涓湅(gu贸)杈�(n贸ng)绉戦櫌鍩哄洜宸ョ▼姒傝珫鑰冨崥瑭﹂

涓湅(gu贸)杈�(n贸ng)绉戦櫌鍩哄洜宸ョ▼姒傝珫鑰冨崥瑭﹂

2002骞翠腑鍦�(gu贸)杈�(n贸ng)绉戦櫌鍗氬＋鍏ュ(xu茅)鍩哄洜宸ョ▼姒傝珫瑭﹂
涓€銆佺啊(ji菐n)绛旈
1銆佽仛涓欑儻閰拌兒銆佺搳鑴傜硸鍦―NA闆绘吵涓殑鍗€(q奴)鍒ユ槸浠€涔�锛�
2銆佽垑鍑哄嫊(d貌ng)鐗╄綁(zhu菐n)鍩哄洜鐨勫叐绋柟娉�锛屽苟瑾�(shu艒)鏄庡叾鍘熺悊銆�
3銆侀洐鑴哀娉曟脯(c猫)搴忕殑鍘熺悊銆�
4銆佷互鎿崡鑺ユ垨鐜夌背鐐轰緥锛岃(shu艒)鏄庤綁(zhu菐n)搴у瓙妯�(bi膩o)绨芥硶閫�(j矛n)琛屽熀鍥犺綁(zhu菐n)绉荤殑鍘熺悊銆�
5銆丼outhern鍗拌贰鐨勫師鐞嗗強鎳�(y墨ng)鐢�銆�
涓�銆佽│璜栬堪妞嶇墿鍩哄洜宸ョ▼鐮旂┒閫�(j矛n)灞曚互鍙婂湪杈�(n贸ng)妤�(y猫)鐢熺敘(ch菐n)涓婄殑鎰忕京銆�

2001骞翠腑鍦�(gu贸)杈�(n贸ng)绉戦櫌鍗氬＋鍏ュ(xu茅)鍩哄洜宸ョ▼姒傝珫瑭﹂
涓€銆佸悕瑭炶В閲�
1銆侀檺鍒舵€у収(n猫i)鍒囬叾 2銆佸悓瑁傞叾 3銆佹牳閰� 4銆�2渭鐠�(hu谩n) 5銆乭at閬告搰
6銆乀i璩�(zh矛)绮� 7銆乀-DNA 8銆佸悓鍔焧RNA 9銆佸弽缇﹖RNA 10銆佹湁缇╅張
11銆佄变簰瑁�(b菙) 12銆佸熀鍥犳枃搴�(k霉) 13銆乧DNA 14銆佹煋鑹查珨姝ユ煡
浜岋紟绨�(ji菐n)绛旈
1銆佽垑鍏╃ó妞嶇墿鍩哄洜杞�(zhu菐n)绉荤殑鏂规硶?绨�(ji菐n)杩板叾鍘熺悊銆�
2銆丼outhern鍗拌贰鐨勫熀鏈師鐞�锛岄€欑ó鏂规硶鏈変綍鎳�(y墨ng)鐢�銆�
3銆佸櫖鑿岄珨鑸嘽os浣滆級楂旀湁浣曞崁(q奴)鍒ワ紵
4銆丄FLP鐨勫師鐞嗗強鍏舵噳(y墨ng)鐢�
5銆佹櫘閫歅CR鑸嘡APD鏈変綍鍗€(q奴)鍒�锛屼綍璎傛櫘閫歅CR锛�
6銆佷綍璎傞洐鍏冭級楂�锛岀啊(ji菐n)杩板叾绲勮閬�(gu貌)绋嬪強鍏朵綔鐢ㄦ(j墨)鐞�锛�
涓�銆佸垽鏂烽
1銆佺劇(w煤)璜栫敤鍝ó杞�(zhu菐n)鍖栨柟娉曞潎鍙敤PBR322浣滆級楂�
2銆侀€�(j矛n)鍏ョ窗(x矛)鑿岀殑澶栦締(l谩i)DNA涔嬫墍浠ヨ闄嶈В锛屾槸鍥�?y脿n)姒�?x矛)鑿屽彧淇＞鑷韩DNA锛屼笉淇＞澶栦締(l谩i)DNA
3銆佸彧鏈夌矘绮掔鎵嶅彲浠ヨ閫ｆ帴璧蜂締(l谩i)
4銆佺敤鑷韩浣滃紩鐗╁悎鎴愮殑cDNA閺�锛屽線寰€cDNA骞朵笉瀹屾暣

1998骞翠腑鍦�(gu贸)杈�(n贸ng)绉戦櫌鍗氬＋鍏ュ(xu茅)鍩哄洜宸ョ▼姒傝珫瑭﹂
涓€銆佷粈涔堟槸鍩哄洜宸ョ▼锛屽熀鍥犲伐绋嬪湪杈�(n贸ng)妤�(y猫)鐢熺敘(ch菐n)涓婃湁浣曟剰缇�锛�
浜屻€佺啊(ji菐n)绛旓細
1銆佽仛涓欑儻閰拌兒鍑濊啝闆绘吵鍜岀搳鑴傜硸鍑濊啝闆绘吵鎳�(y墨ng)鐢ㄦ湁浣曠壒榛�(di菐n)锛�
2銆佽垑鍏╃ó妞嶇墿鍩哄洜杞�(zhu菐n)绉荤殑鏂规硶?绨�(ji菐n)杩板叾鍘熺悊銆�
3銆侀洐鑴哀娉曟脯(c猫)搴忕殑鍘熺悊
4銆佽綁(zhu菐n)搴у瓙妯�(bi膩o)绨芥硶鍏嬮殕妞嶇墿鍩哄洜鐨勫師鐞�
5銆丼outhern鍗拌贰鐨勫熀鏈師鐞�锛岄€欑ó鏂规硶鏈変綍鎳�(y墨ng)鐢�锛�
6銆佸湪DNA寰�(f霉)鍒堕亷(gu貌)绋嬩腑鏈�(hu矛)褰㈡垚涓€绋京(f霉)鍒堕珨锛坮eplisome锛夌殑绲�(ji茅)妲�(g貌u)锛屽畠鏄敱鍝咕閮ㄥ垎绲勬垚鐨�锛�
7銆丼anger娓�(c猫)搴忔硶鐨勫熀鏈師鐞嗘槸浠€涔�锛�

1999骞翠腑鍦�(gu贸)杈�(n贸ng)绉戦櫌鍗氬＋鍏ュ(xu茅)鍩哄洜宸ョ▼姒傝珫瑭﹂
涓€锛庡悕瑭炶В閲嬶細
1锛嶤DNA 2 Ti璩�(zh矛)绮� 3. 2u鐠�(hu谩n) 4. HAT閬告搰 5 a浜掕(b菙) 6 YAC 7 杞�(zhu菐n)灏�(d菐o) 8 鍩哄洜鏂囧韩(k霉) 9 闄愬埗鎬у収(n猫i)鍒囬叾 10 鏌撹壊楂旀鏌�
浜岋紟鍟�(w猫n)绛旈锛�
1 鑸変緥瑾�(shu艒)鏄庡叐绋鐗╄綁(zhu菐n)鍩哄洜鐨勬柟娉曘€�
2 Phagmid鍜宑osmid浣滅偤杓夐珨鐨勭壒榛�(di菐n)銆�
3 AFLF鍘熺悊鍙婂叾鎳�(y墨ng)鐢�銆�
4 闆欏厓杓夐珨鐨勬蹇靛強妲�(g貌u)寤哄師鐞�銆�
5 Southern鍚稿嵃闆滀氦鐨勫師鐞嗗強鎳�(y墨ng)鐢�銆�

1995骞翠腑鍦�(gu贸)杈�(n贸ng)绉戦櫌鍗氬＋鍏ュ(xu茅)鍩哄洜宸ョ▼姒傝珫瑭﹂鍚�98骞�

1994骞翠腑鍦�(gu贸)杈�(n贸ng)绉戦櫌鍗氬＋鍏ュ(xu茅)鍩哄洜宸ョ▼姒傝珫瑭﹂
1 AP-PCR鍜屾櫘閫歅CR鐨勫崁(q奴)鍒ャ€�
2 鐢ㄤ簬妞嶇墿鍕�(d貌ng)鐗╃殑鍩哄洜杞�(zhu菐n)绉绘柟娉曟湁浣曞崁(q奴)鍒�銆�
3 妞嶇墿鍩哄洜绲勫拰鍕�(d貌ng)鐗╁熀鍥犵祫绲�(ji茅)妲�(g貌u)鏈変綍鍗€(q奴)鍒�銆�
4 鐩墠宸插厠闅嗚綁(zhu菐n)閷勫洜瀛愭湁閭ｄ簺锛岄噰鐢ㄤ粈涔堟柟娉曞厠闅嗐€�
5 RNA鐨勮嚜鎴戝壀鍒囨湁骞剧ó鏂规硶鍏堕亷(gu貌)绋�銆�
6 鐛插緱鏌愪竴鍩哄洜锛屽浣曞緱鐭ュ叾鍔熻兘銆�
7 瑭�(p铆ng)鍍�(ji脿)杞�(zhu菐n)鍩哄洜瀹夊叏鎬с€�
鍩哄洜宸ョ▼鍘熺悊2003骞磋│椤岃€冧簡5鍊�(g猫)鍚嶈瑙ｉ噵锛�20鍒嗭級锛�5鍊�(g猫)鍒ゆ柗椤岋紙10鍒嗭級锛岀啊(ji菐n)绛旈锛�30鍒嗭級锛屽晱(w猫n)绛旈锛�40鍒嗭級銆傚悕瑭炶В閲�銆佸垽鏂烽鎴戝ぇ閮借涓嶆竻浜嗐€�
绨�(ji菐n)绛旓細1. RNAi锛圧NA骞叉秹锛夌殑宸ヤ綔姗�(j墨)鐞嗗拰浣滅敤銆�
2. 闆欒劔姘ф脯(c猫)搴忔硶鐨勫師鐞嗐€�
3. 鍩哄洜鑺墖鐨勭ó椤炲拰宸ヤ綔鍘熺悊銆�
4. PCR鐨勫伐浣滃師鐞�銆�
鍟�(w猫n)绛旓細1. 鐮旂┒DNA鑸囪泲鐧借唱(zh矛)鐩镐簰浣滅敤鐨勬柟娉曟湁骞剧ó銆�
2. 濡備綍鎻愰珮澶栨簮鍩哄洜鐗囨鍦ㄥ師鏍哥窗(x矛)鑳炰腑鐨勮〃閬�(d谩)閲忋€�
3 鏁樿堪鍔熻兘鍩哄洜绲勫(xu茅)鐨勫収(n猫i)瀹硅垏鎰忕京銆�
鍚嶈锛� 鑴堟矕闆诲牬(ch菐ng)闆绘吵娉�
RNA淇濊(h霉)锛圧NA protection锛�
锛�80%鐨勯閮藉嚭鑷€欓噷锛�
1锛� 浠€涔堟槸鍩哄洜宸ョ▼锛熻│杩板湪杈�(n贸ng)妤�(y猫)鐢熺敘(ch菐n)涓婄殑鎰忕京銆�
2锛� 鑱氫笝鐑叞鑳哄嚌鑶犻浕娉宠垏鐡婅剛绯栭浕娉冲湪鎳�(y墨ng)鐢ㄤ腑鏈変綍涓嶅悓鍜岀壒榛�(di菐n)銆�
3锛� 闆欒劔姘ф硶娓�(c猫)搴忕殑鍩烘湰鍘熺悊鏄粈涔�锛�
4锛� SOUTHERN鍗拌贰鐨勫熀鏈師鐞嗗強鍏舵噳(y墨ng)鐢�銆�
5锛� 瑭﹁堪鍏╃ó杞�(zhu菐n)鍩哄洜鏂规硶骞惰(shu艒)鏄庡叾鍘熺悊.
6锛� 闆欏厓杓夐珨鐨勬蹇靛強鍏舵(g貌u)浠跺師鐞�
7锛� AFLP鐨勫師鐞嗗強鍏舵噳(y墨ng)鐢�.
8锛� PLASMID鍜孋OSMID浣滅偤杓夐珨鐨勭壒榛�(di菐n).
9锛� 瑭﹁堪RFLP
10锛� 瑭﹁堪杞�(zhu菐n)搴у瓙妯�(bi膩o)绨芥硶鍏嬮殕鐩熀鍥�
11锛� 瑭﹁堪瀹氫綅鍏嬮殕鎶€琛�(sh霉)
12锛� 瑭﹁堪RNA绶ㄨ集.
13锛� 瑭﹁堪YAC
14锛� 瑭﹁堪鏌撹壊楂旀绉�
15锛� 瑭﹁堪鍩哄洜鑺墖

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1绨�(ji菐n)杩扮湡鏍哥敓鐗╁熀鍥犺〃閬�(d谩)鐨勪富瑕佽(di脿o)鎺х挵(hu谩n)绡€(ji茅)鍜岃泲鐧借唱(zh矛)鍚堟垚鍚庨澏鍚戦亱(y霉n)閫佺殑椤炲瀷
2鐢熺墿澶у垎瀛愪箣闁撶殑鐩镐簰浣滅敤鏄敓鍛界従(xi脿n)璞＄殑鍏烽珨琛ㄧ従(xi脿n)锛岃│浠ヨ泲鐧借唱(zh矛)涔嬮枔浠ュ強铔嬬櫧璩�(zh矛)鑸囨牳閰稿垎瀛愪箣闁撶殑鐩镐簰浣滅敤鐐轰緥鍔犱互瑾�(shu艒)鏄�
3鐪熸牳鑸囧師鏍歌綁(zhu菐n)閷勭殑鍗€(q奴)鍒�
4鐢熺墿鑶滄湁閭ｄ簺涓昏鐨勭敓鐗╁姛鑳芥竻鑸変緥瑾�(shu艒)鏄庤啘铔嬬櫧鍙冭垏閭ｄ簺閲嶈鐨勭敓鍛介亷(gu貌)绋�
5閰惰(di脿o)鎺ф槸浠ｈ瑵瑾�(di脿o)鎺х殑瀵�(sh铆)璩�(zh矛)锛岀偤浠€涔�?閰舵按骞宠(di脿o)绡€(ji茅)灞ゆ
6浣曡瑐铔嬬櫧璩�(zh矛)鐨勭７閰稿寲鍜岃劔纾烽吀鍖�锛岃│浠ュ(sh铆)渚嬭(shu艒)鏄庡叾灏�(du矛)鐢熼暦(zh菐ng)鍜岀櫦(f膩)鑲茬殑瑾�(di脿o)绡€(ji茅)鐨勪綔鐢�

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