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鑰冭│瑕佹眰: (1)鎺屾彙閬哄偝鐗╄唱(zh矛)鐨勬蹇靛強鍒嗛銆傜啛绶存帉鎻℃牳閰哥殑鍖栧(xu茅)绲勬垚銆佷竴绱�(j铆)绲�(ji茅)妲�(g貌u)銆佷簩 绱�(j铆)绲�(ji茅)妲�(g貌u)鍜岄珮绱�(j铆)绲�(ji茅)妲�(g貌u);鎺屾彙鐪熸牳鐢熺墿鏌撹壊楂旂殑鍖栧(xu茅)绲勬垚銆佺祫瑁濆堡娆″強鍏堕珮绱�(j铆)绲�(ji茅)妲�(g貌u)鍜� 鍔熻兘銆� (2)鐔熺反鎺屾彙 RNA 鐨勭祼(ji茅)妲�(g貌u)鐗归粸(di菐n)锛岃垏 DNA 鐨勫崁(q奴)鍒ュ強 RNA 鐨勫垎椤炲拰澶氭ǎ鎬�銆� (3)鐔熺反鎺屾彙鏍搁吀鐨勮畩鎬с€佸京(f霉)鎬ц垏闆滀氦銆� 3銆佸熀鍥犺垏鍩哄洜绲勭殑绲�(ji茅)妲�(g貌u)鑸囧姛鑳� 鑰冭│鍏�(n猫i)瀹�: 鍩哄洜鑸囧熀鍥犵祫鐨勬蹇�銆佺湡鏍哥敓鐗╁熀鍥犵祫鐨勫ぇ灏忓強鍏惰垏 C 鍊肩殑闂�(gu膩n)绯�;鐥呮瘨鍙婂叾 鍩哄洜绲�锛涚窗(x矛)鑿屽熀鍥犵祫锛涚湡鏍哥敓鐗╁熀鍥犵祫;鍚屾簮閲嶇祫鍜屼綅榛�(di菐n)鐗圭暟鎬ч噸绲�;杞�(zhu菐n)搴т綔鐢ㄥ拰 閫嗚綁(zhu菐n)閷勮綁(zhu菐n)搴у瓙銆� 鑰冭│瑕佹眰: (1) 鐔熺反鎺屾彙鍩哄洜鑸囧熀鍥犵祫鐨勬蹇碉紝鐔熸倝鐪熸牳鐢熺墿鍩哄洜绲勭殑澶у皬鍙婂叾鑸� C 鍊肩殑闂�(gu膩n)绯�銆� (2) 鐔熺反鎺屾彙绱�(x矛)鑿屽熀鍥犵祫鍜岀湡鏍哥敓鐗╁熀鍥犵祫鐨勭祼(ji茅)妲�(g貌u)銆佺壒榛�(di菐n)銆� (3)鐔熸倝绶氱矑楂斿拰钁夌稜楂斿熀鍥犲強鍏剁祼(ji茅)妲�(g貌u)鐗瑰緛銆� (4)浜嗚В浜洪鍩哄洜绲�銆佽泲鐧界祫鍙婄敓鐗╀俊鎭(xu茅)銆� (5)鐔熺反鎺屾彙鍚屾簮閲嶇祫鐨勫垎瀛愭ā寮忋€佸熀鏈蹇佃垏閰剁郴銆� (6)鎺屾彙鍣弻楂斿拰澶ц吀妗胯弻浣嶉粸(di菐n)鐗圭暟鎬ч噸绲勩€� (7)鐔熺反鎺屾彙杞�(zhu菐n)搴у瓙鐨勬蹇�銆佸垎椤炲強杞�(zhu菐n)搴ф(j墨)鐞嗗拰鐗瑰緛锛屾帉鎻¢€嗚綁(zhu菐n)閷勮綁(zhu菐n)搴у瓙鐨� 绲�(ji茅)妲�(g貌u)鐗归粸(di菐n)銆佹(j墨)鐞�;鐞嗚В杞�(zhu菐n)搴у拰閫嗚綁(zhu菐n)閷勮綁(zhu菐n)搴х殑閬哄偝鏁堟噳(y墨ng)鍜岀敓鐗╁(xu茅)鎰忕京銆� 4銆丏NA 寰�(f霉)鍒躲€佽綁(zhu菐n)閷勮垏杞�(zhu菐n)閷勫悗鐨勫壀鎺ヨ垏鍔犲伐 鑰冭│鍏�(n猫i)瀹癸細 DNA 寰�(f霉)鍒舵杩�;澶ц吀妗胯弻 DNA 寰�(f霉)鍒跺強鍏惰(di脿o)鎺�銆佺湡鏍哥敓鐗� DNA 寰�(f霉)鍒跺強鍏惰(di脿o)鎺�; 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铔嬬櫧璩�(zh矛)鍚堟垚鐨勫垎瀛愬熀绀�(ch菙)锛屽悎鎴愰亷(gu貌)绋嬭垏瑾�(di脿o)鎺�;铔嬬櫧璩�(zh矛)鐨勮綁(zhu菐n)閬�(y霉n);铔嬬櫧璩�(zh矛)鐨勪慨椋捐垏鎶樼枈銆� 鑰冭│瑕佹眰: (1)浜嗚В瀵嗙⒓鐨勫熀鏈壒寰�锛屾帉鎻″瘑纰煎瓙鐨勭啊(ji菐n)骞舵€у拰璁婂伓鎬с€侀€氱敤鎬у拰璁婄暟鍨�銆� (2)鎺屾彙璧峰瀵嗙⒓鍜岀祩姝㈠瘑纰硷紝鐔熺反鎺屾彙铔嬬櫧璩�(zh矛)鐨勭炕璀亷(gu貌)绋嬪強铔嬬櫧璩�(zh矛)鐨勫悎鎴� 瑾�(di脿o)鎺�銆� (3) 鐔熸倝铔嬬櫧璩�(zh矛)鐨勪慨椋�銆佹姌鐤婂強铔嬬櫧璩�(zh矛)鍚堟垚鍚庣殑閬�(y霉n)杓�銆� 7.鍩哄洜琛ㄩ仈(d谩)鑸囪(di脿o)鎺� 鑰冭│鍏�(n猫i)瀹癸細 鍘熸牳鍩哄洜琛ㄩ仈(d谩)鑸囪(di脿o)鎺�锛涙銆佽矤(f霉)瑾�(di脿o)鎺х郴绲�(t菕ng)锛涗钩绯栨搷绺卞瓙銆佽壊姘ㄩ吀鎿嶇副瀛�銆侀樋鎷� 浼硸鎿嶇副瀛�銆佸崐涔崇硸鎿嶇副瀛�銆佺祫姘ㄩ吀鎿嶇副瀛�锛涚窗(x矛)鑿岀殑鎳�(y墨ng)鎬ュ弽鎳�(y墨ng)銆傜湡鏍哥窗(x矛)鑳炲熀鍥犺〃 閬�(d谩)瑾�(di脿o)鎺х殑鐗归粸(di菐n)鑸囦笉鍚屽堡娆�锛涚湡鏍哥敓鐗╄綁(zhu菐n)閷勮(di脿o)鎺х殑鍚勭ó闋嗗紡浣滅敤鍏冧欢鍜屽弽寮忎綔鐢ㄥ洜 瀛�銆� 鑰冭│瑕佹眰: (1)鎺屾彙鍘熸牳鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺х殑鐗归粸(di菐n)锛岀啛绶存帉鎻′钩绯栨搷绺卞瓙瀛�(xu茅)瑾�(shu艒)鍙婅(di脿o)鎺ф(j墨)鐞�銆� 鐔熸倝鍏跺畠鍚勬搷绺卞瓙鐨勮(di脿o)鎺ф(j墨)鍒�銆� (2)鎺屾彙鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺х殑鍩烘湰姒傚康锛屾銆佽矤(f霉)瑾�(di脿o)鎺х郴绲�(t菕ng)銆� (3)鎺屾彙鐪熸牳鍩哄洜琛ㄩ仈(d谩)鐨勪笉鍚屽堡娆″強鍏惰(di脿o)鎺�;鍚勭ó闋嗗紡浣滅敤鍏冧欢鐨勬蹇靛拰鐗� 榛�(di菐n)浠ュ強鍙嶅紡浣滅敤鍥犲瓙鐨勯鍨嬭垏绲�(ji茅)妲�(g貌u)銆� 8.鍩哄洜宸ョ▼鍜岃泲鐧借唱(zh矛)宸ョ▼ 鑰冭│鍏�(n猫i)瀹癸細 DNA 鍏嬮殕鐨勫熀鏈師鐞�锛涘吀鍨嬬殑閬哄偝宸ョ▼鎶€琛�(sh霉)锛涜級楂旀敼閫犲師鐞�锛涘熀鍥犱締(l谩i)婧愩€佷汉 椤炲熀鍥犲伐绋嬭▓(j矛)鍔冨強鏍哥畻闋嗗簭鍒嗘瀽锛汻NA 鍜� DNA 鐨勬脯(c猫)搴忔柟娉曞強鍏堕亷(gu貌)绋�锛涘熀鍥犵殑鍒嗛洟銆� 鍚堟垚鍜屾脯(c猫)搴�锛涜泲鐧借唱(zh矛)宸ョ▼銆� 鑰冭│瑕佹眰: (1)鐞嗚В DNA 鍏嬮殕鐨勫熀鏈師鐞�銆� (2)鎺屾彙鍚勭ó姘村钩涓婄殑鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺�銆� (3)浜嗚В浜洪鍩哄洜绲勮▓(j矛)鍔冨強鏍哥畻搴忓垪鍒嗘瀽銆� (4)鎺屾彙 RNA 鍜� DNA 鐨勬脯(c猫)搴忔柟娉曞強鍏堕亷(gu貌)绋嬨€� (5)浜嗚В铔嬬櫧璩�(zh矛)宸ョ▼鐨勯€�(j矛n)灞�銆� 9.鍒嗗瓙鐢熺墿瀛�(xu茅)瀵�(sh铆)椹�(y脿n)鎶€琛�(sh霉) 鑰冭│鍏�(n猫i)瀹癸細 鍒嗗瓙鐢熺墿瀛�(xu茅)瀵�(sh铆)椹�(y脿n)鍩烘湰鎶€琛�(sh霉)锛涘垎瀛愬厠闅嗘妧琛�(sh霉)鍖呮嫭 PCR 瀵�(sh铆)椹�(y脿n)鎶€琛�(sh霉)銆侀檺鍒舵€у収(n猫i)鍒� 閰堕叾鍒�銆佸熀鍥犻噸绲勩€佽級楂旂殑鎬ц唱(zh矛)锛涙牳閰哥殑鎻愬彇锛涘熀鍥犵殑鍒嗛洟鑸囬噸绲勯憭瀹氾紱鍩哄洜鏂� 搴�(k霉)鐨勬(g貌u)寤�锛涘垎瀛愰洔浜�锛汥NA 娓�(c猫)搴忕殑鍘熺悊锛涚従(xi脿n)浠e垎瀛愮敓鐗╁(xu茅)鐔遍粸(di菐n)鎶€琛�(sh霉)銆� 鑰冭│瑕佹眰: (1) 鎺屾彙鍩烘湰瀵�(sh铆)椹�(y脿n)鎶€琛�(sh霉)鐨勫(sh铆)椹�(y脿n)鍘熺悊銆� (2) 鐔熸倝甯哥敤瀵�(sh铆)椹�(y脿n)鐨勫熀鏈亷(gu貌)绋嬪強瑕侀粸(di菐n)銆� 涓夈€佸弮鑰冩浉鐩� 1锛庛€婂垎瀛愮敓鐗╁(xu茅)銆嬶紙绗� 3 鐗堬級锛堜腑璀増锛� [Molecular Biology (Third Edition)]锛� 鐗圭磵锛圥hil Turner锛�锛孉lexander Mclennan锛孉ndy Bates 绛夎憲锛涘妷閫�(j矛n)鍏�锛� 鍔夋枃绌庤銆傜簿瑕侀€熻绯诲垪銆� 2.銆婄従(xi脿n)浠e垎瀛愮敓鐗╁(xu茅)銆�(绗洓鐗�)锛屾湵鐜夎尝 鏉庢瘏钁楋紝楂樼瓑鏁欒偛鍑虹増绀�锛�2012銆�
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