|
鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗堬紝閮ㄥ垎瀛告牎鏇存柊鑷�2012骞�锛�2013骞�锛涘潎鎻愪緵鏀惰不涓嬭級銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛炴搳鈥�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛炴搳鈥�鑰冨崥瑭﹀嵎搴�鈥� 涓嬭級
642-鐢熺墿鍖栧锛堥啱(y墨)锛�
涓€銆佽€冭│鐩殑
鐢熺墿鍖栧鏄敓鐗╁寲瀛歌垏鍒嗗瓙鐢熺墿瀛稿皥妤�(y猫)鍙婂叾瀹冪浉闂滃皥妤�(y猫)纰╁＋鐮旂┒鐢熺殑鍏ュ鑰冭│绉戠洰涔�
涓€銆傜敓鐗╁寲瀛哥爺绌剁敓鍏ュ鑰冭│鏄偤鎵€鎷涙敹鑸囩敓鐗╁寲瀛告湁闂滃皥妤�(y猫)纰╁＋鐮旂┒鐢熻€屽鏂界殑鍏锋湁閬告嫈
鍔熻兘鐨勬按骞宠€冭│銆�
浜�銆佽€冭│瑕佹眰
鐢熺墿鍖栧鑸囧垎瀛愮敓鐗╁鏄敓鍛界瀛哥殑鍩虹鐞嗚珫瑾�锛岃姹傚鐢熸瘮杓冪郴绲�(t菕ng)鍦扮悊瑙ｅ拰鎺屾彙鐢熺墿
鍖栧鐨勫熀鏈蹇靛拰鍩烘湰鐞嗚珫锛涙帉鎻″悇椤炵敓鍖栫墿璩�(zh矛)鐨勭祼(ji茅)妲�(g貌u)銆佹€ц唱(zh矛)銆佸姛鑳藉強鍏跺悎鎴愪唬璎濆拰鍒嗚В浠�
璎濈殑鍩烘湰閫斿緫鍜岃(di脿o)鎺ф柟娉�锛涚悊瑙ｅ熀鍥犺〃閬斻€佽(di脿o)鎺у拰鍩哄洜宸ョ▼鐨勫熀鏈悊璜�锛涜兘缍滃悎閬嬬敤鎵€瀛哥殑
鐭ヨ瓨鍒嗘瀽鍟忛鍜岃В姹哄晱椤�銆傝姹傝€冪敓鑳界珯鍦ㄥ绉戠殑鍓嶆部锛屾妸鎻″绉戠殑閫插睍锛岄潏娲婚亱鐢ㄦ墍瀛哥殑
鐢熺墿鍖栧鐭ヨ瓨寰炲垎瀛愭按骞宠獚璀樺拰瑙ｉ噵鐢熷懡閬庣▼涓墍鐧�(f膩)鐢熺殑鐝�(xi脿n)璞�銆�
涓�銆佽€冭│鍏�(n猫i)瀹�
锛堜竴锛夎泲鐧借唱(zh矛)鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳�
1. 鎺屾彙铔嬬櫧璩�(zh矛)鐨勫熀鏈祫鎴愬柈浣�锛�20 绋� L-伪-姘ㄥ熀閰�锛岀啛鎮夋皑鍩洪吀鐨勯€氬紡鑸囩祼(ji茅)妲�(g貌u)鐗归粸銆傛帉
鎻℃皑鍩洪吀鐨勫叐鎬цВ闆㈠拰绱鍚告敹鎬ц唱(zh矛)銆傛帉鎻¤偨閸靛強澶氳偨閺堢殑閫ｆ帴鏂瑰紡锛屼簡瑙ｇ敓鐗╂椿鎬ц偨銆�
2. 鎺屾彙铔嬬櫧璩�(zh矛)鐨勫垎瀛愮祼(ji茅)妲�(g貌u)锛屽寘鎷偨鍠厓鍙婁竴绱氥€佷簩绱氱祼(ji茅)妲�(g貌u)锛岋紙伪-铻烘棆銆佄�-鎶樼枈銆佄�-杞�(zhu菐n)瑙�銆�
鐒¤(gu墨)鍗锋洸锛�锛屼笁绱�銆佸洓绱氱祼(ji茅)妲�(g貌u)姒傚康鍜岀董鎸侀嵉銆傛帉鎻℃ā搴�銆佺祼(ji茅)妲�(g貌u)鍩熴€佸垎瀛愪即渚舵蹇�銆�
3. 鐔熸倝铔嬬櫧璩�(zh矛)鍚勭礆绲�(ji茅)妲�(g貌u)鑸囧姛鑳介棞绯�锛岀啛鎮夎绱呰泲鐧界殑鍒嗗瓙绲�(ji茅)妲�(g貌u)銆傝绱呰泲鐧界┖闁撶祼(ji茅)妲�(g貌u)鑸�
閬嬫哀鍔熻兘闂滅郴銆傛帉鎻″崝(xi茅)鍚屾晥鎳�銆佸垾妲�(g貌u)鏁堟噳鐨勬蹇点€�
4. 鎺屾彙铔嬬櫧璩�(zh矛)鍏╂€ч浕闆�銆佽Κ姘磋啝楂�銆佽畩鎬�銆佺传澶栧惛鏀剁瓑鎬ц唱(zh矛)鍙婄浉闂滄蹇�锛岀啛鎮夎泲鐧借唱(zh矛)
鍒嗛洟绱斿寲鏂规硶鍜屼緷鎿�(j霉)鐨勬€ц唱(zh矛)銆備簡瑙ｅ鑲介張姘ㄥ熀閰告脯搴忕殑鍘熺悊銆�
5. 鐔熸倝铔嬬櫧璩�(zh矛)绲勫鐨勬蹇靛強鐮旂┒鏂规硶銆�
锛堜簩锛夋牳閰哥殑绲�(ji茅)妲�(g貌u)鑸囧姛鑳�
1. 鎺屾彙鏍歌嫹閰稿垎瀛愮祫鎴愬強绲�(ji茅)妲�(g貌u)锛孌NA銆丷NA 绲勬垚鐨勭暟鍚�銆�
2. 鎺屾彙鏍搁吀锛圖NA銆丷NA锛夌殑涓€绱氱祼(ji茅)妲�(g貌u)锛岄€ｆ帴閸点€�
3. 鎺屾彙 DNA 闆欒灪鏃嬬祼(ji茅)妲�(g貌u)妯″紡鐨勮榛�锛孌NA 鐨勮秴铻烘棆绲�(ji茅)妲�(g貌u)鍜屽姛鑳姐€�
4. 鎺屾彙 tRNA銆乵RNA銆乺RNA 鐨勭祫鎴�銆佺祼(ji茅)妲�(g貌u)鐗归粸鍜屽姛鑳�銆�
5. 鐔熸倝浠ヤ笅姒傚康锛氳瀺瑙ｆ韩搴�銆佸鑹叉晥鎳�銆丏NA 璁婃€у拰寰╂€�銆佹牳閰稿垎瀛愰洔浜�銆佹帰閲濇妧琛�銆�
锛堜笁锛夐叾
1. 鎺屾彙閰剁殑姒傚康锛涢叾鐨勫皥涓€鎬у強鍒嗛锛涢叾鐨勫偓鍖栨鍒�銆傛帉鎻＄祼(ji茅)鍚堥叾銆佽紨閰惰垏杓斿熀鐨勬
蹇�锛涚啛鎮夌祼(ji茅)鍚堥叾涓紨閰�銆侀噾灞洟瀛愮殑浣滅敤銆傛帉鎻℃椿鎬т腑蹇冦€佸繀闇€鍩哄湗鐨勬蹇�锛�
2. 鎺屾彙閰剁殑鐗圭暟鎬�锛岄叾鍙嶆噳鐗归粸銆備簡瑙ｉ叾-搴曠墿涓枔寰╁悎鐗╁瑾�銆�
3. 鎺屾彙褰遍熆閰跺偓鍖栭€熷害鐨勫洜绱狅紝绫虫皬甯告暩(sh霉) Km锛屾渶澶у弽鎳夐€熷害 Vmax 姒傚康鍙婃剰缇╋紱鏈€閬�
pH,鏈€閬╂韩搴�锛涚鐖€ф姂鍒跺姂绲�(ji茅)妲�(g貌u)浣滅敤鐗归粸锛涚啛鎮変笉鍚岄鍨嬪彲閫嗘姂鍒朵綔鐢ㄧ殑鍕曞姏瀛哥壒榛�銆�
4. 鎺屾彙闂滈嵉閰�銆佽畩妲�(g貌u)閰剁殑姒傚康銆傞叾娲绘€ц畩妲�(g貌u)瑾�(di脿o)绡€(ji茅)鐨勬鐞�锛屽叡鍍逛慨椋剧殑姒傚康銆傜啛鎮夊崝(xi茅)鍚�
鏁堟噳鐨勬蹇�銆傛帉鎻￠叾鍘熴€侀叾鍘熸縺娲荤殑姒傚康锛涘悓宸ラ叾鐨勬蹇碉紱鐔熸倝閰惰泲鐧藉悎鎴愯獦灏庤垏闃婚亸姒傚康
5. 浜嗚В閰惰垏閱�(y墨)瀛哥殑闂滅郴
锛堝洓锛夌董鐢熺礌鑸囪紨鍥犲瓙
1. 鎺屾彙缍敓绱犵殑姒傚康銆備簡瑙ｇ董鐢熺礌鐨勫垎椤炲強鐩搁棞鐨勭己涔忕櫏
2. 鐞嗚В缍敓绱犲湪浠ｈ瑵銆佺敓鐗╂哀鍖栧強閬哄偝淇℃伅鍌抽仦涓殑浣滅敤
3. 鎺屾彙姘存憾鎬х董鐢熺礌鐨勭敓鍖栦綔鐢�锛屾按婧舵€х董鐢熺礌鐨勮紨閰跺舰寮忓強浣滅敤銆傝憠閰�銆佺董鐢熺礌 B12
缂轰箯鑸囨儭鎬ц钵琛€銆傛帉鎻＄董鐢熺礌 C 鐨勭敓鍖栦綔鐢�锛屽琛€鐥�銆�
锛堜簲锛夌硸浠ｈ瑵
1. 鎺屾彙绯栫殑鐢熺悊鍔熻兘锛屾秷鍖栧惛鏀堕亷绋�銆侀儴浣嶅拰閰堕銆�
2. 鎺屾彙绯栭叺瑙ｇ殑涓昏閬庣▼锛岄棞閸甸叾锛岃(di脿o)绡€(ji茅)鏂瑰紡锛岀敓鐞嗘剰缇�銆�
3. 鎺屾彙绯栫殑鏈夋哀姘у寲鐨勪富瑕侀亷绋�锛岄棞閸甸叾锛岃(di脿o)绡€(ji茅)鏂瑰紡锛岀敓鐞嗘剰缇�銆傛帉鎻′笁缇ч吀寰挵(hu谩n)鐨�
閬庣▼锛岀壒榛�锛屾剰缇╁強瑾�(di脿o)绡€(ji茅)銆�
4. 鎺屾彙纾烽吀鎴婄硸閫斿緫姘у寲闅庢閬庣▼锛孨ADPH 鍜� 5-纾烽吀鏍哥硸鐢�(ch菐n)鐢熺殑鐢熺悊鎰忕京銆�
5. 鎺屾彙绯栧師鍚堟垚鍒嗚В鐨勫熀鏈亷绋嬶紝闂滈嵉閰�锛岃(di脿o)绡€(ji茅)鏂瑰紡銆傝倢绯栧師鍜岃倽绯栧師浠ｈ瑵鐨勭暟鍚�銆�
6. 鎺屾彙绯栫暟鐢熺殑姒傚康锛屽熀鏈亷绋嬶紝鐢熺悊鎰忕京锛涗钩閰稿惊鐠�(hu谩n)鐨勬蹇�銆備簡瑙ｇ硸鐣扮敓瑾�(di脿o)绡€(ji茅)鐗归粸銆�
7. 鎺屾彙琛€绯栨蹇碉紝琛€绯栫殑渚嗘簮鍘昏矾锛岃儼宄剁礌灏嶈绯栫殑瑾�(di脿o)绡€(ji茅)姗熺悊銆傜啛鎮夎儼楂樿绯栫礌銆佺硸
鐨唱(zh矛)婵€绱犲崌楂樿绯栨鐞嗐€備簡瑙ｈ绯栨按骞崇暟甯�銆�
锛堝叚锛夎剛椤炰唬璎�
1. 鎺屾彙鑴傝偑娑堝寲鍚告敹鐨勬浠�锛岃喗姹侀吀楣藉強杓旇剛閰剁殑浣滅敤锛屼钩绯滃井绮掔殑褰㈡垚銆傜啛鎮夎剛椤�
娑堝寲鍚告敹閬庣▼銆備簡瑙ｈ剛椤炵殑鍒嗛鍔熻兘銆�
2. 鎺屾彙鐢樻补涓夐叝鍚堟垚閬庣▼銆傝剛鑲嫊鍝＄殑姒傚康锛岄檺閫熼叾鍙婅(di脿o)绡€(ji茅)銆傛帉鎻＄敇娌逛唬璎濆強鑴傝偑閰� 尾-
姘у寲鐨勫叏閬庣▼锛岄棞閸甸叾鍙婅兘閲忕敓鎴�銆�
3. 鎺屾彙閰珨鐨勬蹇�锛屽悎鎴愬強鍒╃敤鐨勯儴浣�銆侀亷绋嬪拰鐢熺悊鎰忕京銆�
4. 鎺屾彙鑴傝偑閰稿悎鎴愮殑鍘熸枡锛岄棞閸甸叾鍙婅(di脿o)绡€(ji茅)銆傚繀闇€鑴傝偑閰哥殑姒傚康銆傜啛鎮夎剛鑲悎鎴愰亷绋�銆�
浜嗚В鑴傞吀鍏跺畠姘у寲鐨勬柟寮忥紝涓嶉＝鍜岃剛鑲吀鐨勯噸瑕佽鐢熺墿銆�
5. 鎺屾彙纾疯剛鐨勫垎椤炪€佺敇娌圭７鑴傜殑鍚堟垚鍙婇檷瑙ｉ€斿緫銆備簡瑙ｉ灅鑴傜殑鍒嗛銆佸悎鎴�銆�
6. 鎺屾彙鑶藉浐閱囧悎鎴愪唬璎濈殑鍘熸枡銆侀棞閸甸叾鍙婅(di脿o)绡€(ji茅)銆傝喗鍥洪唶鐨勮綁(zhu菐n)鍖�銆�
7. 鎺屾彙琛€婕胯剛铔嬬櫧鍒嗛鍙婄祫鎴�锛岃級鑴傝泲鐧界敓鐞嗗姛鐢�銆傚洓绋剛铔嬬櫧鐨勪唬璎濇娉�銆備簡瑙ｉ珮
鑴傝泲鐧借鐧ョ殑鍒嗗瀷鍙婅鑴傜暟甯搞€�
锛堜竷锛夌敓鐗╂哀鍖�
1. 鎺屾彙闆诲瓙鍌抽仦閺堢殑姒傚康锛岀祫鍒嗭紝鎺掑垪闋嗗簭锛屽叐姊濋浕瀛愬偝閬為張銆�
2. 鎺屾彙搴曠墿姘村钩纾烽吀鍖栬垏姘у寲纾烽吀鍖栫殑姒傚康銆�
3. 鎺屾彙 ATP 鍚堟垚鍋惰伅(li谩n)閮ㄤ綅銆傜啛鎮� ATP 鍚堥叾绲�(ji茅)妲�(g貌u)锛孉TP 鍚堟垚鍋惰伅(li谩n)姗熺悊銆傜啛鎮夊奖闊挎哀鍖栫７閰�
鍖栫殑鍥犵礌銆�
4. 鐔熸倝 ATP 寰挵(hu谩n)锛岄珮鑳界７閰搁嵉椤炲瀷锛岃帛瀛樺拰杞�(zhu菐n)绉�銆�
5. 鎺屾彙 NADH 杞�(zhu菐n)閬嬬殑鍏╃ó绌挎姗熷埗銆傜啛鎮� ATP/ADP 杞�(zhu菐n)閬�銆�
6. 鐔熸倝閬庢哀鍖栫墿閰�銆丼OD 鍜屽姞鍠哀閰�銆備簡瑙ｅ叾瀹冩哀鍖栭珨绯婚叾椤�銆�
锛堝叓锛夋皑鍩洪吀浠ｈ瑵
1. 鎺屾彙姘钩琛″強蹇呴渶姘ㄥ熀閰哥殑姒傚康锛岀啛鎮夎泲鐧借唱(zh矛)鐨勭敓鐞嗗姛鑳�銆�
2. 鎺屾彙铔嬬櫧璩�(zh矛)鐨勮厫鏁椾綔鐢ㄥ強鑵愭晽鐢�(ch菐n)鐗┿€傜啛鎮夎泲鐧借唱(zh矛)娑堝寲涓悇绋叾鐨勪綔鐢ㄥ強 纬-璋锋皑?锛�?br/>寰挵(hu谩n)銆傜啛鎮夐珨鍏�(n猫i)铔嬬櫧璩�(zh矛)闄嶈В鐨勪富瑕侀€斿緫銆�
3. 鎺屾彙姘ㄥ熀閰歌劔姘ㄥ熀浣滅敤鏂瑰紡锛氳綁(zhu菐n)姘ㄥ熀锛孡-璋锋皑閰告哀鍖栬劔姘ㄥ熀锛岃伅(li谩n)鍚堣劔姘ㄥ熀锛屽熀鏈亷绋�銆�
鐔熸倝 伪-閰吀浠ｈ瑵姒傛硜銆�
4. 鎺屾彙姘ㄧ殑渚嗘簮鍜屽幓璺�锛屾皑鐨勮綁(zhu菐n)閬嬮亷绋�锛屼笝姘ㄩ吀-钁¤悇绯栧惊鐠�(hu谩n)銆傛帉鎻″翱绱犵敓鎴愰偿姘ㄩ吀寰�
鐠�(hu谩n)鐨勯亷绋嬨€侀儴浣嶅強瑾�(di脿o)绡€(ji茅)銆�
5. 鎺屾彙姘ㄥ熀閰歌劔缇у熀浣滅敤锛岀敓鎴愮殑鐢熺悊娲绘€х墿璩�(zh矛)銆�
6. 鎺屾彙涓€纰冲柈浣嶇殑姒傚康銆佽級楂斿強鐢熺悊鍔熻兘銆�
7. 鎺屾彙娲绘€х敳鍩虹殑褰㈠紡銆傜啛鎮夌敳纭皑閰稿惊鐠�(hu谩n)鍜岃倢閰稿悎鎴�銆備簡瑙ｇ敱鑻笝姘ㄩ吀鍜岄叒姘ㄩ吀鐢�
鎴愮殑鐢熺悊娲绘€х墿璩�(zh矛)銆�
锛堜節锛夋牳鑻烽吀浠ｈ瑵
1. 鎺屾彙鍢屽懁鏍歌嫹閰稿緸闋悎鎴愰€斿緫锛氭蹇�銆佸師鏂欍€侀棞閸甸叾鍙婇亷绋�銆傜啛鎮夋牳鑻烽吀鐢熺墿鍔熻兘銆�
鍢屽懁鏍歌嫹閰歌鏁戝悎鎴愰€斿緫銆備簡瑙ｆ牳閰哥殑娑堝寲銆�
2. 鎺屾彙鑴哀鏍歌嫹閰哥殑鐢熸垚锛屾牳绯栨牳鑻烽吀閭勫師閰剁殑鎴愬垎銆�
3. 鎺屾彙鍢屽懁鏍歌嫹閰稿垎瑙ｄ唬璎濈祩鐢�(ch菐n)鐗�锛涚啛鎮夊槍鍛ゆ牳鑻烽吀鎶椾唬璎濈墿浣滅敤銆傜棝棰ㄧ櫏鐨勫師鍥犲強
娌荤檪鍘熷墖銆�
4. 鎺屾彙鍢у暥鏍歌嫹閰稿緸闋悎鎴愰€斿緫锛氭蹇�銆佸師鏂�銆侀棞閸甸叾鍙婇亷绋�銆傛帉鎻¤劔姘ц兏鑵哄槯鍟舵牳
鑻烽吀鐨勭敓鎴愩€�
5. 鐔熸倝鍢у暥鏍歌嫹閰歌鏁戝悎鎴愰€斿緫锛屽槯鍟舵牳鑻烽吀鎶椾唬璎濈墿浣滅敤銆�
6. 鐔熸倝鏍歌嫹閰哥殑杞�(zhu菐n)璁婇棞绯�銆傛牳鑻烽吀鍚堟垚瑾�(di脿o)绡€(ji茅)鐨勫熀鏈柟寮忋€�
锛堝崄锛夌墿璩�(zh矛)浠ｈ瑵鐨勮伅(li谩n)绯昏垏瑾�(di脿o)绡€(ji茅)
1. 鐔熸倝楂斿収(n猫i)鐗╄唱(zh矛)浠ｈ瑵鐨勭壒榛�銆�
2. 鎺屾彙绯栥€佽剛椤�銆佽泲鐧借唱(zh矛)涓夊ぇ鐗╄唱(zh矛)鍦ㄨ兘閲忎唬璎濄€佺墿璩�(zh矛)浠ｈ瑵闁撶殑鐩镐簰褰遍熆鍜屼簰鐩歌綁(zhu菐n)鍖栨
鍒�銆�
3. 鐔熸倝涓昏绲勭箶鍣ㄥ畼鐨勪唬璎濈壒榛�銆�
4. 鎺屾彙绱拌優姘村钩浠ｈ瑵瑾�(di脿o)绡€(ji茅)姒傚康銆傞棞閸甸叾銆侀叾鐨勮畩妲�(g貌u)瑾�(di脿o)绡€(ji茅)銆侀叾鐨勫寲瀛镐慨椋捐(di脿o)绡€(ji茅)姒傚康銆佺敓
鐞嗘剰缇╁拰鐗归粸銆傜啛鎮夐椁撳拰鎳夋縺鏅傛楂旂殑鏁撮珨瑾�(di脿o)绡€(ji茅)姒傛硜銆備簡瑙ｉ叾閲忚(di脿o)绡€(ji茅)鍜屾縺绱犳按骞宠(di脿o)绡€(ji茅)姒傚康銆�
锛堝崄涓€锛夎娑茬殑鐢熺墿鍖栧
1. 鎺屾彙琛€婕胯泲鐧界殑鍔熻兘銆傜啛鎮夎婕胯泲鐧界殑鍒嗛鍜屼富瑕佹€ц唱(zh矛)銆�
2. 鎺屾彙绱呯窗鑳炵硸浠ｈ瑵鐗归粸銆�2锛�3-浜岀７閰哥敇娌归吀鏃佽矾锛孨ADH 鍜� NADPH 鐨勫姛鑳�銆�
3. 鎺屾彙琛€绱呯礌鍚堟垚鍘熸枡銆侀棞閸甸叾銆佷富瑕侀亷绋�銆傜啛鎮夎绱呯礌鍚堟垚瑾�(di脿o)绡€(ji茅)锛岀櫧绱拌優鐨勪唬璎濄€�
锛堝崄浜岋級鑲濈殑鐢熺墿鍖栧
1. 鎺屾彙鑲濊嚐鍦ㄧ硸銆佽剛椤炲拰铔嬬櫧璩�(zh矛)浠ｈ瑵涓噸瑕佷綔鐢�銆傜啛鎮夎倽鍦ㄧ董鐢熺礌銆佹縺绱犱唬璎濅腑鐨勪綔
鐢�銆�
2. 鎺屾彙鐢熺墿杞�(zhu菐n)鍖栦綔鐢ㄧ殑姒傚康銆傝倽鐢熺墿杞�(zhu菐n)鍖栧弽鎳夌殑椤炲瀷锛屽姞鍠哀閰剁郴绲勬垚銆佷綔鐢�銆傜祼(ji茅)鍚�
鍙嶆噳鐨勭ó椤�锛岀祼(ji茅)鍚堝熀鍦樻椿鎬т緵楂�銆侀叾椤炪€傜啛鎮夊奖闊跨敓鐗╄綁(zhu菐n)鍖栧洜绱�銆�
3. 鎺屾彙鑶芥眮閰稿垎椤�锛屽垵绱氳喗姹侀吀鍚堟垚锛岃喗姹侀吀鑵歌倽寰挵(hu谩n)銆備簡瑙ｈ喗姹侀吀鍔熻兘銆�
4. 鎺屾彙鑶借壊绱犵敓鎴�銆佽綁(zhu菐n)閬嬮亷绋嬨€傝喗绱呯礌鍦ㄨ倽鑷熻綁(zhu菐n)璁�銆傝喗绱呯礌鑵搁亾涓畩鍖�锛岃喗鑹茬礌鑵歌倽
寰挵(hu谩n)銆傛父闆㈣喗绱呯礌鑸囩祼(ji茅)鍚堣喗绱呯礌銆傜啛鎮夐粌鐤哥殑姒傚康锛屽悇鍨嬮粌鐤哥殑鐥呭洜鍙婅嚚搴婄壒寰�銆�
锛堝崄涓夛級DNA 鐨勭敓鐗╁悎鎴�
1. 鎺屾彙涓績娉曞墖銆佸熀鍥犺〃閬斻€佸崐淇濈暀寰╁埗鐨勬蹇�銆�
2. 鎺屾彙鍙冭垏 DNA 寰╁埗鐨勪富瑕佺墿璩�(zh矛)鍙婂叾浣滅敤姗熺悊銆傛帉鎻� DNA 鑱氬悎閰朵綔鐢ㄧ壒榛�锛屽師鏍哥敓鐗�
鍜岀湡鏍哥敓鐗� DNA 鑱氬悎閰剁殑鐣板悓銆傛嫇瑁滅暟妲�(g貌u)閰�銆佸紩鐗╅叾浣滅敤銆傜啛鎮� DNA 寰╁埗鐨勬柟鍚戞€�銆佷繚鐪�
鎬�銆傜啛鎮夐€ｆ帴閰朵綔鐢ㄦ鐞嗐€�
3. 鎺屾彙 DNA 寰╁埗閬庣▼鍙婂悇闅庢鐨勭壒榛�銆傜绮掑拰绔矑閰舵蹇靛強浣滅敤銆傜啛鎮夊京鍒惰捣濮嬪拰宀�
宕庣墖娈�銆佸紩鐧�(f膩)楂�銆佽矤瓒呰灪鏃嬫蹇�銆佸舰鎴�銆備簡瑙ｆ痪鐠�(hu谩n)寰╁埗閬庣▼銆�
4. 鎺屾彙绐佽畩姒傚康锛孌NA 鎼嶅偡鐨勯鍨�锛屽垏闄や慨寰╃殑鍩烘湰鍘熺悊锛涚啛鎮夌獊璁婄殑鎰忕京銆佸紩鐧�(f膩)鍥�
绱犮€傜啛鎮夊厜淇京銆丼OS 淇京鍙婇噸绲勪慨寰╃殑姒傚康銆�
5. 鎺屾彙閫嗚綁(zhu菐n)閷勬蹇�銆佷綔鐢ㄩ亷绋嬨€傞€嗚綁(zhu菐n)閷勯叾浣滅敤鐗归粸銆傜敓鐗╁鎰忕京鍙婃噳鐢�銆�
锛堝崄鍥涳級RNA 鐨勭敓鐗╁悎鎴�
1. 鎺屾彙杞�(zhu菐n)閷勭殑姒傚康锛屼笉灏嶇ū杞�(zhu菐n)閷�銆佹ā鏉块張銆佺法纰奸張銆傚師鏍哥敓鐗� RNA 鑱氬悎閰跺叏閰讹紝鏍稿績
閰剁殑绲勬垚鍜屼綔鐢ㄣ€傜湡鏍哥敓鐗� RNA 鑱氬悎閰剁殑涓昏椤炲瀷鍜岀敘(ch菐n)鐗�銆�
2. 鎺屾彙 RNA 鑱氬悎閰惰垏妯℃澘杈ㄨ獚绲�(ji茅)鍚堛€傛帉鎻″師鏍歌綁(zhu菐n)閷勮捣濮�銆傜啛鎮夌湡鏍歌綁(zhu菐n)閷勫洜瀛�锛岃綁(zhu菐n)閷勫墠
璧峰寰╁悎鐗�銆傜啛鎮夊欢闀疯垏鍘熸牳鍏╅杞�(zhu菐n)閷勭祩姝㈤亷绋嬨€�
3. 鎺屾彙鐪熸牳鍩哄洜鐨勬柗瑁傚熀鍥�銆佸収(n猫i)鍚瓙銆佸椤瓙鐨勬蹇�銆傛帉鎻� mRNA銆乼RNA 杞�(zhu菐n)閷勫悗鐨勫姞
宸ユ柟寮�銆傜啛鎮夊収(n猫i)鍚瓙鍓帴姗熷埗锛宺RNA 鐨勫姞宸ラ亷绋�銆�
4. 鐔熸倝鏍搁叾鐨勬蹇�锛岀祼(ji茅)妲�(g貌u)銆佷綔鐢ㄧ壒榛�銆�
锛堝崄浜旓級铔嬬櫧璩�(zh矛)鐨勭敓鐗╁悎鎴�
1. 鎺屾彙缈昏鐨勬蹇靛強鍙冨姞铔嬬櫧璩�(zh矛)鐢熺墿鍚堟垚鐨勭墿璩�(zh矛)锛宮RNA銆乼RNA 鍙婃牳绯栭珨鐨勭祼(ji茅)妲�(g貌u)鍜屽湪
缈昏涓綔鐢紝姘ㄥ熀閰�-tRNA 鐨勭敓鎴�銆傜啛鎮夐伜鍌冲瘑纰肩殑鐗归粸銆�
2. 鎺屾彙鍘熸牳鍙婄湡鏍哥敓鐗╃炕璀殑鍩烘湰閬庣▼锛岃捣濮嬮殠娈�锛屽欢闀烽殠娈电殑涓夊€嬫椹燂細娉ㄥ唺锛屾垚
鑲斤紝杞�(zhu菐n)浣嶃€傜祩姝㈤殠娈�銆傜啛鎮夎捣濮嬪洜瀛�銆佸欢闀峰洜瀛愬拰閲嬫斁鍥犲瓙鐨勭ó椤炲拰浣滅敤銆傚師鏍�銆佺湡鏍哥炕璀�
閬庣▼鐣板悓銆�
3. 鎺屾彙澶氳仛鏍哥硸楂旂殑姒傚康銆佹剰缇�銆傜炕璀悗鍔犲伐鐨勬蹇靛強鍔犲伐鏂瑰紡锛岀啛鎮夐珮绱氱祼(ji茅)妲�(g貌u)淇＞锛�
涓€绱氱祼(ji茅)妲�(g貌u)淇＞銆傝泲鐧借唱(zh矛)鍚堟垚鍚庨澏鍚戣几閫�銆�
4. 鐔熸倝甯哥敤鎶楃敓绱犵瓑鐗╄唱(zh矛)鎶戝埗缈昏鐨勬鐞�銆�
锛堝崄鍏級鍩哄洜琛ㄩ仈瑾�(di脿o)鎺�
1. 鐔熸倝鍩哄洜琛ㄩ仈鐨勬蹇�锛屾檪闁�銆佺┖闁撴€�锛屽熀鍥犺〃閬旀柟寮�锛岀敓鐗╁鎰忕京銆傛帉鎻″熀鍥犺綁(zhu菐n)閷�
婵€娲昏(di脿o)绡€(ji茅)鐨勫熀鏈绱�銆�
2. 鎺屾彙鍘熸牳鍩哄洜鎿嶇副瀛愮殑姒傚康銆佺祼(ji茅)妲�(g貌u)鍜屽姛鑳�锛屼钩绯栨搷绺卞瓙鐨勮矤鎬�銆佹鎬�銆佸崝(xi茅)瑾�(di脿o)瑾�(di脿o)绡€(ji茅)銆�
鑹叉皑閰告搷绺卞瓙銆傜啛鎮夐樆閬忚泲鐧界殑璨犺(di脿o)鎺�锛宑AMP 浠嬪皫鐨� CAP 鐨勬瑾�(di脿o)鎺�锛岃綁(zhu菐n)閷勮“娓涖€�
3. 鎺屾彙鐪熸牳鍩哄洜瑾�(di脿o)鎺ч爢寮忎綔鐢ㄥ厓浠跺拰鍙嶅紡浣滅敤鍥犲瓙鐨勬蹇�锛岀ó椤炪€傜湡鏍歌綁(zhu菐n)閷勫洜瀛愮祼(ji茅)妲�(g貌u)锛�
PIC 鐨勮閰嶉亷绋�銆傜啛鎮夌湡鏍稿熀鍥犵祫绲�(ji茅)妲�(g貌u)鐗归粸锛岀湡鏍稿熀鍥犺〃閬旇(di脿o)鎺х壒榛炪€�
锛堝崄涓冿級鍩哄洜閲嶇祫鑸囧熀鍥犲伐绋�
1. 鐔熸倝鑷劧鐣屽熀鍥犺綁(zhu菐n)绉诲強閲嶇祫鐨勬柟寮�銆傜祼(ji茅)鍚�銆佽綁(zhu菐n)鍖栧強杞�(zhu菐n)灏庛€佽綁(zhu菐n)搴�銆佸熀鍥犻噸绲勩€�
2. 鎺屾彙閲嶇祫 DNA 鎶€琛撶殑鐩搁棞姒傚康锛屽熀鏈師鐞嗐€傞檺鍒舵€у収(n猫i)鍒囬叾姒傚康鍙婁綔鐢ㄧ壒榛�銆傜啛鎮夊父
鐢ㄨ級楂斿強鐗归粸銆傜洰鐨勫熀鍥犵殑鐛插彇鍙婅垏杓夐珨閫ｆ帴鐨勬柟娉�銆�
3. 鐔熸倝閲嶇祫楂旂殑灏庡叆鍙楅珨绱拌優鍙婄閬告柟娉�?锛涱媰铓╁鈥濈_鎶€琛�銆�
4. 鐔熸倝閲嶇祫 DNA 鎶€琛撳湪閱�(y墨)瀛镐腑鎳夌敤銆�
锛堝崄鍏級绱拌優淇℃伅鍌抽仦
1. 鎺屾彙绱拌優淇℃伅鍌抽仦姒傚康銆傜窗鑳為枔淇℃伅鐗╄唱(zh矛)锛岀窗鑳炲収(n猫i)淇℃伅鐗╄唱(zh矛)绋銆�
2. 鎺屾彙鑶滃彈楂旂ó椤炪€佺祼(ji茅)妲�(g貌u)銆傝優鍏�(n猫i)鍙楅珨绲�(ji茅)妲�(g貌u)銆侴 铔嬬櫧绲�(ji茅)妲�(g貌u)锛屽姛鑳�銆傜啛鎮夊彈楂斾綔鐢ㄧ壒榛炲強
瑾�(di脿o)绡€(ji茅)銆�
3. 鎺屾彙鑶滃彈楂斾粙灏庣殑淇℃伅鍌抽仦锛歝AMP-铔嬬櫧婵€閰堕€斿緫锛孋a2+-渚濊炒鎬ц泲鐧芥縺閰堕€斿緫锛宑GMP-
铔嬬櫧婵€閰堕€斿緫銆傜浜屼俊浣� cAMP 銆丏AG銆両P3銆乧GMP 鐢�(ch菐n)鐢熷強浣滅敤銆�
4. 鎺屾彙铔嬬櫧婵€閰� PKA銆丆a2+-CAM-PK銆丳KC銆丳KG 娲诲寲姗熷埗锛岃(di脿o)绡€(ji茅)浣滅敤銆�
5. 鎺屾彙閰皑閰歌泲鐧芥縺閰堕€斿緫姒傚康銆傚彈楂斿瀷 TPK 閫斿緫銆傛帉鎻＄窗鑳炲収(n猫i)鍙楅珨浠嬪皫鐨勪俊鎭偝閬�
閫斿緫銆傜啛鎮� JAK-STAT 閫斿緫銆佹牳鍥犲瓙 魏B 閫斿緫鐨勫弮鑸囨垚鍒嗗強瑾�(di脿o)绡€(ji茅)姗熷埗銆備簡瑙ｄ俊鎭€斿緫鐨勪氦浜掕伅(li谩n)
绯�銆�
锛堝崄涔濓級鐧屽熀鍥犮€佹姂鐧屽熀鍥犺垏鐢熼暦鍥犲瓙
1. 鎺屾彙鐧屽熀鍥犳蹇�銆傜梾姣掔檶鍩哄洜姒傚康銆佽嚧鐥呮鐞�銆傜窗鑳炵檶鍩哄洜鐗归粸鍜屽垎椤�銆�
2. 鎺屾彙鍘熺檶鍩哄洜鐨勬椿鍖栨鍒�锛屽師鐧屽熀鍥犵殑琛ㄩ仈鐢�(ch菐n)鐗╄垏鍔熺敤銆�
3. 鐔熸倝鎶戠檶鍩哄洜姒傚康銆侀鍨嬪拰浣滅敤姗熺悊銆�
4. 鐔熸倝鐢熼暦鍥犲瓙姒傚康銆佸垎椤炪€備簡瑙ｇ敓闀峰洜瀛愪綔鐢ㄦ鐞嗭紝绱拌優鍑嬩骸姒傚康鍜岀壒榛�銆�
锛堜簩鍗侊級鍩哄洜瑷烘柗鑸囧熀鍥犳不鐧�
1. 鐔熸倝鍩哄洜瑷烘柗鐨勬蹇�銆佺壒榛�銆傚熀鍥犺ê鏂风殑甯哥敤鎶€琛�銆備簡瑙ｅ熀鍥犺ê鏂峰湪閱�(y墨)瀛镐腑鎳夌敤銆�
2. 鐔熸倝鍩哄洜娌荤檪鐨勬蹇�锛岄鍨嬨€傜啛鎮夊熀鍥犳不鐧傜殑鍩烘湰绋嬪簭銆備簡瑙ｅ熀鍥犳不鐧傚湪閱�(y墨)瀛镐腑鎳�
鐢�銆�
锛堜簩鍗佷竴锛夊垎瀛愮敓鐗╁甯哥敤鎶€琛撹垏浜洪鍩哄洜绲勮▓鍔�
1. 鎺屾彙鍒嗗瓙闆滀氦鑸囧嵃婕妧琛撳師鐞�銆侀鍨�銆佹噳鐢ㄣ€�
2. 鎺屾彙 PCR 鎶€琛撳師鐞�锛屾噳鐢�銆�
3. 鐔熸倝鏍搁吀搴忓垪鍒嗘瀽鎶€琛撳師鐞�銆�
4. 浜嗚В鍩哄洜鏂囧韩銆佺敓鐗╄姱鐗囩殑鍩烘湰姒傚康鍙婃噳鐢ㄣ€�
5. 浜嗚В浜洪鍩哄洜绲勮▓鍔冨収(n猫i)瀹�銆佹剰缇�锛屽悗鍩哄洜绲勭爺绌跺収(n猫i)瀹广€傜柧鐥呯浉闂滃熀鍥犲厠闅�銆傚叾瀹冨熀
鍥犲伐绋嬫妧琛撱€�
鍏嶈铂鑱叉槑锛氭湰鏂囩郴杞�(zhu菐n)杓夎嚜缍�(w菐ng)绲�锛屽鏈変镜鐘�锛岃珛鑱�(li谩n)绯绘垜鍊戠珛鍗冲埅闄�锛屽彟锛氭湰鏂囧儏浠ｈ〃浣滆€呭€嬩汉瑙€榛烇紝鑸囨湰缍�(w菐ng)绔欑劇闂�銆傚叾鍘熷壍(chu脿ng)鎬т互鍙婃枃涓櫝杩版枃瀛楀拰鍏�(n猫i)瀹规湭缍�(j墨ng)鏈珯璀夊锛屽皪鏈枃浠ュ強鍏朵腑鍏ㄩ儴鎴栬€呴儴鍒嗗収(n猫i)瀹�銆佹枃瀛楃殑鐪熷鎬с€佸畬鏁存€�銆佸強鏅傛€ф湰绔欎笉浣滀换浣曚繚璀夋垨鎵胯锛岃珛璁€鑰呭儏浣滃弮鑰�锛屽苟璜嬭嚜琛屾牳瀵︾浉闂滃収(n猫i)瀹广€�
|
鏂囩珷鎼滅储
鎮ㄧ従(xi脿n)鍦ㄧ殑浣嶇疆锛� 
