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鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�(gu贸)400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ(xu茅)鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗�锛岄儴鍒嗗(xu茅)鏍℃洿鏂拌嚦2012骞达紝2013骞�锛涘潎鎻愪緵鏀惰不(f猫i)涓嬭級銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛�(di菐n)鎿娾€�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛�(di菐n)鎿娾€�鑰冨崥瑭﹀嵎搴�(k霉)鈥� 涓嬭級
涓湅(gu贸)绉戝(xu茅)闄㈠ぇ瀛�(xu茅)纰╁＋鐮旂┒鐢熷叆瀛�(xu茅)鑰冭│
銆婂垎瀛愮敓鐗╁(xu茅)銆嬭€冭│澶х侗
涓€銆� 鑰冭│澶х侗鐨勬€ц唱(zh矛)
鍒嗗瓙鐢熺墿瀛�(xu茅)闆栫劧鏄柊鐢熷(xu茅)绉�锛屼絾鍗绘槸鍏朵粬瀛�(xu茅)绉戝绱�(x矛)鑳炲(xu茅)銆侀伜鍌冲(xu茅)銆佺敓鐞嗗(xu茅)銆�
瑙ｅ墫瀛�(xu茅)鐨勫熀绀�(ch菙)銆傚洜姝ゅ湪閲嶉粸(di菐n)鑰冨療鍒嗗瓙鐢熺墿瀛�(xu茅)鐨勫熀绀�(ch菙)鐭ヨ瓨(sh铆)鍚屾檪(sh铆)锛岃垏鍏朵粬瀛�(xu茅)绉戠殑浜ゅ弶銆�
鑱�(li谩n)绯讳篃闇€瑕佹帉鎻°€傜壒绶ㄥ(xi臎)姝よ€冭│澶х侗浣滅偤鍙冭€�锛屼娇鑰冪敓鏇磋兘鎶婃彙鑰冭│鐨勮寖鍦嶅拰瑕�
姹�銆�
浜�銆� 鑰冭│鍏�(n猫i)瀹�
1. DNA锛孯NA 鍜岄伜鍌冲瘑纰�
鑰冭│鍏�(n猫i)瀹�
鈼� DNA 寰�(f霉)鍒剁殑涓€浜涘熀鏈蹇�
鈼� 鍙冭垏 DNA 寰�(f霉)鍒剁殑閰惰垏铔嬬櫧璩�(zh矛)锛堥噸榛�(di菐n)鏄師鏍哥敓鐗╃殑 DNA 鑱氬悎閰讹級
鈼� DNA 寰�(f霉)鍒剁殑涓€鑸亷(gu貌)绋嬪強鍏惰(di脿o)鎺�
鈼� 鐪熸牳鐢熺墿鑸囧師鏍哥敓鐗� DNA 寰�(f霉)鍒剁殑鐗归粸(di菐n)
鈼� RNA 杞�(zhu菐n)閷勭殑鍩烘湰閬�(gu貌)绋�
鈼� 杞�(zhu菐n)閷勭殑涓€鑸(gu墨)寰嬪拰姗�(j墨)鍒�
鈼� RNA 鐨勭祼(ji茅)妲�(g貌u)銆佸垎椤�(l猫i)鍜屽姛鑳�
鈼� 鍙冭垏杞�(zhu菐n)閷勭殑閰跺拰铔嬬櫧璩�(zh矛)锛堥噸榛�(di菐n)鏄� RNA 鑱氬悎閰讹級
鈼� 鐪熸牳鐢熺墿鑸囧師鏍哥敓鐗╄綁(zhu菐n)閷勭殑鍩烘湰鐗瑰緛
鈼� RNA 鐨勭法杓�銆佸啀绶ㄧ⒓鍜屽寲瀛�(xu茅)淇＞
鈼� RNA 鐨勮綁(zhu菐n)閷勫悗鍔犲伐鍙婂叾鎰忕京
鈼� mRNA銆乼RNA銆乺RNA 鐨勫悗鍔犲伐
鈼� 閫嗚綁(zhu菐n)閷勭殑閬�(gu貌)绋�
鈼� 閫嗚綁(zhu菐n)閷勭梾姣掔殑鐢熸椿鍙�
鈼� RNA 鐨勫京(f霉)鍒讹細?ji菐n)鎹�?RNA 鐥呮瘨鐨� RNA 寰�(f霉)鍒�锛岄洐閺� RNA 鐥呮瘨鐨� RNA 寰�(f霉)鍒�
鈼� RNA 鍌抽仦鍔犲伐閬哄偝淇℃伅
鈼� 鏌撹壊楂旇垏 DNA
鏌撹壊楂旀杩�
鐪熸牳鐢熺墿鍩哄洜绲勭殑绲勬垚鍙婂叾鐗瑰緛
鍘熸牳鐢熺墿鍩哄洜绲勫強鍏剁壒寰�
鈼� DNA 鐨勮綁(zhu菐n)搴�
杞�(zhu菐n)搴у瓙鐨勫垎椤�(l猫i)鍜岀祼(ji茅)妲�(g貌u)鐗瑰緛
杞�(zhu菐n)搴т綔鐢ㄧ殑姗�(j墨)鍒�
杞�(zhu菐n)搴т綔鐢ㄧ殑閬哄偝瀛�(xu茅)鏁堟噳(y墨ng)
鐪熸牳鐢熺墿涓殑杞�(zhu菐n)搴у瓙
杞�(zhu菐n)搴у瓙 Tn10 鐨勮(di脿o)鎺ф(j墨)鍒�
鑰冭│瑕佹眰
鈼� 鐞嗚В DNA 鐨勫京(f霉)鍒跺拰 DNA 鐨勬悕鍌蜂慨寰�(f霉)鍩烘湰閬�(gu貌)绋嬪拰鍒嗗瓙姗�(j墨)鍒�
鈼� 鎺屾彙鍙冭垏 DNA 寰�(f霉)鍒剁殑閰惰垏铔嬬櫧璩�(zh矛)鍥犲瓙鐨勬€ц唱(zh矛)鍜岀ó椤�(l猫i)
鈼� 鎺屾彙 DNA 寰�(f霉)鍒剁殑鐗归粸(di菐n)鍙婂叾瑾�(di脿o)鎺ф(j墨)鍒�
鈼� 鎺屾彙鐪熸牳鐢熺墿鑸囧師鏍哥敓鐗� DNA 寰�(f霉)鍒剁殑鐣板悓榛�(di菐n)
鈼� 鍏ㄩ潰浜嗚В RNA 杞�(zhu菐n)閷勮垏寰�(f霉)鍒剁殑姗�(j墨)鍒�
鈼� 鎺屾彙杞�(zhu菐n)閷勭殑涓€鑸(gu墨)寰�
鈼� 鎺屾彙 RNA 鑱氬悎閰剁殑浣滅敤姗�(j墨)鐞�
鈼� 鐞嗚В鍘熸牳鐢熺墿鐨勮綁(zhu菐n)閷勯亷(gu貌)绋�
鈼� 鎺屾彙鍟熷嫊(d貌ng)瀛愬拰澧炲挤(qi谩ng)瀛愮殑浣滅敤姗�(j墨)鐞�
鈼� 浜嗚В鐪熸牳鐢熺墿鐨勮綁(zhu菐n)閷勯亷(gu貌)绋�
鈼� 鐞嗚В RNA 杞�(zhu菐n)閷勫悗鍔犲伐閬�(gu貌)绋嬪強鍏舵剰缇�
鈼� 鎺屾彙閫嗚綁(zhu菐n)閷勭殑閬�(gu貌)绋�
鈼� 浜嗚В鏌撹壊楂斿拰鍩哄洜绲勭殑姒傚康
鈼� 鎺屾彙 RNA 鍌抽仦鍔犲伐閬哄偝淇℃伅
鈼� 鎺屾彙 DNA 鐨勪慨寰�(f霉)銆佽綁(zhu菐n)搴у強澶氭厠(t脿i)鎬х殑鍩烘湰鍘熺悊鍙婂叾鎳�(y墨ng)鐢�
2. 铔嬬櫧璩�(zh矛)鐨勫悎鎴�
鑰冭│鍏�(n猫i)瀹�
鈼� 铔嬬櫧璩�(zh矛)鍚堟垚鐨勪竴鑸壒寰�
鈼� 妯℃澘銆佹サ鎬�銆侀伜鍌冲瘑纰肩殑鐗归粸(di菐n)
鈼� 鍙冭垏铔嬬櫧璩�(zh矛)鍚堟垚鐨勪富瑕佸垎瀛愮殑绋(l猫i)鍜屽姛鑳�
鈼� 铔嬬櫧璩�(zh矛)鍚堟垚鐨勭敓鐗╁(xu茅)姗�(j墨)鍒跺強鍏惰(di脿o)鎺ф(j墨)鍒�
鈼� 鑲介張鐨勫悗鍔犲伐閬�(gu貌)绋�
鈼� 鐪熸牳鐢熺墿鑸囧師鏍哥敓鐗╄泲鐧借唱(zh矛)鍚堟垚鐨勭壒榛�(di菐n)鍙婄暟鍚�
鈼� 铔嬬櫧璩�(zh矛)鍚堟垚鐨勬姂鍒跺洜瀛愬強鍏舵噳(y墨ng)鐢�
鑰冭│瑕佹眰
鈼� 鍏ㄩ潰浜嗚В铔嬬櫧璩�(zh矛)鍚堟垚鐨勯亷(gu貌)绋�
鈼� 鐔熺反鎺屾彙铔嬬櫧璩�(zh矛)鍚堟垚涓ā鏉垮拰閬哄偝瀵嗙⒓鐨勭壒榛�(di菐n)
鈼� 鎺屾彙铔嬬櫧璩�(zh矛)鍚堟垚鐨勪竴鑸壒寰�
鈼� 鎺屾彙鍙冭垏铔嬬櫧璩�(zh矛)鍚堟垚鐨勪富瑕佸垎瀛愮殑绋(l猫i)鍜屽姛鑳�
鈼� 鎺屾彙铔嬬櫧璩�(zh矛)鍚堟垚鐨勯亷(gu貌)绋嬪拰鑲介張鐨勫悗鍔犲伐閬�(gu貌)绋�
鈼� 鐞嗚В鐪熸牳鐢熺墿鑸囧師鏍哥敓鐗╄泲鐧借唱(zh矛)鍚堟垚鐨勭壒榛�(di菐n)鍙婄暟鍚�
鈼� 鐞嗚В铔嬬櫧璩�(zh矛)鍚堟垚鐨勬姂鍒跺洜瀛愬強鍏舵噳(y墨ng)鐢�
3. 鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺�
鑰冭│鍏�(n猫i)瀹�
鈼� 鍘熸牳鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺�
鍘熸牳鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺х附璜�
杞�(zhu菐n)閷勮(di脿o)绡€(ji茅)鐨勯(l猫i)鍨嬪強鍏剁壒寰�
鍟熷嫊(d貌ng)瀛愯垏杞�(zhu菐n)閷勮捣濮嬶紙瑕佹眰鐔熺反鎺屾彙锛岄潏娲婚亱(y霉n)鐢級
RNA 鑱氬悎閰惰垏鍟熷嫊(d貌ng)瀛愮殑鐩镐簰浣滅敤
鐠�(hu谩n)鑵鸿嫹閰稿彈楂旇泲鐧藉皪(du矛)杞�(zhu菐n)閷勭殑瑾�(di脿o)鎺�
涔崇硸鎿嶇副瀛愯垏璨�(f霉)鎺ц獦灏�(d菐o)绯荤当(t菕ng)
閰剁殑瑾樺皫(d菐o)鈥斺€攍ac 楂旂郴鍙楄(di脿o)鎺х殑璀夋摎(j霉)
鎿嶇副瀛愭ā鍨嬶紙瑕佹眰鐔熺反鎺屾彙锛岄潏娲婚亱(y霉n)鐢級
lac 鎿嶇副瀛� DNA 鐨勮(di脿o)鎺у崁(q奴)鍩�
lac 鎿嶇副瀛愪腑鐨勫叾浠栧晱(w猫n)椤�
鑹叉皑閰告搷绺卞瓙鑸囪矤(f霉)鎺ч樆閬忕郴绲�(t菕ng)
trp 鎿嶇副瀛愮殑闃婚亸绯荤当(t菕ng)
寮卞寲瀛愯垏鍓嶅皫(d菐o)鑲�
trp 鎿嶇副瀛愬急鍖栨(j墨)鍒剁殑瀵�(sh铆)椹�(y脿n)渚濇摎(j霉)
闃婚亸浣滅敤鑸囧急鍖栦綔鐢ㄧ殑鍗�(xi茅)瑾�(di脿o)
鍏朵粬鎿嶇副瀛�
鍗婁钩绯栨搷绺卞瓙
闃挎媺浼硸鎿嶇副瀛�
绲勬皑閰告搷绺卞瓙
recA 鎿嶇副瀛�
澶氬暉鍕�(d貌ng)瀛愯(di脿o)鎺х殑鎿嶇副瀛�
位 鍣弻楂斿熀鍥犺〃閬�(d谩)瑾�(di脿o)鎺�
位 鍣弻楂�
位 鍣弻楂斿熀鍥犵祫
婧跺師鍖栧惊鐠�(hu谩n)鍜屾憾鑿岄€斿緫鐨勫缓绔�
0 鍗€(q奴)
位 鍣弻楂旂殑瑾�(di脿o)鎺у崁(q奴)鍙娢� 闃婚亸鐗╃殑鐧�(f膩)鐝�(xi脿n)
C I 铔嬬櫧鍜� Cro 铔嬬櫧
杞�(zhu菐n)閷勫悗瑾�(di脿o)鎺�
绋€鏈夊瘑纰煎瓙灏�(du矛)缈昏鐨勫奖闊�
閲嶇枈鍩哄洜?q奴)唯顓恎鐨勫奖闊�
Poly(A) 灏�(du矛)缈昏鐨勫奖闊�
缈昏鐨勯樆閬�
mRNA 鑷韩绲�(ji茅)妲�(g貌u)鍘熶欢灏�(du矛)缈昏鐨勮(di脿o)鎺�
mRNA 绌�(w臎n)瀹氭€у皪(du矛)杞�(zhu菐n)閷勬按骞崇殑褰遍熆
瑾�(di脿o)绡€(ji茅)铔嬬櫧鐨勮(di脿o)鎺т綔鐢�
鍙嶇京 RNA 鐨勮(di脿o)绡€(ji茅)浣滅敤
RNA-RNA 鐩镐簰浣滅敤灏�(du矛)缈昏鐨勫奖闊�
榄旀枒鏍歌嫹閰告按骞冲皪(du矛)缈昏鐨勫奖闊�
杞�(zhu菐n)閷勬按骞崇殑鍏朵粬瑾�(di脿o)鎺ф柟寮�
S 鍥犲瓙鐨勮(di脿o)绡€(ji茅)浣滅敤
绲勮泲鐧介(l猫i)浼艰泲鐧界殑瑾�(di脿o)绡€(ji茅)浣滅敤
杞�(zhu菐n)閷勮(di脿o)鎺у洜瀛愮殑浣滅敤
鎶楃祩姝㈠洜瀛愮殑瑾�(di脿o)绡€(ji茅)浣滅敤
鈼� 鐪熸牳鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺х浉闂�(gu膩n)姒傚康鍜屼竴鑸(gu墨)寰�
鐪熸牳绱�(x矛)鑳炵殑鍩哄洜绲�(ji茅)妲�(g貌u)
鍩哄洜瀹舵棌锛坓ene family锛�
鐪熸牳鍩哄洜鐨勬柗瑁傜祼(ji茅)妲�(g貌u)
鐪熸牳鍩哄洜琛ㄩ仈(d谩)鐨勬柟寮忓強鐗归粸(di菐n)
鐪熸牳鍩哄洜琛ㄩ仈(d谩)鐨勪竴鑸(gu墨)寰�
鐪熸牳鍩哄洜琛ㄩ仈(d谩)鐨勮綁(zhu菐n)閷勬按骞宠(di脿o)鎺�
闋嗗紡浣滅敤鍏冧欢鑸囧熀鍥犺(di脿o)鎺�
Britten-Davidson 妯″瀷
鏌撹壊璩�(zh矛)绲�(ji茅)妲�(g貌u)灏�(du矛)杞�(zhu菐n)閷勭殑褰遍熆
鍟熷嫊(d貌ng)瀛愬強鍏跺皪(du矛)杞�(zhu菐n)閷勭殑褰遍熆
澧炲挤(qi谩ng)瀛愬強鍏跺皪(du矛)杞�(zhu菐n)閷勭殑褰遍熆
鍙嶅紡浣滅敤鍥犲瓙灏�(du矛)杞�(zhu菐n)閷勭殑瑾�(di脿o)鎺�
CAAT 鍗€(q奴)绲�(ji茅)鍚堣泲鐧� CTF/NF1
TATA 鍜� GC 鍗€(q奴)绲�(ji茅)鍚堣泲鐧�
RNA 鑱氬悎閰� III 鍙婂叾涓嬫父鍟熷嫊(d貌ng)鍗€(q奴)绲�(ji茅)鍚堣泲鐧�
鍏朵粬杞�(zhu菐n)閷勫洜瀛愬強鍒嗗瓙姗�(j墨)鍒�
鏌撹壊璩�(zh矛)绲�(ji茅)妲�(g貌u)鑸囧熀鍥犺綁(zhu菐n)閷勮(di脿o)鎺�
琛ㄨ閬哄偝瑾�(di脿o)鎺х殑鍩烘湰姒傚康
DNA 姘村钩涓婄殑瑾�(di脿o)鎺�
鏌撹壊璩�(zh矛)绲�(ji茅)妲�(g貌u)鑸� DNA 鐨勫彲鎺ヨ繎鎬у皪(du矛)杞�(zhu菐n)閷勭殑瑾�(di脿o)鎺�
DNA 鐢插熀鍖栬垏鍩哄洜杞�(zhu菐n)閷勮(di脿o)鎺�
鍩哄洜鎿�(ku貌)澧�
鍩哄洜閲嶆帓鑸囦氦鎻�
RNA 骞叉摼鐨勪竴鑸亷(gu貌)绋�
miRNA 鐨勭敓鐗╁(xu茅)閬�(gu貌)绋�
鏌撹壊璩�(zh矛)姘村钩鐨勮(di脿o)鎺�
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鈼� 鐔熺反鎺屾彙鐪熸牳鐢熺墿 DNA 姘村钩鐨勮(di脿o)鎺�
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鈼� 鐞嗚В DNA 鍏嬮殕鐨勫熀鏈師鐞�
鈼� 鎺屾彙鍚勭ó姘村钩涓婄殑鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺�
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鈼� 浜哄厤鐤己鎼嶇梾姣掆€斺€擧IV
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鈼� SV40 鐥呮瘨
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