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鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ(xu茅)鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗堬紝閮ㄥ垎瀛�(xu茅)鏍℃洿鏂拌嚦2012骞�锛�2013骞达紱鍧囨彁渚涙敹璨�(f猫i)涓嬭級銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛�(di菐n)鎿娾€�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛�(di菐n)鎿娾€�鑰冨崥瑭﹀嵎搴�鈥� 涓嬭級
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鑰冭│绉戠洰浠ｇ⒓锛歔 ] 鑰冭│绉戠洰鍚嶇ū锛氶啱(y墨)瀛�(xu茅)鍒嗗瓙鐢熺墿瀛�(xu茅)
涓€銆佽│鍗风祼(ji茅)妲�(g貌u)
1) 瑭﹀嵎鎴愮妇鍙婅€冭│鏅�(sh铆)闁�
鏈│鍗疯€冭│鏅�(sh铆)闁撶偤 180 鍒嗛悩銆�
2)绛旈鏂瑰紡锛氶枆鍗�銆佺瓎瑭�
3)瑭﹀嵎鍏�(n猫i)瀹圭祼(ji茅)妲�(g貌u)
閱�(y墨)瀛�(xu茅)鍒嗗瓙鐢熺墿瀛�(xu茅) 100%
4)椤屽瀷绲�(ji茅)妲�(g貌u)
a: 鍚嶈瑙ｉ噵锛�10 灏忛锛屽悇瑷�(j矛) 4%锛屽叡瑷�(j矛) 40%
b: 绨＄瓟椤�锛�5 灏忛锛屽悇瑷�(j矛) 6%锛屽叡瑷�(j矛) 30%
c: 鍟忕瓟椤岋紝3 灏忛锛屽悇瑷�(j矛) 10%锛屽叡瑷�(j矛) 30%
浜屻€佽€冭│鍏�(n猫i)瀹硅垏鑰冭│瑕佹眰
1銆佸熀鍥犲拰鍩哄洜绲�
鑰冭│鍏�(n猫i)瀹�
鍩哄洜鍜屽熀鍥犵祫鐨勬蹇� 鐥呮瘨鍩哄洜绲勭殑绲�(ji茅)妲�(g貌u)鑸囧姛鑳� 鍘熸牳鐢熺墿鍩哄洜绲� 鐪熸牳鐢�
鐗╁熀鍥犵祫 浜洪鍩哄洜绲勭殑鐗归粸(di菐n)鍜屼汉椤炲熀鍥犵祫瑷�(j矛)鍔� 鍩哄洜绲勫(xu茅) 鐢熺墿淇℃伅瀛�(xu茅)
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｅ熀鍥犲拰鍩哄洜绲勭殑姒傚康锛屾帉鎻″熀鍥犵殑绲�(ji茅)妲�(g貌u)銆�
锛�2锛夋帉鎻＄梾姣掑熀鍥犵祫鐨勭祼(ji茅)妲�(g貌u)鐗归粸(di菐n)锛岀悊瑙ｄ箼鑲濈梾姣掑拰鍙嶈綁(zhu菐n)閷勭梾姣掑熀鍥犵祫銆�
锛�3锛変簡瑙ｅ師鏍哥敓鐗╁熀鍥犵祫绲�(ji茅)妲�(g貌u)鑸囧姛鑳界壒榛�(di菐n)锛屾帉鎻¤唱(zh矛)绮掔殑姒傚康锛岀悊瑙ｈ唱(zh矛)绮掔殑鍒�
椤炲拰鐗规€�锛涙帉鎻¤綁(zhu菐n)搴т綔鐢ㄧ殑姒傚康锛岀悊瑙ｈ綁(zhu菐n)搴т綔鐢ㄧ殑姗�(j墨)鍒跺拰閬哄偝瀛�(xu茅)鏁堟噳(y墨ng)銆�
锛�4锛変簡瑙ｇ湡鏍哥敓鐗╁熀鍥犵祫鐨勭壒榛�(di菐n)锛屾帉鎻″熀鍥犲鏃忕殑姒傚康锛岀悊瑙ｅ熀鍥犲鏃忎腑鍚�
鍩哄洜闁撶殑闂�(gu膩n)绯诲拰鐗归粸(di菐n)銆�
锛�5锛変簡瑙ｄ汉椤炲熀鍥犵祫鐨勭壒榛�(di菐n)锛屾帉鎻′汉椤炲熀鍥犵祫瑷�(j矛)鍔冪殑鍏�(n猫i)瀹广€�
锛�6锛夋帉鎻″熀鍥犵祫瀛�(xu茅)鐨勬蹇�锛屼簡瑙ｅ熀鍥犵祫瀛�(xu茅)鐨勫収(n猫i)瀹癸紝鐞嗚В鍔熻兘鍩哄洜绲勫(xu茅)鐨勫収(n猫i)瀹�銆�
锛�7锛夋帉鎻＄敓鐗╀俊鎭(xu茅)鐨勬蹇�锛岀悊瑙ｇ敓鐗╀俊鎭(xu茅)鐨勪富瑕佹噳(y墨ng)鐢�銆�
2銆丏NA 搴忓垪鍒嗘瀽
鑰冭│鍏�(n猫i)瀹�
闆欒劔姘ф湯绔祩姝㈡硶 鍖栧(xu茅)闄嶈В娉� DNA 鑷嫊(d貌ng)娓簭
鑰冭│瑕佹眰
锛�1锛夋帉鎻￠洐鑴哀鏈绲傛娉曟脯搴忕殑鍩烘湰鍘熺悊鍜屼富瑕佹椹熴€�
锛�2锛夌悊瑙ｅ寲瀛�(xu茅)瑁傝В娉曟脯搴忕殑鍩烘湰鍘熺悊锛屾帉鎻″寲瀛�(xu茅)瑁傝В娉曟脯搴忕殑涓昏鎿嶄綔姝�
椹�銆�
锛�3锛夌悊瑙� DNA 鑷嫊(d貌ng)娓簭鐨勫熀鏈師鐞嗗拰涓昏鎳�(y墨ng)鐢�锛屼簡瑙ｆ脯搴忓湒璀滅殑闁辫畝鏂�
娉曘€�
3銆佹牳閰稿垎瀛愰洔浜�
鑰冭│鍏�(n猫i)瀹�
鏍搁吀鍒嗗瓙闆滀氦鐨勫師鐞� Southern 鍗拌贰闆滀氦 Northern 鍗拌贰闆滀氦 鏂戦粸(di菐n)闆滀氦
鍘熶綅闆滀氦 娑茬浉闆滀氦
鑰冭│瑕佹眰
锛�1锛夋帉鎻℃牳閰稿垎瀛愰洔浜ょ殑姒傚康锛岀悊瑙ｆ牳閰稿垎瀛愰洔浜ょ殑鍘熺悊銆�
锛�2锛夋帉鎻� Southern 鍗拌贰闆滀氦涓昏鎿嶄綔姝ラ锛屼簡瑙� Southern 鍗拌贰闆滀氦鐨勪富
瑕佹噳(y墨ng)鐢�銆�
锛�3锛夌悊瑙� Northern 鍗拌贰闆滀氦鐨勬搷浣滄椹熷拰涓昏鎳�(y墨ng)鐢ㄣ€�
锛�4锛夋帉鎻℃枒榛�(di菐n)闆滀氦鐨勬蹇靛拰鍘熺悊锛岀悊瑙ｆ枒榛�(di菐n)闆滀氦鐨勬搷浣滄椹熷拰涓昏鎳�(y墨ng)鐢�銆�
锛�5锛夋帉鎻″師浣嶉洔浜ょ殑姒傚康鍜屽師鐞�锛岀悊瑙ｅ師浣嶉洔浜ょ殑鎿嶄綔姝ラ鍜屼富瑕佹噳(y墨ng)鐢ㄣ€�
锛�6锛夋帉鎻℃恫鐩搁洔浜ょ殑姒傚康鍜屽師鐞�锛岀悊瑙ｆ牳閰搁叾 S1 淇濊(h霉)鍒嗘瀽娉� RNA 娌掍繚
璀�(h霉)鍒嗘瀽娉�銆佸紩鐗╁欢浼稿垎鏋愭硶鐨勫師鐞嗗拰涓昏鐨勬搷浣滄椹�銆�
锛�7锛夋帉鎻℃姂鍒舵秷娓涢洔浜ょ殑涓昏鎿嶄綔姝ラ锛岀悊瑙ｆ姂鍒舵秷娓涢洔浜ょ殑鍘熺悊鍜屼富瑕�
鎳�(y墨ng)鐢�
4銆佽仛鍚堥叾閺堝弽鎳�(y墨ng)
鑰冭│鍏�(n猫i)瀹�
鑱氬悎閰堕張鍙嶆噳(y墨ng)锛圥CR锛夌殑鍩烘湰鍘熺悊 鑰愮啽鐨� DNA 鑱氬悎閰� 寮曠墿瑷�(sh猫)瑷�(j矛)鐨勫熀鏈�
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PCR 澶氶噸 PCR
鑰冭│瑕佹眰
锛�1锛夌悊瑙� PCR 鐨勫畾缇�锛屾帉鎻� PCR 鐨勫師鐞嗗拰鍩烘湰閬庣▼锛屼簡瑙ｅ钩鑷�(t谩i)鏈熷拰骞宠嚭(t谩i)
鏁堟噳(y墨ng)銆�
锛�2锛変簡瑙ｈ€愮啽 DNA 鑱氬悎閰跺強鍏剁壒榛�(di菐n)銆�
锛�3锛夌悊瑙ｅ紩鐗╄ō(sh猫)瑷�(j矛)鐨勫熀鏈師鍓�銆�
锛�4锛夊(xu茅)鏈�(hu矛)鍎�(y艒u)鍖� PCR 鐨勫弽鎳�(y墨ng)姊濅欢銆�
锛�5锛夋帉鎻� RT-PCR 鐨勫師鐞�锛屼簡瑙ｅ叾涓昏鎳�(y墨ng)鐢ㄣ€�
锛�6锛夋帉鎻″(sh铆)鏅�(sh铆)鐔掑厜瀹氶噺 PCR 鐨勫師鐞�锛岀悊瑙ｅ叾涓昏鐨勬帰閲濇ā寮忓拰妾㈡脯鏂规硶锛�
浜嗚В鍏剁壒榛�(di菐n)鍜屼富瑕佹噳(y墨ng)鐢�銆�
锛�7锛夌悊瑙ｅ发寮� PCR 鍜屽閲� PCR 鐨勫師鐞嗗拰涓昏鎳�(y墨ng)鐢�銆�
锛�8锛夋帉鎻″師浣� PCR 鐨勬蹇靛拰鍘熺悊锛岀悊瑙ｅ叾鎿嶄綔鏂规硶鍜屼富瑕佹噳(y墨ng)鐢�銆�
5銆佸熀鍥犺姱鐗�
鑰冭│鍏�(n猫i)瀹�
鍩哄洜鑺墖鐨勫埗鍌� 鍩哄洜鑺墖鎶€琛�(sh霉) 鍩哄洜鑺墖鎶€琛�(sh霉)鐨勪富瑕佹噳(y墨ng)鐢�
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｅ熀鍥犺姱鐗囩殑涓昏鍒跺倷鏂规硶銆�.
锛�2锛夋帉鎻″熀鍥犺姱鐗囨妧琛�(sh霉)鐨勬娓師鐞嗗拰涓昏鎿嶄綔姝ラ銆�
锛�3锛夋帉鎻″熀鍥犺姱鐗囨妧琛�(sh霉)鐨勪富瑕佹噳(y墨ng)鐢�銆�
6銆佸厤鐤嵃璺℃妧琛�(sh霉)
鑰冭│鍏�(n猫i)瀹�
鍏嶇柅鍗拌贰鎶€琛�(sh霉)鐨勫師鐞� 鍏嶇柅鍗拌贰鎶€琛�(sh霉)鐨勬搷浣滄椹�
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｅ厤鐤嵃璺℃妧琛�(sh霉)鐨勫熀鏈師鐞嗭紝鎺屾彙鍏嶇柅鍗拌贰鎶€琛�(sh霉)鐨勪富瑕佹搷浣滄椹�锛�
浜嗚В鍏嶇柅鍗拌贰鎶€琛�(sh霉)鐨勪富瑕佹噳(y墨ng)鐢ㄣ€�
7銆佸熀鍥犵祼(ji茅)妲�(g貌u)鑸囪〃閬�(d谩)鍒嗘瀽鐨勫叾浠栨妧琛�(sh霉)
鑰冭│鍏�(n猫i)瀹�
cDNA 鏈蹇€熸摯(ku貌)澧炴妧琛�(sh霉) PCR-SSCP PCR-RFLP
鑰冭│瑕佹眰
锛�1锛夌悊瑙� cDNA 鏈蹇€熸摯(ku貌)澧炴妧琛�(sh霉)鐨勫師鐞�锛屾帉鎻� cDNA 鏈蹇€熸摯(ku貌)澧炴妧琛�(sh霉)
鐨勪富瑕佹搷浣滄椹�锛屼簡瑙� cDNA 鏈蹇€熸摯(ku貌)澧炴妧琛�(sh霉)鐨勪富瑕佹噳(y墨ng)鐢�銆�
锛�2锛夌悊瑙� PCR-SSCP 鐨勫師鐞�锛屾帉鎻� PCR-SSCP 鐨勪富瑕佹搷浣滄椹�锛屼簡瑙�
PCR-SSCP 鐨勪富瑕佹噳(y墨ng)鐢�銆�
锛�3锛夌悊瑙� PCR-RFLP 鐨勫師鐞�锛屾帉鎻� PCR-RFLP 鐨勪富瑕佹搷浣滄椹�锛屼簡瑙�
PCR-RFLP 鐨勪富瑕佹噳(y墨ng)鐢ㄣ€�
8銆佸熀鍥犲伐绋嬭垏楂斿琛ㄩ仈(d谩)
鑰冭│鍏�(n猫i)瀹�
鍩哄洜鍏嬮殕鐨勫伐鍏烽叾 鍩哄洜鍏嬮殕鐨勮級楂� 鍩哄洜鍏嬮殕鐨勫熀鏈亷绋� 鐪熸牳绱�(x矛)鑳炵殑
杞�(zhu菐n)鏌� 鍩哄洜鐨勬敼閫� 鍏嬮殕鍩哄洜鐨勮〃閬�(d谩)
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｅ熀鍥犲厠闅嗗拰鍩哄洜宸ョ▼鐨勬蹇�锛屼簡瑙ｅ熀鍥犲厠闅嗙殑鐩殑銆�
锛�2锛夌悊瑙ｉ檺鍒舵€ф牳閰稿収(n猫i)鍒囬叾鐨勫垎椤�銆佷綔鐢ㄧ壒榛�(di菐n)鍜屼富瑕佹噳(y墨ng)鐢紝浜嗚В闄愬埗鎬ф牳
閰稿収(n猫i)鍒囬叾鐨勫懡鍚�銆�
锛�3锛夌悊瑙ｈ級楂旂殑姒傚康锛屾帉鎻″厠闅嗚級楂斿拰琛ㄩ仈(d谩)杓夐珨鐨勪富瑕佹(g貌u)浠跺拰鐗归粸(di菐n)锛屼簡瑙�
涓昏鐨勫厠闅嗚級楂斿拰琛ㄩ仈(d谩)杓夐珨銆�
锛�4锛夋帉鎻″熀鍥犲厠闅嗙殑鍩烘湰姝ラ锛岀悊瑙ｇ洰鐨勫熀鍥犵嵅鍙栫殑涓昏鏂规硶鍙婇噸绲勫瓙鐨�
绡╅伕鍜岄憭瀹氱殑涓昏鏂规硶锛屾帉鎻¤綁(zhu菐n)鍖�銆佽綁(zhu菐n)鏌撳拰鎰熸煋鐨勬蹇点€�
锛�5锛夋帉鎻＄湡鏍哥窗(x矛)鑳炶綁(zhu菐n)鏌撶殑涓昏鏂规硶锛岀悊瑙ｈ綁(zhu菐n)鏌撶殑鐪熸牳绱�(x矛)鑳炵閬哥殑涓昏鏂规硶銆�
锛�6锛夋帉鎻″熀鍥犲畾榛�(di菐n)瑾樿畩鐨勪富瑕佹柟娉�銆備簡瑙ｅ熀鍥犲畾榛�(di菐n)瑾樿畩鐨勪富瑕佹噳(y墨ng)鐢�銆�
锛�7锛夌悊瑙ｅ厠闅嗗熀鍥犲師鏍歌〃閬�(d谩)鐨勫熀鏈绱犲拰鎻愰珮琛ㄩ仈(d谩)姘村钩鐨勪富瑕佹帾鏂�锛屼簡瑙�
鍕�(d貌ng)鐗╃窗(x矛)鑳炶〃閬�(d谩)绯荤当(t菕ng)銆佹槅锜茬窗(x矛)鑳炶〃閬�(d谩)绯荤当(t菕ng)鍜岄叺姣嶈〃閬�(d谩)绯荤当(t菕ng)銆�
9銆佽泲鐧借唱(zh矛)绲勫(xu茅)
鑰冭│鍏�(n猫i)瀹�
顥ヮ棩铔嬬櫧璩�(zh矛)绲勫(xu茅)鐨勬蹇靛強鐧�(f膩)灞曞彶 铔嬬櫧璩�(zh矛)绲勮〃閬�(d谩)鐨勭爺绌舵柟娉� 铔嬬櫧璩�(zh矛)鐩镐簰浣滅敤
鐨勭爺绌舵柟娉�
鑰冭│瑕佹眰
锛�1锛夋帉鎻¤泲鐧借唱(zh矛)绲勫強铔嬬櫧璩�(zh矛)绲勫(xu茅)鐨勬蹇�锛屼簡瑙ｈ泲鐧借唱(zh矛)绲勫(xu茅)鐨勭櫦(f膩)灞曞彶銆�
锛�2锛夋帉鎻¤泲鐧借唱(zh矛)绲勮〃閬�(d谩)妯″紡鐮旂┒鐨勫熀鏈椹�锛岀悊瑙ｅ畾閲忚泲鐧借唱(zh矛)绲勭爺绌剁殑绛�
鐣�銆�
锛�3锛夌悊瑙ｉ叺姣嶉洐闆滀氦绯荤当(t菕ng)鐨勫熀鏈師鐞嗗拰涓昏鐨勬搷浣滄椹�銆�
锛�4锛夌悊瑙ｅ櫖鑿岄珨琛ㄩ潰灞曠ず鎶€琛�(sh霉)鐨勫熀鏈師鐞嗗拰涓昏鐨勬搷浣滄椹�銆�
10銆佸熀鍥犺綁(zhu菐n)绉绘妧琛�(sh霉)鍜屽熀鍥犳墦鍚ф妧琛�(sh霉)
鑰冭│鍏�(n猫i)瀹�
顥ヮ棩顥ヨ綁(zhu菐n)鍩哄洜鍕�(d貌ng)鐗� 鍕�(d貌ng)鐗╄綁(zhu菐n)鍩哄洜鐨勫熀鏈師鐞� 鍕�(d貌ng)鐗╄綁(zhu菐n)鍩哄洜鐨勫熀鏈柟娉� 鍩哄洜鎵�
闈舵妧琛�(sh霉) 鍩哄洜淇＞鍕�(d貌ng)鐗╃殑鎳�(y墨ng)鐢�
鑰冭│瑕佹眰
顥ワ紙1锛夌悊瑙ｈ綁(zhu菐n)鍩哄洜鍕�(d貌ng)鐗�銆佸熀鍥犲墧闄�銆佸熀鍥犻崶鍏ョ殑姒傚康銆�
锛�2锛夋帉鎻″嫊(d貌ng)鐗╄綁(zhu菐n)鍩哄洜鎶€琛�(sh霉)鐨勫熀鏈師鐞嗭紝鐞嗚В鍕�(d貌ng)鐗╄綁(zhu菐n)鍩哄洜鎶€琛�(sh霉)鐨勫熀鏈柟娉�锛屾帉
鎻¤綁(zhu菐n)鍩哄洜鍕�(d貌ng)鐗╃殑妾㈡脯銆�
锛�3锛夋帉鎻″熀鍥犳墦闈剁殑姒傚康锛岀悊瑙ｅ熀鍥犳墦闈剁殑鍩烘湰鍘熺悊锛屼簡瑙ｅ熀鍥犳墦闈剁殑鍩烘湰
鏂规硶銆�
锛�4锛変簡瑙ｅ熀鍥犱慨椋惧嫊(d貌ng)鐗╁湪閱�(y墨)瀛�(xu茅)涓婄殑鎳�(y墨ng)鐢�銆�
11銆佸熀鍥犺ê鏂�
鑰冭│鍏�(n猫i)瀹�
鍩哄洜瑷烘柗鐨勬蹇� 鍩哄洜瑷烘柗鐨勬妧琛�(sh霉) 鍩哄洜瑷烘柗鐨勬柟娉� 閬哄偝鐥呯殑鍩哄洜瑷烘柗
鍌虫煋鐥呯殑鍩哄洜瑷烘柗 鑵槫鐨勫熀鍥犺ê鏂� 鍩哄洜瑷烘柗鍦ㄦ硶閱�(y墨)瀛�(xu茅)涓婄殑鎳�(y墨ng)鐢�
鑰冭│瑕佹眰
锛�1锛夌悊瑙ｅ熀鍥犺ê鏂风殑姒傚康锛屾帉鎻″熀鍥犺ê鏂风殑鐗归粸(di菐n)銆�
锛�2锛夌悊瑙ｅ熀鍥犺ê鏂峰父鐢ㄧ殑鍒嗗瓙鐢熺墿瀛�(xu茅)鎶€琛�(sh霉)銆�
锛�3锛変簡瑙ｅ熀鍥犺ê鏂风殑鎶€琛�(sh霉)璺窔锛屾帉鎻＄洿鎺ュ熀鍥犺ê鏂烽€斿緫鍜岄枔鎺ュ熀鍥犺ê鏂烽€�
寰戠殑涓昏鏂规硶銆�
锛�4锛変簡瑙ｄ富瑕佺殑閬哄偝鐥�锛岀悊瑙ｅ叾鍩哄洜瑷烘柗鐨勪富瑕佹柟娉�銆�
锛�5锛変簡瑙ｄ富瑕佺殑鍌虫煋鐥�锛岀悊瑙ｅ叾鍩哄洜瑷烘柗鐨勪富瑕佹柟娉�銆�
锛�6锛変簡瑙ｅ師鐧屽熀鍥犲拰鎶戠檶鍩哄洜鐨勬娓強鍏跺湪鑵槫瑷烘柗涓殑鎳�(y墨ng)鐢�銆�
锛�7锛夋帉鎻� DNA 鎸囩磱鐨勬蹇碉紝鐞嗚В DNA 鎸囩磱鍒嗘瀽鐨勫熀鏈祦绋�锛屼簡瑙� DNA
鎸囩磱鍦ㄦ硶閱�(y墨)瀛�(xu茅)涓婄殑鎳�(y墨ng)鐢�銆�
12銆佸熀鍥犳不鐧�
鑰冭│鍏�(n猫i)瀹�:
鍩哄洜娌荤檪鐨勬蹇� 鍩哄洜娌荤檪鐨勫熀鏈瓥鐣� 鍩哄洜杞�(zhu菐n)绉荤殑鍩烘湰鎶€琛�(sh霉) 鍩哄洜杞�(zhu菐n)绉�
鐨勯澏绱�(x矛)鑳� 鍩哄洜骞查爯(y霉)
鑰冭│瑕佹眰
锛�1锛夋帉鎻″熀鍥犳不鐧傜殑姒傚康銆�
锛�2锛夌悊瑙ｅ熀鍥犳不鐧傜殑鍩烘湰绛栫暐銆�
锛�3锛夋帉鎻＄梾姣掍粙灏�(d菐o)鐨勫熀鍥犺綁(zhu菐n)绉荤郴绲�(t菕ng)锛岀悊瑙ｈ剛璩�(zh矛)楂斾粙灏�(d菐o)鐨勫熀鍥犺綁(zhu菐n)绉绘妧琛�(sh霉)锛屼簡
瑙ｅ彈楂斾粙灏�(d菐o)鐨勫熀鍥犺綁(zhu菐n)绉绘妧琛�(sh霉)鍜屽熀鍥犵洿鎺ユ敞灏勬妧琛�(sh霉)銆�
锛�4锛変簡瑙ｅ熀鍥犺綁(zhu菐n)绉荤殑甯哥敤闈剁窗(x矛)鑳�銆�
锛�5锛夋帉鎻� RNA 骞叉摼鎶€琛�(sh霉)鐨勬蹇靛拰鍘熺悊锛岀悊瑙ｅ弽缇� RNA 鎶€琛�(sh霉)鐨勫師鐞�锛屼簡瑙�
鏍搁叾鎶€琛�(sh霉)鐨勫師鐞嗗拰鎳�(y墨ng)鐢�銆�
13銆佽叓鐦ゅ垎瀛愮敓鐗╁(xu茅)
鑰冭│鍏�(n猫i)瀹�:
鑵槫鐧�(f膩)鐢熺殑鍒嗗瓙姗�(j墨)鍒� 鐧屽熀鍥� 鎶戠檶鍩哄洜
鑰冭│瑕佹眰:
锛�1锛夌悊瑙ｈ叓鐦ょ櫦(f膩)鐢熺殑鍒嗗瓙姗�(j墨)鍒�銆�
锛�2锛夋帉鎻＄檶鍩哄洜鐨勬蹇�锛岀悊瑙ｅ師鐧屽熀鍥犳縺娲荤殑姗�(j墨)鍒讹紝浜嗚В涓昏鐨勭檶鍩哄洜锛�
鐔熸倝鐧屽熀鍥犵殑鍒嗛銆�
锛堬紙2锛夋帉鎻℃姂鐧屽熀鍥犵殑姒傚康锛岀悊瑙ｄ富瑕佺殑鎶戠檶鍩哄洜鍙婂叾浣滅敤姗�(j墨)鍒讹紝浜嗚В鎶戠檶
鍩哄洜澶辨椿鐨勬(j墨)鍒�銆�
涓�銆佸弮鑰冩浉鐩�
[1] 鑳＄董鏂颁富绶�. 閱�(y墨)瀛�(xu茅)鍒嗗瓙鐢熺墿瀛�(xu茅). 绉戝(xu茅)鍑虹増绀�, 2007
[2] 棣綔鍖栦富绶�. 閱�(y墨)瀛�(xu茅)鍒嗗瓙鐢熺墿瀛�(xu茅). 浜烘皯琛�(w猫i)鐢熷嚭鐗堢ぞ, 2005
鍏嶈铂(z茅)鑱叉槑锛氭湰鏂囩郴杞�(zhu菐n)杓夎嚜缍�(w菐ng)绲�(lu貌)锛屽鏈変镜鐘紝璜�(q菒ng)鑱�(li谩n)绯绘垜鍊戠珛鍗冲埅闄�锛屽彟锛氭湰鏂囧儏浠ｈ〃浣滆€呭€�(g猫)浜鸿榛�(di菐n)锛岃垏鏈恫(w菐ng)绔欑劇闂�(gu膩n)銆傚叾鍘熷壍(chu脿ng)鎬т互鍙婃枃涓櫝杩版枃瀛楀拰鍏�(n猫i)瀹规湭缍�(j墨ng)鏈珯璀夊(sh铆)锛屽皪(du矛)鏈枃浠ュ強鍏朵腑鍏ㄩ儴鎴栬€呴儴鍒嗗収(n猫i)瀹�銆佹枃瀛楃殑鐪熷(sh铆)鎬�銆佸畬鏁存€�銆佸強鏅�(sh铆)鎬ф湰绔欎笉浣滀换浣曚繚璀夋垨鎵胯锛岃珛(q菒ng)璁€鑰呭儏浣滃弮鑰�锛屽苟璜�(q菒ng)鑷鏍稿(sh铆)鐩搁棞(gu膩n)鍏�(n猫i)瀹�銆�
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鏂囩珷鎼滅储
鎮ㄧ従(xi脿n)鍦ㄧ殑浣嶇疆锛� 
