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涓�銆佽€冭│褰㈠紡鍜岃│鍗风祼(ji茅)妲�(g貌u)
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锛堜竴锛夌敓鐗╁寲瀛�(xu茅)姒傝堪
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(1) 姘ㄥ熀閰哥殑绲�(ji茅)妲�(g貌u)閫氬紡;姘ㄥ熀閰哥殑鍒嗛锛涗笁瀛楃锛涙皑鍩洪吀閰稿牽鍖栧(xu茅)銆乸K 鍊�銆乸I锛�
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浜嗚В锛�
(1) 铔嬬櫧璩�(zh矛)鐨勬蹇佃垏鐢熺墿瀛�(xu茅)鎰忕京锛�
(2) 姘ㄥ熀閰哥殑鐢查啗婊村畾锛�
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鎺屾彙锛�
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(2) 绲勬垚铔嬬櫧璩�(zh矛)鐨� 20 绋皑鍩洪吀鐨勭祼(ji茅)妲�(g貌u)閫氬紡銆佷笁瀛楃锛涘悇姘ㄥ熀閰哥祼(ji茅)妲�(g貌u)鐗归粸锛涙皑鍩洪吀鍒嗛
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(7) Sanger 瑭﹀姂鍜� Edman 鍙嶆噳(y墨ng)锛涘伌(c猫)閺堝钒鍩虹殑鍖栧(xu茅)鍙嶆噳(y墨ng)锛�
(8) 骞剧ó涓昏铔嬬櫧閰剁殑浣滅敤閮ㄤ綅鍜岃泲鐧借唱(zh矛)姘ㄥ熀閰稿簭鍒楃⒑瀹氱殑鏂规硶锛岃兘鏍规摎(j霉)瀵﹂鎺ㄦ柗绨″柈
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褰遍熆锛涜泲鐧借唱(zh矛)鐨勬皑鍩洪吀搴忓垪鑸囩敓鐗╁姛鑳姐€�
锛堜笁锛夋牳閰稿寲瀛�(xu茅)
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2锛� 鍩烘湰瑕佹眰
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鎺屾彙锛�
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锛堝洓锛夐叾
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(5)B 鏃忕董鐢熺礌鑸囪紨閰�锛涚董鐢熺礌鍦ㄤ唬璎濅腑鐨勪綔鐢紱
2锛� 鍩烘湰瑕佹眰
浜嗚В锛�
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鎺屾彙锛�
(1)涓€浜涗富瑕佹按婧舵€х董鐢熺礌鐨勫悕绋�銆佺祼(ji茅)妲�(g貌u)銆佺敓鐞嗕綔鐢ㄥ拰瀹冨€戠殑杓旈叾褰㈠紡锛圢AD
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纭緵閰革級锛�
(2)閰跺偓鍖栦綔鐢ㄧ壒榛烇紱
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(4)搴曠墿婵冨害灏嶉叾淇冨弽鎳�(y墨ng)鐨勫奖闊匡紱
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(7)閰剁殑浣滅敤姗熷埗銆佽兘澶犺垑渚嬭鏄庨叾鐨勫垾妲�(g貌u)瑾�(di脿o)鎺у拰鍙€嗗叡鍍逛慨椋捐(di脿o)鎺э紱
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1锛庤€冩牳鐭ヨ瓨榛�
(1) D锛孡锛嶆(g貌u)鍨嬬殑鍛藉悕绯荤当(t菕ng)锛涢噸瑕佸绯栫殑绲勬垚锛涚暟闋墿锛埼� 鎴栁� 锛�锛涚硸鐨勮畩鏃嬬従(xi脿n)璞�锛涚硸鑻�
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(2) 澶氱硸鐩搁棞(gu膩n)姒傚康锛涢噸瑕佸绯栫殑绲勬垚锛涙穩绮�銆佺簴缍礌銆佺硸鍘�銆佺硸鑳鸿仛绯栫殑绲勬垚鍙婄祼(ji茅)妲�(g貌u)鐗归粸锛�
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2锛� 鍩烘湰瑕佹眰
浜嗚В锛�
(1) 绯栫殑涔欓唶鐧�(f膩)閰靛拰涔抽吀鐧�(f膩)閰碉紱
(2) 涓夌晶閰稿惊鐠�(hu谩n)鐨勮(di脿o)鎺�锛�
(3) 涔欓啗閰稿惊鐠�(hu谩n)锛�
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(5)绯栭鐢熺墿瀛�(xu茅)浣滅敤锛涚珛楂旂暟妲�(g貌u)楂�锛涘柈绯栫殑姘у寲锛涢噸瑕佺殑鍠硸琛嶇敓鐗�锛�
(6) 绯栭鐗╄唱(zh矛)鐨勭祫鎴�銆佺敓鐗╁(xu茅)浣滅敤鍙婄浉闂�(gu膩n)姒傚康锛�
(7)绯栭叺瑙ｇ殑瑾�(di脿o)绡€(ji茅)锛�
(8)绯栧師鍚堟垚浠ｈ瑵閫斿緫锛�
(9)绯栦唬璎濈殑瑾�(di脿o)绡€(ji茅)鎺у埗鍜屽咕绋厛澶╂€х硸浠ｈ瑵閰剁殑缂洪櫡鐥�锛�
鎺屾彙锛�
(1) 涓欓叜閰哥殑鍘昏矾锛�
(2) 涓夌晶閰稿惊鐠�(hu谩n)姝风▼鍜岀７閰告垗绯栭€斿緫闄愰€熼叾锛涗綔鐢ㄩ儴浣嶏紱
(3) 涓夌晶閰稿惊鐠�(hu谩n)鐨勭敓鐗╁(xu茅)鎰忕京锛�
(4) 纾烽吀鎴嶇硸閫斿緫鐨勭敓鐗╁(xu茅)鎰忕京锛�
(5) 绯栧師鐣扮敓浣滅敤锛�
(6)绯栭鐗╄唱(zh矛)鐨勫垎椤烇紱鍠硸鐨� D L锛嶆(g貌u)鍨嬬殑鍛藉悕绯荤当(t菕ng)锛涚暟闋墿锛埼� 鎴栁� 锛�锛涚硸鐨勮畩鏃嬬従(xi脿n)璞�锛�
(7)钄楃硸銆佷钩绯�銆侀亥鑺界硸鐨勫垎瀛愮祫鎴愬強閫ｆ帴鐨勭硸鑻烽嵉锛涢倓鍘熺硸鑸囬潪閭勫師绯�锛�
(8) 甯歌瀵＄硸鐨勭祫鎴愬強鐩搁棞(gu膩n)鎬ц唱(zh矛)锛�
(9) 娣€绮�銆佺簴缍礌銆佺硸鍘�銆佺硸鑳鸿仛绯栫殑绲勬垚鍙婄祼(ji茅)妲�(g貌u)鐗归粸锛�
(10)绯栧熀鏈蹇靛強鍒嗛锛�
(11) 钁¤悇绯栫劇姘ч檷姝风▼銆佷笝閰搁吀鐨勭敓鎴愰亷绋嬩腑鐨勯棞(gu膩n)鍋ユ椹熷拰闄愰€熼叾锛涚劇姘ч檷瑙ｅ拰鏈夋哀
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(12) 绯栧師鐣扮敓浣滅敤鍙婅(di脿o)鎺э紱
(13) 纾烽吀鎴婄硸閫斿緫涓昏鍙嶆噳(y墨ng)锛�
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锛堝叚锛変唬璎濈附璜�銆佺敓鐗╂哀鍖栧拰鐢熺墿鑳藉(xu茅)
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(1) 鐗╄唱(zh矛)浠ｈ瑵鑸囪兘閲忎唬璎濈殑闂�(gu膩n)绯伙紱ATP 鐨勭祼(ji茅)妲�(g貌u)鐗规€у強鐗规畩浣滅敤锛涚敓鐗╂哀鍖栭亷绋嬩腑浜屾哀鍖栫⒊
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(2) 姘у寲纾烽吀鍖栨蹇�銆佸懠鍚搁張鐨勭祫鎴愬強閬炴矮銆侀仦闆诲瓙鐨勯亷绋�锛涜兘閲忕殑鐢�(ch菐n)鐢�銆佽綁(zhu菐n)绉诲強鍎插瓨锛�
闆诲瓙鍌抽仦鍜� ATP 褰㈡垚鍋惰伅(li谩n)姗熺悊銆佸寲瀛�(xu茅)婊查€忓亣瑾殑瑕侀粸锛岃唱(zh矛)瀛愭績搴︽搴﹀樊鏄浣曞舰鎴愮殑锛�
2锛� 鍩烘湰瑕佹眰
浜嗚В锛�
(1) 楂樿兘纾烽吀鍖栧悎鐗╁強椤炲瀷銆丄TP 鐨勭祼(ji茅)妲�(g貌u)鐗规€у強鐗规畩浣滅敤锛�
(2) 鍖栧(xu茅)鍙嶆噳(y墨ng)涓嚜鐢辫兘鐨勮畩鍖栧拰鎰忕京锛�
(3) 鐢熺墿姘у寲涓兘閲忕殑鐢�(ch菐n)鐢�銆佽綁(zhu菐n)绉诲強鍎插瓨锛�
(4) 闆诲瓙鍌抽仦鍜� ATP 褰㈡垚鐨勫伓鑱�(li谩n)姗熺悊銆佽(di脿o)绡€(ji茅)姗熷埗锛�
(5) 鑳炴恫涓殑 NADH 杞�(zhu菐n)鎻涚偤绶氱矑楂斾腑鐨� NADH 鐨勯€斿緫銆傜窔绮掗珨绌挎绯荤当(t菕ng)锛�
鎺屾彙锛�
(1)鏂伴櫝浠ｈ瑵鐨勬剰缇�锛涚墿璩�(zh矛)浠ｈ瑵鑸囪兘閲忎唬璎濈殑闂�(gu膩n)绯�锛�
(2) 姘у寲纾烽吀鍖栨蹇�銆佸懠鍚搁張鐨勭祫鎴愬強閬炴矮銆侀仦闆诲瓙鐨勯亷绋�锛�
(3) P/O 姣�銆佽В鍋惰伅(li谩n)鍜屾姂鍒�銆佸寲瀛�(xu茅)婊查€忓亣瑾殑瑕侀粸锛岃唱(zh矛)瀛愭績搴︽搴﹀樊鏄浣曞舰鎴愮殑锛�
锛堜竷锛夎剛璩�(zh矛)浠ｈ瑵
1锛庤€冩牳鐭ヨ瓨榛�
(1) 鑴傝偑閰哥殑鍒嗚В浠ｈ瑵锛涜剛鑲按瑙ｇ殑闄愰€熼叾锛涚敇娌圭殑鍒嗚В锛�
(2) 閰珨鐨勬蹇碉紱鑴傝偑閰稿垎瑙ｄ唬璎濈殑瑾�(di脿o)绡€(ji茅)锛涢叜楂旂殑鍚堟垚鍜屽埄鐢紱
(3) 鍗佸叚纰充互涓嬮＝鍜岃剛鑲吀鐨勫悎鎴愰亷绋嬩互鍙婇檺閫熼叾锛涜剛鑲吀鍚堥叾寰�(f霉)鍚堥珨鐨勭祫鎴�锛�
(4) 鑴傝偑鐨勫悎鎴�锛涚７鑴傚拰鑶藉浐閱囦唬璎�锛�
2锛� 鍩烘湰瑕佹眰
浜嗚В锛�
(1) 鑴傞娑堝寲鍚告敹鍜岃綁(zhu菐n)閬�锛�
(2) 閰珨鐨勫悎鎴愬拰鍒╃敤浠ュ強閰珨鐢熸垚鐨勭敓鐞嗘剰缇�锛�
(3) 椋藉拰鑴傞吀鐨勭⒊閺堝欢闀烽€斿緫鑴傝偑閰哥⒊閺堢殑寤朵几鍙婁笉椋藉拰鑴傝偑閰哥殑鍚堟垚锛�
(4) 澶氫笉椋藉拰鑴傝偑閰哥殑閲嶈琛嶇敓鐗�锛岃剛鑲吀鍚堟垚鐨勮(di脿o)绡€(ji茅)锛�
鎺屾彙锛�
(1) 鑴傝泲鐧借垏杓夎剛铔嬬櫧锛�
(2) 鑴傝偑姘磋В鐨勯檺閫熼叾锛�
(3) 鐢樻补鍜岃剛鑲吀鐨勫垎瑙ｄ唬璎濆強鑳介噺鐢�(ch菐n)鐢熸儏娉併€�
(4) 閰珨鐨勬蹇�锛�
(5) 鑴傝偑閰稿垎瑙ｄ唬璎濈殑瑾�(di脿o)绡€(ji茅)锛�
(6) 鍗佸叚纰充互涓嬮＝鍜岃剛鑲吀鐨勫悎鎴愰亷绋嬩互鍙婇檺閫熼叾锛�
(7) 鑴傝偑閰稿悎閰跺京(f霉)鍚堥珨鐨勭祫鎴�锛�
(8) L-伪 -纾烽吀鐢樻补鐨勫悎鎴�銆佽剛鑲殑鍚堟垚锛�
(9) 纾疯剛鍜岃喗鍥洪唶浠ｈ瑵锛�
锛堝叓锛夋皑鍩洪吀鍜屾牳鑻烽吀鐨勪唬璎�
1锛庤€冩牳鐭ヨ瓨榛�
(1) 杞�(zhu菐n)姘ㄥ熀浣滅敤鍙婅綁(zhu菐n)姘ㄩ叾鐨勭壒榛�锛涜泲鐧介叾楂旈伕鎿囬檷瑙ｆ硾绱犲寲鐨勮泲鐧借唱(zh矛)锛�
(2) 鑴皑鍩轰綔鐢�锛涘翱绱犵殑褰㈡垚閬庣▼鍙婂舰鎴愰儴浣嶅強灏跨礌寰挵(hu谩n)鑸� TCA 寰挵(hu谩n)鐨勮伅(li谩n)绯�锛�
(3) ?-閰吀鐨勪唬璎�(纰抽鏋剁殑姘у寲)锛涚敓绯栨皑鍩洪吀鑸囩敓閰皑鍩洪吀锛�
(4) 鑻笝姘ㄩ吀鍜岄叒姘ㄩ吀浠ｈ瑵锛涙皑鍩洪吀鑸囩敓鐗╂椿鎬х墿璩�(zh矛)锛涙皑鍩洪吀鑸囦竴纰冲柈浣�锛�
(5) 鍢屽懁鏍歌嫹閰哥殑鍒嗚В浠ｈ瑵锛涘槯鍟舵牳鑻烽吀鐨勫垎瑙ｄ唬璎�锛�
(6) 鍢屽懁鏍歌嫹閰稿拰鍢у暥鏍歌嫹閰稿緸闋悎鎴�锛涜劔姘ф牳鑻烽吀鐨勫悎鎴�
2锛� 鍩烘湰瑕佹眰
浜嗚В锛�
(1)鑳炲収(n猫i)铔嬬櫧璩�(zh矛)闄嶈В鐨勬柟寮�锛�
(2)姗熼珨灏嶈泲鐧借唱(zh矛)鐨勬秷鍖栧惛鏀讹紱
(3)澶栨簮铔嬬櫧璩�(zh矛)鍜屽収(n猫i)婧愯泲鐧借唱(zh矛)鐨勯檷瑙�锛�
(4)姘ㄥ熀閰歌垏鐢熺墿娲绘€х墿璩�(zh矛)锛�
(5)鑳虹殑浠ｈ瑵鍜� CO2 鐨勫幓璺�锛�
(6)鍚～姘ㄥ熀閰哥殑浠ｈ瑵锛屽悇绋皑鍩洪吀鐨勫悎鎴�锛屾皑鍩洪吀閲嶈琛嶇敓鐗╃殑鐢熺墿鍚堟垚锛�
(7)鏍歌嫹閰搁叾鍜屾牳鑻烽叾锛�
(8)鏍歌嫹閰哥敓鐗╁悎鎴愮殑鍩烘湰閫斿緫锛涜紨閰舵牳鑻烽吀鐨勭敓鐗╁悎鎴�;
鎺屾彙锛�
(1)杞�(zhu菐n)姘ㄥ熀浣滅敤鍙婅綁(zhu菐n)姘ㄩ叾鐨勭壒榛烇紱
(2)姘ㄥ熀閰哥殑杞�(zhu菐n)姘ㄥ熀浣滅敤鍜岃伅(li谩n)鍚堣劔姘ㄥ熀浣滅敤锛�
(3)铔嬬櫧閰堕珨閬告搰闄嶈В娉涚礌鍖栫殑铔嬬櫧璩�(zh矛)锛�
(4)?-閰吀鐨勪唬璎�(纰抽鏋剁殑姘у寲)锛涚敓绯栨皑鍩洪吀鑸囩敓閰皑鍩洪吀
(5)鑻笝姘ㄩ吀鍜岄叒姘ㄩ吀浠ｈ瑵锛屾皑鍩洪吀鑸囩敓鐗╂椿鎬х墿璩�(zh矛)锛屾皑鍩洪吀鑸囦竴纰冲柈浣�锛�
(6)鍢屽懁鏍歌嫹閰哥殑鍒嗚В鍜屽槯鍟舵牳鑻烽吀鐨勫垎瑙ｄ唬璎濈敘(ch菐n)鐗╋紱
(7)闄愬埗鎬ф牳閰稿収(n猫i)鍒囬叾鐨勬蹇�锛�
(8)鍢屽懁鏍歌嫹閰稿拰鍢у暥鏍歌嫹閰稿緸闋悎鎴�锛�
(9)鏍歌嫹閰歌綁(zhu菐n)鍖栨垚鏍歌嫹涓夌７閰稿強鑴哀鏍歌嫹閰哥殑鍚堟垚锛�
(10) 灏跨礌鐨勫舰鎴愰亷绋嬪強褰㈡垚閮ㄤ綅鍙婂翱绱犲惊鐠�(hu谩n)鑸� TCA 寰挵(hu谩n)鐨勮伅(li谩n)绯�锛�
锛堜節锛夋牳閰稿拰铔嬬櫧璩�(zh矛)鐨勭敓鐗╁悎鎴�
1锛庤€冩牳鐭ヨ瓨榛�
(1)DNA 寰�(f霉)鍒堕亷绋嬶紱DNA 鐨勫崐涓嶉€ｇ簩(x霉)寰�(f霉)鍒跺拰 DNA 寰�(f霉)鍒堕珨绯�锛涢€嗚綁(zhu菐n)閷勯叾 鍦� DNA 鎸囧皫(d菐o)涓�
鐨� RNA 鍚堟垚閬庣▼锛涚湡鏍哥敓鐗╀腑 RNA 鐨勫姞宸�锛�
(2)铔嬬櫧璩�(zh矛)鍚堟垚鐨勫垎瀛愬熀绀�(ch菙)鍙婂悇鐗╄唱(zh矛)鐨勪綔鐢�锛涢伜鍌冲瘑纰肩殑鍩烘湰鐗规€э紱
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