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鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗堬紝閮ㄥ垎瀛告牎鏇存柊鑷�2012骞�锛�2013骞�锛涘潎鎻愪緵鏀惰不涓嬭級銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛炴搳鈥�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛炴搳鈥�鑰冨崥瑭﹀嵎搴�鈥� 涓嬭級
鏂扮枂杈叉キ(y猫)澶у鍏ㄦ棩鍒剁ⅸ澹爺绌剁敓鍏ュ鑰冭│
銆婃櫘閫氱敓鐗╁寲瀛搞€嬭€冭│澶х侗
鍛介鏂瑰紡 鎷涚敓鍠綅鑷懡椤� 绉戠洰椤炲垾 鍒濊│
婊垮垎 150
鑰冭│鎬ц唱
鏅€氱敓鐗╁寲瀛歌€冭│鏄偤鎷涙敹杈插椤炵ⅸ澹爺绌剁敓鑰岃ō缃殑閬告嫈鑰冭│銆傚畠鐨勪富瑕佺洰鐨勬槸娓│鑰冪敓灏嶇敓鐗╁寲瀛稿绉戠殑鎶婃彙绋嬪害锛屽寘鎷皪
鐢熺墿鍖栧鍩烘湰姒傚康銆佹柟娉曞拰鎳夌敤鐨勪簡瑙�銆�
鑰冭│鏂瑰紡鍜岃€冭│鏅傞枔
鏅€氱敓鐗╁寲瀛歌€冭│閲囩敤闁夊嵎绛嗚│褰㈠紡锛岃│鍗锋豢鍒嗙偤 150 鍒�锛岃€冭│鏅傞枔鐐� 3 灏忔檪銆�
鑰冭│鍏у鍜岃€冭│瑕佹眰
锛堜竴锛� 闈滄厠(t脿i)鐢熺墿鍖栧
銆愯€冭│鍏у銆�
1.瀹忚浜嗚В锛氶潨鎱�(t脿i)鐢熺墿鍖栧鍒嗘敮涓昏鐐鸿泲鐧借唱鍖栧銆侀叾瀛搞€佺董鐢熺礌鑸囪紨閰�銆佹牳閰稿寲瀛�銆佺硸椤炲寲瀛�銆佽剛椤炶垏鐢熺墿鑶溿€�
2.姒傚康锛氭皑鍩洪吀绛夐浕榛�銆佽泲鐧借唱瀹氱京銆佸鑲姐€佽泲鐧借唱涓€绱氱祼妲�銆佽泲鐧借唱浜岀礆绲愭銆佄�-铻烘棆銆佽泲鐧借唱瓒呬簩绱氱祼妲嬨€佽泲鐧借唱绲愭鍩�銆�
铔嬬櫧璩笁绱氱祼妲嬨€佽泲鐧借唱鍥涚礆绲愭銆佽泲鐧借唱绛夐浕榛炪€佽泲鐧借唱鐨勮畩鎬�銆佽泲鐧借唱鐨勫京鎬с€侀叾鐨勫畾缇�銆佸閰跺京鍚堥珨銆佺背姘忔柟绋�銆佺背姘忓父
鏁�(sh霉) Km 鍊�銆佸彲閫嗘姂鍒朵綔鐢�銆佽畩妲嬮叾銆佸悓宸ラ叾銆佹牳閰�銆侀叾鐨勬椿鍔涘柈浣�銆侀叾娲诲姏銆佹瘮娲诲姏銆佺董鐢熺礌銆佹牳閰稿畾缇┿€佹牳鑻烽吀銆佹牳閰哥殑涓€绱�
绲愭銆佸鑹叉晥鎳�銆佹笡鑹叉晥鎳�銆佹牳閰歌畩鎬�銆佺啍瑙ｆ韩搴� Tm 鍊笺€侀€€鐏�銆佹牳閰稿京鎬�銆佽秴铻烘棆銆佸垎瀛愰洔浜�銆佸柈绯栥€侀洐绯�銆佸绯�銆佷笁閰扮敇
娌�銆佺敇娌圭７鑴�銆佽喗鍥洪唶
3.鐞嗚В鑸囨噳鐢細
锛�1锛夋皑鍩洪吀鐨勫垎椤炲強鍏堕吀鍫挎€х殑鍒ゆ柗;
锛�2锛夋皑鍩洪吀 pK 鍊艰▓绠楀強姘ㄥ熀閰稿湪闆诲牬涓殑娉冲嫊閬风Щ鏂瑰悜;
锛�3锛夋皑鍩洪吀鐨勫寲瀛稿弽鎳夊師鐞嗗強鎳夌敤锛氳寶涓夐叜鍙嶆噳銆�2.4-浜岀鍩鸿嫰鍙嶆噳銆佺暟纭鞍閰搁叝鍙嶆噳銆佷腹纾洪叞姘弽鎳�;
锛�4锛夎泲鐧借唱涓€绱�銆佷簩绱�銆佽秴浜岀礆銆佺祼妲嬪煙鍙婁笁绱�銆佸洓绱氱祼妲嬬殑鐩镐簰闂滅郴鍙婄董鎸佸悇绱氱祼妲嬬殑涓昏浣滅敤鍔�;
锛�5锛夊垎闆㈣泲鐧借唱甯哥敤鏂规硶鍙婂叾鍘熺悊锛�;
锛�6锛夎泲鐧借唱璁婃€х殑鍘熷洜鍙婅畩鎬у悗铔嬬櫧璩祼妲嬬殑璁婂寲;
锛�7锛夎泲鐧借唱浜岀礆绲愭鐨勫熀鏈璞″柈浣嶅強鐗瑰緛;
锛�8锛夐叾鐨勬椿鎬т腑蹇�銆佸簳鐗╃祼鍚堥儴浣�銆佸偓鍖栭儴浣嶅強璁婃閮ㄤ綅涔嬮枔鏄粈涔堥棞绯�;
锛�9锛夐叾鍙嶆噳涓背姘忓父鏁�(sh霉) Km銆侀叾鍙嶆噳閫熷害鐨勮▓绠�;
锛�10锛夊奖闊块叾鍙嶆噳閫熷害鐨勫洜绱犲強褰遍熆鍥犵礌鐨勫師鐞�;
锛�11锛夐叾淇冨弽鎳夊嫊鍔涘涓鐖€ф姂鍒躲€侀潪绔剁埈鎬ф姂鍒�銆佸弽绔剁埈鎬ф姂鍒剁殑涓嶅悓榛炲強 Vmax銆並m 鍊肩殑璁婂寲;
锛�12锛塂NA 闆欒灪鏃嬬祼妲嬪強瓒呰灪鏃嬬祼妲嬬殑鐗归粸;
锛�13锛塂NA 鐔辫畩鎬х殑鐗归粸鍙婄啍瑙ｆ韩搴� Tm 鍊�;
锛�14锛塂NA 鍫垮熀灏嶆暩(sh霉)銆佽灪鏃嬫暩(sh霉)鍙婂牽鍩虹櫨鍒嗘瘮绲勬垚鐨勮▓绠�;
锛�15锛塕NA 鐨勪富瑕侀鍨嬪強姣旇純鍚勯鍨嬬殑涓€绱�銆佷簩绱氬強涓夌礆绲愭鍜屽姛鑳�;
锛�16锛塼RNA 浜岀礆绲愭鐨勭祫鎴愮壒榛炲強鍔熻兘;
锛�17锛夋瘮杓� DNA 鍜� RNA 浜岀礆绲愭鐗归粸;
锛�18锛夌敓鐗╅珨鍏х殑澶氱硸鍒嗗竷鍜岀祫鎴愬舰寮忓強浣滅敤
锛�19锛夌敓鐗╅珨鍏ц剛椤炵殑绋銆佺祫鎴愬拰鍚勮嚜鐨勪綔鐢�
銆愯€冭│瑕佹眰銆�
1锛庣啛鎮夌敓鐗╁寲瀛哥殑鍩烘湰鏍稿績鍏у鍙婂叾瀛哥鐧�(f膩)灞曢€愭鎷撳睍褰㈡垚鐨勫垎鏀�銆�
2. 閫忓竟鐞嗚В闈滄厠(t脿i)鐢熺墿鍖栧涓墍鍖呭惈鐨勬湁闂滄蹇靛拰瀹氱京銆�
3. 鐞嗚В铔嬬櫧璩寲瀛�銆侀叾瀛搞€佹牳閰稿寲瀛镐腑璀樿鍏у鍙婂師鐞�锛屽皪铔嬬櫧璩強鏍搁吀鐨勭祼妲嬨€佺悊鍖栨€ц唱鍙婂姛鑳界浉浜掕伅(li谩n)绯荤悊瑙�锛屽苟鍒嗘瀽鍩烘湰绲�
妲嬫槸濡備綍姹哄畾鐢熺墿瀛稿姛鑳�
锛堜簩锛� 鍕曟厠(t脿i)鐢熺墿鍖栧
銆愯€冭│鍏у銆�
1.瀹忚浜嗚В锛氬嫊鎱�(t脿i)鐢熺墿鍖栧鍒嗘敮涓昏鐐烘柊闄充唬璎濈附璜�銆佺敓鐗╂哀鍖栬垏姘у寲纾烽吀鍖栥€佺硸鐨勫垎瑙ｅ強鍚堟垚浠ｈ瑵銆佽剛椤炵殑鍒嗚В鍙婂悎鎴愪唬璎�銆�
铔嬬櫧璩殑閰朵績璎涜В鍙婃皑鍩洪吀浠ｈ瑵銆佹牳閰哥殑閰朵績闄嶈В鍙婃牳鑻烽吀浠ｈ瑵銆佹牳閰哥殑鐢熺墿鍚堟垚銆佽泲鐧借唱鐨勭敓鐗╁悎鎴�銆佷唬璎濈殑瑾跨瘈(ji茅)
2.姒傚康锛氱敓鐗╂哀鍖栥€佹哀鍖栫７閰稿寲銆佸簳鐗╂按骞崇７閰稿寲銆丳/O 姣�銆侀浕瀛愬偝閬為張锛堝懠鍚搁張锛�銆佺硸閰佃В銆乀CA 涓夌晶閰稿惊鐠�(hu谩n)閫斿緫銆佺７閰告垔绯�
閫斿緫銆乀CA 鍥炶鍙嶆噳銆佺硸鐣扮敓銆佽剛鑲吀鐨� 尾 -姘у寲銆佷箼閱涢吀寰挵(hu谩n)銆侀叜楂�銆佹哀鍖栬劔姘ㄥ熀浣滅敤銆佽綁姘ㄥ熀浣滅敤銆佽伅(li谩n)鍚堣劔姘ㄥ熀浣滅敤銆佸繀闇€姘�
鍩洪吀銆侀檺鍒舵€у収鍒囬叾銆佹牳閰哥殑寰為牠鍚堟垚閫斿緫銆佷腑蹇冩硶鍓�銆佸熀鍥�銆佸崐涓嶉€ｇ簩(x霉)寰╁埗銆佸崐淇濈暀寰╁埗銆佸病宕庣墖娈�銆佽綁閷�銆佸暉鍕曞瓙銆佺祩姝�
瀛�銆侀爢鍙嶅瓙銆侀€嗚綁閷�銆佺炕璀�銆佸収鍚瓙銆佸椤瓙
3.鐞嗚В鑸囨噳鐢細
锛�1锛夌敓鐗╂哀鍖栨寚鐨勬槸浠€涔�銆佺敓鐗╂哀鍖栦腑鐨� CO2銆丠2O 鍜岃兘閲忔槸濡備綍鐢㈢敓;
锛�2锛夐浕瀛愬偝閬為張锛堝懠鍚搁張锛夋寚鐨勬槸浠€涔�銆佸吀鍨嬬殑鍛煎惛閺堟湁鍝簺锛屽畠鍊戝悇鏈変粈涔堢壒榛�;
锛�3锛夐浕瀛愬偝閬為張锛堝懠鍚搁張锛変腑褰㈡垚 ATP 鍙兘鐨勯儴浣嶅強鎶戝埗閮ㄤ綅銆佸強鐩稿皪鎳夌殑鎶戝埗鍔戞槸浠€涔�;
锛�5锛夊懠鍚搁張鏈夊摢浜涚祫鍒嗭紝瀹冨€戝悇鏈変粈涔堢敓鍖栦綔鐢ㄤ互鍙婂湪绶氱矑楂旇啘涓婄殑鎺掑垪闋嗗簭;
锛�4锛夋哀鍖栫７閰稿寲鎸囩殑鏄粈涔�銆佹哀鍖栫７閰稿寲涓� ATP 鏄浣曠敓鎴�锛岀啊杩板叾浣滅敤姗熺悊;
锛�6锛夊寲瀛告徊閫忓亣瑾榛�;
锛�7锛変綍璎傜硸閰佃В锛屽鍑虹硸閰佃В閬庣▼涓叾淇冨弽鎳夋柟绋嬪紡銆佺敘鐗╁強鐢㈢敓鑳介噺鐨勫弽鎳夋椹�锛屽苟瑷堢畻鐢㈢敓鐨� ATP 鏁�(sh霉)閲�;
锛�8锛変綍璎備笁缇ч吀寰挵(hu谩n)锛屽鍑轰笁缇ч吀寰挵(hu谩n)涓叾淇冨弽鎳夋柟绋嬪紡銆佺敘鐗╁強鐢㈢敓鑳介噺鐨勫弽鎳夋椹�锛屽苟瑷堢畻鐢㈢敓鐨� ATP 鏁�(sh霉)閲�;
锛�9锛夎垏绯栫殑绯栭叺瑙�銆佷笁缇ч吀寰挵(hu谩n)鏈夋哀姘у寲涓昏矾鐩告瘮锛岀７閰告垔绯栭€斿緫鏈変綍鐗归粸鍜岀敓鐞嗘剰缇�;
锛�10锛変綍璎傜硸鐣扮敓锛岀啊杩颁笝閰吀鐣扮敓鎴愮硸鐨勯亷绋�;
锛�11锛夌偤浠€涔堜笁缇ч吀寰挵(hu谩n)鏄硸銆佽剛鍜岃泲鐧借唱涓夊ぇ鐗╄唱浠ｈ瑵鐨勫叡鍚岄€氳矾;
锛�12锛夌硸椤炰唬璎濊垏鑴傝偑浠ｈ瑵鏄€氶亷鍝簺鍙嶆噳鑱�(li谩n)绯昏捣渚嗙殑;
锛�13锛夌敓鐗╅珨寰瑰簳姘у寲 1 鍒嗗瓙杌熻剛閰歌兘鐢㈢敓澶氬皯鍒嗗瓙 ATP;
锛�14锛夎剛鑲吀 尾 -姘у寲鏈変綍鐗归粸锛屽湪姝ら亷绋嬩腑鍙冨姞鍙嶆噳鐨勮紨閰舵湁鍝簺锛屽叾鏈€绲傜敘鐗╂槸浠€涔堬紝鍘诲悜濡備綍;
锛�15锛夎剛鑲吀寰為牠鍚堟垚鍜岃剛鑲吀 尾 -姘у寲閬庣▼鍗€(q奴)鍒�锛岀偤浠€涔堣鑴傝偑閰稿緸闋悎鎴愬拰鑴傝偑閰� 尾 -姘у寲涓嶆槸鐩镐簰鐨勯€嗚綁閬庣▼;
锛�16锛夌窗鑳炲収鍙兘鍚堟垚杌熻剛閰�锛屽浜� 16 鍊嬬⒊鍘熷瓙鐨勮剛鑲吀鍦ㄩ珨鍏у浣曠敘鐢�;
锛�17锛夎兘鐩寸殑鎺ョ敓鎴愭父闆㈡皑鍩洪吀鑴皑鍩烘柟寮忔湁鍝簺锛屽悇鏈変綍鐗归粸;
锛�18锛夋皑鍩洪吀鍒嗚В鍚庣敘鐢熺殑姘ㄦ槸濡備綍鎺掑嚭楂斿;
锛�19锛夋瘮杓冨師鏍哥敓鐗� DNA 寰╁埗鑸囪綁閷勭殑涓嶅悓榛�;
锛�20锛夋瘮杓冨師鏍哥敓鐗╁拰鐪熸牳鐢熺墿 DNA 寰╁埗鐨勪笉鍚岄粸
锛�21锛夌啊杩� DNA 鍗婁繚鐣欏京鍒剁殑閬庣▼锛�
锛�22锛夌啊杩板弮鑸� DNA 寰╁埗鐨勯叾鑸囪泲鐧借唱鍥犲瓙锛屼互鍙婂畠鍊戝湪寰╁埗涓殑浣滅敤锛�
锛�23锛夌啊杩� RNA 杞夐寗鐨勯亷绋嬪強鐪熸牳鐢熺墿 mRNA 鐨勬垚鐔熷姞宸ラ亷绋�锛�
锛�24锛変綍璎傚熀鍥犵獊璁婏紝鍩哄洜绐佽畩椤炲瀷鍙婄敓鐗╅珨鐨勪慨寰╂鍒�;
锛�25锛夌啊杩扮敓鐗╅伜鍌充腑蹇冩硶鍓囩殑鍏у;
锛�26锛変粈涔堟槸閬哄偝瀵嗙⒓锛岀啊杩板叾鍩烘湰鐗归粸;
锛�27锛夌啊杩板師鏍哥窗鑳炶泲鐧借唱鐨勫悎鎴愰亷绋�;
锛�28锛変綍璎傚熀鍥犲伐绋嬶紝绨¤堪 PCR 鍙嶆噳鍘熺悊鍙婇亷绋�;
锛�29锛夌敓鐗╅珨鍏х硸銆佽剛鍙婅泲鐧借唱涓夐鐗╄唱鍦ㄤ唬璎濅笂鐨勭浉浜掗棞绯诲浣�;
锛�30锛夌啊杩扮窗鑳炴按骞宠绡€(ji茅)鐨勪富瑕佹柟寮�;
锛�31锛夌啊杩颁钩绯栨搷绺卞瓙鐨勬璨犺鎺ф鍒�;
銆愯€冭│瑕佹眰銆�
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