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鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ(xu茅)鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗�锛岄儴鍒嗗(xu茅)鏍℃洿鏂拌嚦2012骞达紝2013骞�锛涘潎鎻愪緵鏀惰不(f猫i)涓嬭級銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛�(di菐n)鎿娾€�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛�(di菐n)鎿娾€�鑰冨崥瑭﹀嵎搴�鈥� 涓嬭級
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涓湅绉戝(xu茅)闄㈠ぇ瀛�(xu茅)纰╁＋鐮旂┒鐢熷叆瀛�(xu茅)鑰冭│
銆婇伜鍌冲(xu茅)銆嬭€冭│澶х侗
鏈€婇伜鍌冲(xu茅)銆嬭€冭│澶х侗閬╃敤浜庝腑鍦嬬瀛�(xu茅)闄㈠ぇ瀛�(xu茅)鐢熷懡绉戝(xu茅)鐩搁棞(gu膩n)灏堟キ(y猫)鐨勭ⅸ澹爺绌剁敓鍏ュ(xu茅)
鑰冭│銆傞伜鍌冲(xu茅)鐨勪富瑕佸収(n猫i)瀹瑰寘鎷稉(j墨ng)鍏搁伜鍌冲(xu茅)銆佺窗(x矛)鑳為伜鍌冲(xu茅)銆佸垎瀛愰伜鍌冲(xu茅)鍜岀櫦(f膩)鑲查伜鍌冲(xu茅)绛�銆�
瑕佹眰鑰冪敓鎺屾彙鍩烘湰姒傚康銆佸師鐞�锛屽緸鍊嬮珨銆佺窗(x矛)鑳炪€佸拰鍒嗗瓙姘村钩灏嶉伜鍌冲(xu茅)鏈夎純瀹屾暣鍜岀郴绲�(t菕ng)鐨�
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閬�(y霉n)鐢ㄦ墍瀛�(xu茅)鐭ヨ瓨鍒嗘瀽鍟忛鍜岃В姹哄晱椤�銆�
涓€銆佽€冭│绉戠洰鍩烘湰瑕佹眰鍙婇仼鐢ㄨ寖鍦嶆杩�
鐔熺反鎺屾彙閬哄偝瀛�(xu茅)鐨勫熀鏈師鐞嗚垏鐭ヨ瓨锛屼簡瑙ｉ伜鍌冲(xu茅)鐮旂┒鐨勬柊閫�(j矛n)灞曡垏鏂版蹇碉紝浜嗚В閬哄偝
瀛�(xu茅)鐮旂┒鐩搁棞(gu膩n)鐨勬柊鎶€琛�(sh霉)銆�
浜�銆佽€冭│褰㈠紡鍜岃│鍗风祼(ji茅)妲�(g貌u)锛堥鍨嬶級
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瑭﹀嵎绲�(ji茅)妲�(g貌u)锛氬悕瑭炶В閲嬶紝绨＄瓟椤岋紝閬哄偝瀛�(xu茅)瑷堢畻椤�
涓�銆佽€冭│鍏�(n猫i)瀹硅垏瑕佹眰
锛堜竴锛夋煋鑹查珨閬哄偝瀛�(xu茅)鑸囩窗(x矛)鑳為伜鍌冲(xu茅)
1. 鐞嗚В绱�(x矛)鑳炲垎瑁傜殑閬庣▼鑸囨剰缇�锛�
2. 鎺屾彙鏈夌挡鍒嗚鑸囨笡鏁�(sh霉)鍒嗚鐨勭暟鍚岋紝浜嗚В鏌撹壊楂斿湪鏈夌挡鍒嗚鍜屾笡鏁�(sh霉)鍒嗚涓殑琛�
鐐�锛�
3. 浜嗚В鏋滆爡鍞炬恫鑵烘煋鑹查珨鐨勭壒寰佸拰褰㈡垚鍘熷洜锛�
4. 鎺屾彙鏌撹壊楂斿(xu茅)瑾殑涓昏鍏�(n猫i)瀹�锛�
5. 鎺屾彙鐪熸牳鐢熺墿鏌撹壊楂旂殑绲勮鑸囩祼(ji茅)妲�(g貌u)妯″瀷銆�
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锛堜簩锛� 缍�(j墨ng)鍏搁伜鍌冲(xu茅)
1锛庣啛绶存帉鎻″瓱寰风埦鐨勯伜鍌冲垎闆㈠畾寰嬪拰閬哄偝鑷敱绲勫悎瀹氬緥鐨勫師鐞�锛�
2锛庝簡瑙ｆ€ф煋鑹查珨姹哄畾鎬у垾鐨勪富瑕侀鍨�锛岀悊瑙ｄ即鎬ч伜鍌宠(gu墨)寰嬶紱
3锛庣啛绶撮亱(y霉n)鐢ㄥ熀鍥犵殑閫ｉ帠鑸囦氦鎻涘畾寰嬮€�(j矛n)琛岄噸绲勯牷鐜囩殑瑷堢畻锛屾帉鎻′笁榛�(di菐n)娓氦娉曠殑鍘�
鐞嗚垏鎳�(y墨ng)鐢紱
4锛庢帉鎻¤瓬绯荤殑閬哄偝鍒嗘瀽鏂规硶锛�
5锛庣悊瑙ｉ伜鍌充簰瑁�(b菙)妾㈡脯鐨勫師鐞�锛岀啛绶存帉鎻￠伜鍌充簰瑁�(b菙)妾㈡脯鐨勫師鐞嗚垏鎳�(y墨ng)鐢�锛�
6锛庣悊瑙ｅ姂閲忚(b菙)鍎熸晥鎳�(y墨ng)鐨勬蹇�锛�
7锛庣啛绶存帉鎻″熀鍥犲瀷(genotype)銆佽〃鐝�(xi脿n)鍨�(phenotype)銆佸椤巼(penetrance)銆佽〃鐝�(xi脿n)搴�
(expressivity)绛夋蹇碉紝鎺屾彙琛ㄥ瀷姣旂巼鐨勮▓绠楁柟娉�锛�
8锛庢帉鎻＄瓑浣嶅熀鍥犮€佸京(f霉)绛変綅鍩哄洜銆侀潪绛変綅鍩哄洜绛夋蹇�锛�
9锛庝簡瑙ｅ熀鍥犵獊璁婁簰浣滅殑涓昏椤炲瀷鑸囧師鐞嗐€�
锛堜笁锛夊熀鍥犺垏鍩哄洜绲勭殑绲�(ji茅)妲�(g貌u)鑸囧姛鑳�
1. 鐔熺反鎺屾彙 DNA 闆欒灪鏃嬫ā鍨�銆備簡瑙� DNA 鐨勫叾瀹冩(g貌u)鍨嬶紱
2. 浜嗚В鍩哄洜姒傚康鐨勭櫦(f膩)灞�锛屾帉鎻″熀鍥犵殑椤炲瀷锛岀悊瑙ｅ熀鍥犺垏 DNA 鐨勯棞(gu膩n)绯伙紱
3. 鎺屾彙鍩哄洜绲勭祼(ji茅)妲�(g貌u)鐗归粸(di菐n)鍜屽姛鑳界殑灏嶆噳(y墨ng)闂�(gu膩n)绯�锛�
4. 鐞嗚В绛変綅鍩哄洜銆佺瓑浣嶇獊璁婄殑鎬ц唱(zh矛)鑸囩壒榛�(di菐n)锛�
5. 浜嗚В鐪熸牳鐢熺墿銆佸師鏍哥敓鐗╁熀鍥犵祫搴忓垪鐨勯鍨嬭垏鐗归粸(di菐n)锛�
6. 鐞嗚В鍩哄洜瀹舵棌鐨勬蹇碉紱
7. 浜嗚В鍩哄洜鐨勪笩澶�銆佹摯(ku貌)澧�銆侀噸鎺掔殑鐗归粸(di菐n)鑸囨剰缇�锛�
8. 鎺屾彙閲嶇祫娓閫�(j矛n)琛屽熀鍥犲畾浣嶇殑鍘熺悊锛岀悊瑙ｅ湒浣嶅厠闅嗙殑鍘熺悊锛屼簡瑙ｉ伜鍌虫嫰鏁�
(rescue)鎴栧垎瀛愰伜鍌充簰瑁�(b菙)瀵﹂纰哄畾鍩哄洜鍔熻兘鐨勫師鐞�銆�
锛堝洓锛夐伜鍌抽噸绲勮垏閬哄偝鍒嗘瀽
1. 鎺屾彙鍚屾簮閲嶇祫銆佷綅榛�(di菐n)鐗圭暟閲嶇祫鐨勫師鐞嗚垏鐗归粸(di菐n)锛�
2. 鐔熺反鎺屾彙閬哄偝閲嶇祫浣滃湒鐨勫師鐞嗚垏鎳�(y墨ng)鐢�锛�
3. 鎺屾彙缂哄け浣滃湒鐨勫師鐞嗗拰鏂规硶锛�
4. 鎺屾彙鍩哄洜杞�(zhu菐n)璁�(conversion)鐨勬蹇靛拰鍒嗗瓙姗�(j墨)鍒�锛�
5. 鐞嗚В閬哄偝妯�(bi膩o)瑷樼殑鍘熺悊銆佺壒榛�(di菐n)鑸囨噳(y墨ng)鐢�锛�
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6. 浜嗚В绱�(x矛)鑿屽拰鍣弻楂斿熀鍥犻噸绲勮垏閲嶇祫浣滃湒鐨勫師鐞�锛�
7. 鎺屾彙杞�(zhu菐n)搴у瓙鑸囬€嗚綁(zhu菐n)搴у瓙鐨勭祼(ji茅)妲�(g貌u)鐗归粸(di菐n)锛岃綁(zhu菐n)搴фā鍨�锛岀悊瑙ｅ叾鐨勯伜鍌虫晥鎳�(y墨ng)锛屼簡瑙ｈ綁(zhu菐n)
搴у瓙鑸囬€嗚綁(zhu菐n)搴у瓙鐨勪富瑕侀鍨嬨€�
锛堜簲锛夊熀鍥犺〃閬�(d谩)鍜岃(di脿o)鎺�
1. 鐔熺反鎺屾彙涓績娉曞墖锛�
2. 鐞嗚В闋嗗弽瀛愭蹇靛拰闋嗗弽瀛愭ā鍨�锛�
3. 浜嗚В涔崇硸鎿嶇副瀛愮殑瑾�(di脿o)鎺фā鍨�锛�
4. 鐞嗚В杞�(zhu菐n)閷勮捣濮嬨€佺祩姝㈢殑绲�(ji茅)妲�(g貌u)鐗归粸(di菐n)鍜屼綔鐢ㄦ(j墨)鐞�锛�
5. 鎺屾彙杞�(zhu菐n)閷勯爢寮�銆佸弽寮忎綔鐢ㄥ厓浠剁殑椤炲瀷鍜屽姛鑳�锛�
6. 浜嗚В DNA 閲嶆帓瑾�(di脿o)鎺у熀鍥犺〃閬�(d谩)鍘熺悊鍜屾(j墨)鍒�锛�
7. 鎺屾彙 RNA 鍓嶉珨鍔犲伐鐨勫師鐞�銆佷富瑕侀鍨嬭垏鎰忕京锛屼簡瑙� RNA 鍓嶉珨鍙畩鍓垏鐨勪富
瑕侀鍨嬭垏鎰忕京銆備簡瑙� RNA 绶ㄨ集鐨勬蹇靛拰鍘熺悊锛�
8. 鎺屾彙 mRNA 鐨勭祼(ji茅)妲�(g貌u)鐗归粸(di菐n)鍜屼綔鐢�锛屼簡瑙ｇ炕璀捣濮嬪洜瀛愯垏铔嬬櫧璩�(zh矛)鍚堟垚璧峰鍙嶆噳(y墨ng)
鐨勮(di脿o)鎺�锛�
9. 浜嗚В DNA 鐢插熀鍖�銆佸幓鐢插熀鍖�銆佺祫铔嬬櫧淇＞绛夎〃瑙€閬哄偝淇＞瑾�(di脿o)鎺х殑涓昏椤炲瀷銆�
浣滅敤姗�(j墨)鍒惰垏鎰忕京锛屼簡瑙ｅ弽缇� RNA銆佸皬 RNA 鍒嗗瓙鐨勪富瑕侀鍨嬭垏瑾�(di脿o)鎺у熀鍥犺〃閬�(d谩)
鐨勬(j墨)鍒�銆�
锛堝叚锛夐伜鍌崇墿璩�(zh矛)鐨勬敼璁�
1. 鎺屾彙绐佽畩鐨勬蹇�銆佺獊璁婇鍨嬪強鍏跺垎瀛愬熀绀�(ch菙)锛�
2. 鎺� 鎻� 鏌� 鑹� 楂� 缂� 澶� (deletion) 銆� 閲� 寰�(f霉) (duplication) 銆� 鍊� 浣� (inversion) 銆� 鏄� 浣�
(translocation)鐨勭壒榛�(di菐n)锛岀櫦(f膩)鐢熺殑姗�(j墨)鍒跺拰閬哄偝鏁堟噳(y墨ng)锛�
3. 鎺屾彙鏌撹壊楂旀暩(sh霉)鐩畩鐣扮殑鍩烘湰椤炲瀷锛屽舰鎴愬師鍥犺垏閬哄偝鏁堟噳(y墨ng)锛�
4. 浜嗚В鏌撹壊楂旇畩鐣板湪閫�(j矛n)鍖栦腑鐨勬剰缇�锛�
5. 浜嗚В鑷櫦(f膩)绐佽畩銆佽獦鐧�(f膩)绐佽畩銆佷汉宸ュ悓婧愰噸绲勭瓑鍩哄洜绲勪慨椋炬柟娉曠殑鍒嗗瓙姗�(j墨)鍒�锛�
6. 鐔熸倝鍏夊京(f霉)娲�銆佸垏闄や慨寰�(f霉)銆侀噸绲勪慨寰�(f霉)銆丼OS 淇京(f霉)绛� DNA 淇京(f霉)鐨勫垎瀛愭ā鍨�銆�
锛堜竷锛夋暩(sh霉)閲忔€х媭鐨勯伜鍌冲垎鏋�
1. 鎺屾彙鏁�(sh霉)閲忔€х媭鐨勬蹇靛拰鐗瑰緛锛�
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2. 浜嗚В澶氬熀鍥�?q奴)W瑾殑鍏�(n猫i)瀹瑰拰澶氬熀鍥犳晥鎳�(y墨ng)锛�
3. 鐞嗚В閬哄偝鐜囩殑姒傚康锛屾帉鎻￠伜鍌崇巼鐨勮▓绠楁柟娉曪紱
4. 浜嗚В杩戜氦鐨勬蹇�锛屾帉鎻¤繎浜ょ郴鏁�(sh霉)鐨勮▓绠楁柟娉曪紝鐞嗚В闆滀氦鍎�(y艒u)鍕㈢殑鍏�(n猫i)娑�銆�
锛堝叓锛夐伜鍌宠垏閫�(j矛n)鍖�
1. 鐞嗚В瀛熷痉鐖剧兢楂斿拰鍩哄洜搴殑姒傚康锛屾帉鎻＄瓑浣嶅熀鍥犻牷鐜囧拰鍩哄洜鍨嬮牷鐜囩殑瑷堢畻鏂�
娉�锛屼簡瑙ｇ獊璁婂拰閬告搰灏嶅熀鍥犻牷鐜囩殑褰遍熆锛�
2. 浜嗚ВHardy-Weinberg瀹氬緥鐨勫収(n猫i)瀹�銆佸钩琛＄兢楂旂殑鍩烘湰鐗瑰緛銆佸奖闊� Hardy-Weinberg
骞宠 鐨勫洜绱�锛�
3. 浜嗚В閬哄偝婕傝畩鐨勬蹇�锛�
4. 浜嗚В鐗╃ó褰㈡垚鐨勯亷绋嬪拰鏂瑰紡锛�
5. 浜嗚В鏂板熀鍥犵嵅寰楃殑鏂瑰紡銆佸熀鍥犵祫閫�(j矛n)鍖栫殑鏂瑰紡锛�
6. 浜嗚В鍒嗗瓙閫�(j矛n)鍖栫殑涓€у(xu茅)瑾殑鍏�(n猫i)瀹广€�
(涔�) 鍩哄洜宸ョ▼
1. 鎺屾彙 PCR 鎶€琛�(sh霉)鐨勫師鐞�锛�
2. 鐞嗚В闄愬埗鎬у収(n猫i)鍒囬叾銆丏NA 鍚堟垚閰跺拰閫ｆ帴閰�銆佸弽杞�(zhu菐n)閷勯叾绛夌殑浣滅敤鍘熺悊銆佺敤閫�锛�
3. 鎺屾彙杞�(zhu菐n)鍩哄洜鐨勬蹇碉紝浜嗚В甯歌鐨勮綁(zhu菐n)鍩哄洜鎶€琛�(sh霉)鐨勫師鐞�銆�
鍥涖€佷富瑕佸弮鑰冩浉鐩�
1锛庛€婇伜鍌冲(xu茅)銆嬶紙绗� 3 鐗堬級锛屽妷绁栨礊绛夌法钁�锛岄珮绛夋暀鑲插嚭鐗堢ぞ锛�2013 骞�
2 锛� AN INTRODUCTION TO GENETIC ANALYSIS 锛� 缍�(w菐ng) 涓� 鍏� 璨�(f猫i) 闁� 璁€
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.TOC锛�
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