|
鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�(gu贸)400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ(xu茅)鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗�锛岄儴鍒嗗(xu茅)鏍℃洿鏂拌嚦2012骞�锛�2013骞�锛涘潎鎻愪緵鏀惰不(f猫i)涓嬭級銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛�(di菐n)鎿娾€�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛�(di菐n)鎿娾€�鑰冨崥瑭﹀嵎搴�(k霉)鈥� 涓嬭級
纰╁＋鐮旂┒鐢熷叆瀛�(xu茅)鑰冭│澶х侗
鑰冭│绉戠洰鍚嶇ū锛氬垎瀛愮敓鐗╁(xu茅)
涓€銆佹彺寮曟暀鏉�
銆婄従(xi脿n)浠ｅ垎瀛愮敓鐗╁(xu茅)銆� 绗洓鐗� 鏈辩帀璩� 楂樼瓑鏁欒偛鍑虹増绀�
浜�銆佽€冭│瑕佹眰锛�
瑕佹眰鑰冪敓鍏ㄩ潰绯荤当(t菕ng)鍦版帉鎻″垎瀛愮敓鐗╁(xu茅)鐨勬蹇�銆佺爺绌�?j墨)?n猫i)瀹硅垏鐗归粸(di菐n)锛涙帉鎻＄敓鍛芥椿鍕�(d貌ng)涓噸瑕佺殑
鐢熺墿澶у垎瀛愮殑绲�(ji茅)妲�(g貌u)鑸囧姛鑳�锛涙帉鎻″熀鍥犺垏鍩哄洜绲勭殑姒傚康锛屽師鏍哥敓鐗╄垏鐪熸牳鐢熺墿鍩哄洜绲勭殑绲�(ji茅)妲�(g貌u)鍙�
鐗归粸(di菐n)锛涚悊瑙� DNA 寰�(f霉)鍒剁殑鍗婁繚鐣欐(j墨)鍒跺拰鍗婁笉閫ｇ簩(x霉)寰�(f霉)鍒讹紱鎺屾彙 DNA 寰�(f霉)鍒剁殑涓€鑸壒榛�(di菐n)锛涚悊瑙ｈ綁(zhu菐n)
閷勭殑鍩烘湰閬�(gu貌)绋嬨€佹(j墨)鍒�锛涙帉鎻＄湡鏍� RNA 鐨勮綁(zhu菐n)閷勫悗鍔犲伐锛涙帉鎻¤泲鐧借唱(zh矛)鍚堟垚鐨勭敓鐗╁(xu茅)姗�(j墨)鍒�锛涙帉鎻�
鍘熸牳鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺фā寮忥紱鎺屾彙鐪熸牳鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺х殑涓€鑸(gu墨)寰�锛涗簡瑙ｇ敓鍛界瀛�(xu茅)鐧�(f膩)灞曠殑鏂瑰悜鑸囧墠
娌匡紝浜嗚В鍒嗗瓙鐢熺墿瀛�(xu茅)鍦ㄦ湁闂�(gu膩n)闋�(l菒ng)鍩熺殑鎳�(y墨ng)鐢ㄨ垏鍓嶆櫙銆�
涓�銆佽€冭│鍏�(n猫i)瀹癸細
绗竴绔� 绶掕珫
鍒嗗瓙鐢熺墿瀛�(xu茅)瀹氱京锛涘垎瀛愮敓鐗╁(xu茅)鐧�(f膩)灞曠啊(ji菐n)鍙诧細鍒嗗瓙鐢熺墿瀛�(xu茅)瀹氱京锛涗簡瑙ｅ垎瀛愮敓鐗╁(xu茅)鐧�(f膩)灞曠啊(ji菐n)鍙�锛�
鎺屾彙鐮旂┒鍒嗗瓙鐢熺墿瀛�(xu茅)鍏�(n猫i)瀹癸紱浜嗚В鍒嗗瓙鐢熺墿瀛�(xu茅)鐨勭櫦(f膩)灞曞墠鏅�銆�
绗簩绔� 鍩哄洜姒傚康鐨勬紨璁婅垏鐧�(f膩)灞�
鍩哄洜鐨勬蹇�锛涘熀鍥犵殑鍒嗗瓙绲�(ji茅)妲�(g貌u)锛氭牳鑻风殑鍒嗗瓙妲�(g貌u)璞�锛孌NA 闆欒灪鏃嬬祼(ji茅)妲�(g貌u)妯″瀷锛孌NA 鐨勮畩
鎬ц垏寰�(f霉)鎬�锛涙牳閰稿垎瀛愮殑绌洪枔绲�(ji茅)妲�(g貌u)锛欴NA 鐨勪竴绱�(j铆)锛屼簩绱�(j铆)锛屼笁绱�(j铆)绲�(ji茅)妲�(g貌u)锛涘熀鍥犳蹇电殑澶氭ǎ鎬э細閲�
鐤婂熀鍥�锛岄枔闅斿熀鍥�锛岃烦韬嶅熀鍥狅紝鍋囧熀鍥�銆�
浜嗚В鍩哄洜鐨勬蹇�锛涚悊瑙� DNA 闆欒灪鏃嬬祼(ji茅)妲�(g貌u)妯″瀷, 鎺屾彙鍩哄洜鐨勫垎瀛愮祼(ji茅)妲�(g貌u)锛涚悊瑙� DNA 鐨勪竴
绱�(j铆)锛屼簩绱�(j铆)锛屼笁绱�(j铆)绲�(ji茅)妲�(g貌u)锛涙帉鎻￠噸鐤婂熀鍥狅紝闁撻殧鍩哄洜锛岃烦韬嶅熀鍥�锛屽亣鍩哄洜绛夋蹇�銆�
缍�(j墨ng)鍏哥殑鍩哄洜姒傚康锛屾牳閰稿垎瀛愮殑绌洪枔绲�(ji茅)妲�(g貌u)
绗笁绔� DNA 鐨勫京(f霉)鍒�
DNA 鐨勫京(f霉)鍒讹細DNA 鐨勫崐淇濈暀寰�(f霉)鍒舵(j墨)鐞�銆佸京(f霉)鍒剁殑璧烽粸(di菐n)銆佹柟鍚戝拰閫熷害锛涘師鏍哥敓鐗╁拰鐪熸牳
鐢熺墿 DNA 鐨勫京(f霉)鍒剁壒榛�(di菐n)锛氬師鏍哥敓鐗� DNA 鐨勫京(f霉)鍒剁壒榛�(di菐n)銆佺湡鏍哥敓鐗� DNA 鐨勫京(f霉)鍒剁壒榛�(di菐n)銆丏NA 寰�(f霉)
鍒剁殑瑾�(di脿o)鎺�銆�
鐞嗚В DNA 寰�(f霉)鍒剁殑鍗婁繚鐣欐(j墨)鍒跺拰鍗婁笉閫ｇ簩(x霉)寰�(f霉)鍒�锛涙帉鎻＄窗(x矛)鑿� DNA 寰�(f霉)鍒堕亷(gu貌)绋嬪強鏈夐噸瑕佷綔鐢�
鐨勯叾鍜岃泲鐧借唱(zh矛)锛涙帉鎻� DNA 寰�(f霉)鍒剁殑涓€鑸壒榛�(di菐n)锛涗簡瑙ｇ湡鏍哥敓鐗� DNA 寰�(f霉)鍒剁殑鐗归粸(di菐n)銆�
DNA 寰�(f霉)鍒剁殑鍗婁繚鐣欐(j墨)鍒跺拰鍗婁笉閫ｇ簩(x霉)寰�(f霉)鍒�锛涙帉鎻＄窗(x矛)鑿� DNA 寰�(f霉)鍒堕亷(gu貌)绋嬪強鏈夐噸瑕佷綔鐢ㄧ殑閰�
鍜岃泲鐧借唱(zh矛)锛涙帉鎻� DNA 寰�(f霉)鍒剁殑涓€鑸壒榛�(di菐n)銆�
绗洓绔� RNA 鐨勮綁(zhu菐n)閷�
RNA 鐨勮綁(zhu菐n)閷勶細杞�(zhu菐n)閷勭殑鍩烘湰閬�(gu貌)绋�銆佽綁(zhu菐n)閷勬(j墨)鍣ㄧ殑涓昏鎴愬垎锛涘暉鍕�(d貌ng)瀛愯垏杞�(zhu菐n)閷勮捣濮嬶細?ji菐n)顣�?d貌ng)瀛愬崁(q奴)
鐨勫熀鏈祼(ji茅)妲�(g貌u)銆�-10 鍗€(q奴)鍜�-35 鍗€(q奴)鐨勬渶浣抽枔璺�銆佸寮�(qi谩ng)瀛愬強鍏跺姛鑳姐€佺湡鏍哥敓鐗╁暉鍕�(d貌ng)瀛愬皪(du矛)杞�(zhu菐n)閷勭殑褰遍熆锛�
鍘熸牳鐢熺墿鑸囩湡鏍哥敓鐗� mRNA 鐨勭壒寰佹瘮杓冿細鍘熸牳鐢熺墿 mRNA 鐨勭壒寰併€佺湡鏍哥敓鐗� mRNA 鐨勭壒
寰�锛涚祩姝㈠拰鎶楃祩姝細涓嶄緷璩翠簬 蟻 鍥犲瓙鐨勭祩姝�銆佷緷璩翠簬 蟻 鍥犲瓙鐨勭祩姝�锛涘収(n猫i)鍚瓙鐨勫壀鎺ャ€佺法杓強
鍖栧(xu茅)淇＞锛歊NA 涓殑鍏�(n猫i)鍚瓙銆丷NA 鐨勫壀鎺�銆丷NA 鐨勭法杓拰鍖栧(xu茅)淇＞銆�
鎺屾彙杞�(zhu菐n)閷勭殑鍩烘湰閬�(gu貌)绋�銆佹(j墨)鍒�锛涙帉鎻＄湡鏍� RNA 鐨勮綁(zhu菐n)閷勫悗鍔犲伐銆�
杞�(zhu菐n)閷勬ā鏉跨殑涓嶅皪(du矛)绋辨€фサ鍏跺懡鍚�锛岃綁(zhu菐n)閷勭殑鍙嶆噳(y墨ng)楂旂郴锛� RNA 鐨勮綁(zhu菐n)閷勯亷(gu貌)绋嬪ぇ楂斿彲鍒嗙偤璧峰銆�
寤堕暦(zh菐ng)銆佺祩姝笁鍊�(g猫)闅庢銆傜湡鏍� RNA 鐨勮綁(zhu菐n)閷勫悗鍔犲伐銆�
绗簲绔� 铔嬬櫧璩�(zh矛)鐨勭炕璀�
閬哄偝瀵嗙⒓鈥斾笁鑱�(li谩n)瀛愶細涓夎伅(li谩n)瀛愬瘑纰煎強鍏剁牬璀�銆侀伜鍌冲瘑纰肩殑鎬ц唱(zh矛)锛� tRNA锛歵RNA 鐨� L 褰笁
绱�(j铆)绲�(ji茅)妲�(g貌u)銆乼RNA 鐨勫姛鑳�銆乼RNA 鐨勭ó椤�銆佹皑閰�-tRNA 鍚堟垚閰�锛涙牳绯栭珨锛氭牳绯栭珨鐨勭祼(ji茅)妲�(g貌u)銆佹牳绯�
楂旂殑鍔熻兘锛涜泲鐧借唱(zh矛)鍚堟垚鐨勭敓鐗╁(xu茅)姗�(j墨)鍒讹細姘ㄥ熀閰哥殑娲诲寲銆佺炕璀殑璧峰銆佽偨閺堢殑寤朵几銆佽偨閺堢殑绲�
姝€€佽泲鐧借唱(zh矛)鍓嶉珨鐨勫姞宸�銆佽泲鐧借唱(zh矛)鍚堟垚鎶戝埗鍔�銆�
浜嗚В閬哄偝瀵嗙⒓鐨勭壒鎬�銆乼RNA 鐨勭ó椤�锛涙帉鎻¤泲鐧借唱(zh矛)鍚堟垚鐨勭敓鐗╁(xu茅)姗�(j墨)鍒�銆佹帉鎻℃牳绯栭珨鐨勭祫
鎴�銆佺祼(ji茅)妲�(g貌u)鍜屽姛鑳�銆佽泲鐧借唱(zh矛)鍓嶉珨鐨勫姞宸ユ(j墨)鍒躲€�
绗叚绔� 鍩哄洜琛ㄩ仈(d谩)鐨勮(di脿o)鎺�
鍘熸牳鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺х殑鐞嗚珫鑸囨ā寮忥細鎿嶇副瀛愭ā鍨嬪強鍏跺奖闊垮洜瀛�锛屽垎瑙ｄ唬璎濈敘(ch菐n)鐗╅樆閬忓暉鍕�(d貌ng)瀛�
鐨勬鎺у埗绯荤当(t菕ng)锛岃“娓涘瓙鐨勭櫦(f膩)鐝�(xi脿n)鍙婂叾瑾�(di脿o)鎺�锛涗笉鍒╃敓闀�(zh菐ng)姊濅欢涓嬬殑鎳�(y墨ng)鎬ュ弽鎳�(y墨ng)锛氬毚(y谩n)绶婂弽鎳�(y墨ng)鐩搁棞(gu膩n)鍥犲瓙锛�
鍤�(y谩n)绶婂洜瀛愬弽鎳�(y墨ng)鐨勮(di脿o)鎺ф(j墨)鍒�锛涜綁(zhu菐n)閷勫悗姘村钩鐨勮(di脿o)鎺э細鐪熸牳鐢熺墿杞�(zhu菐n)閷勫悗锛涚炕璀按骞充笂鐨勮(di脿o)鎺�锛涚炕璀�
鍚庣殑鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺э紱鐪熸牳鐢熺墿鐨勫熀鍥犺〃閬�(d谩)瑾�(di脿o)鎺х殑鐗规畩椤炲瀷銆�
鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺х殑鍩烘湰姒傚康锛涙帉鎻″師鏍稿熀鍥犺(di脿o)鎺ф(j墨)鍒剁殑椤炲瀷鑸囩壒榛�(di菐n)锛涙帉鎻′钩绯栨搷绺卞瓙鑸囪矤(f霉)
鎺ц獦灏�(d菐o)绯荤当(t菕ng)锛涗簡瑙ｅ崐涔崇硸鎿嶇副瀛�銆侀樋鎷変集绯栨搷绺卞瓙锛涙帉鎻¤綁(zhu菐n)閷勫悗瑾�(di脿o)鎺�銆傛帉鎻＄湡鏍哥敓鐗╃殑鍩哄洜
绲�(ji茅)妲�(g貌u)鑸囪綁(zhu菐n)閷勬椿鎬�锛涙帉鎻＄湡鏍稿熀鍥犵殑鏂疯绲�(ji茅)妲�(g貌u)锛涙帉鎻＄湡鏍哥敓鐗� DNA 姘村钩涓婄殑鍩哄洜琛ㄩ仈(d谩)瑾�(di脿o)鎺�锛�
鎺屾彙 DNA 鐢插熀鍖栬垏鍩哄洜娲绘€х殑瑾�(di脿o)鎺�锛涙帉鎻� DNA 鐢插熀鍖栨姂鍒跺熀鍥犺綁(zhu菐n)閷勭殑姗�(j墨)鐞嗭紱鎺屾彙鐪熸牳鍩�
鍥犵殑杞�(zhu菐n)閷�锛涙帉鎻″叾浠栨按骞充笂鐨勫熀鍥犺(di脿o)鎺с€�
绗竷绔� 鍒嗗瓙鐢熺墿瀛�(xu茅)鏂规硶
鍩哄洜鍏�?銆傚く顙冭毄瀵恒€夊Ξd楂�锛屽伐鍏烽叾锛汸CR锛氬師鐞�锛孯T-PCR銆�
鍩哄洜鍏嬮殕鐨勯亷(gu貌)绋�锛涙帉鎻� PCR 鍘熺悊鍙婂父瑕�(ji脿n)鐨勭ó椤�锛涗簡瑙ｅ熀鍥犲厠闅嗙殑杓夐珨锛屽伐鍏烽叾銆�
鍏嶈铂(z茅)鑱叉槑锛氭湰鏂囩郴杞�(zhu菐n)杓夎嚜缍�(w菐ng)绲�(lu貌)锛屽鏈変镜鐘�锛岃珛(q菒ng)鑱�(li谩n)绯绘垜鍊戠珛鍗冲埅闄�锛屽彟锛氭湰鏂囧儏浠ｈ〃浣滆€呭€�(g猫)浜鸿榛�(di菐n)锛岃垏鏈恫(w菐ng)绔欑劇(w煤)闂�(gu膩n)銆傚叾鍘熷壍(chu脿ng)鎬т互鍙婃枃涓櫝杩版枃瀛楀拰鍏�(n猫i)瀹规湭缍�(j墨ng)鏈珯璀夊(sh铆)锛屽皪(du矛)鏈枃浠ュ強鍏朵腑鍏ㄩ儴鎴栬€呴儴鍒嗗収(n猫i)瀹�銆佹枃瀛楃殑鐪熷(sh铆)鎬�銆佸畬鏁存€с€佸強鏅�(sh铆)鎬ф湰绔欎笉浣滀换浣曚繚璀夋垨鎵胯锛岃珛(q菒ng)璁€鑰呭儏浣滃弮鑰�锛屽苟璜�(q菒ng)鑷鏍稿(sh铆)鐩搁棞(gu膩n)鍏�(n猫i)瀹�銆�
|
鏂囩珷鎼滅储
鎮ㄧ従(xi脿n)鍦ㄧ殑浣嶇疆锛� 
