涓�?锛熻悕鍗抽W(xu茅)纰╁＋鐮旂┒鐢熷叆瀛�(xu茅)鑰冭│

銆婇伜鍌冲(xu茅)銆嬭€冭│澶х侗

涓€銆佽€冭│绉戠洰鍩烘湰瑕佹眰鍙婇仼鐢ㄨ寖鍦嶆杩�

锛�1锛夋帉鎻￠伜鍌冲(xu茅)锛堝緸鍒嗗瓙銆佺窗(x矛)鑳�銆佸€�(g猫)楂斿埌缇ら珨姘村钩锛夌殑鍩烘湰姒傚康鍜屽師鐞�锛�

锛�2锛夌悊瑙ｉ伜鍌冲(xu茅)鐞嗚珫鍙婂(sh铆)椹�(y脿n)鏂规硶瀛�(xu茅)鐨勯倧杓熀绀�(ch菙)锛�

锛�3锛変簡瑙ｉ伜鍌冲(xu茅)鐨勫悇闋�(xi脿ng)鎶€琛�(sh霉)鍘熺悊鍙婃噳(y墨ng)鐢ㄥ皪璞�锛�

锛�4锛夎兘澶犻€氶亷宸叉帉鎻＄殑鐭ヨ瓨鍜屾柟娉曚締鍒嗘瀽鍙婅В姹哄叿楂旂殑鐢熺墿瀛�(xu茅)鍟忛銆�

浜�銆佽€冭│褰㈠紡鍜岃│鍗风祼(ji茅)妲�(g貌u)

鑰冭│褰㈠紡锛氶枆鍗�锛岀瓎瑭�锛涜€冭│鏅�(sh铆)闁擄細180 鍒嗛悩锛涚附鍒嗭細150 鍒�

瑭﹀嵎绲�(ji茅)妲�(g貌u)锛氬垽鏂烽锛屽柈闋�(xi脿ng)閬告搰椤�銆佺啊绛旈銆侀伜鍌冲(xu茅)瑷�(j矛)绠楅銆佺稖鍚堝垎鏋愰绛�

涓�銆佽€冭│鍏�(n猫i)瀹硅垏瑕佹眰

锛堜竴锛� 缍�(j墨ng)鍏搁伜鍌冲(xu茅)锛堝瓱寰风埦閬哄偝瀛�(xu茅)锛�

1锛庣啛绶存帉鎻″瓱寰风埦鐨勫垎闆㈠畾寰嬪拰鑷敱绲勫悎瀹氬緥鐨勫師鐞嗗拰鐩搁棞(gu膩n)鐨勫熀鏈蹇�

2锛庤兘澶�?q奴)顬搝鍌冲(xu茅)鏁�(sh霉)鎿�(j霉)閫�(j矛n)琛屼竴瀹氱殑绲�(t菕ng)瑷�(j矛)鍒嗘瀽

3锛庝簡瑙ｅ瓱寰风埦閬哄偝鐤剧梾鐨勫師鐞嗗強鍒嗘瀽鎵嬫

4锛庣悊瑙ｇ瓑浣嶅熀鍥狅紙allele锛夌殑姒傚康鍙婁富瑕侀鍒ワ紙缂哄け鍔熻兘/鐛插緱鍔熻兘锛岄’鎬�/闅卞舰

绛夛級

锛堜簩锛� 鏌撹壊楂旈伜鍌冲(xu茅)鑸囩窗(x矛)鑳為伜鍌冲(xu茅)

1. 鐞嗚В绱�(x矛)鑳炲垎瑁傜殑閬庣▼鑸囨剰缇�锛�

2. 浜嗚В鎺屾彙鏈夌挡鍒嗚鑸囨笡鏁�(sh霉)鍒嗚鐨勭暟鍚�锛�

3. 鐞嗚В鏌撹壊楂斿(xu茅)瑾殑鍩烘湰姒傚康鍜屽師鐞嗭紙鍖呮嫭鏌撹壊楂斿湪鏈夌挡鍒嗚鍜屾笡鏁�(sh霉)鍒嗚涓殑

琛岀偤锛�

4. 鎺屾彙鏌撹壊楂斿(xu茅)瑾殑涓昏鍏�(n猫i)瀹�锛�

5. 鎺屾彙鐪熸牳鐢熺墿鏌撹壊楂旂殑绲勮鑸囩祼(ji茅)妲�(g貌u)妯″瀷銆�

绗� 1 闋�锛屽叡 4 闋�

涓婃捣绉戞妧澶у(xu茅)鐢熷懡绉戝(xu茅)鑸囨妧琛�(sh霉)瀛�(xu茅)闄㈢ⅸ澹爺绌剁敓鍏ュ(xu茅)鑰冭│澶х侗

锛堜笁锛夊熀鍥犺垏鍩哄洜绲勭殑绲�(ji茅)妲�(g貌u)鑸囧姛鑳�

1. 鐞嗚В DNA 闆欒灪鏃嬫ā鍨�锛屼互鍙婂叾鍜岄伜鍌冲(xu茅)鐨勫瘑鍒囬棞(gu膩n)绯汇€�

2. 浜嗚В鍩哄洜鐨勫熀鏈畾缇╁拰鏈唱(zh矛)锛岀悊瑙ｅ熀鍥犺垏 DNA 鐨勯棞(gu膩n)绯�锛�

3. 鎺屾彙鐥呮瘨銆佸師鏍�銆佺湡鏍哥敓鐗╁熀鍥犵祫绲�(ji茅)妲�(g貌u)鐗归粸(di菐n)鍜屽姛鑳界殑灏嶆噳(y墨ng)闂�(gu膩n)绯伙紱

4. 鐞嗚В绛変綅鍩哄洜锛坅llele锛夌瓑閬哄偝瀛�(xu茅)姒傚康鑸囧熀鍥犻枔鐨勯棞(gu膩n)绯伙紱

5. 鐞嗚В鍩哄洜瀹舵棌鐨勬蹇�锛�

6. 浜嗚В DNA 鍙婂叏鍩哄洜绲勬脯搴忕殑鍘熺悊鍙婄瓥鐣ャ€�

锛堜笁锛夐伜鍌崇墿璩�(zh矛)鐨勬敼璁�

1. 鎺屾彙绐佽畩鐨勬蹇�銆佺獊璁婇鍨嬪強鍏跺垎瀛愬熀绀�(ch菙)锛�

2. 鎺屾彙鏌撹壊楂斿悇绋獊璁婇鍨嬬殑鐗归粸(di菐n)锛岀櫦(f膩)鐢熺殑姗�(j墨)鍒跺拰閬哄偝鏁堟噳(y墨ng)锛涘苟鑳介€�(j矛n)琛屼竴瀹氱殑

閬哄偝鏁�(sh霉)鎿�(j霉)鍒嗘瀽

3. 浜嗚В鏌撹壊楂旀暩(sh霉)鐩畩鐣扮殑鍩烘湰椤炲瀷锛屽舰鎴愬師鍥犺垏閬哄偝鏁堟噳(y墨ng)锛�

4. 浜嗚В鏌撹壊楂旇畩鐣板湪閫�(j矛n)鍖栦腑鐨勬剰缇╋紱

锛堝洓锛夐伜鍌冲垎鏋�

1. 鐞嗚В鍒╃敤妯″紡鐢熺墿锛岄噰鐢ㄩ伜鍌冲(xu茅)鏂规硶锛堟瘮濡傛鍙嶅悜閬哄偝绡╅伕锛変締鍒嗘瀽鍩哄洜鍔�

鑳界殑鍩烘湰鍘熺悊

2. 鐞嗚В鍩哄洜鍨�(genotype)銆佽〃鍨�(phenotype)銆佸椤巼(penetrance)銆佺瓑姒傚康锛�

3. 鐞嗚В鎺屾彙绛変綅鍩哄洜銆佸京(f霉)绛変綅鍩哄洜銆侀潪绛変綅鍩哄洜绛夋蹇�锛�

4. 浜嗚В鍩哄洜绐佽畩鐨勪富瑕侀鍨嬪強瑾樿畩鏂规硶銆�

5. 鐞嗚В閬哄偝浜掕(b菙)妾㈡脯鐨勫師鐞嗗苟鑳藉姞浠ラ亱(y霉n)鐢�

6. 鐔熺反閬�(y霉n)鐢ㄥ熀鍥犵殑閫ｉ帠鑸囦氦鎻涘畾寰嬮€�(j矛n)琛岄噸绲勯牷鐜囩殑瑷�(j矛)绠�锛屾帉鎻′笁榛�(di菐n)娓氦娉曠殑鍘�

鐞嗚垏鎳�(y墨ng)鐢�锛�

7. 鐞嗚В鍩轰簬閫ｉ帠浜ゆ彌閲嶇祫鐨勫湒浣嶅厠闅嗗師鐞�锛屼簡瑙ｉ伜鍌虫嫰鏁�(rescue)鎴栧垎瀛愰伜鍌�

浜掕(b菙)瀵�(sh铆)椹�(y脿n)纰哄畾鍩哄洜鍔熻兘鐨勫師鐞�銆�

8. 鎺屾彙鍚屾簮閲嶇祫銆佷綅榛�(di菐n)鐗圭暟閲嶇祫鐨勫師鐞嗚垏鐗归粸(di菐n)锛�

9. 鎺屾彙閬哄偝閲嶇祫浣滃湒锛岀己澶变綔鍦栫殑鍘熺悊鑸囨噳(y墨ng)鐢�锛�

10. 鐞嗚В閬哄偝妯�(bi膩o)瑷樸€佸钩琛℃煋鑹查珨鐨勫師鐞�銆佺壒榛�(di菐n)鑸囨噳(y墨ng)鐢�锛�

绗� 2 闋�锛屽叡 4 闋�

涓�?锛熻悕鍗抽W(xu茅)鐢熷懡绉戝(xu茅)鑸囨妧琛�(sh霉)瀛�(xu茅)闄㈢ⅸ澹爺绌剁敓鍏ュ(xu茅)鑰冭│澶х侗

11. 鎺屾彙杞�(zhu菐n)搴у瓙鑸囬€嗚綁(zhu菐n)搴у瓙鐨勫熀鏈蹇靛強绲�(ji茅)妲�(g貌u)鐗归粸(di菐n)锛岃綁(zhu菐n)搴фā鍨�銆備簡瑙ｅ熀浜庤綁(zhu菐n)搴у瓙

鎶€琛�(sh霉)鐨勪富瑕佺殑鐮旂┒鍒嗘瀽鏂规硶

12. 浜嗚В鑷櫦(f膩)绐佽畩銆佽獦鐧�(f膩)绐佽畩銆佷汉宸ュ悓婧愰噸绲勭瓑鍩哄洜绲勪慨椋炬柟娉曠殑鍒嗗瓙姗�(j墨)鍒讹紱浠�

鍙婂叾鍦ㄥ弽鍚戦伜鍌冲垎鏋愪腑鐨勯亱(y霉n)鐢�

锛堜簲锛夊熀鍥犺〃閬�(d谩)鍜岃(di脿o)鎺�

1. 鐔熺反鎺屾彙涓績娉曞墖锛�

2. 鐞嗚В闋嗗弽瀛愭蹇碉紱

3. 鐞嗚В杞�(zhu菐n)閷勮捣濮�銆佺祩姝㈢殑绲�(ji茅)妲�(g貌u)鐗归粸(di菐n)鍜屼綔鐢ㄦ(j墨)鐞嗭紱浜嗚В鐩搁棞(gu膩n)鐨勮(di脿o)鎺ф(j墨)鍒�

4. 浜嗚В杞�(zhu菐n)閷勯爢寮�銆佸弽寮忎綔鐢ㄥ厓浠剁殑椤炲瀷鍜屽姛鑳斤紱

5. 鎺屾彙 RNA 鍓嶉珨鍔犲伐鐨勫師鐞�銆佷富瑕侀鍨嬭垏鎰忕京

6. 浜嗚В RNA 鍓嶉珨鍙畩鍓垏鐨勪富瑕侀鍨嬭垏鎰忕京銆�

7. 鎺屾彙 mRNA 鐨勭祼(ji茅)妲�(g貌u)鐗归粸(di菐n)鍜屼綔鐢紝浜嗚В缈昏璧峰鍥犲瓙鑸囪泲鐧借唱(zh矛)鍚堟垚璧峰鍙嶆噳(y墨ng)

鐨勮(di脿o)鎺�锛�

8. 浜嗚В琛ㄨ閬哄偝淇＞瑾�(di脿o)鎺х殑涓昏椤炲瀷銆佷綔鐢ㄦ(j墨)鍒惰垏鎰忕京锛�

9. 浜嗚В闈炵法纰� RNA 鐨勪富瑕侀鍨嬭垏瑾�(di脿o)鎺у熀鍥犺〃閬�(d谩)鐨勬(j墨)鍒躲€�

(鍏�) 鍩哄洜宸ョ▼

1. 鎺屾彙 PCR 鎶€琛�(sh霉)鐨勫師鐞�锛涗互鍙� PCR 鍦ㄧ瀛�(xu茅)鐮旂┒涓殑鐢ㄩ€�銆�

2. 鐞嗚В闄愬埗鎬у収(n猫i)鍒囬叾銆丏NA 鍚堟垚閰跺拰閫ｆ帴閰�銆佸弽杞�(zhu菐n)閷勯叾绛夌殑浣滅敤鍘熺悊銆佺敤閫�锛�

3. 鎺屾彙杞�(zhu菐n)鍩哄洜鐨勬蹇�锛屼簡瑙ｅ父瑕嬬殑杞�(zhu菐n)鍩哄洜鎶€琛�(sh霉)鐨勫師鐞嗐€�

4. 浜嗚В鍩哄洜鎵撻澏鎶€琛�(sh霉)锛坓ene targeting锛夌殑鍘熺悊鍜屽湪鍚勭敓鐗╀腑鐨勯亱(y霉n)鐢ㄦ柟娉�

5. 浜嗚В鏂扮殑鍩哄洜绶ㄨ集鎶€琛�(sh霉)鍙婄浉闂�(gu膩n)鎳�(y墨ng)鐢�

锛堜竷锛夋暩(sh霉)閲忔€х媭鐨勯伜鍌冲垎鏋�

1. 鎺屾彙鏁�(sh霉)閲忔€х媭鐨勬蹇靛強鐗瑰緛锛�

绗� 3 闋侊紝鍏� 4 闋�

涓�?锛熻悕鍗抽W(xu茅)鐢熷懡绉戝(xu茅)鑸囨妧琛�(sh霉)瀛�(xu茅)闄㈢ⅸ澹爺绌剁敓鍏ュ(xu茅)鑰冭│澶х侗

2. 浜嗚В澶氬熀鍥�?q奴)W瑾殑鍩烘湰姒傚康

3. 鐞嗚В閬哄偝鐜囩殑姒傚康鍙婄浉闂�(gu膩n)瑷�(j矛)绠楁柟娉�锛�

4. 浜嗚В杩戜氦鐨勬蹇�锛屾帉鎻¤繎浜ょ郴鏁�(sh霉)鐨勮▓(j矛)绠楁柟娉�

锛堝叓锛夐伜鍌宠垏閫�(j矛n)鍖�

1. 鐞嗚В瀛熷痉鐖剧兢楂斿拰鍩哄洜搴殑姒傚康

2. 鎺屾彙绛変綅鍩哄洜闋荤巼鍜屽熀鍥犲瀷闋荤巼鐨勮▓(j矛)绠楁柟娉曪紙鍖呮嫭绐佽畩鍜岄伕鎿囧皪鍩哄洜闋荤巼鐨�

褰遍熆锛�锛�

3. 浜嗚В Hardy-Weinberg 瀹氬緥鐨勫収(n猫i)瀹�銆佸強褰遍熆 Hardy-Weinberg 骞宠　鐨勫洜绱�锛�

4. 浜嗚В鐗╃ó褰㈡垚鐨勯亷绋嬪拰鏂瑰紡锛�

5. 浜嗚В鏂板熀鍥犵嵅寰楃殑鏂瑰紡浠ュ強鍩哄洜绲勯€�(j矛n)鍖栫殑鏂瑰紡锛�

6. 浜嗚В涓€у(xu茅)瑾殑鍩烘湰姒傚康銆�

鍥�銆佷富瑕佸弮鑰冩浉鐩�

1锛庛€婇伜鍌冲(xu茅)銆嬶紙绗� 3 鐗堬級锛屽妷绁栨礊绛夌法钁�锛岄珮绛夋暀鑲插嚭鐗堢ぞ锛�2013 骞�

2锛嶢N INTRODUCTION TO GENETIC ANALYSIS锛坆y Anthony J.F. Griffiths, Susan

R. Wessler , Sean B. Carroll, John Doebley 锛塒ublisher: W. H. Freeman; 11 edition

绗� 4 闋�锛屽叡 4 闋�