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鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ(xu茅)鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗�锛岄儴鍒嗗(xu茅)鏍℃洿鏂拌嚦2012骞�锛�2013骞达紱鍧囨彁渚涙敹璨�(f猫i)涓嬭級銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛�(di菐n)鎿娾€�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛�(di菐n)鎿娾€�鑰冨崥瑭﹀嵎搴�鈥� 涓嬭級
1
涓湅鍦拌唱(zh矛)澶у(xu茅)鐮旂┒鐢熼櫌
纰╁＋鐮旂┒鐢熷叆瀛�(xu茅)鑰冭│銆婂垎鏋愬寲瀛�(xu茅)銆嬭€冭│澶х侗
(瀛�(xu茅)琛�(sh霉)鍨嬭垏灏堟キ(y猫)瀛�(xu茅)浣嶅瀷閫氱敤)
瑭﹀嵎绲�(ji茅)妲�(g貌u)
锛堜竴锛夊収(n猫i)瀹瑰強姣斾緥
鍒嗘瀽鍖栧(xu茅)閮ㄥ垎 绱� 88%
鍎€鍣ㄥ垎鏋愰儴鍒� 绱� 12%
锛堜簩锛夐鍨嬪強姣斾緥
绨＄瓟椤� 绱� 40%
瑷堢畻椤� 绱� 40%
瀵﹂鏂规瑷�(sh猫)瑷堥 绱� 20%
涓€銆佸垎鏋愬寲瀛�(xu茅)姒傝珫
鑰冭│鍏�(n猫i)瀹癸細
鍒嗘瀽鍖栧(xu茅)鐨勪换鍕�(w霉)鍜屼綔鐢�锛屽垎鏋愭柟娉曠殑鍒嗛鑸囧垎鏋愬寲瀛�(xu茅)鏂规硶鐨勯伕鎿�锛屽垎鏋愬寲瀛�(xu茅)鐨勭櫦(f膩)灞曠啊鍙茶垏鐧�(f膩)
灞曡定鍕�锛涘垎鏋愭脯瑭︾殑鍏ㄩ亷绋嬪強鍒嗘瀽绲�(ji茅)鏋滅殑琛ㄧず锛涙淮瀹氬垎鏋愮殑鐗归粸(di菐n)锛屾淮瀹氬垎鏋愬皪鍖栧(xu茅)鍙嶆噳(y墨ng)鐨勮姹�锛�
婊村畾鍒嗘瀽鐨勬柟寮忥紱鍩烘簴(zh菙n)鐗╄唱(zh矛)銆佹(bi膩o)婧�(zh菙n)婧舵恫鐨勯厤鍒讹紝婵冨害鐨勮〃绀哄舰寮忓強鐩镐簰鐨勬彌绠�锛屾淮瀹氬垎鏋愪腑寰呮脯
绲勫垎鍚噺鐨勮▓绠�銆�
鑰冭│瑕佹眰锛�
1. 浜嗚В鍒嗘瀽鍖栧(xu茅)鐨勪换鍕�(w霉)銆佷綔鐢ㄥ強鍒嗘瀽鍖栧(xu茅)鐨勭櫦(f膩)灞曡定鍕�锛岃獚(r猫n)璀樺垎鏋愭脯瑭︾殑鍏ㄩ亷绋嬪強鍒嗘瀽绲�(ji茅)鏋�
鐨勮〃绀�銆傛帉鎻″垎鏋愮祼(ji茅)鏋滅殑琛ㄩ仈(d谩)鏂瑰紡鍙婃纰鸿▓绠楀垎鏋愮祼(ji茅)鏋�銆�
2. 浜嗚В鍩烘簴(zh菙n)鐗╄唱(zh矛)銆佹(bi膩o)婧�(zh菙n)婧舵恫绛夋蹇碉紝鎺屾彙妯�(bi膩o)婧�(zh菙n)婧舵恫閰嶅埗鏂规硶銆佹績搴︾殑琛ㄧず褰㈠紡鍙婄浉浜掔殑鎻�
绠楋紝鎺屾彙婊村畾鍒嗘瀽涓淮瀹氬姂鑸囪婊村畾鐗╃殑瑷堥噺闂�(gu膩n)绯诲強鏈夐棞(gu膩n)瑷堢畻銆�
浜�銆佸垎鏋愯│妯ｇ殑閲囬泦鑸囧埗鍌�
鑰冭│鍏�(n猫i)瀹癸細
鍒嗘瀽瑭︽ǎ閲囬泦鐨勪綔鐢ㄨ垏鏂规硶锛屽浐楂旇│妯ｇ殑鍒跺倷閬庣▼鍙婄府鍒嗗叕寮忕殑鎳�(y墨ng)鐢�锛涜│妯ｇ殑鍒嗚В鏂规硶鍙婅
姹�銆�
鑰冭│瑕佹眰锛�
1. 浜嗚В瑭︽ǎ鐨勯噰闆嗗湪鍒嗘瀽娓│宸ヤ綔涓殑閲嶈浣滅敤锛屾帉鎻¤│妯ｉ噰闆嗙殑鏂规硶鑸囧伐浣滃師鍓�銆�
2. 鎺屾彙鍥洪珨瑭︽ǎ鐨勫埗鍌欓亷绋嬪強绺垎鍏紡鐨勬噳(y墨ng)鐢ㄣ€�
3. 鎺屾彙鍒嗚В瑭︽ǎ鐨勫熀鏈柟娉曞強宸ヤ綔鍘熷墖銆�
涓�銆佸垎鏋愬寲瀛�(xu茅)涓殑瑾ゅ樊鑸囨暩(sh霉)鎿�(j霉)铏曠悊
鑰冭│鍏�(n猫i)瀹癸細
瑾ゅ樊鐨勭ó椤炲強鐗归粸(di菐n)銆佽宸締婧愬強娓涘皬瑾ゅ樊鐨勬柟娉�锛屾簴(zh菙n)纰哄害鍙婄簿瀵嗗害鐨勫熀鏈蹇�锛屽悇绋宸強
2
鍋忓樊鐨勮▓绠楋紱鏈夋晥鏁�(sh霉)瀛楃殑姒傚康鍙婃湁鏁堟暩(sh霉)瀛楃殑淇磩瑕�(gu墨)鍓囧拰閬�(y霉n)绠楄(gu墨)鍓�锛涚附楂斿拰妯ｆ湰鐨勭当(t菕ng)瑷堝(xu茅)姒傚康锛岄毃
姗�(j墨)瑾ゅ樊鐨勬鎱�(t脿i)鍒嗗竷鐨勭壒榛�(di菐n)鍙婂崁(q奴)闁撴鐜囩殑瑷堢畻锛� t 鍒嗗竷鐨勭壒榛�(di菐n)銆佺附楂斿钩鍧囧€肩殑浼拌▓锛� t 妾㈤娉曞拰 F
妾㈤娉曞強鍏堕亱(y霉n)鐢�锛涘彲鐤戝€肩殑鍙栬垗鏂规硶锛涚郴绲�(t菕ng)瑾ゅ樊銆侀毃姗�(j墨)瑾ゅ樊鍙婃サ鍊艰宸殑鍌抽仦銆�
鑰冭│瑕佹眰锛�
1. 浜嗚В瑾ゅ樊鑸囧亸宸殑姒傚康锛屼簡瑙ｆ簴(zh菙n)纰哄害鍙婄簿瀵嗗害鐨勬蹇�锛屾帉鎻″悇绋宸強鍋忓樊鐨勮▓绠�銆�
2. 鍒ゆ柗瑾ゅ樊鐨勭ó椤炲強鍒嗘瀽瑾ゅ樊鐨勪締婧�锛屾帉鎻℃彁楂樺垎鏋愮祼(ji茅)鏋滄簴(zh菙n)纰哄害鍙婄簿瀵嗗害鐨勬柟娉曞強鎺柦銆�
3. 浜嗚В鏈夋晥鏁�(sh霉)瀛楃殑淇磩鑸囬亱(y霉n)绠楄(gu墨)鍓�锛屾纰鸿〃閬�(d谩)瀵﹂鏁�(sh霉)鎿�(j霉)銆�
4. 浜嗚В闅ㄦ(j墨)瑾ゅ樊鐨勬鎱�(t脿i)鍒嗗竷鐗归粸(di菐n)锛屾帉鎻″崁(q奴)闁撴鐜囩殑鐩搁棞(gu膩n)瑷堢畻锛涗簡瑙� t 鍒嗗竷鐨勭壒榛�(di菐n)锛屾帉鎻＄附楂�
骞冲潎鍊肩殑瀛樺湪鍗€(q奴)闁撹垏缃俊搴︾殑鐩搁棞(gu膩n)瑷堢畻銆�
5. 鎺屾彙鍒嗘瀽鍖栧(xu茅)涓父鐢ㄧ殑椤憲鎬ф椹楁柟娉�(t 妾㈤娉曞拰 F 妾㈤娉�)銆�
6. 鎺屾彙 4d 娉�銆丟rubbs 娉曞拰 Q 妾㈤娉曢€�(j矛n)琛屽彲鐤戝€肩殑鍙栬垗銆�
7. 浜嗚В绯荤当(t菕ng)瑾ゅ樊銆侀毃姗�(j墨)瑾ゅ樊鍙婃サ鍊艰宸殑鍌抽仦瑕�(gu墨)寰�銆�
8. 鍒濇瀛�(xu茅)鏈冪敤瑾ゅ樊鐞嗚珫鎸囧皫(d菐o)鍜岃ō(sh猫)瑷堝椹楁柟妗堛€�
鍥�銆侀吀鍫挎淮瀹氭硶
鑰冭│鍏�(n猫i)瀹癸細
娲诲害鍜屾椿搴︾郴鏁�(sh霉)鐨勬蹇�锛岄吀鍫胯唱(zh矛)瀛愮悊璜栬垏閰稿牽鐨勮В闆㈠钩琛�锛岃唱(zh矛)瀛愭柟绋嬪紡锛涘垎甯冨垎?j菒n)?sh霉)鐨勬蹇靛強
瑷堢畻锛宲H 鍊煎皪婧舵恫涓悇瀛樺湪褰㈠紡鐨勫奖闊�锛涙憾娑蹭腑 H
+
婵冨害鐨勬湁闂�(gu膩n)瑷堢畻锛涚珐娌栨憾娑茬殑鎬ц唱(zh矛)銆佺祫鎴愪互
鍙� pH 鍊肩殑瑷堢畻锛岀珐娌栧閲忕殑姒傚康鍙婂奖闊跨珐娌栧閲忕殑鍥犵礌锛涢吀鍫挎寚绀哄姂鐨勮畩鑹插師鐞�銆佽畩鑹茶寖鍦嶅強
鎸囩ず鍔戠殑閬告搰鍘熷墖锛涢吀鍫挎淮瀹氶亷绋嬩腑 pH 鍊肩殑瑷堢畻锛屾淮瀹氭洸绶氱殑绻埗銆佹淮瀹氱獊韬嶅強褰遍熆婊村畾绐佽簫
鐨勫洜绱犮€傜祩榛�(di菐n)瑾ゅ樊鐨勬蹇靛強瑷堢畻锛岄吀鍫挎淮瀹氱殑鏂瑰紡鍙婇吀鍫挎淮瀹氭硶鐨勬噳(y墨ng)鐢ㄣ€�
鑰冭│瑕佹眰锛�
1. 浜嗚В娲诲害鍜屾椿搴︾郴鏁�(sh霉)鐨勬蹇�锛屾帉鎻＄浉闂�(gu膩n)鐨勮▓绠�銆�
2. 姝ｇ⒑瀵嚭婧舵恫鐨勮唱(zh矛)瀛愬钩琛″紡銆�
3. 浜嗚В鍒嗗竷鍒�?j菒n)?sh霉)鐨勬蹇点€佷綔鐢ㄥ苟鎺屾彙鐩搁棞(gu膩n)瑷堢畻銆�
4. 鎺屾彙涓€鍏冨急閰�(鍫�)婧舵恫銆佸鍏冨急閰�(鍫�)婧舵恫銆佸急閰�(鍫�)娣峰悎婧舵恫銆佸叐鎬х墿璩�(zh矛)婧舵恫鐨� pH 鍊肩殑
瑷堢畻銆�
5. 鎺屾彙绶╂矕婧舵恫鐨勪綔鐢ㄣ€佺壒鎬�銆佺祫鎴愪互鍙� pH 鍊肩殑瑷堢畻銆�
6. 鎺屾彙閰稿牽婊村畾鍘熺悊銆侀吀鍫挎淮瀹氶亷绋嬩腑 pH 鍊肩殑瑷堢畻锛屽垎鏋愬奖闊挎淮瀹氱獊韬嶇殑鍥犵礌锛屾纰洪伕鎿�
鎸囩ず鍔�锛屾帉鎻￠吀鍫挎淮瀹氱祩榛�(di菐n)瑾ゅ樊鐨勮▓绠�锛屼簡瑙ｉ吀鍫挎淮瀹氭硶鐨勫叿楂旀噳(y墨ng)鐢�銆�
7. 鑳借ō(sh猫)瑷堝父瑕嬮吀銆佸牽鐨勬淮瀹氬垎鏋愭柟妗�銆�
浜�銆佺怠(lu貌)鍚堟淮瀹氭硶
鑰冭│鍏�(n猫i)瀹癸細
鍒嗘瀽鍖栧(xu茅)涓父鐢ㄧ殑绲�(lu貌)鍚堝姂鐨勯鍨�锛屾皑缇х怠(lu貌)鍚堝姂鐨勭壒榛�(di菐n)鍙� EDTA 鑸囬噾灞洟瀛愮怠(lu貌)鍚堢墿鐨勭壒榛�(di菐n)锛�
绲�(lu貌)鍚堝弽鎳�(y墨ng)绌�(w臎n)瀹氬父鏁�(sh霉)銆佸悇绱氱怠(lu貌)鍚堢墿鐨勫垎甯�锛涚怠(lu貌)鍚堝钩琛′腑鐨勫壇鍙嶆噳(y墨ng)绯绘暩(sh霉)鍜屾浠剁┅(w臎n)瀹氬父鏁�(sh霉)鐨勬蹇靛強瑷堢畻锛�
閲戝爆闆㈠瓙鎸囩ず鍔戠殑浣滅敤鍘熺悊鍙婇伕鎿囧師鍓囷紱绲�(lu貌)鍚堟淮瀹氭硶鐨勫熀鏈師鐞�锛屽奖闊挎淮瀹氱獊韬嶇殑鍥犵礌锛岀怠(lu貌)鍚堟淮
瀹氱祩榛�(di菐n)瑾ゅ樊鐨勮▓绠�锛岀怠(lu貌)鍚堟淮瀹氫腑閰稿害鐨勬帶鍒讹紝鎻愰珮绲�(lu貌)鍚堟淮瀹氶伕鎿囨€х殑閫斿緫锛岀怠(lu貌)鍚堟淮瀹氱殑鏂瑰紡鍙婂叾
鎳�(y墨ng)鐢�銆�
鑰冭│瑕佹眰锛�
3
1. 浜嗚В鍒嗘瀽鍖栧(xu茅)涓父鐢ㄧ殑绲�(lu貌)鍚堝姂鐨勯鍨嬶紝浜嗚В姘ㄧ晶绲�(lu貌)鍚堝姂鐨勭祫鎴愮壒榛�(di菐n)鍙� EDTA 鑸囬噾灞怠(lu貌)鍚�
鐗╃殑鐗规€�銆�
2. 浜嗚В绌�(w臎n)瀹氬父鏁�(sh霉)鑸囩疮绌嶇┅(w臎n)瀹氬父鏁�(sh霉)鐨勯棞(gu膩n)绯�锛屾帉鎻″悇绱氱怠(lu貌)鍚堢墿鐨勫垎甯冭(gu墨)寰�銆�
3. 鐔熺反鎺屾彙绲�(lu貌)鍚堝钩琛′腑鐨勫壇鍙嶆噳(y墨ng)绯绘暩(sh霉)鍜屾浠剁┅(w臎n)瀹氬父鏁�(sh霉)鐨勬蹇佃垏瑷堢畻銆�
4. 浜嗚В閲戝爆闆㈠瓙鎸囩ず鍔戠殑浣滅敤鍘熺悊鍙婇伕鎿囧師鍓�锛屾帉鎻＄怠(lu貌)鍚堟淮瀹氭硶鐨勫熀鏈師鐞嗗拰婊村畾閬庣▼閲�
灞洟瀛愭績搴︾殑瑷堢畻銆傚垎鏋愬奖闊挎淮瀹氱獊韬嶇殑鍥犵礌锛屾帉鎻＄怠(lu貌)鍚堟淮瀹氱祩榛�(di菐n)瑾ゅ樊鐨勮▓绠�銆�
5. 浣跨敤婧�(zh菙n)纰烘淮瀹氱殑鍒ゅ垾寮忓垽鏂锋淮瀹氱殑鍙兘鎬�锛� 姝ｇ⒑鎺у埗婊村畾鐨勯吀搴﹁寖鍦�锛屾帉鎻℃彁楂樼怠(lu貌)鍚堟淮
瀹氶伕鎿囨€х殑閫斿緫銆�
6. 浜嗚В绲�(lu貌)鍚堟淮瀹氱殑鏂瑰紡鍙婂叾鎳�(y墨ng)鐢�锛屾帉鎻″垎鏋愮祼(ji茅)鏋滆▓绠楁柟娉曘€�
7. 鑳借ō(sh猫)瑷堢怠(lu貌)鍚堟淮瀹氬垎鏋愭柟妗�銆�
鍏�銆佹哀鍖栭倓鍘熸淮瀹氭硶
鑰冭│鍏�(n猫i)瀹癸細
妯�(bi膩o)婧�(zh菙n)闆绘サ闆诲嫝鍙婃浠堕浕妤甸浕鍕㈢殑姒傚康锛岄浕妤甸浕鍕㈠強姊濅欢闆绘サ闆诲嫝鐨勮▓绠楋紝姘у寲閭勫師鍙嶆噳(y墨ng)鐨勫钩
琛″父鏁�(sh霉)锛� 姘у寲閭勫師婊村畾鎸囩ず鍔戠殑绋鍙婁綔鐢ㄥ師鐞嗭紝姘у寲閭勫師婊村畾閬庣▼婧舵恫闆诲嫝鐨勮▓绠�锛屾淮瀹氭洸
绶氱殑绻埗锛涙哀鍖栭倓鍘熸淮瀹氶爯(y霉)铏曠悊鐨勭洰鐨�銆佽姹傝垏鏂规硶锛涙哀鍖栭倓鍘熸淮瀹氭硶鐨勫叿楂旀噳(y墨ng)鐢ㄥ強鍒嗘瀽绲�(ji茅)鏋滅殑
姝ｇ⒑瑷堢畻銆�
鑰冭│瑕佹眰锛�
1. 鎺屾彙姊濅欢闆绘サ闆诲嫝鐨勬蹇靛強瑷堢畻锛屽垽鏂峰弽鎳�(y墨ng)閫�(j矛n)琛岀殑鏂瑰悜銆�
2. 鎺屾彙骞宠 甯告暩(sh霉)鐨勮▓绠楋紝鍒ゆ柗鍙嶆噳(y墨ng)閫�(j矛n)琛岀殑绋嬪害銆�
3. 浜嗚В姘у寲閭勫師婊村畾鐨勫師鐞嗭紝鎺屾彙姘у寲閭勫師婊村畾閬庣▼婧舵恫闆诲嫝鐨勮▓绠楀強婊村畾绐佽簫鑼冨湇鐨勮▓
绠�锛屾纰洪伕鎿囨淮瀹氭寚绀哄姂銆�
4. 鎺屾彙甯哥敤鐨勬哀鍖栭倓鍘熼爯(y霉)铏曠悊鍔戠殑浣跨敤姊濅欢鍙婇櫎鍘荤殑鏂规硶銆�
5. 姝ｇ⒑瑷堢畻姘у寲閭勫師婊村畾鍒嗘瀽鐨勭祼(ji茅)鏋溿€�
6. 鎺屾彙楂橀尦閰告硶銆侀噸閴婚吀閴€娉曞強纰橀噺娉曠殑涓夐鍒嗘瀽娉曠殑鍘熺悊鍙婃噳(y墨ng)鐢�銆�
涓�銆佹矇娣€婊村畾娉�
鑰冭│鍏�(n猫i)瀹癸細
鑾埦娉�銆佷經鐖惧搱寰锋硶鍜屾硶鎻�(y谩ng)鍙告硶鐨勬矇娣€婊村畾鍘熺悊鍙婃噳(y墨ng)鐢�銆�
鑰冭│瑕佹眰锛�
1. 浜嗚В鑾埦娉曘€佷經鐖惧搱寰锋硶鍜屾硶鎻�(y谩ng)鍙告硶鐨勬矇娣€婊村畾鍘熺悊銆�
2. 鎺屾彙鑾埦娉�銆佷經鐖惧搱寰锋硶鍜屾硶鎻�(y谩ng)鍙告硶鐨勬淮瀹氭浠躲€佹寚绀哄姂鐨勯伕鎿囧強鏂规硶鐨勬噳(y墨ng)鐢ㄨ寖鍦�銆�
鍏€侀噸閲忓垎鏋�
鑰冭│鍏�(n猫i)瀹癸細
閲嶉噺鍒嗘瀽娉曠殑鍘熺悊鍙婂垎椤烇紝娌夋穩閲嶉噺娉曞皪娌夋穩褰㈠紡鍜岀ū閲忓舰寮忕殑瑕佹眰锛屾彌绠楀洜绱犲強閲嶉噺鍒嗘瀽
绲�(ji茅)鏋滅殑瑷堢畻锛涙矇娣€鐨勬憾瑙ｅ害鍙婂叾褰遍熆鍥犵礌锛屾憾瑙ｅ害銆佹憾搴︾⿳鍙婃浠舵憾搴︾⿳鐨勬蹇靛強瑷堢畻锛屾矇娣€鐨�
椤炲瀷鍜屾矇娣€鐨勫舰鎴愰亷绋�锛屽奖闊挎矇娣€绱斿害鐨勪富瑕佸洜绱狅紝鏈夋(j墨)娌夋穩鍔戠殑涓昏椤炲瀷鍙婄壒榛�(di菐n)銆�
鑰冭│瑕佹眰锛�
1. 浜嗚В閲嶉噺鍒嗘瀽娉曠殑鍘熺悊锛屾帉鎻℃矇娣€閲嶉噺鍒嗘瀽娉曠祼(ji茅)鏋滅殑瑷堢畻銆�
4
2. 鎺屾彙婧惰В搴�銆佹憾搴︾⿳鍙婃浠舵憾搴︾⿳鐨勭浉闂�(gu膩n)瑷堢畻銆�
3. 浜嗚В鍚岄洟瀛愭晥鎳�(y墨ng)銆侀菇鏁堟噳(y墨ng)銆侀吀鏁堟噳(y墨ng)鍜岀怠(lu貌)鍚堟晥鎳�(y墨ng)灏嶆憾瑙ｅ害鐨勫奖闊�锛屾帉鎻′笉鍚屾浠朵笅婧惰В搴�
鐨勮▓绠楁柟娉�銆�
4. 浜嗚В褰遍熆娌夋穩绱斿害鐨勪富瑕佸洜绱犲拰鎻愰珮娌夋穩绱斿害鐨勬柟娉�銆�
5. 浜嗚В鏈夋(j墨)娌夋穩鍔戠殑涓昏椤炲瀷鍙婄壒榛�(di菐n)銆�
涔�銆佸惛鍏夊厜搴︽硶锛�
鑰冭│鍏�(n猫i)瀹癸細
鐗╄唱(zh矛)灏嶅厜鐨勯伕鎿囨€у惛鏀�锛屽厜鍚告敹鐨勫熀鏈畾寰�锛屽垎鍏夊厜搴﹁▓鐨勪富瑕侀儴浠跺強鍔熻兘锛屽惛鏀跺厜璀�锛岄’
鑹插弽鎳�(y墨ng)鍙婇’鑹插弽鎳�(y墨ng)姊濅欢锛屾脯瀹氭尝闀峰強鍙冩瘮婧舵恫閬告搰锛屾(bi膩o)婧�(zh菙n)鏇茬窔锛屽惛鍏夊厜搴﹀垎鏋愮殑瑾ゅ樊鎺у埗锛岀ず宸�
娉�銆佸娉㈤暦娉�銆佸皫(d菐o)鏁�(sh霉)娉曠殑鍘熺悊鍙婄壒榛�(di菐n)锛屽惛鍏夊厜搴︽硶鐨勬噳(y墨ng)鐢�銆�
鑰冭│瑕佹眰锛�
1. 浜嗚В鍏夌殑鐗规€у拰鍒嗗瓙鍚告敹鍏夎瓬娉曠殑鍩烘湰鐗瑰緛锛岀啛绶存帉鎻″厜鍚告敹鐨勫熀鏈畾寰�锛涜獚(r猫n)璀樺惛鍏夊厜
搴︽硶涓紩璧疯宸殑鍘熷洜銆傜悊瑙ｆ懇鐖惧惛鍏夌郴鏁�(sh霉)鐨勬剰缇╁苟鎺屾彙瑷堢畻鏂规硶銆�
2. 浜嗚В鍒嗗厜鍏夊害瑷堝剙鍣ㄧ殑妲�(g貌u)閫犺垏鍔熻兘锛涙帉鎻￠’鑹插弽鎳�(y墨ng)鍙婂叾褰遍熆鍥犵礌銆傜啛绶存帉鎻″惛鍏夊厜搴︽硶
娓噺鏂规硶鍜屾脯閲忔浠剁殑閬告搰銆傛帉鎻＄躬鍒跺惛鏀跺厜璀滃強妯�(bi膩o)婧�(zh菙n)鏇茬窔鐨勬柟娉曪紝浜嗚В瀹氭€ц垏瀹氶噺鍒嗘瀽鐨勪緷鎿�(j霉)銆�
3. 浜嗚В鍚稿厜鍏夊害娉曟脯閲忕殑瑾ゅ樊锛屾帉鎻＄ず宸硶銆佸娉㈤暦娉�銆佸皫(d菐o)鏁�(sh霉)娉曠瓑鍚稿厜鍏夊害娉曠殑鍘熺悊鍜岀壒
榛�(di菐n)銆�
4. 浜嗚В鍚稿厜鍏夊害娉曠殑鎳�(y墨ng)鐢�銆�
鍗�銆佸垎鏋愬寲瀛�(xu茅)涓父鐢ㄧ殑鍒嗛洟鍜屽瘜闆嗘柟娉�
鑰冭│鍏�(n猫i)瀹癸細
鍥炴敹鐜囩殑瀹氱京锛涙埃鎱�(t脿i)鍒嗛洟娉曘€佹矇娣€鍒嗛洟鑸囧叡娌夋穩鍒嗛洟娉�銆佽悆鍙栧垎闆㈡硶銆侀洟瀛愪氦鎻涘垎闆㈡硶銆佺礄
鑹茶瓬娉�銆佽杽灞よ壊璀滄硶鍙婇浕娉冲垎闆㈡硶绛夊垎闆㈡柟娉曠殑鍘熺悊鍙婃噳(y墨ng)鐢�锛涘垎闆㈠瘜闆嗘柊鎶€琛�(sh霉)鏂版柟娉�銆�
鑰冭│瑕佹眰锛�
1. 鎺屾彙鍥炴敹鐜囩殑瀹氱京鍙婅▓绠椼€�
2. 浜嗚В姘ｆ厠(t脿i)鍒嗛洟娉曠殑椤炲瀷鍙婂師鐞�锛屾帉鎻″父鐢ㄧ殑娌夋穩娉曡垏鍏辨矇娣€娉曠殑鍘熺悊鍙婂垎闆㈡浠剁殑閬告搰銆�
3. 鎺屾彙钀冨彇鍒嗛洟涓垎閰嶅畾寰�銆佸垎閰嶇郴鏁�(sh霉)銆佸垎閰嶆瘮銆佽悆鍙栫巼銆佸娆¤悆鍙栫巼鐨勬蹇佃垏瑷堢畻锛涗簡
瑙ｈ灟鍚堣悆鍙栧钩琛″父鏁�(sh霉)鑸囧垎閰嶆瘮鐨勯棞(gu膩n)绯�锛屾纰洪伕鎿囪悆鍙栨浠�銆�
4. 浜嗚В闆㈠瓙浜ゆ彌鍔戠殑绋浠ュ強闆㈠瓙浜ゆ彌鍒嗛洟鐨勫師鐞嗭紝浜嗚В闆㈠瓙浜ゆ彌妯硅剛鐨勮Κ鍚堝姏澶у皬鐨勮(gu墨)
寰嬪強闆㈠瓙浜ゆ彌鍒嗛洟娉曠殑鎳�(y墨ng)鐢�銆�
5. 浜嗚В钖勫堡鑹茶瓬鍙婄礄鑹茶瓬鐨勫熀鏈師鐞嗐€�
6. 浜嗚В鐝�(xi脿n)浠ｅ垎闆㈠瘜闆嗘妧琛�(sh霉)鐨勭櫦(f膩)灞�銆�
鍗佷竴 銆佸師瀛愬厜璀滃垎鏋愭硶
鑰冭│鍏�(n猫i)瀹癸細
鍘熷瓙鍏夎瓬鐨勭敘(ch菐n)鐢�銆佽瓬绶氬挤(qi谩ng)搴︺€佽嚜鍚歌垏鑷潟銆佽儗鏅殑渚嗘簮绛夊熀鏈蹇碉紱鍘熷瓙鐧�(f膩)灏勫厜璀滄硶鐨勫熀鏈�
鍘熺悊銆佺壒榛�(di菐n)鍙婂畾鎬с€佸畾閲忔柟娉�锛屽厜婧愬垎椤炲強鐗归粸(di菐n)锛屽師瀛愮櫦(f膩)灏勫厜璀滃剙鐨勫熀鏈祼(ji茅)妲�(g貌u)鍜屼富瑕侀儴浠剁殑浣滅敤
绛夈€傚師瀛愬惛鏀跺厜璀滄硶鐨勫熀鏈師鐞�銆佸嘲鍊煎惛鏀舵脯閲忔妧琛�(sh霉)瑕侀粸(di菐n)銆佸師瀛愬惛鏀跺厜璀滃剙鐨勫熀鏈祼(ji茅)妲�(g貌u)鍜屼富瑕侀儴
浠剁殑浣滅敤锛岀┖蹇冮櫚妤电噲鐨勫伐浣滃師鐞嗭紝鐏劙鍙婄煶澧ㄧ垚鍘熷瓙鍖栧櫒鐨勭壒榛�(di菐n)绛�銆傚師瀛愮啋鍏夊厜璀滄硶鐨勫熀鏈師
5
鐞嗐€佷富瑕侀鍨嬪強鐗归粸(di菐n)锛屽叡鎸啋鍏夊強闈炲叡鎸啋鍏夈€佺啋鍏夌対婊呯瓑鍩烘湰姒傚康锛屽師瀛愮啋鍏夊厜璀滃剙鐨勫熀鏈祼(ji茅)
妲�(g貌u)鍜屼富瑕侀儴浠剁殑浣滅敤绛�銆�
鑰冭│瑕佹眰锛�
1. 鐞嗚В鍘熷瓙锛堢櫦(f膩)灏�銆佸惛鏀�銆佺啋鍏夛級鍏夎瓬鐢�(ch菐n)鐢熺殑鍩烘湰鍘熺悊锛�
2. 鐔熸倝鍘熷瓙鍏夎瓬寮�(qi谩ng)搴︼紙鐧�(f膩)灏�銆佸惛鏀�銆佺啋鍏夊挤(qi谩ng)搴︼級鐨勪富瑕佸奖闊垮洜绱�锛�
3. 浜嗚В鍘熷瓙鍏夎瓬锛堢櫦(f膩)灏勩€佸惛鏀�銆佺啋鍏夛級鍒嗘瀽鍎€鍣ㄧ殑鍩烘湰绲�(ji茅)妲�(g貌u)鍙婁富瑕侀儴浠剁殑宸ヤ綔鍘熺悊鍜屼綔鐢�锛�
4. 鎺屾彙鍚勭ó鍏夋簮銆佸師瀛愬寲鍣ㄧ殑鐗归粸(di菐n)鍙婂垎鏋愭€ц兘锛�
5. 鎺屾彙鍘熷瓙锛堢櫦(f膩)灏�銆佸惛鏀�銆佺啋鍏夛級鍏夎瓬鐨勫畾鎬�銆佸畾閲忓垎鏋愭柟娉曘€�
鍗佷簩銆� 闆诲寲瀛�(xu茅)鍒嗘瀽娉�
鑰冭│鍏�(n猫i)瀹癸細
闆诲垎鏋愬寲瀛�(xu茅)鏂规硶鍩烘湰鍘熺悊鍙婂垎椤�锛岄浕妤甸浕浣�銆佸伐浣滈浕妤点€佸弮姣旈浕妤�銆佽紨鍔╅浕妤电瓑鍩烘湰琛�(sh霉)瑾炶垏
姒傚康锛涢浕浣嶅垎鏋愭柟娉曞熀鏈師鐞�锛岃啘闆讳綅鐨勭敘(ch菐n)鐢�锛岄洟瀛愰伕鎿囨€ч浕妤碉紝绺介洟瀛愬挤(qi谩ng)搴﹁(di脿o)绡€(ji茅)鍔�锛岄浕浣嶉伕鎿�
鎬х郴鏁�(sh霉)绛夊熀鏈蹇点€傛櫘閫氭サ璀滄硶鐨勫熀鏈師鐞�锛屾サ璀滄尝鐨勫舰鎴愶紝婊存睘闆绘サ锛屾摯(ku貌)鏁ｉ浕娴佹柟绋嬪強涓昏褰�
闊垮洜绱狅紝妤佃瓬鍒嗘瀽涓殑骞叉摼闆绘祦鍙婃秷闄ゆ柟娉�锛屽柈鎺冩弿妤佃瓬娉曞拰鑴堟矕妤佃瓬娉曠殑鐗归粸(di菐n)绛�锛涢浕瑙ｈ垏搴緰
鍒嗘瀽娉曞熀鏈師鐞�銆佺壒榛�(di菐n)鍙婂崁(q奴)鍒�銆�
鑰冭│瑕佹眰锛�
1. 浜嗚В闆诲垎鏋愬寲瀛�(xu茅)鐨勫熀鏈師鐞嗗拰涓昏椤炲瀷锛�
2. 鐔熸倝闆诲垎鏋愬寲瀛�(xu茅)鐨勫熀鏈(sh霉)瑾炲拰姒傚康锛�
3. 鐔熸倝閲戝爆鍩洪浕妤电殑椤炲瀷鍙婇浕妤靛弽鎳�(y墨ng)锛�
4. 鎺屾彙闆㈠瓙閬告搰鎬ч浕妤电殑鍘熺悊鍜屾噳(y墨ng)鐢ㄨ寖鍦�锛�
5. 鎺屾彙妤佃瓬娉曠殑鍩烘湰鍘熺悊锛�
6. 浜嗚В鍠巸鎻忔サ璀滄硶銆佸惊鐠�(hu谩n)浼忓畨娉�銆佽剤娌栨サ璀滄硶鐨勫熀鏈師鐞嗗強鐗归粸(di菐n)锛�
7. 鎺屾彙闆昏В鑸囧韩鍊垎鏋愭硶鍩烘湰鍘熺悊銆�
鍗佷笁銆� 鑹茶瓬鍒嗘瀽娉�
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鎮ㄧ従(xi脿n)鍦ㄧ殑浣嶇疆锛� 
