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鍙嬫儏鎻愮ず锛氭湰绔欐彁渚涘叏鍦�400澶氭墍楂樼瓑闄㈡牎鎷涙敹纰╁＋銆佸崥澹爺绌剁敓鍏ュ鑰冭│姝峰勾鑰冪爺鐪熼銆佽€冨崥鐪熼銆佺瓟妗�锛岄儴鍒嗗鏍℃洿鏂拌嚦2012骞�锛�2013骞�锛涘潎鎻愪緵鏀惰不涓嬭級銆� 涓嬭級娴佺▼锛� 鑰冪爺鐪熼 榛炴搳鈥�鑰冪爺瑭﹀嵎鈥濃€濅笅杓�; 鑰冨崥鐪熼 榛炴搳鈥�鑰冨崥瑭﹀嵎搴�鈥� 涓嬭級
婀栧崡甯寖澶у纰╁＋鐮旂┒鐢熷叆瀛歌€冭│鑷懡椤岃€冭│澶х侗
寰╄│绛嗚│绉戠洰 鑰冭│绉戠洰鍚嶇ū锛氬井鐢熺墿瀛�
涓€銆佽€冭│褰㈠紡鑸囪│鍗风祼妲�
1) 鑰冭│鏅傞枔锛�
鑰冭│鏅傞枔鐐� 180 鍒嗛悩銆�
2) 绛旈鏂瑰紡锛氶枆鍗�銆佺瓎瑭�
3) 瑭﹀嵎鍏�(n猫i)瀹圭祼妲�
锛堜竴锛夊井鐢熺墿瀛稿熀绀庣煡璀橀儴鍒� 90%
锛堜簩锛夊井鐢熺墿瀛哥爺绌堕€插睍銆佸嫊鎱�(t脿i)閮ㄥ垎 10%
4) 椤屽瀷绲愭
a: 鍠爡閬告搰椤�锛�10 灏忛锛岃▓ 5.0%
b: 鍒ゆ柗椤�锛� 5 灏忛锛岃▓ 5.0%
c: 濉┖椤岋紝5 灏忛锛岃▓ 5.0%
d: 鍚嶈瑙ｉ噵椤�锛�5 灏忛锛岃▓ 10.0%
e: 绨＄瓟椤�锛�3 灏忛锛岃▓ 15.0%
f: 鍟忕瓟椤�锛�6 灏忛锛岃▓ 60.0%
浜�銆佽€冭│鍏�(n猫i)瀹硅垏鑰冭│瑕佹眰
1銆佺窉璜�
鑰冭│鍏�(n猫i)瀹癸細
锛�1锛夊井鐢熺墿鍦ㄧ敓鐗╃晫鐨勫湴浣嶏細
锛�2锛夊井鐢熺墿瀛稿強鍏跺垎绉戯細
锛�3锛夊井鐢熺墿鐧�(f膩)灞曠啊鍙诧細
锛�4锛夊井鐢熺墿鍦ㄧ敓鍛界瀛镐腑鐨勯噸瑕佹€э細
鑰冭│瑕佹眰锛氫簡瑙ｅ井鐢熺墿鐨勯噸瑕佹€э紝寰敓鐗╄垏浜洪鐨勯棞绯�锛屽井鐢熺墿瀛哥殑鐧�(f膩)灞曞彶锛�
姝峰彶涓婇噸瑕佺瀛稿鐨勪富瑕佽并鐛伙紝鏂颁笘绱€寰敓鐗╁灞曟湜锛屽井鐢熺墿鐨勭壒榛炵瓑銆�
2銆佸師鏍稿井鐢熺墿
鑰冭│鍏�(n猫i)瀹癸細
锛�1锛夌窗鑿岋細鈶犵窗鑿岀殑褰㈡厠(t脿i)鍙婅瀵�锛涒憽绱拌弻鐨勭窗鑳炵祼妲嬶紱鈶㈢窗鑿岀殑绻佹畺鏂瑰紡鍙婄兢
楂斿舰鎱�(t脿i)銆�
锛�2锛夋斁绶氳弻锛� 鈶犳斁绶氳弻鐨勫舰鎱�(t脿i)绲愭锛涒憽鏀剧窔鑿岀殑绻佹畺锛� 鈶㈡斁绶氳弻鐨勮弻钀姐€�
锛�3锛夎棈绱拌弻锛氣憼钘嶇窗鑿岀殑褰㈡厠(t脿i)鑸囩祼妲�锛涒憽钘嶇窗鑿岀殑绻佹畺銆�
锛�4锛夊彜鐢熻弻锛氣憼鍙よ弻绨′粙锛涒憽鍏稿瀷鐨勬サ绔挵(hu谩n)澧冨井鐢熺墿銆�
锛�5锛夊叾浠栧師鏍稿井鐢熺墿锛氣憼绔嬪厠娆℃皬楂�锛涒憽鏀師楂旓紱鈶㈣。鍘熼珨锛涒懀铻烘棆楂斻€�
鑰冭│瑕佹眰锛氭帉鎻″師鏍稿井鐢熺墿锛堢窗鑿�銆佹斁绶氳弻銆佽棈绱拌弻銆佺珛鍏嬫姘忛珨銆佹敮鍘熼珨銆�
琛ｅ師楂�銆佸彜鐢熻弻銆佽灪鏃嬮珨锛夌殑褰㈡厠(t脿i)绲愭鐗归粸锛岀窗鑿岀窗鑳炲鐨勭祼妲嬪拰鍔熻兘锛岄潻铇皬
鏌撹壊姗熺悊锛岀窗鑳炶唱(zh矛)鍜屽収(n猫i)鍚墿椤嗙矑浠ュ強绱拌弻绱拌優鐨勭壒娈婃閫狅紙鑺借優銆佺硸琚拰闉瘺銆�
鑿屾瘺銆佹€ф瘺绛夛級锛涘師鏍稿井鐢熺墿鐨勭箒娈栫壒鎬�銆佽弻钀界壒寰佺瓑銆�
3銆佺湡鏍稿井鐢熺墿
鑰冭│鍏�(n猫i)瀹癸細
锛�1锛夌湡鑿屾杩帮細鈶犵湡鏍稿井鐢熺墿鑸囧師鏍稿井鐢熺墿姣旇純锛涒憽鐪熻弻鍦ㄨ嚜鐒剁晫鐨勫湴浣�锛涒憿
鏈夐棞鐪熻弻鐨勫咕鍊嬪悕瑭�锛涒懀鐮旂┒鐨勬剰缇┿€�
锛�2锛夐叺姣嶈弻锛氣憼閰垫瘝鑿岀殑褰㈡厠(t脿i)绲愭锛涒憽閰垫瘝鑿岀殑绻佹畺锛涒憿閰垫瘝鑿岀殑鍩归(y菐ng)鐗瑰緛锛�
鈶ｅ咕绋父瑕嬬殑閰垫瘝鑿�銆�
锛�3锛夐湁鑿岋細鈶犻湁鑿岀殑褰㈡厠(t脿i)鍜岀祼妲�锛涒憽闇夎弻鐨勭箒娈栨柟寮忓拰绻佹畺绲愭锛涒憿闇夎弻鐨勫煿
椁�(y菐ng)鐗瑰緛锛涒懀闇夎弻鑸囧叾瀹冨井鐢熺墿鐨勬瘮杓�锛涒懁鑸囦汉椤為棞绯诲瘑鍒囩殑骞剧ó闇夎弻銆�
鑰冭│瑕佹眰锛氭帉鎻＄湡鏍稿井鐢熺墿鑸囧師鏍稿井鐢熺墿鐨勪富瑕佸崁(q奴)鍒�銆侀叺姣嶈弻鍜岄湁鑿岀殑褰㈡厠(t脿i)
绲愭銆佺箒娈栨柟寮忓拰鑿岃惤鐗瑰緛锛涙帉鎻＄浉闂滅殑涓€浜涙蹇�銆傜悊瑙ｇ湡鑿屽湪鑷劧鐣岀殑鍦颁綅浠�
鍙婅垏浜洪鐨勯棞绯汇€傜悊瑙ｉ叺姣嶈弻鐢熸椿鍙茬殑涓夌ó椤炲瀷绛�銆�
4銆佺梾姣�
鑰冭│鍏�(n猫i)瀹癸細
锛�1锛夌梾姣掓杩帮細鈶犵梾姣掔殑鐗归粸鍙婂畾缇�锛涒憽鐥呮瘨鐨勫垎椤炲強鍛藉悕銆�
锛�2锛夌梾姣掔殑褰㈡厠(t脿i)绲愭鍜屽寲瀛哥祫鎴愶細鈶犵梾姣掔殑褰㈡厠(t脿i)澶у皬鍜岀祼妲�锛涒憽鐥呮瘨鐨勫寲瀛哥祫
鎴�銆�
锛�3锛夌梾姣掔殑澧炴畺锛氣憼鐥呮瘨鐨勫京鍒�锛涒憽涓€姝ョ敓闀锋洸绶氾紱鈶㈢儓鎬у櫖鑿岄珨鍜屾韩鍜屽櫖鑿�
楂�锛涒懀闈炲娈栨€ф劅鏌�銆�
锛�4锛夌梾姣掑鐮旂┒鐨勫熀鏈柟娉曪細鈶犵梾姣掔殑鍩归(y菐ng)锛涒憽鐥呮瘨鐨勯憭瀹氾紱鈶㈢梾姣掑畾閲忕殑骞�
鍊嬫蹇�銆�
锛�5锛変簽鐥呮瘨鍥犲瓙锛氣憼椤炵梾姣�锛涒憽琛�(w猫i)鏄熺梾姣掞紱鈶㈣(w猫i)鏄� RNA锛涒懀鏈婄梾姣掋€�
锛�6锛夌梾姣掕垏瀵﹁笎锛氣憼鐥呮瘨鑸囦汉椤炲仴搴�锛涒憽鐥呮瘨鑸囪静(n贸ng)妤�(y猫)锛涒憿鐥呮瘨鑸囩櫦(f膩)閰靛伐妤�(y猫)锛�
鈶ｇ梾姣掑湪鍩哄洜宸ョ▼涓殑鎳夌敤銆�
锛�7锛夋柊鑸堢梾姣掞細鈶犳柊鑸堢梾姣掔殑鐣屽畾锛涒憽鏂拌垐鐥呮瘨鐨勪締婧愬強鍑虹従(xi脿n)鐨勫師鍥�锛涒憿鏂拌垐
鐥呮瘨鐨勯爯闃茶垏鎺у埗绛栫暐銆�
鑰冭│瑕佹眰锛氫簡瑙ｇ梾姣掑鐨勭爺绌跺皪鐢熷懡绉戝鐨勯噸澶ц并鐛�銆傛帉鎻＄梾姣掍綔鐐虹壒娈婂舰
寮忕殑鐢熷懡鐨勭壒榛�銆佸寲瀛哥祫鎴�銆佺祼妲嬶紝鐞嗚В鐥呮瘨寰╁埗閬庣▼鍙婇嚌灏嶇梾姣掑京鍒堕亷绋嬬殑姣�
鍊嬬挵(hu谩n)绡€(ji茅)閫茶钘ョ墿瑷▓鐨勭悊璜栧熀绀�锛屼簡瑙ｇ梾姣掔爺绌堕€插睍鍙婄梾姣掕垏鍏朵粬鐢熺墿涔嬮枔鐨勭浉
浜掗棞绯荤瓑绛夈€�
5銆佸井鐢熺墿鐨勭嚐椁�(y菐ng)鍜屽煿椁�(y菐ng)鍩�
鑰冭│鍏�(n猫i)瀹癸細
锛�1锛夊井鐢熺墿鐕熼(y菐ng)鍏绱犲強鍏跺姛鑳斤細鈶犵⒊婧愶紱鈶℃爱婧�锛涒憿鑳芥簮锛涒懀鐢熼暦鍥犲瓙锛涒懁
鐒℃楣斤紱鈶ユ按銆�
锛�2锛夊井鐢熺墿鐨勭嚐椁�(y菐ng)椤炲瀷锛氣憼鍏夎兘鑷(y菐ng)锛涒憽鍏夎兘鐣伴(y菐ng)锛涒憿鍖栬兘鑷(y菐ng)锛涒懀鍖栬兘鐣伴(y菐ng)銆�
锛�3锛夌嚐椁�(y菐ng)鐗╄唱(zh矛)閫插叆寰敓鐗╃窗鑳烇細鈶犺嚜鐢辨摯鏁�锛涒憽鍗�(xi茅)鍔╂摯鏁ｏ紱鈶富鍕曢亱杓�锛涒懀鍩�
鍦樿綁(zhu菐n)浣嶃€�
锛�4锛夊煿椁�(y菐ng)鍩猴細鈶犲煿椁�(y菐ng)鍩洪厤鍒剁殑鍩烘湰鍘熷墖锛涒憽鍩归(y菐ng)鍩虹ó椤炲強鍏舵噳鐢�銆�
鑰冭│瑕佹眰锛氭帉鎻″悇椤炵嚐椁�(y菐ng)鐗╄唱(zh矛)鍦ㄥ井鐢熺墿绱拌優涓墍璧风殑浣滅敤锛涚悊瑙ｅ苟鎺屾彙鍚勭ó
鐕熼(y菐ng)椤炲瀷鍙婂叾鐗归粸锛涙帉鎻″悇绋嚐椁�(y菐ng)鐗╄唱(zh矛)閫插嚭绱拌優鐨勬柟寮忓強鍏剁壒榛烇紱浜嗚В鍩归(y菐ng)鍩洪厤
鍒跺師鍓�锛岀悊瑙ｅ悇绋煿椁�(y菐ng)鍩哄湪瀵﹂殯涓殑鎳夌敤銆�
6銆佸井鐢熺墿鐨勪唬璎�
鑰冭│鍏�(n猫i)瀹癸細
锛�1锛夊井鐢熺墿鐨勮兘閲忎唬璎濓細鈶犲寲鑳界暟椁�(y菐ng)寰敓鐗╃殑鐢熺墿姘у寲鑸囩敘(ch菐n)鑳�锛涒憽鍖栬兘鑷(y菐ng)寰�
鐢熺墿鐨勭敓鐗╂哀鍖栬垏鐢�(ch菐n)鑳斤紱鈶㈠厜鑳藉井鐢熺墿鐨勮兘閲忎唬璎�锛涒懀鑷(y菐ng)寰敓鐗╁皪浜屾哀鍖栫⒊鐨�
鍥哄畾銆�
锛�2锛夊井鐢熺墿鐗规湁鐨勫悎鎴愪唬璎濓細鈶� 鐢熺墿鍥烘爱浣滅敤锛涒憽 鑲借仛绯栫殑鍚堟垚銆�
锛�3锛夊井鐢熺墿浠ｈ瑵鐨勮(di脿o)绡€(ji茅)锛氣憼閰跺悎鎴愮殑瑾�(di脿o)绡€(ji茅)锛涒憽閰舵椿鎬х殑瑾�(di脿o)绡€(ji茅)銆�
鑰冭│瑕佹眰锛氭帉鎻″井鐢熺墿鐨勭敘(ch菐n)鑳戒唬璎濓紙鐢熺墿姘у寲锛岀暟椁�(y菐ng)寰敓鐗╁拰鑷(y菐ng)寰敓鐗╃殑
鐢熺墿姘у寲鐗瑰緛锛岃兘閲忚綁(zhu菐n)鎻�锛屽厜鑳借綁(zhu菐n)鎻涘井鐢熺墿椤炲瀷鍙婄壒榛烇級锛屽井鐢熺墿鐨勮€楄兘浠ｈ瑵锛堜簩
姘у寲纰崇殑鍥哄畾鍜屽悓鍖�锛岀敓鐗╁浐姘�锛岃偨鑱氱硸鐨勫悎鎴愮瓑锛�锛屽井鐢熺墿浠ｈ瑵鐨勮(di脿o)绡€(ji茅)绛夌浉闂�
鐭ヨ瓨銆傜悊瑙ｅ寲鑳借嚜椁�(y菐ng)寰敓鐗╁湪瀹屽叏鐒℃鐠�(hu谩n)澧冪敓闀蜂唬璎濈殑鍘熺悊鍙婃剰缇╃瓑銆�
7銆佸井鐢熺墿鐨勭敓闀峰強鍏舵帶鍒�
鑰冭│鍏�(n猫i)瀹癸細
锛�1锛夊井鐢熺墿鐢熼暦鐨勭爺绌舵柟娉曪細 鈶� 寰敓鐗╃殑绱斿煿椁�(y菐ng)锛涒憽 寰敓鐗╃殑鍩归(y菐ng)鏂规硶锛�
鈶� 寰敓鐗╃殑鍚屾鍩归(y菐ng)锛涒懀 寰敓鐗╃殑閫ｇ簩(x霉)鍩归(y菐ng)锛涒懁 鐢熼暦绻佹畺鐨勬脯瀹氭柟娉�銆�
锛�2锛夊井鐢熺墿鐨勭敓闀疯(gu墨)寰嬶細鈶犲井鐢熺墿鐨勫€嬮珨鐢熼暦锛涒憽寰敓鐗╃殑缇ら珨鐢熼暦銆�
锛�3锛夌挵(hu谩n)澧冨洜绱犲皪寰敓鐗╃敓闀风殑褰遍熆锛氣憼婧害锛涒憽pH锛涒憿姘ф埃锛涒懀鐕熼(y菐ng)鐗╄唱(zh矛)銆�
锛�4锛夊井鐢熺墿鐢熼暦鐨勬帶鍒讹細鈶犵墿鐞嗘柟娉曠殑鎺у埗锛涒憽鎺у埗鏈夊寰敓鐗╃殑鍖栧鍥犵礌銆�
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浜嗚В骞舵帉鎻″井鐢熺墿鐨勭兢楂旂敓闀疯(gu墨)寰嬪強鍏跺湪瀵﹂殯涓殑鎳夌敤锛屼簡瑙ｇ湡鑿岀殑缇ら珨鐢熼暦鑸�
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8銆佸井鐢熺墿鐨勯伜鍌宠垏璁婄暟
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鐨勮摤鍕冪櫦(f膩)灞曘€�
锛�2锛夊井鐢熺墿鐨勯伜鍌崇墿璩�(zh矛)锛氣憼寰敓鐗╃殑鏍告煋鑹查珨锛涒憽鍩哄洜绲勬脯搴忔妧琛擄紱鈶㈠井鐢熺墿
鐨勬煋鑹查珨澶栭伜鍌冲洜瀛�銆�
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锛�7锛夊熀鍥犲伐绋嬪拰鐢熺墿鍚堟垚瀛革細
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鐨勫井鐢熺墿锛涒懀浜哄強鍕曟鐗╅珨涓婄殑寰敓鐗�锛涒懁宸ヨ静(n贸ng)妤�(y猫)鐢�(ch菐n)鍝佷腑鐨勫井鐢熺墿锛涒懃妤电鐠�(hu谩n)澧�
涓殑寰敓鐗┿€�
锛�2锛夊井鐢熺墿鑸囩敓鐗╃挵(hu谩n)澧冪殑鐩镐簰闂滅郴锛氣憼浜掔敓锛涒憽鍏辩敓锛涒憿鎷姉锛涒懀瀵勭敓锛涒懁绔�
鐖�锛涒懃鎹曢銆�
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鈶�纾峰惊鐠�(hu谩n)銆�
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鐞嗭紱鈶㈡薄鏌撶挵(hu谩n)澧冪殑鐢熺墿淇京锛涒懀寰敓鐗╃殑鐠�(hu谩n)澧冪洠(ji膩n)娓€�
鑰冭│瑕佹眰锛氭帉鎻″苟浜嗚В寰敓鐗╁湪鑷劧鐣岀殑鍒嗗竷瑕�(gu墨)寰�锛岀敓鐗╃挵(hu谩n)澧冧腑寰敓鐗╄垏鐢�
鐗╁洜瀛愮殑闂滅郴锛屽井鐢熺墿鍦ㄨ嚜鐒剁晫纰�銆佹爱銆佺～銆佺７杞�(zhu菐n)鍖栦腑鐨勪綔鐢紱寰敓鐗╁湪鐠�(hu谩n)澧冧繚
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10銆佸井鐢熺墿鍦ㄥ闅涗腑鐨勬噳鐢�
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寰敓鐗╋紱鈶㈡薄姘寸敓鐗╄檿鐞�锛涒懀鐢熺墿淇京鎶€琛�锛涒懁寰敓鐗╁湪鐠�(hu谩n)澧冧腑鐨勫叾浠栨噳鐢ㄣ€�
锛�2锛夊井鐢熺墿鍦ㄧ櫦(f膩)閰靛伐妤�(y猫)涓殑鎳夌敤锛氣憼寰敓鐗╃櫦(f膩)閰佃垏鐧�(f膩)閰靛伐绋�锛涒憽寰敓鐗╃櫦(f膩)閰电殑
鎳夌敤銆�
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鈶㈠井鐢熺墿鐢�(ch菐n)鐢熺殑闄嶈鑴傝棩鐗�锛涒懀鍒╃敤寰敓鐗╃殑杞�(zhu菐n)鍖栦綔鐢ㄣ€�
锛�4锛夊井鐢熺墿鍦ㄨ静(n贸ng)妤�(y猫)涓殑鎳夌敤锛氣憼寰敓鐗╄偉鏂�锛涒憽寰敓鐗╄静(n贸ng)钘�锛涒憿寰敓鐗╁湪鍏朵粬
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浜嗚В寰敓鐗╁湪瀵﹂殯涓噳鐢ㄧ殑鍩烘湰鏂瑰紡锛屽鑿岄珨銆佷唬璎濈敘(ch菐n)鐗┿€佸井鐢熺墿杞�(zhu菐n)鍖�銆佸熀鍥犺硣
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11銆佸偝鏌撹垏鍏嶇柅
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鈶㈢挵(hu谩n)澧冩浠跺皪鐥呭師寰敓鐗╄嚧鐥呮€х殑褰遍熆銆�
锛�2锛夊厤鐤櫒瀹樺拰鍏嶇柅绱拌優锛氣憼鍏嶇柅鍣ㄥ畼锛涒憽鍏嶇柅绱拌優銆�
锛�3锛夐潪鐗圭暟鎬у厤鐤細 鈶犲睆闅滅祼妲�锛涒憽闈炵壒鐣版€у厤鐤窗鑳�锛涒憿楂旀恫鍥犵礌锛涒懀鐐庣櫏
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锛�5锛夊厤鐤噳绛旂殑鐥呯悊鍙嶆噳锛氣憼瓒呮晱鍙嶆噳锛涒憽鑷韩鍏嶇柅??锛涒憿鐛插緱鎬у厤鐤己闄风稖
鍚堢櫏銆�
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12銆佸井鐢熺墿鐨勫垎椤�
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鍠厓鐨勫懡鍚�锛涒懀绱拌弻鍒嗛鍜岀窗鑿岄憭瀹氭墜鍐娿€�
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鍩哄洜鍙婄壒榛�锛涒憿16Sr RNA 閬╃敤浜庡垎椤為憭瀹氬拰绯荤当(t菕ng)鐧�(f膩)鑲插鍒嗘瀽锛涒懀绯荤当(t菕ng)鐧�(f膩)鑲插鍒�
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浜嗚В寰敓鐗╁垎椤炵殑甯歌(gu墨)鍒嗛娉�銆佺敓鍖栫壒寰佸垎椤炴硶鍜屾暩(sh霉)鍊煎垎椤炴硶銆傞噸榛炴帉鎻￠伜鍌崇壒
寰佸垎椤炴硶锛屽挨鍏舵槸鏍规摎(j霉) 16Sr RNA 鍩哄洜閫茶鍒嗛鐨勫師鐞嗗拰鎰忕京銆備簡瑙ｆ渶鏂板垎瀛愮敓
鐗╁鎵嬫鍦ㄥ井鐢熺墿鍒嗛閼掑畾涓熀鏈師鐞嗗拰鎳夌敤锛屽 RLFP,RAPD 绛�銆�
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鏂囩珷鎼滅储
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