彈楂旂殑鍒嗗竷锛屼簡瑙ｄ俊铏熻綁(zhu菐n)灏�(d菐o)閫氳矾鍙婄窗(x矛)鑳�
杞�(zhu菐n)鍒颁俊铏熺殑鍩烘湰鏂瑰紡銆�
锛�3锛夋帉鎻＄窗(x矛)鑳炲収(n猫i)閲嶈鐨勪簩绱氫俊浣�锛岀悊瑙ｄ簩绱氫俊浣跨殑鐢熸垚锛屼簡瑙ｄ簩绱氫俊浣垮弮
鑸囪(di脿o)绡€(ji茅)鐨勯澏鍒嗗瓙銆�
锛�4锛夌悊瑙ｇ窗(x矛)鑳炲収(n猫i)鐨勪俊铏熻綁(zhu菐n)灏�(d菐o)鍒嗗瓙銆�
锛�5锛夋帉鎻＄窗(x矛)鑳炲収(n猫i)鍙楅珨浠嬪皫(d菐o)鐨勪俊铏熻綁(zhu菐n)灏�(d菐o)閫斿緫锛岀悊瑙ｉ洟瀛愰€氶亾鍨嬪彈楂斾粙灏�(d菐o)鐨勪俊
铏熻綁(zhu菐n)灏�(d菐o)閫斿緫锛屾帉鎻′竷璺ㄨ啘鍙楅珨浠嬪皫(d菐o)鐨勪俊铏熻綁(zhu菐n)灏�(d菐o)閫斿緫锛岀悊瑙ｅ柈璺ㄨ啘鍙楅珨浠嬪皫(d菐o)鐨勪俊铏�
杞�(zhu菐n)灏�(d菐o)閫斿緫锛屼簡瑙ｄ俊铏熻綁(zhu菐n)灏�(d菐o)閬庣▼鐨勭壒榛炶垏瑕�(gu墨)寰�銆�
锛�6锛変簡瑙ｄ俊铏熻綁(zhu菐n)灏�(d菐o)鑸囬啱(y墨)瀛�(xu茅)鐨勯棞(gu膩n)绯�銆�
16銆佽娑茬殑鐢熺墿鍖栧(xu茅)
鑰冭│鍏�(n猫i)瀹�
琛€婕胯泲鐧界殑鍒嗛鑸囨€ц唱(zh矛) 琛€婕胯泲鐧借唱(zh矛)鐨勫姛鑳� 绱呯窗(x矛)鑳炵殑浠ｈ瑵鐗归粸 鐧界窗(x矛)鑳�
鐨勪唬璎�
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｈ婕胯泲鐧借唱(zh矛)鐨勫垎椤炲拰鎬ц唱(zh矛)锛屾帉鎻¤婕胯泲鐧借唱(zh矛)鐨勫姛鑳姐€�
锛�2锛夌悊瑙ｇ磪绱�(x矛)鑳炵硸浠ｈ瑵鐨勪富瑕侀€斿緫锛屼簡瑙� 2锛�3-浜岀７閰哥敇娌归吀鏃佽矾锛屾帉鎻＄磪
绱�(x矛)鑳炲収(n猫i)绯栦唬璎濈殑鐢熺悊鎰忕京銆�
锛�3锛夋帉鎻¤绱呯礌鐨勭敓鐗╁悎鎴愬強鍏惰(di脿o)绡€(ji茅)锛岀悊瑙ｈ绱呰泲鐧界殑鍚堟垚銆�
锛�4锛夌悊瑙ｇ櫧绱�(x矛)鑳炵殑浠ｈ瑵銆�
17銆佽倽鐨勭敓鐗╁寲瀛�(xu茅)
鑰冭│鍏�(n猫i)瀹�
鑲濆湪鐗╄唱(zh矛)浠ｈ瑵涓殑浣滅敤 鑲濈殑鐢熺墿杞�(zhu菐n)鍖栦綔鐢� 鑶芥眮鑸囪喗姹侀吀浠ｈ瑵 鑶借壊绱�
鐨勪唬璎濊垏榛冪柛
鑰冭│瑕佹眰
锛�1锛変簡瑙ｈ倽鍦ㄧ硸銆佽剛銆佽泲鐧借唱(zh矛)銆佺董鐢熺礌鍜屾縺绱犱唬璎濅腑鐨勪綔鐢ㄣ€�
锛�2锛夋帉鎻＄敓鐗╄綁(zhu菐n)鍖栫殑姒傚康锛岀悊瑙ｇ敓鐗╄綁(zhu菐n)鍖栫殑鐢熺悊鎰忕京锛屾帉鎻¤倽鐨勭敓鐗╄綁(zhu菐n)鍖�
涓殑鍏╃浉鍙嶆噳(y墨ng)锛屼簡瑙ｇ敓鐗╄綁(zhu菐n)鍖栫殑瑾�(di脿o)绡€(ji茅)銆�
锛�3锛夌悊瑙ｈ喗姹佸拰鑶芥眮閰哥殑鍒嗛锛屾帉鎻¤喗姹侀吀鐨勪富瑕佺敓鐞嗗姛鑳斤紝鐞嗚В鑶芥眮閰�
鐨勪唬璎�锛屾帉鎻¤喗姹侀吀鐨勮倽鑵稿惊鐠�(hu谩n)鍙婂叾鐢熺悊鎰忕京銆�
锛�4锛夌悊瑙ｈ喗绱呯礌鐨勭敓鎴�锛屼簡瑙ｈ喗绱呯礌鐨勯亱杓搞€佽綁(zhu菐n)璁婂拰浠ｈ瑵杞�(zhu菐n)鍖�锛屾帉鎻¤喗绱�
绱犵殑鑲濊吀寰挵(hu谩n)锛岀悊瑙ｉ粌鐤稿強榛冪柛鐨勫垎椤�銆�
18銆佺董鐢熺礌鑸囩劇姗熼菇
鑰冭│鍏�(n猫i)瀹�
缍敓绱犵殑瀹氱京 鑴傛憾鎬х董鐢熺礌 姘存憾鎬х董鐢熺礌 閳�銆佺７浠ｈ瑵 寰噺鍏冪礌
鑰冭│瑕佹眰
锛�1锛夋帉鎻＄董鐢熺礌鐨勬蹇�锛岀悊瑙ｇ董鐢熺礌鐨勫垎椤炪€�
锛�2锛変簡瑙ｈ剛婧舵€х董鐢熺礌鐨勭壒榛�锛屾帉鎻＄董鐢熺礌 A銆佺董鐢熺礌 D 鐨勪締婧�銆佺ó椤�銆�
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[1] 鏌婚尗鑹富绶�. 鐢熺墿鍖栧(xu茅)锛堢涓冪増锛�. 浜烘皯琛�(w猫i)鐢熷嚭鐗堢ぞ, 2008
[2] 鍔夌兢鑹富绶�. 鐢熺墿鍖栧(xu茅). 鍖栧(xu茅)宸ユキ(y猫)鍑虹増绀�, 2011
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