|
鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�(gu贸)400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ(xu茅)鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗�锛岄儴鍒嗗(xu茅)鏍℃洿鏂拌嚦2012骞�锛�2013骞�锛涘潎鎻愪緵鏀惰不(f猫i)涓嬭級銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛�(di菐n)鎿娾€�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛�(di菐n)鎿娾€�鑰冨崥瑭﹀嵎搴�鈥� 涓嬭級
婀栧崡甯寖澶у(xu茅)纰╁＋鐮旂┒鐢熷叆瀛�(xu茅)鑰冭│鑷懡椤岃€冭│澶х侗
鍚岀瓑瀛�(xu茅)鍔涘姞瑭︾鐩� 绉戠洰鍚嶇ū锛氱従(xi脿n)浠ｅ垎瀛愮敓鐗╁(xu茅)
涓€銆佽€冭│褰㈠紡鑸囪│鍗风祼(ji茅)妲�(g貌u)
1锛� 瑭﹀嵎鎴愮妇(j墨)鍙婅€冭│鏅�(sh铆)闁擄細
鏈│鍗锋豢鍒嗙偤 100 鍒�锛涜€冭│鏅�(sh铆)闁撶偤 180 鍒嗛悩銆�
2锛� 绛旈鏂瑰紡锛�
闁夊嵎銆佺瓎瑭︺€�
3锛� 瑭﹀嵎鍏�(n猫i)瀹圭祼(ji茅)妲�(g貌u)
锛堜竴锛夊熀绀�(ch菙)鐭ヨ瓨(sh铆)閮ㄥ垎 80%
锛堜簩锛夌爺绌堕€�(j矛n)灞�銆佸嫊(d貌ng)鎱�(t脿i)閮ㄥ垎 20%
4锛� 椤屽瀷绲�(ji茅)妲�(g貌u)
锛堜竴锛夊悕绋辫В閲嬮锛�10 灏忛锛屾瘡灏忛 2 鍒嗭紝鍏� 20 鍒�
锛堜簩锛夊垽鏂烽锛�10 灏忛锛� 姣忓皬椤� 1 鍒�锛屽叡 10 鍒�
锛堜笁锛夊～绌洪锛�30 绌�锛屾瘡绌� 0.5 鍒�锛� 鍏� 15 鍒�
锛堝洓锛夊柈闋�(xi脿ng)閬告搰椤岋細15 灏忛锛屾瘡灏忛 1 鍒�锛屽叡 15 鍒�
锛堜簲锛夌啊(ji菐n)绛旈锛�5-8 椤�锛� 鍏� 40 鍒�
浜�銆佽€冭│鍏�(n猫i)瀹硅垏鑰冭│瑕佹眰
锛堜竴锛夎€冭│瑕佹眰
鎺屾彙鐝�(xi脿n)浠ｅ垎瀛愮敓鐗╁(xu茅)鐨勬蹇靛拰涓昏鐮旂┒?j墨)?n猫i)瀹癸紝浜嗚В鐝�(xi脿n)浠ｅ垎瀛愮敓鐗╁(xu茅)鐮旂┒鐨勬柊
閫�(j矛n)灞�锛涢噸榛�(di菐n)鎶婃彙 DNA銆丷NA 鍜岃泲鐧借唱(zh矛)绛夌敓鐗╁ぇ鍒嗗瓙鐨勫熀鏈敓鐗╁寲瀛�(xu茅)鐗规€т互鍙婄祼(ji茅)妲�(g貌u)
鑸囧姛鑳界殑闂�(gu膩n)绯伙紝鐢熺墿淇℃伅鐨勫劜(ch菙)瀛�銆佸偝閬炶垏琛ㄩ仈(d谩)閬庣▼锛屽熀鍥犺〃閬�(d谩)鐨勮(di脿o)鎺у師鐞�锛屽垎瀛�
鐢熺墿瀛�(xu茅)鐨勫熀鏈爺绌舵柟娉曪紱鍒濇鐞嗚В鍩哄洜瑾�(di脿o)鎺ц垏鐢熺墿鐧�(f膩)鑲�銆佸厤鐤拰鐤剧梾鐧�(f膩)鐢熺殑闂�(gu膩n)绯汇€�
锛堜簩锛夎€冭│鍏�(n猫i)瀹�
1銆佺敓鐗╁ぇ鍒嗗瓙鐨勬€ц唱(zh矛)銆佺祼(ji茅)妲�(g貌u)鍙婄祼(ji茅)妲�(g貌u)鑸囧姛鑳界殑闂�(gu膩n)绯�
20 绋富瑕佹皑鍩洪吀鐨勭ó椤炲拰绲�(ji茅)妲�(g貌u)锛岃偨閸靛拰鑲界殑姒傚康锛岃泲鐧借唱(zh矛)鐨勪竴绱�(j铆)绲�(ji茅)妲�(g貌u)鍜岄珮
绱�(j铆)绲�(ji茅)妲�(g貌u)鐨勬蹇�銆佷富瑕佽〃鐝�(xi脿n)褰㈠紡鑸囩董绯诲寲瀛�(xu茅)閸碉紝铔嬬櫧璩�(zh矛)鐨勭悊鍖栨€ц唱(zh矛)锛堝叐鎬цВ闆�銆佽啝
楂旀€ц唱(zh矛)銆佹矇娣€銆佽畩鎬с€佸嚌鍥�銆佺传澶栧惛鏀剁壒寰佸拰鍛堣壊鍙嶆噳(y墨ng)锛�锛岃泲鐧借唱(zh矛)绲�(ji茅)妲�(g貌u)鍜屽姛鑳界殑
闂�(gu膩n)绯伙紝铔嬬櫧璩�(zh矛)璁婃€ц垏寰�(f霉)鎬�锛屽垎闆�銆佺磾鍖栥€佹脯(c猫)瀹氳泲鐧借唱(zh矛)鐨勪竴鑸師鐞嗚垏鏂规硶锛涙牳鑻烽吀
鐨勭祼(ji茅)妲�(g貌u)锛�5 绋富瑕佸牽鍩鸿垏 2 绋牳绯栵紝鏍搁吀鐨勪竴绱�(j铆)绲�(ji茅)妲�(g貌u)锛孌NA 鐨勭┖闁撶祼(ji茅)妲�(g貌u)鑸囧姛鑳斤紙涓�
瑕佹槸闆欒灪鏃嬬祼(ji茅)妲�(g貌u)妯″瀷锛夛紝RNA 鐨勭ó椤�銆佺┖闁撶祼(ji茅)妲�(g貌u)鑸囧姛鑳斤紝鏍搁吀鐨勭悊鍖栨€ц唱(zh矛)锛堢传澶�
鍚告敹銆佽畩鎬с€佸京(f霉)鎬у拰闆滀氦銆佹按瑙ｏ級锛屽垎闆�銆佺磾鍖�銆佹脯(c猫)瀹氭牳閰哥殑涓€鑸師鐞嗚垏鏂规硶銆�
2銆佺敓鐗╀俊鎭殑鍌抽仦
閬哄偝瀵嗙⒓锛堜笁鑱�(li谩n)楂旈伜鍌冲瘑纰肩殑姒傚康锛岄伜鍌冲瘑纰肩殑鐗规€э級锛屽熀鍥犵殑姒傚康鍜屽垎瀛�
灞€э紝鏌撹壊楂旂殑绲勬垚鑸囩祼(ji茅)妲�(g貌u)锛堜富瑕佹槸鏍稿皬楂旂殑绲�(ji茅)妲�(g貌u)鍜岃秴铻烘棆绲�(ji茅)妲�(g貌u)锛�锛孌NA 鐨勫京(f霉)鍒�
鍜岀壒榛�(di菐n)锛孌NA 鐨勪慨寰�(f霉)锛屽熀鍥犵殑鍩烘湰绲�(ji茅)妲�(g貌u)锛堢壒鍒ユ槸鍟熷嫊(d貌ng)瀛愮殑绲�(ji茅)妲�(g貌u)锛屽師鏍哥敓鐗╄垏鐪熸牳
鐢熺墿鍩哄洜绲�(ji茅)妲�(g貌u)鐨勫崁(q奴)鍒ワ級锛孯NA 杞�(zhu菐n)閷勭殑鍩烘湰閬庣▼鍜岃綁(zhu菐n)閷勬(j墨)鍣紙灏ゅ叾鏄� RNA 鑱氬悎閰讹級锛�
RNA 杞�(zhu菐n)閷勮垏 DNA 寰�(f霉)鍒堕亷绋嬬殑宸暟锛孯NA 鐨勫壀鎺ュ拰绶ㄨ集锛岃泲鐧借唱(zh矛)鍚堟垚鐨勭敓鐗╁(xu茅)姗�(j墨)鍒�
锛堣泲鐧借唱(zh矛)缈昏鐨勬椹燂紝鐪熸牳鑸囧師鏍哥敓鐗╃炕璀殑鍗€(q奴)鍒ワ級锛岃泲鐧借唱(zh矛)鐨勪慨椋�銆佽綁(zhu菐n)閬�(y霉n)锛堜俊
铏�(h脿o)鑲斤級鍜岄檷瑙ｏ紙铔嬬櫧閰堕珨閫斿緫锛�銆�
3銆佸熀鍥犵殑琛ㄩ仈(d谩)鑸囪(di脿o)鎺�
鍩哄洜琛ㄩ仈(d谩)鐨勬蹇碉紝鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺х殑姘村钩锛屽師鏍哥敓鐗╁熀鍥犺〃閬�(d谩)瑾�(di脿o)鎺х殑鐗归粸(di菐n)锛岃矤(f霉)
瑾�(di脿o)鎺�銆佹瑾�(di脿o)鎺�銆佽獦灏�(d菐o)瑾�(di脿o)鎺у拰闃婚亸瑾�(di脿o)鎺�锛屼钩绯栨搷绺卞瓙鍜岃壊姘ㄩ吀鎿嶇副瀛�锛屜� 鍥犲瓙鐨勮(di脿o)
绡€(ji茅)浣滅敤锛屽師鏍哥敓鐗� mRNA 鐨勭┅(w臎n)瀹氭€�锛孲D 搴忓垪锛涚湡鏍哥敓鐗╁熀鍥犵祫鐨勭祼(ji茅)妲�(g貌u)鐗归粸(di菐n)锛岀湡鏍�
鐢熺墿鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺х殑鐗归粸(di菐n)锛岄爢寮忎綔鐢ㄥ厓浠跺拰鍙嶅紡浣滅敤鍥犲瓙锛岀湡鏍哥敓鐗� DNA 姘村钩涓�
鐨勫熀鍥犺〃閬�(d谩)瑾�(di脿o)鎺�锛孌NA 鐢插熀鍖栬垏鍩哄洜娲绘€х殑瑾�(di脿o)鎺�锛岃泲鐧借唱(zh矛)涔�?锛涚槣?du矛)鍩哄洜琛ㄩ仈(d谩)鐨勫奖
闊�锛岃泲鐧借唱(zh矛)纾烽吀鍖栧皪(du矛)鍩哄洜琛ㄩ仈(d谩)鐨勫奖闊�锛屽弽寮忎綔鐢ㄥ洜瀛愬熀鏈祼(ji茅)妲�(g貌u)锛岀湡鏍稿熀鍥犺綁(zhu菐n)閷勮(di脿o)
鎺х殑涓昏妯″紡锛孯NA 鐨勫姞宸ユ垚鐔�锛宮RNA 绌�(w臎n)瀹氭€ц(di脿o)绡€(ji茅)锛宮RNA 鐨勮瓨(sh铆)鍒ヨ垏铔嬬櫧璩�(zh矛)鍚堟垚锛�
铔嬬櫧璩�(zh矛)淇＞銆�
4銆佸熀鏈垎瀛愮敓鐗╁(xu茅)鎶€琛�(sh霉)
DNA 鍩烘湰鎿嶄綔鎶€琛�(sh霉)锛孯NA 鍩烘湰鎿嶄綔鎶€琛�(sh霉)锛屽熀鍥犲厠闅嗘妧琛�(sh霉)锛屽熀鍥犺〃閬�(d谩)鐮旂┒鎶€琛�(sh霉)锛�
鍩哄洜鏁查櫎鎶€琛�(sh霉)锛岃泲鐧借唱(zh矛)鐩镐簰浣滅敤鐮旂┒鎶€琛�(sh霉)锛岃泲鐧借唱(zh矛)鑸囨牳閰哥浉浜掍綔鐢ㄧ爺绌舵妧琛�(sh霉)锛孲NP
妾㈡脯(c猫)鎶€琛�(sh霉)锛屽熀鍥犺姱鐗囨妧琛�(sh霉)锛岃泲鐧借唱(zh矛)绲勫(xu茅)鐮旂┒鎶€琛�(sh霉)銆�
閲嶉粸(di菐n)鎺屾彙鍒嗗瓙鍏嬮殕鐨勬蹇靛拰閬庣▼锛岀櫦(f膩)鐝�(xi脿n)鍜屽垎闆㈡柊鍩哄洜鐨勬祦绋�銆傚湇绻炲垎瀛愬厠闅�
鐨勬祦绋嬫帉鎻★細RNA 鐨勬彁鍙栬垏绱斿寲锛岃仛鍚堥叾閺堝弽鎳�(y墨ng)锛圥CR 鎶€琛�(sh霉)锛夌殑宸ヤ綔娴佺▼銆佸師鐞�
鍜屾噳(y墨ng)鐢�锛屽熀鍥犳枃搴殑姒傚康銆佺ó椤炲強鍏舵(g貌u)寤烘柟娉�锛屽垎瀛愰洔浜ょ殑瀵�(sh铆)椹�(y脿n)娴佺▼鍜屽垎椤烇紝鍑�
鑶犻浕娉崇殑宸ヤ綔鍘熺悊鍜屾噳(y墨ng)鐢�锛屽熀鍥犲厠闅嗙殑涓昏杓夐珨绯荤当(t菕ng)锛岄檺鍒舵€у収(n猫i)鍒囬叾鐨勯鍒ュ拰鎳�(y墨ng)
鐢ㄧ瓑銆�
浜嗚В鍩哄洜瀹氶粸(di菐n)瑾樿畩锛屾牳閰告脯(c猫)搴忥紝铔嬬櫧璩�(zh矛)绲勫(xu茅)鎶€琛�(sh霉)锛岄叺姣嶉洐闆滀氦绯荤当(t菕ng)锛孌NA 鑺�
鐗囨妧琛�(sh霉)锛屽嚌鑶犻樆婊(sh铆)椹�(y脿n)锛孋hIP 鎶€琛�(sh霉)銆�
5銆佸熀鍥犺(di脿o)鎺ц垏鐢熺墿鐧�(f膩)鑲层€佸厤鐤拰鐤剧梾鐧�(f膩)鐢熺殑闂�(gu膩n)绯�
鐥呮瘨鐧屽熀鍥�锛屽師鐧屽熀鍥狅紝鍘熺檶鍩哄洜鐨勮〃閬�(d谩)瑾�(di脿o)鎺�锛屽熀鍥犳不鐧傦紝鍏嶇柅鐞冭泲鐧藉熀鍥�
绲�(ji茅)妲�(g貌u)锛孖g 鍩哄洜閲嶆帓锛屽厤鐤悆铔嬬櫧鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺�锛屾灉锠呯殑鐧�(f膩)鑲茶垏瑾�(di脿o)鎺�锛岄珮绛夋鐗�
鑺辩櫦(f膩)鑲茬殑鍩哄洜瑾�(di脿o)鎺с€�
涓�銆佸弮鑰冩浉鐩�
1. 鏈辩帀璩㈢瓑绶ㄨ憲锛屻€婄従(xi脿n)浠ｅ垎瀛愮敓鐗╁(xu茅)銆�锛岄珮绛夋暀鑲插嚭鐗堢ぞ锛�2013 骞�锛岀 4 鐗�銆�
2. 娼樺(xu茅)宄扮法钁�锛屻€婄従(xi脿n)浠ｅ垎瀛愮敓鐗╁(xu茅)鏁欑▼銆�锛岀瀛�(xu茅)鍑虹増绀�锛�2009 骞�銆�
3. Robert F. Weaver 钁楋紝銆奙olecular Biology銆�锛孧cGraw-Hill 鍑虹増锛�2011
骞�锛岀 5 鐗堛€�
4. 缇呯磤(j矛)鐩涚瓑鍚堢法锛屻€婄敓鐗╁寲瀛�(xu茅)绨�(ji菐n)鏄庢暀绋嬨€�锛岄珮绛夋暀鑲插嚭鐗堢ぞ锛�2000 骞达紝绗� 3 鐗�銆�
5. M.R.鏍兼灄绛夎憲锛屻€婂垎瀛愬厠闅嗗(sh铆)椹�(y脿n)鎸囧崡銆�锛岀瀛�(xu茅)鍑虹増绀� 2013 骞达紝绗� 4 鐗堛€�
6. 閯敤鐠夌法锛屻€婂熀绀�(ch菙)鍒嗗瓙鐢熺墿瀛�(xu茅)銆嬶紝楂樼瓑鏁欒偛鍑虹増绀�, 2012 骞�锛岀 2 鐗�銆�
鍏嶈铂(z茅)鑱叉槑锛氭湰鏂囩郴杞�(zhu菐n)杓夎嚜缍�(w菐ng)绲�(lu貌)锛屽鏈変镜鐘�锛岃珛(q菒ng)鑱�(li谩n)绯绘垜鍊戠珛鍗冲埅闄�锛屽彟锛氭湰鏂囧儏浠ｈ〃浣滆€呭€�(g猫)浜鸿榛�(di菐n)锛岃垏鏈恫(w菐ng)绔欑劇闂�(gu膩n)銆傚叾鍘熷壍(chu脿ng)鎬т互鍙婃枃涓櫝杩版枃瀛楀拰鍏�(n猫i)瀹规湭缍�(j墨ng)鏈珯璀夊(sh铆)锛屽皪(du矛)鏈枃浠ュ強鍏朵腑鍏ㄩ儴鎴栬€呴儴鍒嗗収(n猫i)瀹广€佹枃瀛楃殑鐪熷(sh铆)鎬�銆佸畬鏁存€�銆佸強鏅�(sh铆)鎬ф湰绔欎笉浣滀换浣曚繚璀夋垨鎵胯锛岃珛(q菒ng)璁€鑰呭儏浣滃弮鑰�锛屽苟璜�(q菒ng)鑷鏍稿(sh铆)鐩搁棞(gu膩n)鍏�(n猫i)瀹�銆�
|
鏂囩珷鎼滅储
鎮ㄧ従(xi脿n)鍦ㄧ殑浣嶇疆锛� 
