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1銆佸病宕庣墖娈� 2銆佹皑閰� tRNA
3銆佸闋嗗弽瀛� mRNA 4銆丅LAST
5銆丏NA 寰╂€� 6銆佸弽瀵嗙⒓瀛�
7銆佸寮峰瓙 8銆丆pG 宄�
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2銆� DNA 鐢插熀鍖栧悗灏� 鍩哄洜鐨勮綁(zhu菐n)閷�銆�
3銆� 寰� Bacillus globigii 閲屽垎闆㈠嚭渚嗙殑绗� 2 绋檺鍒舵€у収(n猫i)鍒囬叾鎳夊懡鍚嶇偤 銆�
4銆� 鏌愰洐閺� DNA 鍒嗗瓙涓紝A 鐨勫惈閲忕偤 15%锛屽墖 C 鐨勫惈閲忔槸 銆�
5銆� 澶ц吀妗胯弻鍩哄洜绲勫ぇ灏� 4.6x106 bp锛岀敤闄愬埗鎬у収(n猫i)鍒囬叾 EcoRI (GAATTC) 灏嶅叾鍩哄洜绲勯€茶閰跺垏
娑堝寲锛岀悊璜栦笂鍙互鐢�(ch菐n)鐢� 鍊� DNA 鐗囨銆�
6銆� RNA 鐢熺墿鍚堟垚涓�锛孯NA 鑱氬悎閰剁殑娲绘€ч渶瑕� 妯℃澘锛屽師鏂欐槸 銆�
7銆� 鏄竴椤炲叿鏈夊偓鍖栨椿鎬х殑 RNA 鍒嗗瓙 銆�
8銆� 鑳藉舰鎴� DNA鈥擱NA 闆滀氦鍒嗗瓙鐨勭敓鐗╁悎鎴愰亷绋嬫湁 銆� 锛屽舰鎴愮殑鍒嗗瓙鍩虹
鏄� 銆�
9銆� 鍏嬮殕渚嗚嚜浜哄熀鍥犵祫绱� 500kb 闀风殑 DNA 鐗囨锛屾渶浣宠級楂旀槸 銆�
10銆丳CR 鍏ㄧū 锛岄€氶亷澶氭閲嶅京 銆� 鍜� 涓夊€嬮亷绋�锛屽湪楂斿鐛插緱澶ч噺鐩�
鐨� DNA 鐗囨銆傚吀鍨嬬殑 PCR 鍙嶆噳楂旂郴鍖呮嫭 銆� 銆� 銆� 銆佺珐娌栨恫鍜�
Mg2+
绛夌墿璩�(zh矛)銆�
11銆侀珨鍏�(n猫i) DNA 寰╁埗涓€鑸互 鐐哄紩鐗�锛屽京鍒舵柟鍚� 锛屽偓鍖栧紩鐗╁悎鎴愮殑閰剁ū鐐� 銆�
12銆佺檶鍩哄洜鍙垎鐐� 銆� 鍏╁ぇ椤�銆�
13銆佸ぇ鑵告】鑿屽湪閫茶閷厤淇京鏅�锛屼互 浣滅偤璀樺垾瀛愩€佹瘝閺堢殑妯欒锛岃€屽弮鑸囦慨寰╁悎鎴愮殑 DNA
鑱氬悎閰舵槸 銆�
14銆佷笁鍊� DNA 鐗囨 A銆丏1 鍜� D2 鍒嗗垾缃簬涓€鍊� FIN2 鍟熷嫊瀛愭帶鍒剁殑鏂伴湁绱犳姉鎬у熀鍥犵殑涓嶅悓浣�
缃笂锛屽涓嬪湒宸︽墍绀�锛涜唱(zh矛)绮掑垎鍒ヨ綁(zhu菐n)鏌撳皬榧犺厧绱拌優绯伙紝鎵€寰楃窗鑳炲厠闅嗗湪鍚湁 G418 鐨勫煿椁�(y菐ng)鍩�
鍏� 6 闋� 绗� 2 闋�
涓€茶鏂伴湁绱犳姉鎬х閬�锛屽叿鏈夋柊闇夌礌鎶楁€х殑鍏嬮殕鏁�(sh霉)濡備笅鍦栧彸鎵€绀�銆�
鏍规摎(j霉)瀵﹂绲�(ji茅)鏋滃彲浠ユ帹娓細 鐗囨鏄寮峰瓙锛� 鐗囨鏄矇榛樺瓙锛� 鐗囨鏄禃绶ｅ瓙銆�
涓�銆侀伕鎿囬锛�30 鍒嗭紝姣忛 1 鍒嗭級
1. 鍦ㄥ簭鍒� 5鈥�-CCGAATACATATGGAGT-3鈥欎腑鍚湁涓€鍊� 6bp 鐨� II 鍨嬮檺鍒舵€у収(n猫i)鍒囬叾鐨勮瓨鍒ュ簭鍒�锛�
瑭蹭綅榛炵殑搴忓垪鍙兘鏄粈涔�锛� ( )
A銆丄TACAT B銆丆ATATG C銆丟AATAC D銆丄CATAT
2. 涓嬪垪灏� DNA 闆欓張鎻忚堪姝ｇ⒑鐨勬槸 ( ) 銆�
A銆佷綔鐐烘ā鏉垮悎鎴� RNA 鐨� DNA 閺堣绋辩偤鍙嶇京閺堬紱
B銆丏NA 涓€姊濋張鐐烘湁缇╅張锛屽彟涓€姊濋張鍦ㄥ熀鍥犺〃閬斾腑娌掓湁瀛樺湪鐨勬剰缇�锛�
C銆佸崐淇濈暀寰╁埗鏄寚鍙皪鏈夌京閺堥€茶寰╁埗锛�
D銆丏NA 妯℃澘鐨勮綁(zhu菐n)閷勭敱 DNA 鑱氬悎閰朵締瀹屾垚銆�
3. 涓嬪垪鍝ó鍏嬮殕杓夐珨灏嶅婧� DNA 鐨勫杓夐噺鏈€澶�锛� ( )
A銆佽唱(zh矛)绮� B銆丆osmid C銆乊AC D銆佄� 鍣弻楂�
4. 澶ц吀妗胯弻涔崇硸鎿嶇副瀛愮殑杞�(zhu菐n)閷勬椿鎬у湪濡備笅鍝ó姊濅欢涓嬮仈鍒版渶楂� ( )
A銆侀珮涔崇硸锛屼綆钁¤悇绯� B銆侀珮涔崇硸锛岄珮钁¤悇绯�
C銆佷綆涔崇硸锛屼綆钁¤悇绯� D銆佷綆涔崇硸锛岄珮钁¤悇绯�
5. 鍚屼竴绋唱(zh矛)绮� DNA锛屼互瓒呰灪鏃嬶紙SC锛�銆侀枊鐠�(hu谩n)锛圤C锛夊拰绶氭€э紙L锛夊垎瀛愪笁绋笉鍚岀殑褰㈠紡瀛樺湪锛�
闆绘吵鏅�锛屽畠鍊戠殑閬风Щ閫熺巼鏄細( )
A銆丱C DNA>SC DNA>L DNA B銆丼C DNA>OC DNA>L DNA
C銆丩 DNA>OC DNA>SC DNA D銆丼C DNA>L DNA>OC DNA
6. 鐢ㄩ€ｆ帴閰跺皣 Sau3A I ( ^GATC) 鍒囧壊鐨� DNA 鑸囩稉(j墨ng) BamHI (G^GATCC) 鍒囧壊鐨� DNA 閫ｆ帴
鍚庯紝鑳藉琚� BamHI 鍐嶆鍒囧壊闁嬬殑姒傜巼鏈夊澶�锛� ( )
A銆�0 B銆�25% C銆�50% D銆�100%
7. 绔矑閰舵槸涓€绋� ( )
A銆佷緷璩翠簬 DNA 鐨� DNA 鑱氬悎閰� B銆佷緷璩翠簬 DNA 鐨� RNA 鑱氬悎閰�
C銆佷緷璩翠簬 RNA 鐨� DNA 鑱氬悎閰� D銆佷緷璩翠簬 RNA 鐨� RNA 鑱氬悎閰�
8. 涓嬪垪灏嶅熀鍥犳蹇垫弿杩版纰虹殑鏄� ( )
鍏� 6 闋� 绗� 3 闋�
A銆佸熀鍥犳槸涓€娈垫煋鑹查珨
B銆侀爢鍙嶅瓙鏄皣鍩哄洜鎻忚堪鐐轰竴鍊嬪姛鑳藉柈浣�
C銆佷竴鍊嬪熀鍥犱竴鍊嬮叾闈炲父婧栫⒑鍦拌〃閬斿嚭浜嗗熀鍥犵殑鐗规€�
D銆佷竴鍊嬪熀鍥犲氨鏄竴鍊嬬獊璁婂瓙
9. 鏈€涓嶇┅(w臎n)瀹氱殑 RNA 鏄� ( )
A銆乵RNA B銆乼RNA C銆乺RNA D銆佷笂杩颁笁绋┅(w臎n)瀹氭€у樊鍒ヤ笉澶�
10. 鑸囧瘑纰煎瓙 GAU 灏嶆噳鐨勫弽瀵嗙⒓瀛愭槸 ( )
A銆丆UA B銆両UC C銆丆UI D銆丄TC
11. 鐪熸牳鐢熺墿鎴愮啛 mRNA 5藠绔殑甯藉瓙绲�(ji茅)妲�(g貌u)鏄� ( )
A銆乵7
G5藠
ppp5藠
(m)N B銆乵7
A5藠
ppp5藠
(m)N
C銆乵7
G3藠
ppp5藠
(m)N D銆乵7
A3藠
ppp5藠
(m)N
12. 闂滀簬鐪熸牳鐢熺墿涓嶉€ｇ簩(x霉)鍩哄洜鐨勬晿杩帮紝閷鐨勬槸 ( )
A銆佽〃鏄庡垵濮嬭綁(zhu菐n)閷勭敘(ch菐n)鐗╁繀闋堣鍔犲伐鍚庢墠鍙缈昏
B銆佸洜鐐虹法纰煎簭鍒楀椤瓙琚潪绶ㄧ⒓鐨勫収(n猫i)鍚瓙鎵€鍒嗛殧
C銆佽〃鏄庣湡鏍稿熀鍥犲彲鑳芥湁澶氱ó琛ㄩ仈鐢�(ch菐n)鐗�
D銆佸弽鏄犱簡鐪熸牳鐢熺墿鐨� mRNA 鏄闋嗗弽瀛�
13. RNA 鑱氬悎閰� I 鐨勫姛鑳芥槸 ( )
A銆佽綁(zhu菐n)閷� tRNA 鍜� 5SrRNA B銆佽綁(zhu菐n)閷勮泲鐧借唱(zh矛)鍩哄洜鍜岄儴鍒� snRNA 鍩哄洜
C銆佸彧杞�(zhu菐n)閷� rRNA 鍩哄洜 D銆佽綁(zhu菐n)閷勫绋熀鍥�
14. 杩勪粖鐐烘锛屽湪鐢熺墿鐣屽皻鐒″厖鍒嗚瓑鎿�(j霉)鐨勯亷绋嬫槸 ( )
A銆丏NA鈫扲NA B銆丷NA鈫扲NA C銆丷NA鈫扗NA D銆佽泲鐧借唱(zh矛)鈫扲NA
15. 绱绶氬皪 DNA 鐨勬悕鍌蜂富瑕佹槸 ( )
A銆佸舰鎴愬槯鍟朵簩鑱氶珨 B銆佸紩璧峰牽鍩虹疆鎻�
C銆佸皫鑷村牽鍩虹己澶� D銆佺櫦(f膩)鐢熷牽鍩烘彃鍏�
16. 闂滀簬鍟熷嫊瀛愮殑鎻忚堪锛屽摢涓€闋呮槸姝ｇ⒑鐨�? ( )
A銆乵RNA 闁嬪琚炕璀殑閭ｆ DNA 搴忓垪
B銆佸彲鑳借綁(zhu菐n)閷勪笉鍚岀殑鍩哄洜鐨勯偅娈� DNA 搴忓垪
C銆丷NA 鑱氬悎閰舵渶鍒濊垏 DNA 绲�(ji茅)鍚堢殑閭ｆ DNA 搴忓垪
D銆侀枊濮嬬敓鎴愮殑 mRNA 搴忓垪
17. 鐒＄京绐佽畩鎸囩殑鏄法纰兼皑鍩洪吀鐨勪笁鑱�(li谩n)瀵嗙⒓瀛愯綁(zhu菐n)璁�?y艒u)?( )
A銆乁AG B銆乁GA C銆乁AA D銆佷互涓婇兘鏄�
18. 涓嬪垪鍚勯爡涓�锛屼笉灞簬鐪熸牳鐢熺墿鍩哄洜琛ㄩ仈杞�(zhu菐n)閷勫墠姘村钩瑾�(di脿o)绡€(ji茅)鐨勯亷绋嬫槸 ( )
A銆丷NA 绶ㄨ集 B銆佹煋鑹茶唱(zh矛)涓熷け
C銆佹煋鑹查珨 DNA 鐨勪慨椋惧拰鐣版煋鑹茶唱(zh矛)鍖� D銆佸熀鍥犻噸鎺�
19. 涓嬪垪鍚勯爡涓紝鍝爡涓嶆槸铔嬬櫧璩�(zh矛)鍚堟垚閬庣▼涓墍蹇呴渶鐨�锛�( )
A銆乵RNA B銆佸収(n猫i)璩�(zh矛)缍�(w菐ng) C銆乼RNA D銆佹牳绯栭珨
20. SD (Shine-Dalgarno) 搴忓垪鏄寚 ( )
鍏� 6 闋� 绗� 4 闋�
A銆佸湪 mRNA 鍒嗗瓙鐨勮捣濮嬪瘑纰间笂娓� 8-13 鍊嬫牳鑻烽吀铏曠殑搴忓垪
B銆佸湪 DNA 鍒嗗瓙涓婅綁(zhu菐n)閷勮捣濮嬮粸鍓� 8-13 鍊嬫牳鑻烽吀铏曠殑搴忓垪
C銆�16s rRNA3'绔瘜鍚槯鍟剁殑浜掕搴忓垪
D銆佸暉鍕曞熀鍥犺綁(zhu菐n)閷勭殑鐗瑰緛搴忓垪
21. 闂滀簬閫嗚綁(zhu菐n)閷勪綔鐢ㄧ殑閷鏁樿堪鏄� ( )
A銆佷互 RNA 鐐烘ā鏉垮悎鎴� DNA
B銆侀渶瑕佷竴鍊嬪叿鏈� 3鈥�-OH 鏈鐨勫紩鐗�
C銆佷互 5鈥欌啋 3鈥欐柟鍚戝悎鎴愶紝涔熻兘 3鈥欌啋 5鈥欐柟鍚戝悎鎴�
D銆佷互 dNTP 鐐哄簳鐗�
22. 浠ヤ笅涓嶈兘瀵︾従(xi脿n)鍩哄洜鏁查櫎 (Gene Knockout) 鐨勬妧琛撴槸 ( )
A銆丷NAi 骞叉摼 B銆乀-DNA 鎻掑叆澶辨椿
C銆乀ALEN 闈跺悜鏍搁吀閰舵妧琛� D銆丆RISPR/Cas9 鍩哄洜绶ㄨ集鎶€琛�
23. 浠ヤ笅鍚勭ó鍙畩鍓帴鏂瑰紡涓笉鍙兘鐧�(f膩)鐢熺殑鏄� ( )
A銆佸椤瓙璺抽亷 B銆佸収(n猫i)鍚瓙淇濈暀
C銆佹敼璁婂椤瓙閫ｆ帴鐨勯爢搴� D銆佸彲璁婄殑 5鈥插壀鎺ヤ綅榛�
24. 2015 骞磋璨濈埦鍖栧鐛庨爳绲︿簡鐮旂┒ DNA 鎼嶅偡淇京鐨勪笁浣嶇瀛稿锛屼笅鍒楃櫦(f膩)鐝�(xi脿n)涓笉灞簬閫欎笁
浣嶇瀛稿鐮旂┒宸ヤ綔鐨勬槸 ( )
A銆佹牳鑻烽吀鍒囬櫎淇京 B銆佸牽鍩哄垏闄や慨寰�
C銆侀尟閰嶄慨寰� D銆佸悓婧愰噸绲勪慨寰�
25. 鐪熸牳绱拌優鏌撹壊璩�(zh矛)鐨勫熀鏈祼(ji茅)妲�(g貌u)鍠綅鏄� ( )
A銆佺祫铔嬬櫧 B銆佹牳灏忛珨 C銆佹牳蹇冮绮� D銆佽秴铻烘棆绠�
26. 涓嬪垪闂滀簬 MicroRNA (miRNA) 鐨勬弿杩伴尟瑾ょ殑鏄� ( )
A銆乵iRNA 鏄湪鐪熸牳鐢熺墿涓櫦(f膩)鐝�(xi脿n)鐨勪竴椤為潪绶ㄧ⒓ RNA
B銆佹垚鐔熺殑 miRNA 閫氬父鏄敱杓冮暦鐨勫垵绱氳綁(zhu菐n)閷勭墿缍�(j墨ng)涓€绯诲垪鏍搁吀閰跺壀鍒囧姞宸ヨ€屾垚
C銆佹垚鐔� miRNA 澶у皬闀风磩 20-25 鍊嬫牳鑻烽吀
D銆乵iRNA 涓昏閫氶亷鑸� DNA 涓婇澏浣嶉粸鐨勪簰瑁滀綔鐢ㄨ(di脿o)鎺у熀鍥犺〃閬�
27. 涓嬪垪鍝€嬫搷绺卞瓙娌掓湁琛版笡瀛愬簭鍒楋紵 ( )
A銆乼rp 鎿嶇副瀛� B銆乸he 鎿嶇副瀛� C銆乭is 鎿嶇副瀛� D銆乴ac 鎿嶇副瀛�
Lkp1 鏄倽鍜岃厧绱拌優鐢�(ch菐n)鐢熺殑涓€绋壒鐣拌泲鐧借唱(zh矛)锛岃€屽湪蹇冭嚐绱拌優涓娓笉鍒� LKP1 鍩哄洜鐨勮〃閬�銆�
灏� LKP1 鍩哄洜涓婃父 DNA 搴忓垪缃簬缍犺壊鐔掑厜铔嬬櫧鍩哄洜锛圙FP锛変笂娓革紝鍦ㄨ倽鍜岃厧绱拌優涓彲妾㈡脯鍒�
GFP 鐨勮〃閬�锛屽湪蹇冭嚐绱拌優妾㈡脯涓嶅埌 GFP 淇¤櫉銆傚凡鏈夌爺绌惰〃鏄�锛孡KP1 鍩哄洜涓婃父搴忓垪涓殑 A銆�
B銆丆 涓夊€嬭(di脿o)鎺у簭鍒楀弮鑸囦簡閫欑ó琛ㄩ仈妯″紡鐨勮(di脿o)绡€(ji茅)锛岃€� X銆乊銆乑 涓夌ó铔嬬櫧瑾�(di脿o)绡€(ji茅)鍥犲瓙鍙垎鍒ュ皥涓€
鎬х殑鑸� A銆丅銆丆 搴忓垪绲�(ji茅)鍚堛€傜従(xi脿n)閫氶亷灏� A銆丅銆丆 搴忓垪鍒嗗垾閫茶濡備笅鍦栨墍绀虹殑缂哄け绐佽畩锛屼娇寰�
X銆乊銆乑 杞�(zhu菐n)閷勫洜瀛愮殑灏堜竴鎬х祼(ji茅)鍚堝柂澶�锛屼締鐮旂┒ LKP1 鍩哄洜琛ㄩ仈鐨勮(di脿o)鎺ф鐞�銆傝珛鏍规摎(j霉)瀵﹂绲�(ji茅)鏋�
鍜屽湒绀�锛屽洖绛斿晱椤� 28-30锛�
鍏� 6 闋� 绗� 5 闋�
28. 鍝竴鍊嬶紙浜涳級铔嬬櫧鐧�(f膩)鎻簡闃婚亸铔嬬櫧浣滅敤锛� ( )
A銆乆 鍥犲瓙 B銆乊 鍥犲瓙 C銆乑 鍥犲瓙 D銆佷互涓婂潎鏄� E銆佷互涓婂潎涓嶆槸
29. 鍝竴鍊嬶紙浜涳級铔嬬櫧鐧�(f膩)鎻簡婵€娲昏泲鐧戒綔鐢�锛� ( )
A銆乆 鍥犲瓙 B銆乊 鍥犲瓙 C銆乑 鍥犲瓙 D銆佷互涓婂潎鏄� E銆佷互涓婂潎涓嶆槸
30. 鍝竴绋祫绻旂窗鑳炰腑鍥犲瓙 Z 姝ｅ父琛ㄩ仈骞剁祼(ji茅)鍚堝埌 DNA 涓婏紵 ( )
A銆佹墍鏈夌祫绻� B銆佽倽 liver C銆佸績鑷� heart D銆佽厧 kidney E銆佷互涓婂潎涓嶆槸
鍥涖€佺啊绛旈锛�60 鍒嗭級
1. 涓€姊濋暦鐐� 4.2kb 鐨� 绶氭€NA 鐗囨锛屽垎鍒ョ敤EcoRI锛圗锛�銆丳stI锛圥锛夊強EcoRI+PstI锛圗+P锛�
閰跺垏锛岄浕娉崇祼(ji茅)鏋滃鍦栨墍绀�锛孧鐐哄垎瀛愰噺妯欒锛岀闋墍绀虹偤闆绘吵DNA閬风Щ鏂瑰悜銆傝珛鏍规摎(j霉)绲�(ji茅)鏋�
绻嚭瑭茬墖娈甸檺鍒舵€ч叾鍒囦綅榛炵殑鍦栬瓬銆傦紙6鍒嗭級
鍏� 6 闋� 绗� 6 闋�
2. 鍘熸牳鐢熺墿 mRNA 鑸囩湡鏍哥敓鐗� mRNA 鏈変粈涔堜笉鍚屻€傦紙6 鍒嗭級
3. 绲�(ji茅)鍚堝湒绀虹啊杩板熀鍥犲厠闅嗘妧琛撶殑甯歌(gu墨)姝ラ銆傦紙6 鍒嗭級
4. 鐝�(xi脿n)鍒嗛洟浜嗕竴 DNA 鐗囨锛屽彲鑳藉惈鏈変竴鍊嬬窗鑿屽暉鍕曞瓙鍜岀法纰煎鑲界殑鍩哄洜鐨勫墠骞惧€嬪瘑纰煎瓙銆傝┎
鐗囨鐨勫簭鍒楀涓嬶細
1 CCGGCGGTTTTCATTTTGTCGTTGACATCGGACAAGGGGCTCCTATAATA
51 GGTGCGTTAGTGGACACATGATAGTGTCCACTCTG
1锛� 璜嬪湪涓婅堪搴忓垪涓鍑虹窗鑿屽暉鍕曞瓙鐨勪笁鍊嬬壒寰佸簭鍒楀崁(q奴)鍩�銆傦紙3 鍒嗭級
2锛� mRNA 鐨勫簭鍒楃祫鎴愭槸浠€涔�锛熻兘褰㈡垚浠€涔堟ǎ鐨勪簩绱氱祼(ji茅)妲�(g貌u)锛岀躬鍦栬鏄�銆傦紙4 鍒嗭級
5. 浠€涔堟槸鍙畩鍓帴锛堟垨閬告搰鎬у壀鎺ワ級锛熷畠鏈変粈涔堢敓鐗╁鎰忕京锛熻珛鍊熷姪鍦栫ず鑸変緥瑾槑瀹冩槸濡備綍
閫犳垚鍠€嬪熀鍥犵炕璀敘(ch菐n)鐢熷叐鍊嬩笉鍚屽鑲界殑锛燂紙10 鍒嗭級
6. 鑸変緥瑾槑婵€绱犲奖闊垮熀鍥犺〃閬旂殑鍩烘湰妯″紡銆傦紙10 鍒嗭級
7. 鍦ㄥ皪 E. coli 瑾�(di脿o)鎺ц壊姘ㄩ吀鍚堟垚鐨勫急鍖栧瓙锛圓ttenuator锛夌爺绌朵腑锛岀爺绌惰€呴€氶亷灏嶅墠灏庡簭鍒楋紙leader
sequence锛�1锛�2锛�3锛�4 鍗€(q奴)鍩熺殑搴忓垪鍒嗗垾閫茶澶辨椿绐佽畩锛屽彲浠ュ垎鏋愰€欎簺鍗€(q奴)鍩熷湪瑾�(di脿o)鎺ц壊姘ㄩ吀鍚�
鎴愪腑鐨勪綔鐢�銆傚叾涓湁涓€鍊嬬獊璁婃牚锛岃〃鐝�(xi脿n)鐐鸿壊姘ㄩ吀鍚堟垚缂洪櫡鍨�锛屽嵆涓嶇鍩归(y菐ng)鍩轰腑鑹叉皑閰告績搴�
楂樻垨浣�锛岄兘涓嶈兘姝ｅ父杞�(zhu菐n)閷勮壊姘ㄩ吀鍚堟垚鐩搁棞鐨勯叾銆�
1锛� 璜嬬啊杩拌壊姘ㄩ吀鎿嶇副瀛愬墠灏庡簭鍒楃殑绲�(ji茅)妲�(g貌u)鐗归粸鍙婅(di脿o)鎺х祼(ji茅)妲�(g貌u)鍩哄洜琛ㄩ仈鐨勬柟寮�銆傦紙5 鍒嗭級
2锛� 绨¤鍒嗘瀽涓婅堪绐佽畩鏍け娲荤獊璁婁綅榛炲彲鑳戒綅浜庡墠灏庡簭鍒楃殑鍝簺鍗€(q奴)鍩�锛燂紙10 鍒嗭級
鍏嶈铂鑱叉槑锛氭湰鏂囩郴杞�(zhu菐n)杓夎嚜缍�(w菐ng)绲�锛屽鏈変镜鐘�锛岃珛鑱�(li谩n)绯绘垜鍊戠珛鍗冲埅闄わ紝鍙︼細鏈枃鍍呬唬琛ㄤ綔鑰呭€嬩汉瑙€榛�锛岃垏鏈恫(w菐ng)绔欑劇闂�銆傚叾鍘熷壍(chu脿ng)鎬т互鍙婃枃涓櫝杩版枃瀛楀拰鍏�(n猫i)瀹规湭缍�(j墨ng)鏈珯璀夊锛屽皪鏈枃浠ュ強鍏朵腑鍏ㄩ儴鎴栬€呴儴鍒嗗収(n猫i)瀹�銆佹枃瀛楃殑鐪熷鎬�銆佸畬鏁存€�銆佸強鏅傛€ф湰绔欎笉浣滀换浣曚繚璀夋垨鎵胯锛岃珛璁€鑰呭儏浣滃弮鑰冿紝骞惰珛鑷鏍稿鐩搁棞鍏�(n猫i)瀹�銆�
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鏂囩珷鎼滅储
鎮ㄧ従(xi脿n)鍦ㄧ殑浣嶇疆锛� 
