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3銆佹帉鎻￠啔鍜岀挵(hu谩n)姘у寲鍚堢墿鐨勫埗鍌欐柟娉�
4銆佹帉鎻￠啔鐨勫寲瀛�(xu茅)鍙嶆噳(y墨ng)鐗规€�
5銆佹帉鎻＄挵(hu谩n)姘т箼鐑峰湪鏈夋(j墨)鍚堟垚鍜屽伐妤�(y猫)鐢熺敘(ch菐n)涓殑鎳�(y墨ng)鐢�
绗崄涓€绔� 閱�銆侀叜鍜岄唽
1銆佹帉鎻￠啗鍜岄叜鐨勭祼(ji茅)妲�(g貌u)鍜屽懡鍚�銆�
2銆佹帉鎻￠啗銆侀叜鐨勪富瑕佸埗娉曘€�
3銆佹帉鎻￠啗銆侀叜鐨勫寲瀛�(xu茅)鎬ц唱(zh矛)鍜岃Κ鏍稿姞鎴愬弽鎳�(y墨ng)姗�(j墨)鐞�銆�
4銆佺啛鎮夐啗閰殑璀滃湒
5銆佷簡瑙ｉ唽鐨勭祼(ji茅)妲�(g貌u)鐗归粸(di菐n)鍜屽寲瀛�(xu茅)鎬ц唱(zh矛)
娴欐睙宸ユキ(y猫)澶у(xu茅)鐮旂┒鐢熷叆瀛�(xu茅)鑰冭│鑷懡椤岀鐩€冭│澶х侗
绗崄浜岀珷 缇ч吀
1銆佹帉鎻＄晶閰哥殑绲�(ji茅)妲�(g貌u)鍜屽懡鍚�
2銆佹帉鎻＄晶閰哥殑閲嶈鍒跺倷鍜屾柟娉�銆�
3銆佹帉鎻＄晶閰哥殑鍖栧(xu茅)鎬ц唱(zh矛)銆�
4銆佹帉鎻￠叝鍖栧拰姘磋В鍙嶆噳(y墨ng)姗�(j墨)鐞嗗強褰遍熆鍙嶆噳(y墨ng)娲绘€х殑鍥犵礌銆�
5銆佹帉鎻＄茎鍩洪吀鐨勫埗鍌欏拰鎬ц唱(zh矛)銆�
绗崄涓夌珷 缇ч吀琛嶇敓鐗�
1銆佹帉鎻＄晶閰歌鐢熺墿鐨勫惈缇╁拰鍛藉悕銆�
2銆佹帉鎻＄晶閰歌鐢熺墿鐨勫寲瀛�(xu茅)鎬ц唱(zh矛)銆�
绗崄鍥涚珷 尾-浜岀景鍩哄寲鍚堢墿
绗崄浜旂珷 鏈夋(j墨)鍚爱鍖栧悎鐗�
1銆佹帉鎻¤兒鐨勫垎椤炲拰鍛藉悕銆�
2銆佹帉鎻¤兒鐨勫埗鍌欐柟娉�銆�
3銆佹帉鎻¤兒鐨勪富瑕佸寲瀛�(xu茅)鍙嶆噳(y墨ng)鍜屽閵ㄥ牽鐨� Hofmann 娑堥櫎鍙嶆噳(y墨ng)銆�
4銆佹帉鎻￠噸姘菇鐨勫埗鍌�銆佸熀鏈弽鎳�(y墨ng)鍙婂叾鍦ㄦ湁姗�(j墨)鍚堟垚涓婄殑鎳�(y墨ng)鐢�銆�
5銆佹帉鎻″伓姘寲鍚堢墿鐨勫埗鍌欏拰鍋跺悎鍙嶆噳(y墨ng)姊濅欢銆�
绗崄鍏珷 鏈夋(j墨)鍚～銆佸惈纾峰拰鍚鍖栧悎鐗�
绗崄涓冪珷 闆滅挵(hu谩n)鍖栧悎鐗�
1銆佷簡瑙ｉ洔鐠�(hu谩n)鍖栧悎鐗╃殑鍒嗛鍜屽懡鍚�
2銆佹帉鎻″憢鍠�銆佸悺鍜€佸櫥鍚�銆佸悺鍟躲€佸柟鍟変簲鍊�(g猫)鍏稿瀷闆滅挵(hu谩n)鐨勭祼(ji茅)妲�(g貌u)鍙婂叾琛嶇敓鐗╃殑鍛藉悕
3銆佹帉鎻′笂杩颁簲鍊�(g猫)闆滅挵(hu谩n)鐨勫寲瀛�(xu茅)鐗瑰緛
绗崄鍏珷 绯栥€佹皑鍩洪吀銆佽泲鐧借唱(zh矛)鍜屾牳閰�
2.鍒嗘瀽鍖栧(xu茅)閮ㄥ垎鍏烽珨鍏�(n猫i)瀹癸細
2.1 姒傝珫銆� 浜嗚В鍒嗘瀽鍖栧(xu茅)浠诲嫏(w霉)銆佸垎鏋愭柟娉曠殑鍒嗛銆佹淮瀹氬垎鏋愬皪(du矛)鍖栧(xu茅)鍙嶆噳(y墨ng)鐨勮姹�銆傛帉鎻″熀婧�(zh菙n)鐗╄唱(zh矛)鍜�
妯�(bi膩o)婧�(zh菙n)婧舵恫鐨勯厤缃�锛屾帉鎻℃淮瀹氬垎鏋愯▓(j矛)绠�銆�
2.2 鍒嗘瀽瑭︽ǎ鐨勯噰闆嗚垏鍒跺倷銆備簡瑙ｈ│妯ｇ殑閲囬泦銆佽│妯ｇ殑鍒跺倷鍜岃│妯ｇ殑鍒嗚В鏂规硶銆�
2.3 鍒嗘瀽鍖栧(xu茅)涓殑瑾ゅ樊鑸囨暩(sh霉)鎿�(j霉)铏曠悊銆� 鎺屾彙鍒嗘瀽鍖栧(xu茅)涓殑瑾ゅ樊鐨勬蹇点€佸垎椤�锛岀簿瀵嗗害鍜屾簴(zh菙n)纰哄害鐨勮〃绀�銆�
鎺屾彙鏈夋晥鏁�(sh霉)瀛楀強鍏堕亱(y霉n)绠楄(gu墨)鍓�锛屼簡瑙ｅ垎鏋愬寲瀛�(xu茅)涓殑鏁�(sh霉)鎿�(j霉)铏曠悊銆佹帉鎻￠’钁楁€ф椹�(y脿n)鍜屾彁楂樺垎鏋愮祼(ji茅)鏋滄簴(zh菙n)纰哄害
鐨勬柟娉�銆�
2.4 鍒嗘瀽鍖栧(xu茅)涓殑璩�(zh矛)閲忎繚璀夎垏璩�(zh矛)閲忔帶鍒�銆� 浜嗚В鍒嗘瀽绲�(ji茅)鏋滅殑鍙潬鎬�銆佸垎鏋愭柟娉曠殑鍙潬鎬у拰鍒嗘瀽鍚庣殑
璩�(zh矛)閲忎繚璀夊拰璩�(zh矛)閲忔帶鍒�銆�
2.5 閰稿牽婊村畾娉�銆備簡瑙ｆ憾娑蹭腑鐨勯吀鍫垮弽鎳�(y墨ng)鑸囧钩琛�銆傛帉鎻￠吀鍫跨祫鍒嗙殑骞宠 婵冨害鑸囧垎甯冪郴鏁�(sh霉)锛涗簡瑙ｉ吀鍫�
鎸囩ず鍔戠殑璁婅壊鍘熺悊锛涙帉鎻￠吀鍫挎淮瀹氬師鐞嗗拰绲傞粸(di菐n)瑾ゅ樊銆備簡瑙ｉ吀鍫挎淮瀹氱殑鎳�(y墨ng)鐢�锛屼簡瑙ｉ潪姘存憾娑蹭腑鐨勯吀鍫�
婊村畾銆�
2.6 绲�(lu貌)鍚堟淮瀹氭硶銆備簡瑙ｅ垎鏋愬寲瀛�(xu茅)涓父鐢ㄧ殑绲�(lu貌)鍚堢墿锛屾帉鎻� EDTA 鐨勭壒鎬у拰绲�(lu貌)鍚堝钩琛″師鐞�锛涙帉鎻″壇鍙嶆噳(y墨ng)绯�
鏁�(sh霉)鍜屾浠剁┅(w臎n)瀹氬父鏁�(sh霉)浠ュ強绲�(lu貌)鍚堟淮瀹氬師鐞嗐€傛帉鎻￠噾灞寚绀哄姂璁婅壊鍘熺悊锛岄伕鎿囧師鍓�銆備簡瑙ｇ祩榛�(di菐n)瑾ゅ樊銆傛帉鎻�
绲�(lu貌)鍚堟淮瀹氭浠堕伕鎿囦互鍙婃噳(y墨ng)鐢�銆�
2.7 姘у寲閭勫師婊村畾娉曘€備簡瑙ｆ哀鍖栭倓鍘熷钩琛＄煡璀�(sh铆)锛屾帉鎻℃哀鍖栭倓鍘熸淮瀹氬師鐞嗭紝浜嗚В姘у寲閭勫師婊村畾鐨勯爯(y霉)铏�
鐞�锛屾帉鎻″父瑕嬬殑骞剧ó姘у寲閭勫師婊村畾娉曠殑鐭ヨ瓨(sh铆)銆�
2.8 娌夋穩婊村畾娉�銆傛帉鎻′笁绋父鐢ㄧ殑娌夋穩婊村畾娉曠殑鍘熺悊銆佹寚绀哄姂鐨勯伕鎿�銆佹淮瀹氭浠剁殑閬告搰銆�
2.9 鍚稿厜鍏夊害娉�銆備簡瑙ｇ墿璩�(zh矛)灏�(du矛)鍏夌殑閬告搰鎬у惛鏀讹紱鎺屾彙鏈椾集-姣旂埦瀹氬緥锛屼簡瑙ｅ垎鍏夊厜搴﹁▓(j矛)鐨勫熀鏈師鐞�锛�
鎺屾彙鍚稿厜鍏夊害鍒嗘瀽鍙婂叾瑾ゅ樊鎺у埗锛屼簡瑙ｅ惛鍏夊厜搴︽硶鐨勬噳(y墨ng)鐢�銆�
2.10 鍒嗘瀽鍖栧(xu茅)涓殑甯哥敤鍒嗛洟鍜屽瘜闆嗘柟娉曘€備簡瑙ｅ父鐢ㄧ殑骞剧ó鍒嗛洟鏂规硶锛屾帉鎻¤悆鍙栧垎闆㈠師鐞嗭紙鍒嗛厤瀹氬緥銆�
鍒嗛厤绯绘暩(sh霉)銆佸垎閰嶆瘮鍜岃悆鍙栫巼锛夊強鍏舵噳(y墨ng)鐢�銆傞噸榛�(di菐n)鎺屾彙甯哥敤鐨勬湁姗�(j墨)鐗╃殑鎻愬彇鏀堕泦鏂规硶锛堝寘鎷储姘忔彁鍙�銆�
閫ｇ簩(x霉)娑�/娑茶悆鍙�銆佹按钂告埃钂搁ぞ銆佸崌鑿硶绛夛級銆佹湁姗�(j墨)鐗╃殑甯歌(gu墨)鍒嗛洟鏂规硶锛堝寘鎷笡澹撹捀椁�銆侀噸绲�(ji茅)鏅剁瓑锛夈€佽杽
灞よ壊璀滃拰鏌辫壊璀滃垎闆㈠埗鍌�銆�
娴欐睙宸ユキ(y猫)澶у(xu茅)鐮旂┒鐢熷叆瀛�(xu茅)鑰冭│鑷懡椤岀鐩€冭│澶х侗
2.11 姘ｇ浉鑹茶瓬銆備簡瑙ｆ埃鐩歌壊璀滅殑鍩烘湰姒傚康锛屾帉鎻¤壊璀滃垎闆㈢殑鍩烘湰鐞嗚珫锛屾帉鎻¤壊璀滃垎闆㈢殑姊濅欢閬告搰锛�
浜嗚В骞剧ó姘ｇ浉鑹茶瓬妾㈡脯(c猫)鍣ㄧ殑鍘熺悊锛屾帉鎻″父瑕嬬殑瀹氶噺瑷�(j矛)绠楁柟娉�銆�
2.12 楂樻晥娑茬浉鑹茶瓬銆備簡瑙ｉ珮鏁堟恫鐩歌壊璀滅殑涓昏椤炲瀷鍙婂叾鍒嗛洟鍘熺悊锛屼簡瑙ｉ珮鏁堟恫鐩歌壊璀滃剙鐨勭祫鎴�銆�
2.13 绱鍙鍚告敹鍏夎瓬 浜嗚В绱鍙鍚告敹鍏夎瓬鐨勫熀鏈蹇碉紝鎺屾彙绱鍙鍚告敹鍏夎瓬鐨勫師鐞嗕互鍙婄传
澶栧厜璀滆垏鍒嗗瓙绲�(ji茅)妲�(g貌u)鐨勯棞(gu膩n)绯�锛屾帉鎻″悇椤炴湁姗�(j墨)鐗╃殑绱鍙鐗瑰緛鍚告敹锛屼簡瑙ｇ传澶栧彲瑕嬪厜璀滄硶鐨勬噳(y墨ng)鐢�銆�
2.14 绱呭鍚告敹鍏夎瓬 浜嗚В绱呭鍚告敹鍏夎瓬鐨勫熀鏈蹇�锛屾帉鎻＄磪澶栧惛鏀跺厜璀滅殑鍘熺悊浠ュ強鍚勯鍩哄湗(tu谩n)鐨勭壒寰�
鍚告敹闋荤巼鍜屽奖闊垮洜绱�锛岃兘杓冪啛绶村湴璁€鎳傜磪澶栧惛鏀跺厜璀滆瓬鍦栵紝浜嗚В绱呭鍏夎瓬鍎€鐨勫熀鏈祼(ji茅)妲�(g貌u)銆�
2.15 鏈夋(j墨)璩�(zh矛)璀� 浜嗚В鏈夋(j墨)璩�(zh矛)璀滅殑鍩烘湰鐭ヨ瓨(sh铆)锛屾帉鎻¤唱(zh矛)璀滅殑涓昏闆婚洟鏂规硶鍜屾湁姗�(j墨)璩�(zh矛)璀滅殑鍩烘湰瑁傝В鏂瑰紡锛�
浜嗚В涓昏椤炲瀷鏈夋(j墨)鐗╃殑璩�(zh矛)璀滐紝鍒濇鎺屾彙璩�(zh矛)璀滆В鏋愯榛�(di菐n)锛屼簡瑙ｆ埃鐩歌壊璀�-璩�(zh矛)璀滆伅(li谩n)鐢ㄦ妧琛�(sh霉)鍜屾恫鐩歌壊璀�-璩�(zh矛)
璀滆伅(li谩n)鐢ㄦ妧鐨勫熀鏈師鐞嗗拰鎳�(y墨ng)鐢ㄣ€�
2.16 鏍哥鍏辨尟娉㈣瓬 浜嗚В鏍哥鍏辨尟娉㈣瓬鍩烘湰鐭ヨ瓨(sh铆)锛屾帉鎻℃牳纾佸叡鎸殑鍩烘湰鍘熺悊浠ュ強鍖栧(xu茅)浣嶇Щ鍜屽奖闊垮洜
绱狅紝浜嗚В鏍哥鍏辨尟姘瓬鍜屾牳纾佸叡鎸⒊璀滅殑鎳�(y墨ng)鐢�銆�
2.17 璀滃湒缍滃悎瑙ｆ瀽 浜嗚В鏈夋(j墨)鐗╃祼(ji茅)妲�(g貌u)缍滃悎瑙ｆ瀽涓€鑸瓥鐣ワ紝鎺屾彙鏈夋(j墨)鐗╁垎瀛愬紡鍜屽畼鑳藉湗(tu谩n)纰哄畾锛岃兘杓冪啛
绶村湴璁€鎳傚悇椤炶瓬鍦�锛屾帹灏�(d菐o)鏈夋(j墨)鍖栧悎鐗╃祼(ji茅)妲�(g貌u)銆�
浜屻€佽€冭│瑕佹眰锛堝寘鎷€冭│鏅�(sh铆)闁�銆佺附鍒�銆佽€冭│鏂瑰紡銆侀鍨嬨€佸垎?j菒n)?sh霉)姣斾緥绛夛級
鑰冭│鏅�(sh铆)闁擄細180 鍒嗛悩
绺藉垎锛�150 鍒�
鑰冭│鏂瑰紡锛氶枆鍗�
椤屽瀷鍙婂垎?j菒n)?sh霉)姣斾緥: 鏄潪锛�8%锛�銆侀伕鎿囷紙12%锛夈€佸～绌猴紙30%锛�銆佺啊(ji菐n)绛旓紙20%锛�銆佽▓(j矛)绠楁垨绲�(ji茅)妲�(g貌u)鎺ㄦ脯(c猫)锛�30%锛�,
鍏朵腑鏈夋(j墨)鍖栧(xu茅)閮ㄥ垎鍗� 70%锛屽垎鏋愬寲瀛�(xu茅)閮ㄥ垎鍗� 30%銆�
涓�銆佷富瑕佸弮鑰冩浉鐩�
1.銆婃湁姗�(j墨)鍖栧(xu茅)銆嬶紙绗簲鐗堬級寮垫枃鍕や富绶� 楂樼瓑鏁欒偛鍑虹増绀� 2014锛�
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