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鍏ョ殑浜嗚В锛岄噸榛炴帉鎻″钩琛＄殑鍘熺悊銆佹憾娑蹭腑鐨勫悇绋寲瀛�(xu茅)骞宠 鍙�
鍏跺湪鍒嗘瀽鍖栧(xu茅)涓殑鎳�(y墨ng)鐢�锛屽缓绔嬫簴(zh菙n)纰虹殑鈥滈噺鈥濈殑姒傚康鍜屾帉鎻″悇绋�
鍖栧(xu茅)鍒嗘瀽鏂规硶锛涙帉鎻″寲瀛�(xu茅)鍙嶆噳(y墨ng)閫熺巼銆佺墿璩�(zh矛)绲�(ji茅)妲�(g貌u)銆佸垎鏁ｉ珨绯荤瓑
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閲嬪寲瀛�(xu茅)鐝�(xi脿n)璞�锛屾渻閬嬬敤鍩烘湰鍒嗘瀽鏂规硶鍜屾脯瑭︽墜娈甸€�(j矛n)琛屼竴鑸殑鍖�
瀛�(xu茅)鍒嗘瀽锛岃兘澶犻亱鐢ㄦ墍瀛�(xu茅)鐭ヨ瓨瑙ｆ焙鐢熺敘(ch菐n)鐢熸椿涓殑瀵﹂殯鍟忛锛岃兘
灏囧寲瀛�(xu茅)鐭ヨ瓨鑸囧皥妤�(y猫)瀵﹂殯鐩哥祼(ji茅)鍚�銆�
涓€銆佽€冭│鍏�(n猫i)瀹�
1銆佹埃楂斿強鍖栧(xu茅)鐔卞寲瀛�(xu茅)鍒濇
(1) 姘ｉ珨锛氱悊鎯虫埃楂旂媭鎱�(t脿i)鏂圭▼锛岄亾鐖鹃爴鍒嗗瀹氬緥
(2) 鐔卞姏瀛�(xu茅)绗竴瀹氬緥锛氱挵(hu谩n)澧冭垏楂旂郴銆佺媭鎱�(t脿i)鑸囩媭鎱�(t脿i)鍑芥暩(sh霉)銆佸収(n猫i)
鑳�銆佸姛鍜岀啽
(3) 鍙嶆噳(y墨ng)鐔卞拰鑷敱鑳借畩鐨勫惈缇╁強鍏惰▓绠�锛涜嚜鐧�(f膩)鎬у垽鎿�(j霉)锛涜搵
鏂畾寰�
2銆佸寲瀛�(xu茅)骞宠 鍜屽寲瀛�(xu茅)鍙嶆噳(y墨ng)閫熺巼
(1) 鍙嶆噳(y墨ng)閫熺巼鐨勮〃绀烘柟娉�锛涜唱(zh矛)閲忎綔鐢ㄥ畾寰�锛涢樋绱凹鐑忔柉鍏�
寮�
(2) 妯�(bi膩o)婧�(zh菙n)骞宠 甯告暩(sh霉)锛涘寲瀛�(xu茅)骞宠 鐨勮▓绠�锛涘寲瀛�(xu茅)鍙嶆噳(y墨ng)绛夋韩鏂圭▼
鐨勬噳(y墨ng)鐢�
(3) 婧舵恫閰稿害瑷堢畻锛涚珐娌栨憾娑�锛涘垎姝ユ矇娣€锛涢浕妤甸浕鍕�锛涘父
鐢ㄦ淮瀹氭柟娉�
璩�(zh矛)瀛愬钩琛″紡锛涙憾娑茬殑 pH 鍊煎強鍏惰▓绠楋紱绶╂矕浣滅敤
鍘熺悊锛涙哀鍖栭倓鍘熷弽鎳�(y墨ng)鏂圭▼寮忕殑閰嶅钩锛涘師闆绘睜锛岄浕妤�
闆诲嫝锛屾(bi膩o)婧�(zh菙n)闆绘サ闆诲嫝锛屽師闆绘睜闆诲嫊鍕㈢殑瑷堢畻锛岃▓绠�
鍘熼浕姹犻浕鍕曞嫝锛涘垽鏂峰弽鎳�(y墨ng)鏂瑰悜锛涜▓绠楀钩琛″父鏁�(sh霉)銆並sp
鍙婃憾娑� pH锛汦DTA 鐨勬€ц唱(zh矛)鍙婂湪婧舵恫涓殑瑙ｉ洟骞宠 锛�
閲戝爆鎸囩ず鍔戠殑璁婅壊鍘熺悊锛涘父鐢ㄩ噾灞寚绀哄姂锛�
(4) 婊村畾娉曪細婊村畾鏇茬窔鑸囨寚绀哄姂浠ュ強婊村畾鐨勬噳(y墨ng)鐢�
3銆佸師瀛愮祼(ji茅)妲�(g貌u)鍜屽厓绱犲懆鏈熷緥
(1) 鍘熷瓙鏍稿闆诲瓙鐨勯亱鍕曠媭鎱�(t脿i)锛氱幓鐖剧殑鍘熷瓙绲�(ji茅)妲�(g貌u)鐞嗚珫鍜岄浕
瀛愮殑鐜荤矑浜岃薄鎬�
(2) 娉㈠嚱鏁�(sh霉)銆佹鐜囧瘑搴�銆侀浕瀛愪簯锛涘洓鍊嬮噺瀛愭暩(sh霉)
(3) 鍘熷瓙鏍稿闆诲瓙鎺掑竷鑸囧厓绱犲懆鏈熷緥
4銆佸寲瀛�(xu茅)閸佃垏鐗╄唱(zh矛)绲�(ji茅)妲�(g貌u)
(1) 闆㈠瓙閸点€佸叡鍍归嵉鐞嗚珫
(2) 闆滃寲杌岄亾鐞嗚珫瑕侀粸銆侀洔鍖栬粚閬撻鍨嬭垏鍒嗗瓙骞句綍妲�(g貌u)鍨�
(3) 浜嗚В鍒嗗瓙闁撳姏鍜屾矮閸�
5銆佸寲瀛�(xu茅)鍒嗘瀽
(1) 瑾ゅ樊鐨勫垎椤�銆佷締婧愩€佹笡鍏嶆柟娉�锛屾簴(zh菙n)纰哄害銆佺簿瀵嗗害鐨勬
蹇靛強鍏惰〃绀烘柟娉�
(2) 鏈夋晥鏁�(sh霉)瀛楀湪鍒嗘瀽瀵﹁笎涓殑閬嬬敤
(3) 閲嶉噺鍒嗘瀽锛涙淮瀹氬垎鏋�
(4) 妯�(bi膩o)婧�(zh菙n)婧舵恫鐨勮〃绀烘柟娉曞強閰嶅埗銆佹(bi膩o)瀹氭柟娉曪紱婊村畾鍒嗘瀽瑷�
绠�
(5) 鍏夊害鍒嗘瀽娉�
鏈椾集姣旂埦瀹氬緥锛涘惛鍏夌郴鏁�(sh霉)锛涘柈涓€绲勫垎鐨勫垎鏋�锛涘绲�
鍒嗗垎鏋�銆�
6銆佸垎鏋愬寲瀛�(xu茅)涓父鐢ㄧ殑鍒嗛鏂规硶鍜岀敓鐗╄│妯ｇ殑鍓嶈檿鐞�
(1)鍒嗘瀽鍖栧(xu茅)涓父鐢ㄧ殑鍒嗛洟鏂规硶
(2)鐢熺墿瑭︽ǎ鐨勫墠铏曠悊
浜�銆佽€冭│瑕佹眰(瑕佹眰鎺屾彙鍜屼簡瑙ｇ殑鍚勭珷鍏�(n猫i)瀹�)
绗竴绔� 姘ｉ珨鍜屾憾娑�
鎺屾彙鐞嗘兂姘ｉ珨鐙€鎱�(t脿i)鏂圭▼寮忓強鍏舵噳(y墨ng)鐢�銆�
鎺屾彙閬撶埦闋撳垎澹撳畾寰�銆�
鎺屾彙绋€婧舵恫鐨勪緷鏁�(sh霉)鎬у強鍏舵噳(y墨ng)鐢�銆�
鐔熸倝鑶犻珨鐨勭祼(ji茅)妲�(g貌u)銆佹€ц唱(zh矛)銆佺┅(w臎n)瀹氭€у強鑱氭矇浣滅敤銆�
1.1 姘ｉ珨
鎺屾彙鐞嗘兂姘ｉ珨鐙€鎱�(t脿i)鏂圭▼寮忓強閬撶埦闋撳垎澹撳畾寰�
1.2 婧舵恫
浜嗚В鍒嗘暎绯诲強绋€婧舵恫鐨勯€氭€�
1.3 鑶犻珨婧舵恫
浜嗚В婧惰啝鐨勫埗鍌�锛屾憾鑶犵殑鎬ц唱(zh矛)锛岃啝鍦�(tu谩n)绲�(ji茅)妲�(g貌u)鍜岄浕鍕曢浕鍕�锛�
婧惰啝鐨勭┅(w臎n)瀹氭€ц垏鑱氭矇
绗簩绔� 鍖栧(xu茅)鐔卞姏瀛�(xu茅)鍒濇
2.1 浜嗚В鐔卞姏瀛�(xu茅)鑳�銆佺創銆佺喌銆佽嚜鐢辫兘绛夌媭鎱�(t脿i)鍑芥暩(sh霉)鐨勬蹇�
2.2 鎺屾彙鐔卞姏瀛�(xu茅)绗竴瀹氬緥锛岀浜屽畾寰嬬殑鍩虹(ch菙)鍏�(n猫i)瀹�
2.3 鎺屾彙鍖栧(xu茅)鍙嶆噳(y墨ng)鐔辨晥鎳�(y墨ng)鐨勫悇绋▓绠楁柟娉�
2.4 鎺屾彙閬庣▼鐨勨柍S銆佲柍G 鐨勮▓绠�
2.5 鎺屾彙鈻矴 鑸囨韩搴︾殑闂�(gu膩n)绯诲紡锛屽強婧害灏嶅弽鎳�(y墨ng)鑷櫦(f膩)鎬х殑褰�
闊�
绗笁绔� 鍖栧(xu茅)骞宠
浜嗚В缍�(j墨ng)椹楀钩琛″父鏁�(sh霉)鑸囨(bi膩o)婧�(zh菙n)骞宠 甯告暩(sh霉)浠ュ強妯�(bi膩o)婧�(zh菙n)骞宠 甯告暩(sh霉)鑸�
妯�(bi膩o)婧�(zh菙n)鍚夊竷鏂嚜鐢辫兘璁婄殑闂�(gu膩n)绯�銆�
鎺屾彙涓嶅悓鍙嶆噳(y墨ng)椤炲瀷鐨勬(bi膩o)婧�(zh菙n)骞宠 甯告暩(sh霉)琛ㄩ仈(d谩)寮�锛屽苟鑳藉緸瑭茶〃
閬�(d谩)寮忕悊瑙ｅ寲瀛�(xu茅)骞宠 鐨勭Щ鍕�銆�
鎺屾彙鏈夐棞(gu膩n)鍖栧(xu茅)骞宠 鐨勮▓绠�锛屽寘鎷亱鐢ㄥ閲嶅钩琛¤(gu墨)鍓囬€�(j矛n)琛�
瑷堢畻銆�
鎺屾彙鍖栧(xu茅)骞宠 绉诲嫊鐨勫畾鎬у垽鏂蜂互鍙婄Щ鍕曠▼搴︾殑瀹氶噺瑷�
绠�銆�
3.1 姝ｇ⒑鐞嗚В骞宠 甯告暩(sh霉)鐨勭墿鐞嗘剰缇╁強琛ㄧず鏂规硶
3.2 鎺屾彙 Gibbs 鑷敱鑳借畩鑸囧钩琛″父鏁�(sh霉)鐨勯棞(gu膩n)绯�锛屽苟鑳界啛绶村湴
閫�(j矛n)琛屾湁闂�(gu膩n)骞宠 甯告暩(sh霉)鐨勮▓绠�
3.3 鍒╃敤 Van鈥檛 Hoff 绛夋韩寮忓垽鏂蜂换鎰忕郸瀹氭浠朵笅鍖栧(xu茅)鍙嶆噳(y墨ng)
鐨勬柟鍚�
3.4 閬嬬敤骞宠 绉诲嫊鍘熺悊瑾槑婧害銆佹績搴﹀鍔涘皪鍖栧(xu茅)骞宠
绉诲嫊鐨勫奖闊�
3.5 澶氶噸骞宠 瑕�(gu墨)鍓�
绗洓绔� 鍖栧(xu茅)鍙嶆噳(y墨ng)閫熺巼
浜嗚В鍖栧(xu茅)鍙嶆噳(y墨ng)閫熺巼鐨勬蹇靛強鍏跺椹楁脯瀹氭柟娉�銆�
鎺屾彙璩�(zh矛)閲忎綔鐢ㄥ畾寰嬪拰鍙嶆噳(y墨ng)鐨勯€熺巼鏂圭▼寮�銆�
鎺屾彙闃跨疮灏肩儚鏂稉(j墨ng)椹楀紡锛屽苟鑳界敤娲诲寲鍒嗗瓙銆佹椿鍖栬兘绛夋
蹇佃В閲嬪悇绋鐣屽洜绱犲皪鍙嶆噳(y墨ng)閫熺巼鐨勫奖闊�銆�
4.1 鍖栧(xu茅)鍙嶆噳(y墨ng)閫熺巼鍙婂叾琛ㄧず娉�
4.2 婵冨害灏嶅弽鎳�(y墨ng)閫熺巼鐨勫奖闊�
4.2.1 鍩哄厓鍙嶆噳(y墨ng)鑸囬潪鍩哄厓鍙嶆噳(y墨ng)
4.2.2 璩�(zh矛)閲忎綔鐢ㄥ畾寰�
4.2.3 闈炲熀鍏冨弽鎳�(y墨ng)閫熺巼鏂圭▼寮忕殑纰哄畾
4.2.4 鍙嶆噳(y墨ng)姗�(j墨)鐞�
4.3 婧害灏嶅弽鎳�(y墨ng)閫熺巼鐨勫奖闊�
4.4 鍙嶆噳(y墨ng)閫熺巼鐞嗚珫绨′粙
4.4.1 纰版挒鐞嗚珫
4.4.2 閬庢浮鎱�(t脿i)鐞嗚珫
4.5 鍌寲鍔戝皪鍙嶆噳(y墨ng)閫熺巼鐨勫奖闊�
绗簲绔� 瑙ｉ洟骞宠
浜嗚В娲诲害銆侀洟瀛愬挤(qi谩ng)搴︾瓑姒傚康銆�
鐞嗚В绶╂矕浣滅敤鍘熺悊浠ュ強绶╂矕婧舵恫鐨勭祫鎴愬拰鎬ц唱(zh矛)锛屾帉鎻＄珐
娌栨憾娑� pH 鍊艰▓绠�銆�
鐞嗚В闆ｆ憾闆昏В璩�(zh矛)娌夋穩婧惰В骞宠 鐨勭壒榛�锛屾渻閬嬬敤婧跺害绌嶈(gu墨)
鍓囧垽鏂锋矇娣€婧惰В骞宠 鐨勭Щ鍕曚互鍙婃湁闂�(gu膩n)瑷堢畻銆�
5.1 閰稿牽鐞嗚珫
5.1.1 閰稿牽璩�(zh矛)瀛愮悊璜�
5.1.2 閰稿牽闆诲瓙鐞嗚珫
5.2 寮遍吀銆佸急鍫跨殑瑙ｉ洟骞宠
5.2.1 涓€鍏冨急閰搞€佸急鍫跨殑瑙ｉ洟骞宠
5.2.2 澶氬厓寮遍吀銆佸急鍫跨殑瑙ｉ洟骞宠
5.2.3 鍏╂€х墿璩�(zh矛)鐨勮В闆㈠钩琛�
5.2.4 鍚岄洟瀛愭晥鎳�(y墨ng)鍜岄菇鏁堟噳(y墨ng)
5.3 寮�(qi谩ng)闆昏В璩�(zh矛)婧舵恫
5.3.1 闆㈠瓙姘涙蹇�
5.3.2 娲诲害鍜屾椿搴︾郴鏁�(sh霉)
5.4 绶╂矕婧舵恫
5.4.1 绶╂矕浣滅敤鍘熺悊鍜岃▓绠楀叕寮�
5.4.2 绶╂矕瀹归噺鍜岀珐娌栬寖鍦�
5.5 娌夋穩婧惰В骞宠
5.5.1 婧跺害绌嶅拰婧跺害绌嶈(gu墨)鍓�
5.5.2 娌夋穩鐨勭敓鎴愬拰婧惰В
5.5.3 鍒嗘娌夋穩鍜屾矇娣€鐨勮綁(zhu菐n)鍖�
绗叚绔� 姘у寲閭勫師鍙嶆噳(y墨ng)
鎺屾彙姘у寲閭勫師鍙嶆噳(y墨ng)鐨勫熀鏈蹇碉紝鑳介厤骞虫哀鍖栭倓鍘熷弽鎳�(y墨ng)
寮�銆�
鐞嗚В闆绘サ闆诲嫝鐨勬蹇�锛岃兘鐢ㄨ兘鏂壒鍏紡閫�(j矛n)琛屾湁闂�(gu膩n)瑷堢畻銆�
鎺屾彙闆绘サ闆诲嫝鍦ㄦ湁闂�(gu膩n)鏂归潰鐨勬噳(y墨ng)鐢ㄣ€�
浜嗚В鍘熼浕姹犻浕鍕曞嫝鑸囧悏甯冩柉鑷敱鑳借畩鐨勯棞(gu膩n)绯�銆�
鎺屾彙鍏冪礌闆诲嫝鍦栧強鍏舵噳(y墨ng)鐢�銆�
6.1 姘у寲閭勫師鍙嶆噳(y墨ng)鐨勫熀鏈蹇�
6.2 姘у寲閭勫師鏂圭▼寮忛厤骞�
6.3 闆绘サ闆诲嫝
鎺屾彙鍘熼浕姹狅紝闆绘サ闆诲嫝锛岃兘鏂壒鏂圭▼寮�锛屽師闆绘睜鐨勯浕鍕�
鍕㈣垏鈻硆G 鐨勯棞(gu膩n)绯�
6.4 闆绘サ闆诲嫝鐨勬噳(y墨ng)鐢�
瑷堢畻鍘熼浕姹犵殑闆诲嫊鍕�锛屽垽鏂锋哀鍖栭倓鍘熷弽鎳�(y墨ng)閫�(j矛n)琛岀殑鏂瑰悜锛�
閬告搰姘у寲鍔戝拰閭勫師鍔�锛屽垽鏂锋哀鍖栭倓鍘熷弽鎳�(y墨ng)閫�(j矛n)琛岀殑娆″簭锛屾脯瀹�
鏌愪簺鍖栧(xu茅)甯告暩(sh霉)
6.5 鍏冪礌闆诲嫝鍦栧強鍏舵噳(y墨ng)鐢�
绗竷绔� 鍘熷瓙绲�(ji茅)妲�(g貌u)
浜嗚В鏍稿闆诲瓙閬嬪嫊鐨勭壒娈婃€р€旀尝绮掍簩璞℃€�銆�
鑳界悊瑙ｆ尝鍑芥暩(sh霉)瑙掑害鍒嗗竷鍦�锛岄浕瀛愪簯瑙掑害鍒嗗竷鍦栧拰闆诲瓙浜�
寰戝悜鍒嗗竷鍦�銆�
鎺屾彙鍥涘€嬮噺瀛愭暩(sh霉)鍙婄墿鐞嗘剰缇�锛屾帉鎻￠浕瀛愬堡銆侀浕瀛愪簽灞�銆�
鑳界礆銆佽粚閬撶瓑鍚京銆�
鑳界敤涓嶇浉瀹瑰師鐞�銆佽兘閲忔渶浣庡師鐞嗐€佹椽鐗硅(gu墨)鍓囧鍑轰竴鑸厓
绱犵殑鍘熷瓙鏍稿闆诲瓙鎺掑竷寮忓拰鍍归浕瀛愭(g貌u)鍨�銆�
7.1 寰绮掑瓙鐨勬尝绮掍簩璞℃€�
7.1.1 姘厜璀滃拰鐜荤埦鐞嗚珫
7.1.2 寰绮掑瓙鐨勬尝绮掍簩璞℃€�
7.2 姘師瀛愭牳澶栭浕瀛愮殑閬嬪嫊鐙€鎱�(t脿i)
7.2.1 娉㈠嚱鏁�(sh霉)鍜岃枦瀹氳鏂圭▼
7.2.2 娉㈠嚱鏁�(sh霉)鍜岄浕瀛愪簯鍦栧舰
7.2.3 鍥涘€嬮噺瀛愭暩(sh霉)
7.3 澶氶浕瀛愬師瀛愭牳澶栭浕瀛愮殑閬嬪嫊鐙€鎱�(t脿i)
灞忚斀鏁堟噳(y墨ng)鍜岄墕绌挎晥鎳�(y墨ng),鍘熷瓙鏍稿闆诲瓙鎺掑竷
7.4 鍘熷瓙绲�(ji茅)妲�(g貌u)鍜屽厓绱犲懆鏈熷緥
7.4.1 鏍稿闆诲瓙鎺掑竷鍜屽懆鏈熻〃鐨勯棞(gu膩n)绯�
7.4.2 鍘熷瓙绲�(ji茅)妲�(g貌u)鑸囧厓绱犲熀鏈€ц唱(zh矛)
绗叓绔� 鍒嗗瓙绲�(ji茅)妲�(g貌u)
鎺屾彙闆㈠瓙閸电悊璜栬榛烇紝鐞嗚В姹哄畾闆㈠瓙鍖栧悎鐗╂€ц唱(zh矛)鐨勫洜绱�
鍙婇洟瀛愬寲鍚堢墿鐨勭壒寰�銆�
鎺屾彙闆诲瓙閰嶅皪娉曞強鍏卞児閸电殑鐗瑰緛銆�
鑳界敤杌岄亾闆滃寲鐞嗚珫渚嗚В閲嬩竴鑸垎瀛愮殑妲�(g貌u)鍨嬨€�
浜嗚В闆㈠瓙妤靛寲銆佸垎瀛愰枔鍔涚殑姒傚康锛屾帉鎻℃矮閸电殑褰㈡垚鍜岀壒
寰併€�
8.1 闆㈠瓙閸靛拰鍏卞児閸�
鎺屾彙鍍归嵉鐞嗚珫锛屽叡鍍归嵉鐨勭壒寰�
8.2 杌岄亾闆滃寲鐞嗚珫
浜嗚В杌岄亾闆滃寲鐞嗚珫鐨勫熀鏈榛烇紝鎺屾彙闆滃寲杌岄亾鐨勯鍨�
8.3 鍍瑰堡闆诲瓙灏嶄簰鏂ョ悊璜�
8.4 鍒嗗瓙杌岄亾鐞嗚珫绨′粙
8.5 鍒嗗瓙鐨勬サ鎬у拰鍒嗗瓙闁撳姏
8.6 姘嵉
绗節绔� 閰嶄綅鍖栧悎鐗�
鎺屾彙閰嶄綅鍖栧悎鐗╃殑绲勬垚銆佸畾缇�銆侀鍨嬪拰绲�(ji茅)妲�(g貌u)鐗归粸銆�
鐞嗚В閰嶄綅闆㈣В骞宠 鐨勬剰缇╁強鏈夐棞(gu膩n)瑷堢畻銆�
鎺屾彙铻悎鐗╃殑鐗归粸鍙婃噳(y墨ng)鐢�銆�
9.1 閰嶄綅鍖栧悎鐗╃殑绲勬垚鍜屽畾缇�
9.2 閰嶄綅鍖栧悎鐗╃殑椤炲瀷鍜屽懡鍚�
9.3 閰嶄綅瑙ｉ洟骞宠
鎺屾彙閰嶄綅瑙ｉ洟骞宠 鍜屽钩琛″父鏁�(sh霉)锛岄厤浣嶈В闆㈠钩琛＄殑绉诲嫊锛�
鎺屾彙 EDTA 婊村畾娉曠殑鍩烘湰鍘熺悊锛�
9.4 铻悎鐗╃殑绌�(w臎n)瀹氭€�
鎺屾彙铻悎鐗╃殑绲�(ji茅)妲�(g貌u)鐗归粸鍙婄┅(w臎n)瀹氭€�锛屼簡瑙ｈ灟鍚堝姂鐨勬噳(y墨ng)鐢�
绗崄绔� 瀹氶噺鍒嗘瀽鐨勮宸拰鍒嗘瀽绲�(ji茅)鏋滅殑鏁�(sh霉)鎿�(j霉)铏曠悊
鐞嗚В鏈夋晥鏁�(sh霉)瀛楃殑鎰忕京锛屾帉鎻″畠鐨勯亱绠楄(gu墨)鍓�銆�
浜嗚В瀹氶噺鍒嗘瀽瑾ゅ樊鐨勭敘(ch菐n)鐢熷拰瀹冪殑鍚勭ó琛ㄧず鏂规硶銆�
浜嗚В鎻愰珮鍒嗘瀽绲�(ji茅)鏋滄簴(zh菙n)纰哄害鐨勬柟娉�銆�
鎺屾彙鍒嗘瀽绲�(ji茅)鏋滄湁闄愬椹楁暩(sh霉)鎿�(j霉)鐨勮檿鐞嗘柟娉曘€�
10.1 鏈夋晥鏁�(sh霉)瀛�
10.2 瑾ゅ樊鐨勭敘(ch菐n)鐢熷強琛ㄧず鏂规硶
浜嗚В绲曞皪瑾ゅ樊鍜岀浉灏嶈宸�锛岀郴绲�(t菕ng)瑾ゅ樊鍜岄毃姗�(j墨)瑾ゅ樊锛�
鎺屾彙婧�(zh菙n)纰哄害鍜岀簿瀵嗗害
10.3 鏈夐檺瀵﹂鏁�(sh霉)鎿�(j霉)鐨勭当(t菕ng)瑷堣檿鐞�
鎺屾彙娓畾绲�(ji茅)鏋滈洟缇ゅ€肩殑妫勮垗锛岄’钁楁€ф椹�锛屽垎鏋愮祼(ji茅)
鏋滅殑鏁�(sh霉)鎿�(j霉)铏曠悊鑸囧牨鍛�
10.4 鎻愰珮鍒嗘瀽绲�(ji茅)鏋滄簴(zh菙n)纰哄害鐨勬柟娉�
閬告搰鍚堥仼鐨勫垎鏋愭柟娉�锛屽浣曟笡灏忔脯閲忕殑鐩稿皪瑾ゅ樊銆�
绯荤当(t菕ng)瑾ゅ樊鍜岄毃姗�(j墨)瑾ゅ樊
绗崄涓€绔� 閲嶉噺鍒嗘瀽娉�
浜嗚В閲嶉噺鍒嗘瀽娉曠殑鍩烘湰鍘熺悊鍜屼富瑕佹椹熴€�
绨¤浜嗚В娌夋穩鐨勫舰鎴愰亷绋�锛屾脯瀹氭浠剁殑閬告搰銆�
鎺屾彙閲嶉噺鍒嗘瀽绲�(ji茅)鏋滆▓绠楁柟娉曘€�
11.1 閲嶉噺鍒嗘瀽娉曟杩�
11.2 娌夋穩鐨勫畬鍏ㄧ▼搴﹁垏褰遍熆娌夋穩婧惰В搴︾殑鍥犵礌
浜嗚В娌夋穩婧惰В搴︾殑澶у皬锛屾槸姹哄畾娌夋穩鏄惁瀹屽叏鐨勪富
瑕佸洜绱�
11.3 褰遍熆娌夋穩绱斿害鐨勫洜绱�
鍏辨矇娣€鍜屽悗娌夋穩銆佽〃闈㈠惛闄�銆佺敓鎴愭贩鏅�銆佸寘钘忕瓑鏄�
寮曡捣鍏辨矇娣€鐨勮涓诲師鍥�銆�
11.4 娌夋穩鐨勫舰鎴愯垏娌夋穩姊濅欢
11.5 娌夋穩鐨勯亷婵俱€佹礂婊�銆佺儤骞叉垨鐏肩噿鍜屽垎鏋愮祼(ji茅)鏋滅殑瑷堢畻
绗崄浜岀珷 婊村畾鍒嗘瀽娉�
浜嗚В妯�(bi膩o)婧�(zh菙n)婧舵恫鐨勯厤鍒舵柟娉曪紝鎺屾彙鍩烘簴(zh菙n)鐗╄唱(zh矛)鎳�(y墨ng)鍏峰倷鐨勬
浠�锛涙淮瀹氬垎鏋愭硶鐨勫熀鏈煡璀�銆�
鐔熺反鎺屾彙閰嶄綅婊村畾涓壇鍙嶆噳(y墨ng)绯绘暩(sh霉)鐨勮▓绠�
鐔熸倝甯哥敤閲戝爆鎸囩ず鍔戝強 EDTA 娉曠殑鎳�(y墨ng)鐢ㄨ寖鍦�
鐔熸倝閰稿牽鎸囩ず鍔戠殑璁婅壊鍘熺悊鍙婂叾閬告搰
鎺屾彙閰稿牽婧舵恫涓矮闆㈠瓙婵冨害鐨勮▓绠�锛屾按婧舵恫涓吀鍫挎淮
瀹氭硶鐨勫師鐞�
鎺屾彙閰稿牽婊村畾銆侀厤浣嶆淮瀹�銆佹哀鍖栭倓鍘熸淮瀹氬拰娌夋穩婊村畾
娉曠殑鍩烘湰鍘熺悊銆�
鐔熸倝鍚勭ó婊村畾鍒嗘瀽娉曠殑瀵﹂殯鎳�(y墨ng)鐢ㄣ€�
12.1 婊村畾鍒嗘瀽娉曟璜�
浜嗚В婊村畾鍒嗘瀽閬庣▼鍜屾柟娉曞垎椤�锛屾淮瀹氬垎鏋愭硶灏嶅寲瀛�(xu茅)鍙嶆噳(y墨ng)
鐨勮姹傚拰婊村畾鏂瑰紡銆傛帉鎻℃(bi膩o)婧�(zh菙n)婧舵恫鐨勯厤鍒�銆佸熀婧�(zh菙n)鐗┿€佸熀
婧�(zh菙n)婧舵恫鍙婃湁闂�(gu膩n)瑷堢畻
12.2 閰稿牽婊村畾娉�
12.2.1 寮遍吀鍫挎憾娑蹭腑鍚勭墿绋殑鍒嗗竷
12.2.2 閰稿牽婧舵恫姘洟瀛愭績搴︾殑瑷堢畻
12.2.3 绶╂矕婧舵恫
12.2.4 閰稿牽鎸囩ず鍔�
12.2.5 婊村畾鏇茬窔鍙婃寚绀哄姂鐨勯伕鎿�
12.2.6 閰稿牽婊村畾娉曠殑鎳�(y墨ng)鐢�
12.3 閰嶄綅婊村畾娉�
12.3.1 閰嶄綅婊村畾娉曟杩�
12.3.2 姘ㄧ晶閰嶄綅鍔戣垏閰嶄綅骞宠
12.3.3 閰嶄綅婊村畾鐨勫熀鏈師鐞�
12.3.4 娣峰悎闆㈠瓙鐨勬淮瀹�
12.3.5 閰嶄綅婊村畾鐨勬柟寮忓拰鎳�(y墨ng)鐢ㄧず渚�
12.4 姘у寲鍘熼倓婊村畾娉�
12.4.1 姘у寲閭勫師婊村畾娉曟杩�
12.4.2 姘у寲閭勫師婊村畾娉曞熀鏈師鐞�
12.4.3 姘у寲閭勫師婊村畾娉曞垎椤炲強鎳�(y墨ng)鐢�
12.5 娌夋穩婊村畾娉�
鎺屾彙婊村畾鏇茬窔锛岀祩榛炴娓強鍏舵噳(y墨ng)鐢�
绗崄涓夌珷 姣旇壊娉曞拰鍒嗗厜鍏夊害娉�
浜嗚В姣旇壊娉曞拰鍒嗗厜鍏夊害娉曠殑鐗归粸銆�
鎺屾彙鍏夌殑鍚告敹瀹氬緥鍙婂叾閬╃敤鑼冨湇銆�
鎺屾彙鍒嗗厜鍏夊害娉曠殑鍒嗘瀽鏂规硶銆�
浜嗚В椤壊鍙嶆噳(y墨ng)鍙婂叾姊濅欢鐨勯伕鎿�銆�
浜嗚В鍒嗗厜鍏夊害娉曠殑鏌愪簺鎳�(y墨ng)鐢�銆�
13.1 姒傝堪
浜嗚В鍏夊害鍒嗘瀽娉曠殑鐗归粸鍙婂叾鐗╄唱(zh矛)灏嶅厜鐨勯伕鎿囨€у惛鏀�
13.2 鍏夊惛鏀剁殑鍩烘湰瀹氬緥
鎺屾彙鏈椾集锛嶆瘮鐖惧畾寰嬪強鍏跺惛鍏夊害鐨勫姞鍜屾€�
13.3 姣旇壊娉曞拰鍒嗗厜鍏夊害娉曞強鍏跺剙鍣�
浜嗚В鐩姣旇壊娉�銆佸垎鍏夊厜搴﹁▓鐨勫熀鏈儴浠跺強甯哥敤鐨勫咕绋垎
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