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鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�(gu贸)400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ(xu茅)鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗�锛岄儴鍒嗗(xu茅)鏍℃洿鏂拌嚦2012骞�锛�2013骞�锛涘潎鎻愪緵鏀惰不(f猫i)涓嬭級銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛�(di菐n)鎿娾€�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛�(di菐n)鎿娾€�鑰冨崥瑭﹀嵎搴�(k霉)鈥� 涓嬭級
銆婂垎瀛愮敓鐗╁(xu茅)銆嬭绋嬭€冭│澶х侗
涓€銆� 鍙冭€冩浉鐩�
1. 銆婄従(xi脿n)浠ｅ垎瀛愮敓鐗╁(xu茅)銆嬮珮绛夋暀鑲插嚭鐗堢ぞ锛堢鍥涚増锛�.鏈辩帀璩�.2013 骞�
2.銆奍nstant Notes in Molecular Biology銆� ISBN 7-03-007305-3/Q.847
BIOS Science Publisher ,UK 1997, 绉戝(xu茅)鍑虹増绀� 2000 骞�
浜�銆� 鑰冭│鍏�(n猫i)瀹硅垏鍩烘湰瑕佹眰
绗竴绔� 绱�(x矛)鑳炶垏澶у垎瀛�
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鏈珷瑕佹眰鑰冪敓浜嗚В绱�(x矛)鑳炶垏澶у垎瀛愮殑鍩烘湰姒傚康銆�
[鑰冭│鍏�(n猫i)瀹筣
1 绱�(x矛)鑳炲垎椤�
2 绱�(x矛)鑳炲櫒
3 澶у垎瀛�
4 澶у垎瀛愬寘瑁�
绗簩绔� 铔嬬櫧绲�(ji茅)妲�(g貌u)
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鏈珷瑕佹眰鑰冪敓浜嗚В姘ㄥ熀閰歌垏铔嬬櫧璩�(zh矛)鐨勫熀鏈蹇�锛屾帉鎻¤泲鐧借唱(zh矛)鐨勭祼(ji茅)妲�(g貌u)鑸囧姛鑳姐€�
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1 姘ㄥ熀閰�
2 铔嬬櫧绲�(ji茅)妲�(g貌u)鑸囧姛鑳�
3 铔嬬櫧鍒嗘瀽鎶€琛�(sh霉)
绗笁绔� 鏍搁吀鎬ц唱(zh矛)
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鏈珷瑕佹眰鑰冪敓浜嗚В鏍搁吀鐨勫厜璀滃拰鐔辨€ц唱(zh矛)鍙� DNA 瓒呰灪鏃�锛屾帉鎻℃牳閰哥殑绲�(ji茅)妲�(g貌u)鍙婂叾鐞嗗寲鎬ц唱(zh矛)銆�
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3 鏍搁吀鐨勫厜璀滃拰鐔辨€ц唱(zh矛)
4 DNA 瓒呰灪鏃�
绗洓绔� 鍘熸牳鍜岀湡鏍哥殑鏌撹壊楂旂祼(ji茅)妲�(g貌u)
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鏈珷瑕佹眰鑰冪敓浜嗚В鍘熸牳鐨勬煋鑹查珨绲�(ji茅)妲�(g貌u)鍙婂熀鍥犵祫鐨勫京(f霉)闆滄€�锛屾帉鎻℃煋鑹查珨绲�(ji茅)妲�(g貌u)鍙婄湡鏍告煋鑹查珨
绲�(ji茅)妲�(g貌u)鐨勫熀鏈壒寰併€�
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1 鍘熸牳鐨勬煋鑹查珨绲�(ji茅)妲�(g貌u)
2 鏌撹壊璩�(zh矛)绲�(ji茅)妲�(g貌u)
3 鐪熸牳鏌撹壊楂旂祼(ji茅)妲�(g貌u)
4 鍩哄洜绲勭殑寰�(f霉)闆滄€�
绗簲绔� DNA 寰�(f霉)鍒�
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鏈珷瑕佹眰鑰冪敓鎺屾彙 DNA 寰�(f霉)鍒剁殑鍩烘湰姒傚康鍜屾(j墨)鍒�銆�
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1 DNA 寰�(f霉)鍒舵瑕�
2 绱�(x矛)鑿� DNA 寰�(f霉)鍒�
3 鐪熸牳 DNA 寰�(f霉)鍒�
绗叚绔� DNA 鎼嶅偡銆佷慨寰�(f霉)鍜岄噸绲�
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鏈珷瑕佹眰鑰冪敓鎺屾彙 DNA 绐佽畩锛屾悕鍌风殑鍩烘湰姒傚康鍙婇鍨嬶紝浜嗚В DNA 淇京(f霉)鍙婇噸绲勩€�
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1 绐佽畩
2 DNA 鎼嶅偡
3 DNA 淇京(f霉)
4 閲嶇祫
绗竷绔� 鍩哄洜鎿嶄綔
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鏈珷瑕佹眰鑰冪敓浜嗚В鍩哄洜鎿嶄綔鍩烘湰姒傚康銆佸師鐞嗗拰鎶€琛�(sh霉)銆�
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1 DNA 鍏嬮殕姒傝
2 璩�(zh矛)绮掔殑鍒跺倷
3 闄愬埗鎬у収(n猫i)鍒囬叾鍜岄浕娉�
4 閫ｆ帴銆佽綁(zhu菐n)鍖栧拰閲嶇祫瀛愮殑鍒嗘瀽
绗叓绔� 鍏嬮殕杓夐珨
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鏈珷瑕佹眰鑰冪敓浜嗚В鐩墠鍒嗗瓙鐢熺墿瀛�(xu茅)鏂归潰甯哥敤鐨勫咕澶ч鍏嬮殕杓夐珨銆�
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1 瑷�(sh猫)瑷�(j矛)璩�(zh矛)绮掕級楂�
2 鍣弻楂旇級楂�
3 绮樼璩�(zh矛)绮掑拰閰垫瘝浜哄伐鏌撹壊楂旓紙YACs锛�
4 鐪熸牳杓夐珨
绗節绔� 鍩哄洜鏂囧韩(k霉)鍜岀閬�
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鏈珷瑕佹眰鑰冪敓浜嗚В鍩哄洜鏂囧韩(k霉)鐨勬湁闂�(gu膩n)鍩烘湰姒傚康锛屼簡瑙ｅ缓绔� cDNA 鏂囧韩(k霉)鐨勫熀鏈亷绋嬪拰绡╅伕鏂�
搴�(k霉)鐨勬椹�銆�
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1 鍩哄洜鏂囧韩(k霉)
2 cDNA 鏂囧韩(k霉)
3 绡╅伕姝ラ
绗崄绔� 鍒嗘瀽鍜屼娇鐢ㄥ厠闅嗙殑 DNA
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鏈珷瑕佹眰鑰冪敓浜嗚В鍏嬮殕鐨勫悗绾�(x霉)鍒嗘瀽鎶€琛�(sh霉)锛屽苟灏�(du矛)鍏嬮殕鎶€琛�(sh霉)鍦ㄩ啱(y墨)瀛�(xu茅)绛夋柟闈㈢殑瀵�(sh铆)闅涙噳(y墨ng)鐢ㄦ湁鍒濇
瑾�(r猫n)璀�(sh铆)銆�
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1 鍒嗘瀽鍏嬮殕
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3 PCR
4 鍏嬮殕鍩哄洜鐨勭祼(ji茅)妲�(g貌u)鍒嗘瀽
5 鍏嬮殕鍩哄洜鐨勭獊璁�
6 鍏嬮殕鎶€琛�(sh霉)鐨勬噳(y墨ng)鐢�
绗崄涓€绔� 鍘熸牳杞�(zhu菐n)閷�
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鏈珷瑕佹眰鑰冪敓鎺屾彙杞�(zhu菐n)閷勭殑鍩烘湰姒傚康鍜屽師鏍歌綁(zhu菐n)閷勭殑涓昏鍙冭垏鑰咃紙RNA 鑱氬悎閰跺拰鍟熷嫊(d貌ng)瀛愶級
浠ュ強鍘熸牳杞�(zhu菐n)閷勭殑閬庣▼锛堣捣濮�銆佸欢浼稿拰绲傛锛夈€�
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1 杞�(zhu菐n)閷勭殑鍩烘湰姒傚康
2 澶ц吀妗胯弻 RNA 鑱氬悎閰�
3 澶ц吀妗胯弻?
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鍟熷嫊(d貌ng)瀛�
4 鍘熸牳杞�(zhu菐n)閷勭殑璧峰銆佸欢浼稿拰绲傛
绗崄浜岀珷 鍘熸牳杞�(zhu菐n)閷勮(di脿o)鎺�
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鏈珷瑕佹眰鑰冪敓鎺屾彙鍘熸牳杞�(zhu菐n)閷勮(di脿o)鎺х殑姗�(j墨)鍒�銆�
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1 鍗婁钩绯栨搷绺卞瓙
2 鑹茶壊姘ㄩ吀鎿嶇副瀛�
3 鍏朵粬?鍥犲瓙灏�(du矛)杞�(zhu菐n)閷勭殑瑾�(di脿o)鎺�
绗崄涓夌珷 鐪熸牳杞�(zhu菐n)閷�
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鏈珷瑕佹眰鑰冪敓鎺屾彙鐪熸牳杞�(zhu菐n)閷勭殑涓夌ó涓昏 RNA 鑱氬悎閰�銆佹墍杞�(zhu菐n)閷勭殑鍩哄洜椤炲瀷鍜屽弮鑸囪綁(zhu菐n)閷勯亷
绋嬪悇绋洜瀛愩€�
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2 RNA 鑱氬悎閰� I 鎵€杞�(zhu菐n)閷勭殑鍩哄洜锛氭牳绯栭珨 RNA 鍩哄洜閲嶅京(f霉)搴忓垪
3 鍚堥叾 III 鎵€杞�(zhu菐n)閷勭殑鍩哄洜锛�5S 鏍哥硸楂� RNA 鍜� tRNA 鐨勮綁(zhu菐n)閷�
4 鑱氬悎閰� II 鎵€杞�(zhu菐n)閷勭殑鍩哄洜锛�?ji菐n)顣�?d貌ng)瀛愬拰澧炲挤(qi谩ng)瀛�
5 閷勫洜瀛愬拰 RNA 鑱氬悎閰� II 杞�(zhu菐n)閷勫暉鍕�(d貌ng)
绗崄鍥涚珷 鐪熸牳杞�(zhu菐n)閷勮(di脿o)鎺�
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鏈珷瑕佹眰鑰冪敓鎺屾彙鐪熸牳杞�(zhu菐n)閷勮(di脿o)鎺х殑姗�(j墨)鍒�銆�
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1 鐪熸牳杞�(zhu菐n)閷勫洜瀛�
2 杞�(zhu菐n)閷勮(di脿o)鎺х殑姗�(j墨)鍒�
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