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锛�3锛夊～绌洪 10-20%
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褰遍熆锛涜泲鐧借唱鐨勬皑鍩洪吀搴忓垪鑸囩敓鐗╁姛鑳�銆�
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2锛� 鍩烘湰瑕佹眰
浜嗚В锛�
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(1) D锛孡锛嶆鍨嬬殑鍛藉悕绯荤当(t菕ng)锛涢噸瑕佸绯栫殑绲勬垚锛涚暟闋墿锛埼� 鎴栁� 锛�锛涚硸鐨勮畩鏃嬬従(xi脿n)璞�锛涚硸鑻�
閸�銆佺硸鐨勭７閰歌剛锛涢倓鍘熺硸鑸囬潪閭勫師绯栵紱
(2) 澶氱硸鐩搁棞姒傚康锛涢噸瑕佸绯栫殑绲勬垚锛涙穩绮�銆佺簴缍礌銆佺硸鍘�銆佺硸鑳鸿仛绯栫殑绲勬垚鍙婄祼妲嬬壒榛烇紱
(3) 涓欓吀閰哥殑鐢熸垚閬庣▼涓殑闂滃仴姝ラ鍜岄檺閫熼叾锛涗綔鐢ㄩ儴浣�锛涚硸閰佃В鐢熺墿瀛告剰缇�锛�
2锛� 鍩烘湰瑕佹眰
浜嗚В锛�
(1) 绯栫殑涔欓唶鐧�(f膩)閰靛拰涔抽吀鐧�(f膩)閰�锛�
(2) 涓夌晶閰稿惊鐠�(hu谩n)鐨勮鎺�锛�
(3) 涔欓啗閰稿惊鐠�(hu谩n)锛�
(4) 绯栧師鍒嗚В浠ｈ瑵閫斿緫锛�
(5)绯栭鐢熺墿瀛镐綔鐢紱绔嬮珨鐣版楂�锛涘柈绯栫殑姘у寲锛涢噸瑕佺殑鍠硸琛嶇敓鐗�锛�
(6) 绯栭鐗╄唱鐨勭祫鎴�銆佺敓鐗╁浣滅敤鍙婄浉闂滄蹇碉紱
(7)绯栭叺瑙ｇ殑瑾跨瘈(ji茅)锛�
(8)绯栧師鍚堟垚浠ｈ瑵閫斿緫锛�
(9)绯栦唬璎濈殑瑾跨瘈(ji茅)鎺у埗鍜屽咕绋厛澶╂€х硸浠ｈ瑵閰剁殑缂洪櫡??锛�
鎺屾彙锛�
(1) 涓欓叜閰哥殑鍘昏矾锛�
(2) 涓夌晶閰稿惊鐠�(hu谩n)姝风▼鍜岀７閰告垗绯栭€斿緫闄愰€熼叾锛涗綔鐢ㄩ儴浣�锛�
(3) 涓夌晶閰稿惊鐠�(hu谩n)鐨勭敓鐗╁鎰忕京锛�
(4) 纾烽吀鎴嶇硸閫斿緫鐨勭敓鐗╁鎰忕京锛�
(5) 绯栧師鐣扮敓浣滅敤锛�
(6)绯栭鐗╄唱鐨勫垎椤�锛涘柈绯栫殑 D L锛嶆鍨嬬殑鍛藉悕绯荤当(t菕ng)锛涚暟闋墿锛埼� 鎴栁� 锛�锛涚硸鐨勮畩鏃嬬従(xi脿n)璞�锛�
(7)钄楃硸銆佷钩绯栥€侀亥鑺界硸鐨勫垎瀛愮祫鎴愬強閫ｆ帴鐨勭硸鑻烽嵉锛涢倓鍘熺硸鑸囬潪閭勫師绯栵紱
(8) 甯歌瀵＄硸鐨勭祫鎴愬強鐩搁棞鎬ц唱锛�
(9) 娣€绮�銆佺簴缍礌銆佺硸鍘�銆佺硸鑳鸿仛绯栫殑绲勬垚鍙婄祼妲嬬壒榛�锛�
(10)绯栧熀鏈蹇靛強鍒嗛锛�
(11) 钁¤悇绯栫劇姘ч檷姝风▼銆佷笝閰搁吀鐨勭敓鎴愰亷绋嬩腑鐨勯棞鍋ユ椹熷拰闄愰€熼叾锛涚劇姘ч檷瑙ｅ拰鏈夋哀
闄嶈В鐨勪笉鍚岄粸锛�
(12) 绯栧師鐣扮敓浣滅敤鍙婅鎺э紱
(13) 纾烽吀鎴婄硸閫斿緫涓昏鍙嶆噳锛�
鐞嗚В骞舵帉鎻★細
锛堝叚锛変唬璎濈附璜栥€佺敓鐗╂哀鍖栧拰鐢熺墿鑳藉
1锛庤€冩牳鐭ヨ瓨榛�
(1) 鐗╄唱浠ｈ瑵鑸囪兘閲忎唬璎濈殑闂滅郴锛汚TP 鐨勭祼妲嬬壒鎬у強鐗规畩浣滅敤锛涚敓鐗╂哀鍖栭亷绋嬩腑浜屾哀鍖栫⒊
鍜屾按鐨勭敓鎴�锛�
(2) 姘у寲纾烽吀鍖栨蹇�銆佸懠鍚搁張鐨勭祫鎴愬強閬炴矮銆侀仦闆诲瓙鐨勯亷绋�锛涜兘閲忕殑鐢�(ch菐n)鐢�銆佽綁绉诲強鍎插瓨锛�
闆诲瓙鍌抽仦鍜� ATP 褰㈡垚鍋惰伅(li谩n)姗熺悊銆佸寲瀛告徊閫忓亣瑾殑瑕侀粸锛岃唱瀛愭績搴︽搴﹀樊鏄浣曞舰鎴愮殑锛�
2锛� 鍩烘湰瑕佹眰
浜嗚В锛�
(1) 楂樿兘纾烽吀鍖栧悎鐗╁強椤炲瀷銆丄TP 鐨勭祼妲嬬壒鎬у強鐗规畩浣滅敤锛�
(2) 鍖栧鍙嶆噳涓嚜鐢辫兘鐨勮畩鍖栧拰鎰忕京锛�
(3) 鐢熺墿姘у寲涓兘閲忕殑鐢�(ch菐n)鐢�銆佽綁绉诲強鍎插瓨锛�
(4) 闆诲瓙鍌抽仦鍜� ATP 褰㈡垚鐨勫伓鑱�(li谩n)姗熺悊銆佽绡€(ji茅)姗熷埗锛�
(5) 鑳炴恫涓殑 NADH 杞夋彌鐐虹窔绮掗珨涓殑 NADH 鐨勯€斿緫銆傜窔绮掗珨绌挎绯荤当(t菕ng)锛�
鎺屾彙锛�
(1)鏂伴櫝浠ｈ瑵鐨勬剰缇�锛涚墿璩唬璎濊垏鑳介噺浠ｈ瑵鐨勯棞绯�锛�
(2) 姘у寲纾烽吀鍖栨蹇�銆佸懠鍚搁張鐨勭祫鎴愬強閬炴矮銆侀仦闆诲瓙鐨勯亷绋嬶紱
(3) P/O 姣�銆佽В鍋惰伅(li谩n)鍜屾姂鍒躲€佸寲瀛告徊閫忓亣瑾殑瑕侀粸锛岃唱瀛愭績搴︽搴﹀樊鏄浣曞舰鎴愮殑锛�
锛堜竷锛夎剛璩唬璎�
1锛庤€冩牳鐭ヨ瓨榛�
(1) 鑴傝偑閰哥殑鍒嗚В浠ｈ瑵锛涜剛鑲按瑙ｇ殑闄愰€熼叾锛涚敇娌圭殑鍒嗚В锛�
(2) 閰珨鐨勬蹇�锛涜剛鑲吀鍒嗚В浠ｈ瑵鐨勮绡€(ji茅)锛涢叜楂旂殑鍚堟垚鍜屽埄鐢�锛�
(3) 鍗佸叚纰充互涓嬮＝鍜岃剛鑲吀鐨勫悎鎴愰亷绋嬩互鍙婇檺閫熼叾锛涜剛鑲吀鍚堥叾寰╁悎楂旂殑绲勬垚锛�
(4) 鑴傝偑鐨勫悎鎴愶紱纾疯剛鍜岃喗鍥洪唶浠ｈ瑵锛�
2锛� 鍩烘湰瑕佹眰
浜嗚В锛�
(1) 鑴傞娑堝寲鍚告敹鍜岃綁閬�锛�
(2) 閰珨鐨勫悎鎴愬拰鍒╃敤浠ュ強閰珨鐢熸垚鐨勭敓鐞嗘剰缇╋紱
(3) 椋藉拰鑴傞吀鐨勭⒊閺堝欢闀烽€斿緫鑴傝偑閰哥⒊閺堢殑寤朵几鍙婁笉椋藉拰鑴傝偑閰哥殑鍚堟垚锛�
(4) 澶氫笉椋藉拰鑴傝偑閰哥殑閲嶈琛嶇敓鐗�锛岃剛鑲吀鍚堟垚鐨勮绡€(ji茅)锛�
鎺屾彙锛�
(1) 鑴傝泲鐧借垏杓夎剛铔嬬櫧锛�
(2) 鑴傝偑姘磋В鐨勯檺閫熼叾锛�
(3) 鐢樻补鍜岃剛鑲吀鐨勫垎瑙ｄ唬璎濆強鑳介噺鐢�(ch菐n)鐢熸儏娉併€�
(4) 閰珨鐨勬蹇�锛�
(5) 鑴傝偑閰稿垎瑙ｄ唬璎濈殑瑾跨瘈(ji茅)锛�
(6) 鍗佸叚纰充互涓嬮＝鍜岃剛鑲吀鐨勫悎鎴愰亷绋嬩互鍙婇檺閫熼叾锛�
(7) 鑴傝偑閰稿悎閰跺京鍚堥珨鐨勭祫鎴�锛�
(8) L-伪 -纾烽吀鐢樻补鐨勫悎鎴�銆佽剛鑲殑鍚堟垚锛�
(9) 纾疯剛鍜岃喗鍥洪唶浠ｈ瑵锛�
锛堝叓锛夋皑鍩洪吀鍜屾牳鑻烽吀鐨勪唬璎�
1锛庤€冩牳鐭ヨ瓨榛�
(1) 杞夋皑鍩轰綔鐢ㄥ強杞夋皑閰剁殑鐗归粸锛涜泲鐧介叾楂旈伕鎿囬檷瑙ｆ硾绱犲寲鐨勮泲鐧借唱锛�
(2) 鑴皑鍩轰綔鐢紱灏跨礌鐨勫舰鎴愰亷绋嬪強褰㈡垚閮ㄤ綅鍙婂翱绱犲惊鐠�(hu谩n)鑸� TCA 寰挵(hu谩n)鐨勮伅(li谩n)绯�锛�
(3) ?-閰吀鐨勪唬璎�(纰抽鏋剁殑姘у寲)锛涚敓绯栨皑鍩洪吀鑸囩敓閰皑鍩洪吀锛�
(4) 鑻笝姘ㄩ吀鍜岄叒姘ㄩ吀浠ｈ瑵锛涙皑鍩洪吀鑸囩敓鐗╂椿鎬х墿璩紱姘ㄥ熀閰歌垏涓€纰冲柈浣�锛�
(5) 鍢屽懁鏍歌嫹閰哥殑鍒嗚В浠ｈ瑵锛涘槯鍟舵牳鑻烽吀鐨勫垎瑙ｄ唬璎�锛�
(6) 鍢屽懁鏍歌嫹閰稿拰鍢у暥鏍歌嫹閰稿緸闋悎鎴�锛涜劔姘ф牳鑻烽吀鐨勫悎鎴�
2锛� 鍩烘湰瑕佹眰
浜嗚В锛�
(1)鑳炲収铔嬬櫧璩檷瑙ｇ殑鏂瑰紡锛�
(2)姗熼珨灏嶈泲鐧借唱鐨勬秷鍖栧惛鏀�锛�
(3)澶栨簮铔嬬櫧璩拰鍏ф簮铔嬬櫧璩殑闄嶈В锛�
(4)姘ㄥ熀閰歌垏鐢熺墿娲绘€х墿璩�锛�
(5)鑳虹殑浠ｈ瑵鍜� CO2 鐨勫幓璺紱
(6)鍚～姘ㄥ熀閰哥殑浠ｈ瑵锛屽悇绋皑鍩洪吀鐨勫悎鎴�锛屾皑鍩洪吀閲嶈琛嶇敓鐗╃殑鐢熺墿鍚堟垚锛�
(7)鏍歌嫹閰搁叾鍜屾牳鑻烽叾锛�
(8)鏍歌嫹閰哥敓鐗╁悎鎴愮殑鍩烘湰閫斿緫锛涜紨閰舵牳鑻烽吀鐨勭敓鐗╁悎鎴�;
鎺屾彙锛�
(1)杞夋皑鍩轰綔鐢ㄥ強杞夋皑閰剁殑鐗归粸锛�
(2)姘ㄥ熀閰哥殑杞夋皑鍩轰綔鐢ㄥ拰鑱�(li谩n)鍚堣劔姘ㄥ熀浣滅敤锛�
(3)铔嬬櫧閰堕珨閬告搰闄嶈В娉涚礌鍖栫殑铔嬬櫧璩紱
(4)?-閰吀鐨勪唬璎�(纰抽鏋剁殑姘у寲)锛涚敓绯栨皑鍩洪吀鑸囩敓閰皑鍩洪吀
(5)鑻笝姘ㄩ吀鍜岄叒姘ㄩ吀浠ｈ瑵锛屾皑鍩洪吀鑸囩敓鐗╂椿鎬х墿璩紝姘ㄥ熀閰歌垏涓€纰冲柈浣�锛�
(6)鍢屽懁鏍歌嫹閰哥殑鍒嗚В鍜屽槯鍟舵牳鑻烽吀鐨勫垎瑙ｄ唬璎濈敘(ch菐n)鐗╋紱
(7)闄愬埗鎬ф牳閰稿収鍒囬叾鐨勬蹇�锛�
(8)鍢屽懁鏍歌嫹閰稿拰鍢у暥鏍歌嫹閰稿緸闋悎鎴愶紱
(9)鏍歌嫹閰歌綁鍖栨垚鏍歌嫹涓夌７閰稿強鑴哀鏍歌嫹閰哥殑鍚堟垚锛�
(10) 灏跨礌鐨勫舰鎴愰亷绋嬪強褰㈡垚閮ㄤ綅鍙婂翱绱犲惊鐠�(hu谩n)鑸� TCA 寰挵(hu谩n)鐨勮伅(li谩n)绯�锛�
锛堜節锛夋牳閰稿拰铔嬬櫧璩殑鐢熺墿鍚堟垚
1锛庤€冩牳鐭ヨ瓨榛�
(1)DNA 寰╁埗閬庣▼锛汥NA 鐨勫崐涓嶉€ｇ簩(x霉)寰╁埗鍜� DNA 寰╁埗楂旂郴锛涢€嗚綁閷勯叾 鍦� DNA 鎸囧皫涓�
鐨� RNA 鍚堟垚閬庣▼锛涚湡鏍哥敓鐗╀腑 RNA 鐨勫姞宸�锛�
(2)铔嬬櫧璩悎鎴愮殑鍒嗗瓙鍩虹鍙婂悇鐗╄唱鐨勪綔鐢�锛涢伜鍌冲瘑纰肩殑鍩烘湰鐗规€�锛�
2锛� 鍩烘湰瑕佹眰
浜嗚В锛�
(1) RNA 鐨勯€嗚綁閷勯亷绋�锛涜│绠″収 cDNA 鐨勫悎鎴�;
(2)鍘熸牳鐢熺墿涓� RNA 鐨勫姞宸�锛�
鎺屾彙锛�
(1)DNA 寰╁埗閬庣▼锛汥NA 鐨勫崐涓嶉€ｇ簩(x霉)寰╁埗鍜� DNA 寰╁埗楂旂郴锛汥NA 寰╁埗鐨勫繝瀵︽€�锛�
(2)閫嗚綁閷勯叾鐨勭壒榛�锛�
(3)鍦� DNA 鎸囧皫涓嬬殑 RNA 鍚堟垚閬庣▼鍙婃墍鍙冭垏鐨勬垚鍒�锛�
(4)鐪熸牳鐢熺墿涓� RNA 鐨勫姞宸�锛�
(5)铔嬬櫧璩悎鎴愮殑鍒嗗瓙鍩虹鍙婂悇鐗╄唱鐨勪綔鐢�锛�
(6)閬哄偝瀵嗙⒓鐨勫熀鏈壒鎬�锛�
浜�銆佸弮鑰冩浉鐩�
銆婄敓鐗╁寲瀛搞€嬶紙绗� 3 鐗堬級锛岀帇閺″窎锛岄珮绛夋暀鑲插嚭鐗堢ぞ锛�2002銆�
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鏂囩珷鎼滅储
鎮ㄧ従(xi脿n)鍦ㄧ殑浣嶇疆锛� 
